Amino acid dipepetide frequency for Escherichia phage vB_EcoP_PhAPEC5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.676AlaAla: 8.676 ± 1.17
0.772AlaCys: 0.772 ± 0.256
5.133AlaAsp: 5.133 ± 0.488
5.36AlaGlu: 5.36 ± 0.417
2.317AlaPhe: 2.317 ± 0.296
6.995AlaGly: 6.995 ± 0.747
1.09AlaHis: 1.09 ± 0.196
4.497AlaIle: 4.497 ± 0.441
5.769AlaLys: 5.769 ± 0.572
7.586AlaLeu: 7.586 ± 0.865
3.089AlaMet: 3.089 ± 0.535
5.178AlaAsn: 5.178 ± 0.471
2.498AlaPro: 2.498 ± 0.484
4.633AlaGln: 4.633 ± 0.975
3.588AlaArg: 3.588 ± 0.342
5.087AlaSer: 5.087 ± 0.527
6.041AlaThr: 6.041 ± 0.647
5.315AlaVal: 5.315 ± 0.446
0.818AlaTrp: 0.818 ± 0.181
3.361AlaTyr: 3.361 ± 0.346
0.0AlaXaa: 0.0 ± 0.0
Cys
0.545CysAla: 0.545 ± 0.185
0.045CysCys: 0.045 ± 0.041
0.636CysAsp: 0.636 ± 0.193
0.772CysGlu: 0.772 ± 0.227
0.318CysPhe: 0.318 ± 0.13
0.591CysGly: 0.591 ± 0.195
0.227CysHis: 0.227 ± 0.098
0.772CysIle: 0.772 ± 0.237
0.591CysLys: 0.591 ± 0.183
0.636CysLeu: 0.636 ± 0.195
0.409CysMet: 0.409 ± 0.184
0.545CysAsn: 0.545 ± 0.197
0.363CysPro: 0.363 ± 0.152
0.227CysGln: 0.227 ± 0.118
0.273CysArg: 0.273 ± 0.116
0.591CysSer: 0.591 ± 0.167
0.5CysThr: 0.5 ± 0.188
0.681CysVal: 0.681 ± 0.194
0.182CysTrp: 0.182 ± 0.091
0.318CysTyr: 0.318 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
5.042AspAla: 5.042 ± 0.569
0.908AspCys: 0.908 ± 0.274
3.18AspAsp: 3.18 ± 0.375
3.816AspGlu: 3.816 ± 0.496
2.635AspPhe: 2.635 ± 0.372
3.043AspGly: 3.043 ± 0.314
0.908AspHis: 0.908 ± 0.212
4.179AspIle: 4.179 ± 0.516
3.225AspLys: 3.225 ± 0.357
4.769AspLeu: 4.769 ± 0.429
1.862AspMet: 1.862 ± 0.322
2.907AspAsn: 2.907 ± 0.265
2.453AspPro: 2.453 ± 0.318
1.953AspGln: 1.953 ± 0.342
2.271AspArg: 2.271 ± 0.264
3.679AspSer: 3.679 ± 0.394
3.952AspThr: 3.952 ± 0.386
3.725AspVal: 3.725 ± 0.389
0.772AspTrp: 0.772 ± 0.182
2.317AspTyr: 2.317 ± 0.434
0.0AspXaa: 0.0 ± 0.0
Glu
7.041GluAla: 7.041 ± 0.855
0.409GluCys: 0.409 ± 0.153
3.952GluAsp: 3.952 ± 0.494
5.814GluGlu: 5.814 ± 0.692
2.544GluPhe: 2.544 ± 0.329
3.361GluGly: 3.361 ± 0.448
0.772GluHis: 0.772 ± 0.207
3.225GluIle: 3.225 ± 0.341
3.543GluLys: 3.543 ± 0.53
5.678GluLeu: 5.678 ± 0.517
2.044GluMet: 2.044 ± 0.29
3.134GluAsn: 3.134 ± 0.435
2.953GluPro: 2.953 ± 0.393
3.134GluGln: 3.134 ± 0.423
1.862GluArg: 1.862 ± 0.29
3.316GluSer: 3.316 ± 0.356
2.998GluThr: 2.998 ± 0.352
4.769GluVal: 4.769 ± 0.587
0.863GluTrp: 0.863 ± 0.203
2.725GluTyr: 2.725 ± 0.341
0.0GluXaa: 0.0 ± 0.0
Phe
2.907PheAla: 2.907 ± 0.342
0.5PheCys: 0.5 ± 0.172
2.589PheAsp: 2.589 ± 0.357
1.772PheGlu: 1.772 ± 0.216
1.136PhePhe: 1.136 ± 0.307
2.725PheGly: 2.725 ± 0.325
0.363PheHis: 0.363 ± 0.103
2.998PheIle: 2.998 ± 0.52
1.817PheLys: 1.817 ± 0.358
2.771PheLeu: 2.771 ± 0.344
1.635PheMet: 1.635 ± 0.307
2.635PheAsn: 2.635 ± 0.469
1.09PhePro: 1.09 ± 0.228
1.544PheGln: 1.544 ± 0.301
1.908PheArg: 1.908 ± 0.293
2.089PheSer: 2.089 ± 0.324
2.589PheThr: 2.589 ± 0.371
2.271PheVal: 2.271 ± 0.365
0.363PheTrp: 0.363 ± 0.134
1.317PheTyr: 1.317 ± 0.206
0.0PheXaa: 0.0 ± 0.0
Gly
5.587GlyAla: 5.587 ± 0.617
0.863GlyCys: 0.863 ± 0.232
3.361GlyAsp: 3.361 ± 0.381
3.543GlyGlu: 3.543 ± 0.303
3.089GlyPhe: 3.089 ± 0.423
3.77GlyGly: 3.77 ± 0.471
1.226GlyHis: 1.226 ± 0.206
4.043GlyIle: 4.043 ± 0.489
5.678GlyLys: 5.678 ± 0.531
5.405GlyLeu: 5.405 ± 0.579
2.135GlyMet: 2.135 ± 0.295
4.633GlyAsn: 4.633 ± 0.455
0.818GlyPro: 0.818 ± 0.192
2.362GlyGln: 2.362 ± 0.357
2.544GlyArg: 2.544 ± 0.482
5.405GlySer: 5.405 ± 0.553
4.497GlyThr: 4.497 ± 0.461
4.86GlyVal: 4.86 ± 0.516
1.045GlyTrp: 1.045 ± 0.27
2.953GlyTyr: 2.953 ± 0.42
0.0GlyXaa: 0.0 ± 0.0
His
1.272HisAla: 1.272 ± 0.239
0.273HisCys: 0.273 ± 0.113
0.999HisAsp: 0.999 ± 0.22
1.226HisGlu: 1.226 ± 0.256
0.5HisPhe: 0.5 ± 0.145
0.591HisGly: 0.591 ± 0.178
0.545HisHis: 0.545 ± 0.212
1.045HisIle: 1.045 ± 0.192
1.181HisLys: 1.181 ± 0.291
1.862HisLeu: 1.862 ± 0.297
0.318HisMet: 0.318 ± 0.1
0.863HisAsn: 0.863 ± 0.192
0.727HisPro: 0.727 ± 0.195
0.5HisGln: 0.5 ± 0.176
0.772HisArg: 0.772 ± 0.184
1.317HisSer: 1.317 ± 0.224
0.908HisThr: 0.908 ± 0.242
0.772HisVal: 0.772 ± 0.182
0.318HisTrp: 0.318 ± 0.15
0.999HisTyr: 0.999 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
4.179IleAla: 4.179 ± 0.387
0.409IleCys: 0.409 ± 0.148
3.816IleAsp: 3.816 ± 0.581
3.634IleGlu: 3.634 ± 0.346
1.862IlePhe: 1.862 ± 0.331
3.27IleGly: 3.27 ± 0.315
1.045IleHis: 1.045 ± 0.235
3.498IleIle: 3.498 ± 0.577
4.088IleLys: 4.088 ± 0.507
3.634IleLeu: 3.634 ± 0.341
1.454IleMet: 1.454 ± 0.23
3.18IleAsn: 3.18 ± 0.35
2.953IlePro: 2.953 ± 0.398
2.544IleGln: 2.544 ± 0.294
2.68IleArg: 2.68 ± 0.318
3.316IleSer: 3.316 ± 0.376
4.679IleThr: 4.679 ± 0.442
3.498IleVal: 3.498 ± 0.48
0.545IleTrp: 0.545 ± 0.19
2.544IleTyr: 2.544 ± 0.35
0.0IleXaa: 0.0 ± 0.0
Lys
6.859LysAla: 6.859 ± 0.497
0.409LysCys: 0.409 ± 0.164
2.635LysAsp: 2.635 ± 0.294
4.588LysGlu: 4.588 ± 0.523
2.407LysPhe: 2.407 ± 0.354
4.043LysGly: 4.043 ± 0.481
1.499LysHis: 1.499 ± 0.325
2.635LysIle: 2.635 ± 0.399
2.907LysLys: 2.907 ± 0.464
6.359LysLeu: 6.359 ± 0.545
2.044LysMet: 2.044 ± 0.294
3.407LysAsn: 3.407 ± 0.328
3.18LysPro: 3.18 ± 0.523
2.953LysGln: 2.953 ± 0.33
2.68LysArg: 2.68 ± 0.341
3.952LysSer: 3.952 ± 0.537
3.997LysThr: 3.997 ± 0.366
3.816LysVal: 3.816 ± 0.35
0.727LysTrp: 0.727 ± 0.229
1.999LysTyr: 1.999 ± 0.376
0.0LysXaa: 0.0 ± 0.0
Leu
7.449LeuAla: 7.449 ± 0.634
0.636LeuCys: 0.636 ± 0.227
4.542LeuAsp: 4.542 ± 0.476
4.224LeuGlu: 4.224 ± 0.48
2.953LeuPhe: 2.953 ± 0.308
6.496LeuGly: 6.496 ± 0.481
1.59LeuHis: 1.59 ± 0.268
3.906LeuIle: 3.906 ± 0.442
4.906LeuLys: 4.906 ± 0.412
6.132LeuLeu: 6.132 ± 0.589
2.635LeuMet: 2.635 ± 0.327
5.178LeuAsn: 5.178 ± 0.407
4.134LeuPro: 4.134 ± 0.474
3.498LeuGln: 3.498 ± 0.484
3.679LeuArg: 3.679 ± 0.425
5.269LeuSer: 5.269 ± 0.552
5.633LeuThr: 5.633 ± 0.401
5.95LeuVal: 5.95 ± 0.548
0.908LeuTrp: 0.908 ± 0.207
2.589LeuTyr: 2.589 ± 0.364
0.0LeuXaa: 0.0 ± 0.0
Met
2.635MetAla: 2.635 ± 0.477
0.136MetCys: 0.136 ± 0.085
1.363MetAsp: 1.363 ± 0.266
2.089MetGlu: 2.089 ± 0.328
0.591MetPhe: 0.591 ± 0.195
1.999MetGly: 1.999 ± 0.278
0.454MetHis: 0.454 ± 0.109
1.681MetIle: 1.681 ± 0.298
2.226MetLys: 2.226 ± 0.306
2.407MetLeu: 2.407 ± 0.373
0.772MetMet: 0.772 ± 0.21
2.226MetAsn: 2.226 ± 0.297
1.09MetPro: 1.09 ± 0.308
1.499MetGln: 1.499 ± 0.285
1.317MetArg: 1.317 ± 0.183
2.862MetSer: 2.862 ± 0.326
1.635MetThr: 1.635 ± 0.313
1.817MetVal: 1.817 ± 0.359
0.363MetTrp: 0.363 ± 0.108
0.727MetTyr: 0.727 ± 0.144
0.0MetXaa: 0.0 ± 0.0
Asn
4.224AsnAla: 4.224 ± 0.599
0.409AsnCys: 0.409 ± 0.161
2.907AsnAsp: 2.907 ± 0.374
2.998AsnGlu: 2.998 ± 0.422
2.135AsnPhe: 2.135 ± 0.314
4.361AsnGly: 4.361 ± 0.529
1.544AsnHis: 1.544 ± 0.299
3.225AsnIle: 3.225 ± 0.34
4.088AsnLys: 4.088 ± 0.379
4.815AsnLeu: 4.815 ± 0.52
1.317AsnMet: 1.317 ± 0.221
3.27AsnAsn: 3.27 ± 0.372
3.361AsnPro: 3.361 ± 0.421
3.588AsnGln: 3.588 ± 0.501
3.134AsnArg: 3.134 ± 0.312
2.907AsnSer: 2.907 ± 0.395
3.997AsnThr: 3.997 ± 0.476
3.361AsnVal: 3.361 ± 0.417
0.818AsnTrp: 0.818 ± 0.214
1.953AsnTyr: 1.953 ± 0.425
0.0AsnXaa: 0.0 ± 0.0
Pro
3.452ProAla: 3.452 ± 0.508
0.273ProCys: 0.273 ± 0.125
2.589ProAsp: 2.589 ± 0.395
3.861ProGlu: 3.861 ± 0.544
1.772ProPhe: 1.772 ± 0.275
2.362ProGly: 2.362 ± 0.312
0.318ProHis: 0.318 ± 0.133
2.362ProIle: 2.362 ± 0.355
1.908ProLys: 1.908 ± 0.255
3.089ProLeu: 3.089 ± 0.356
1.226ProMet: 1.226 ± 0.236
2.044ProAsn: 2.044 ± 0.262
0.863ProPro: 0.863 ± 0.288
1.317ProGln: 1.317 ± 0.272
1.136ProArg: 1.136 ± 0.315
2.635ProSer: 2.635 ± 0.354
3.089ProThr: 3.089 ± 0.361
3.588ProVal: 3.588 ± 0.457
0.681ProTrp: 0.681 ± 0.158
1.317ProTyr: 1.317 ± 0.269
0.0ProXaa: 0.0 ± 0.0
Gln
5.087GlnAla: 5.087 ± 0.92
0.5GlnCys: 0.5 ± 0.191
2.135GlnAsp: 2.135 ± 0.43
3.18GlnGlu: 3.18 ± 0.366
1.772GlnPhe: 1.772 ± 0.239
3.043GlnGly: 3.043 ± 0.607
0.318GlnHis: 0.318 ± 0.119
2.271GlnIle: 2.271 ± 0.244
2.907GlnLys: 2.907 ± 0.459
3.316GlnLeu: 3.316 ± 0.451
1.09GlnMet: 1.09 ± 0.188
2.135GlnAsn: 2.135 ± 0.425
1.136GlnPro: 1.136 ± 0.238
1.726GlnGln: 1.726 ± 0.359
2.135GlnArg: 2.135 ± 0.429
3.089GlnSer: 3.089 ± 0.327
2.816GlnThr: 2.816 ± 0.378
2.816GlnVal: 2.816 ± 0.333
0.454GlnTrp: 0.454 ± 0.136
1.817GlnTyr: 1.817 ± 0.298
0.0GlnXaa: 0.0 ± 0.0
Arg
3.225ArgAla: 3.225 ± 0.466
0.545ArgCys: 0.545 ± 0.169
2.089ArgAsp: 2.089 ± 0.273
3.18ArgGlu: 3.18 ± 0.386
1.544ArgPhe: 1.544 ± 0.245
2.725ArgGly: 2.725 ± 0.318
0.727ArgHis: 0.727 ± 0.194
2.544ArgIle: 2.544 ± 0.44
3.134ArgLys: 3.134 ± 0.312
4.134ArgLeu: 4.134 ± 0.511
1.499ArgMet: 1.499 ± 0.259
2.907ArgAsn: 2.907 ± 0.435
1.454ArgPro: 1.454 ± 0.28
2.271ArgGln: 2.271 ± 0.314
1.681ArgArg: 1.681 ± 0.328
2.317ArgSer: 2.317 ± 0.323
2.271ArgThr: 2.271 ± 0.255
2.271ArgVal: 2.271 ± 0.319
0.454ArgTrp: 0.454 ± 0.132
1.454ArgTyr: 1.454 ± 0.237
0.0ArgXaa: 0.0 ± 0.0
Ser
4.724SerAla: 4.724 ± 0.488
0.409SerCys: 0.409 ± 0.161
3.77SerAsp: 3.77 ± 0.409
4.088SerGlu: 4.088 ± 0.387
2.044SerPhe: 2.044 ± 0.357
5.042SerGly: 5.042 ± 0.473
0.863SerHis: 0.863 ± 0.221
4.043SerIle: 4.043 ± 0.35
3.861SerLys: 3.861 ± 0.579
5.996SerLeu: 5.996 ± 0.578
1.544SerMet: 1.544 ± 0.291
3.18SerAsn: 3.18 ± 0.395
2.68SerPro: 2.68 ± 0.615
2.317SerGln: 2.317 ± 0.431
2.907SerArg: 2.907 ± 0.479
4.088SerSer: 4.088 ± 0.6
3.952SerThr: 3.952 ± 0.529
4.043SerVal: 4.043 ± 0.516
0.863SerTrp: 0.863 ± 0.245
2.044SerTyr: 2.044 ± 0.284
0.0SerXaa: 0.0 ± 0.0
Thr
4.724ThrAla: 4.724 ± 0.7
0.454ThrCys: 0.454 ± 0.166
3.588ThrAsp: 3.588 ± 0.389
3.588ThrGlu: 3.588 ± 0.529
2.953ThrPhe: 2.953 ± 0.255
4.951ThrGly: 4.951 ± 0.49
0.863ThrHis: 0.863 ± 0.239
4.27ThrIle: 4.27 ± 0.508
4.224ThrLys: 4.224 ± 0.434
5.269ThrLeu: 5.269 ± 0.359
1.226ThrMet: 1.226 ± 0.26
4.179ThrAsn: 4.179 ± 0.453
3.361ThrPro: 3.361 ± 0.32
2.317ThrGln: 2.317 ± 0.259
2.68ThrArg: 2.68 ± 0.411
3.77ThrSer: 3.77 ± 0.378
3.634ThrThr: 3.634 ± 0.617
4.815ThrVal: 4.815 ± 0.613
0.727ThrTrp: 0.727 ± 0.194
2.089ThrTyr: 2.089 ± 0.386
0.0ThrXaa: 0.0 ± 0.0
Val
6.541ValAla: 6.541 ± 0.687
0.5ValCys: 0.5 ± 0.144
4.452ValAsp: 4.452 ± 0.501
3.725ValGlu: 3.725 ± 0.42
2.498ValPhe: 2.498 ± 0.377
5.178ValGly: 5.178 ± 0.628
1.499ValHis: 1.499 ± 0.214
2.771ValIle: 2.771 ± 0.501
3.543ValLys: 3.543 ± 0.376
4.815ValLeu: 4.815 ± 0.438
2.18ValMet: 2.18 ± 0.314
4.088ValAsn: 4.088 ± 0.463
2.862ValPro: 2.862 ± 0.385
3.27ValGln: 3.27 ± 0.485
3.316ValArg: 3.316 ± 0.438
3.861ValSer: 3.861 ± 0.584
4.406ValThr: 4.406 ± 0.655
5.042ValVal: 5.042 ± 0.563
0.636ValTrp: 0.636 ± 0.162
2.68ValTyr: 2.68 ± 0.376
0.0ValXaa: 0.0 ± 0.0
Trp
0.954TrpAla: 0.954 ± 0.238
0.227TrpCys: 0.227 ± 0.093
1.181TrpAsp: 1.181 ± 0.295
0.545TrpGlu: 0.545 ± 0.181
0.363TrpPhe: 0.363 ± 0.123
0.681TrpGly: 0.681 ± 0.134
0.318TrpHis: 0.318 ± 0.147
0.454TrpIle: 0.454 ± 0.138
0.636TrpLys: 0.636 ± 0.142
1.317TrpLeu: 1.317 ± 0.268
0.363TrpMet: 0.363 ± 0.1
0.545TrpAsn: 0.545 ± 0.174
0.409TrpPro: 0.409 ± 0.107
0.5TrpGln: 0.5 ± 0.124
0.454TrpArg: 0.454 ± 0.128
0.727TrpSer: 0.727 ± 0.179
0.363TrpThr: 0.363 ± 0.097
1.454TrpVal: 1.454 ± 0.241
0.091TrpTrp: 0.091 ± 0.053
0.454TrpTyr: 0.454 ± 0.154
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.68TyrAla: 2.68 ± 0.285
0.545TyrCys: 0.545 ± 0.182
2.68TyrAsp: 2.68 ± 0.256
2.135TyrGlu: 2.135 ± 0.285
1.59TyrPhe: 1.59 ± 0.317
2.589TyrGly: 2.589 ± 0.369
0.818TyrHis: 0.818 ± 0.222
2.407TyrIle: 2.407 ± 0.42
2.907TyrLys: 2.907 ± 0.447
2.317TyrLeu: 2.317 ± 0.291
0.999TyrMet: 0.999 ± 0.186
2.18TyrAsn: 2.18 ± 0.337
1.499TyrPro: 1.499 ± 0.359
1.59TyrGln: 1.59 ± 0.253
1.408TyrArg: 1.408 ± 0.256
2.18TyrSer: 2.18 ± 0.375
1.726TyrThr: 1.726 ± 0.289
2.998TyrVal: 2.998 ± 0.412
0.409TyrTrp: 0.409 ± 0.127
1.136TyrTyr: 1.136 ± 0.187
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (22016 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski