Klebsiella pneumoniae subsp. ozaenae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella; Klebsiella pneumoniae

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A377Z5S6|A0A377Z5S6_KLEPO Periplasmic hemin-binding protein OS=Klebsiella pneumoniae subsp. ozaenae OX=574 GN=hmuT_1 PE=4 SV=1
MM1 pKa = 7.67NIGLFYY7 pKa = 10.91GSSTCYY13 pKa = 10.49TEE15 pKa = 3.84MAAEE19 pKa = 5.12KK20 pKa = 10.0IRR22 pKa = 11.84DD23 pKa = 3.65IIGPEE28 pKa = 4.02LVTLHH33 pKa = 6.12NLKK36 pKa = 10.49DD37 pKa = 3.83DD38 pKa = 4.23SPALMSQYY46 pKa = 11.27DD47 pKa = 3.97VLILGIPTWDD57 pKa = 3.26FGEE60 pKa = 4.22IQEE63 pKa = 4.44DD64 pKa = 4.06WEE66 pKa = 4.5AVWDD70 pKa = 4.34QLDD73 pKa = 3.73TLNLEE78 pKa = 4.43GKK80 pKa = 9.65IVALYY85 pKa = 11.09GMGDD89 pKa = 3.29QLGYY93 pKa = 11.02GEE95 pKa = 4.87WFLDD99 pKa = 3.74ALGMLHH105 pKa = 7.54DD106 pKa = 4.49KK107 pKa = 10.83LATKK111 pKa = 10.04GVKK114 pKa = 9.5FVGYY118 pKa = 9.0WPTEE122 pKa = 4.03GYY124 pKa = 10.61EE125 pKa = 4.19FTSPKK130 pKa = 10.02PVIADD135 pKa = 3.41GQLFVGLALDD145 pKa = 3.64EE146 pKa = 4.38TNQYY150 pKa = 10.89DD151 pKa = 4.3LSDD154 pKa = 3.61EE155 pKa = 5.41RR156 pKa = 11.84IQSWCEE162 pKa = 3.63QILGEE167 pKa = 4.17MAEE170 pKa = 4.4HH171 pKa = 7.09FSS173 pKa = 3.73

Molecular weight: 19.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A378BX62|A0A378BX62_KLEPO Branched-chain amino acid transport system carrier protein OS=Klebsiella pneumoniae subsp. ozaenae OX=574 GN=brnQ PE=3 SV=1
MM1 pKa = 7.42FGGKK5 pKa = 10.0GGLGNLMKK13 pKa = 10.29QAQQMQEE20 pKa = 3.99KK21 pKa = 7.38MQKK24 pKa = 7.25MQEE27 pKa = 4.08EE28 pKa = 4.47IAQLEE33 pKa = 4.52VTGEE37 pKa = 4.05SGAGLVKK44 pKa = 9.63VTINGAHH51 pKa = 6.01NCRR54 pKa = 11.84RR55 pKa = 11.84VEE57 pKa = 4.1SRR59 pKa = 11.84PKK61 pKa = 9.42PAGRR65 pKa = 11.84RR66 pKa = 11.84QRR68 pKa = 11.84DD69 pKa = 3.15ARR71 pKa = 11.84RR72 pKa = 11.84SGGCRR77 pKa = 11.84VQRR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84APYY85 pKa = 9.25RR86 pKa = 11.84RR87 pKa = 11.84DD88 pKa = 3.38PEE90 pKa = 4.71RR91 pKa = 11.84EE92 pKa = 3.71DD93 pKa = 3.28GFRR96 pKa = 11.84FCRR99 pKa = 11.84YY100 pKa = 7.45ATAARR105 pKa = 11.84LQDD108 pKa = 3.66AVLMQTSPLLTQLMEE123 pKa = 4.94ALRR126 pKa = 11.84CLPGVGPKK134 pKa = 9.64SAQRR138 pKa = 11.84MAFTLLQRR146 pKa = 11.84DD147 pKa = 3.41RR148 pKa = 11.84SGGMRR153 pKa = 11.84LAQALTRR160 pKa = 11.84AMSKK164 pKa = 9.73IGHH167 pKa = 6.56CADD170 pKa = 3.21CRR172 pKa = 11.84TFTEE176 pKa = 4.26QEE178 pKa = 3.91VCNICSNPRR187 pKa = 11.84RR188 pKa = 11.84QEE190 pKa = 3.59NGQICVVEE198 pKa = 4.71SPADD202 pKa = 3.14IYY204 pKa = 11.28AIEE207 pKa = 4.11QTGQYY212 pKa = 8.99SGRR215 pKa = 11.84YY216 pKa = 7.12FVLMGTCRR224 pKa = 11.84RR225 pKa = 11.84STASGLMTSGSIAWSNGWRR244 pKa = 11.84RR245 pKa = 11.84SRR247 pKa = 11.84SPRR250 pKa = 3.28

Molecular weight: 27.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6726

0

6726

1452714

29

1649

216.0

23.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.423 ± 0.044

1.157 ± 0.012

5.061 ± 0.025

5.364 ± 0.034

3.746 ± 0.023

7.646 ± 0.036

2.316 ± 0.017

5.461 ± 0.027

3.847 ± 0.028

10.908 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.81 ± 0.016

3.356 ± 0.021

4.763 ± 0.024

4.512 ± 0.032

6.385 ± 0.035

5.835 ± 0.029

5.144 ± 0.024

6.96 ± 0.027

1.605 ± 0.013

2.702 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski