Amino acid dipepetide frequency for Smittium simulii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.747AlaAla: 3.747 ± 0.054	0.637AlaCys: 0.637 ± 0.015	2.816AlaAsp: 2.816 ± 0.033	3.15AlaGlu: 3.15 ± 0.037	2.306AlaPhe: 2.306 ± 0.032	2.375AlaGly: 2.375 ± 0.038	1.03AlaHis: 1.03 ± 0.019	4.149AlaIle: 4.149 ± 0.042	4.324AlaLys: 4.324 ± 0.044	5.245AlaLeu: 5.245 ± 0.051	0.988AlaMet: 0.988 ± 0.019	3.336AlaAsn: 3.336 ± 0.037	2.127AlaPro: 2.127 ± 0.037	2.433AlaGln: 2.433 ± 0.038	1.974AlaArg: 1.974 ± 0.028	4.784AlaSer: 4.784 ± 0.047	3.249AlaThr: 3.249 ± 0.034	2.928AlaVal: 2.928 ± 0.04	0.394AlaTrp: 0.394 ± 0.012	1.677AlaTyr: 1.677 ± 0.027	0.0AlaXaa: 0.0 ± 0.0
Cys	0.657CysAla: 0.657 ± 0.015	0.22CysCys: 0.22 ± 0.009	0.72CysAsp: 0.72 ± 0.018	0.617CysGlu: 0.617 ± 0.018	0.652CysPhe: 0.652 ± 0.015	0.806CysGly: 0.806 ± 0.017	0.23CysHis: 0.23 ± 0.009	0.971CysIle: 0.971 ± 0.019	1.0CysLys: 1.0 ± 0.019	1.266CysLeu: 1.266 ± 0.023	0.273CysMet: 0.273 ± 0.01	0.901CysAsn: 0.901 ± 0.019	0.625CysPro: 0.625 ± 0.016	0.397CysGln: 0.397 ± 0.012	0.514CysArg: 0.514 ± 0.014	1.319CysSer: 1.319 ± 0.024	0.731CysThr: 0.731 ± 0.017	0.811CysVal: 0.811 ± 0.025	0.138CysTrp: 0.138 ± 0.007	0.504CysTyr: 0.504 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	2.469AspAla: 2.469 ± 0.029	0.68AspCys: 0.68 ± 0.016	2.829AspAsp: 2.829 ± 0.043	2.959AspGlu: 2.959 ± 0.039	3.066AspPhe: 3.066 ± 0.036	2.112AspGly: 2.112 ± 0.033	1.005AspHis: 1.005 ± 0.02	4.77AspIle: 4.77 ± 0.05	3.922AspLys: 3.922 ± 0.046	5.416AspLeu: 5.416 ± 0.048	1.026AspMet: 1.026 ± 0.02	3.978AspAsn: 3.978 ± 0.052	2.175AspPro: 2.175 ± 0.031	2.131AspGln: 2.131 ± 0.029	1.651AspArg: 1.651 ± 0.031	6.218AspSer: 6.218 ± 0.063	3.155AspThr: 3.155 ± 0.037	2.401AspVal: 2.401 ± 0.036	0.466AspTrp: 0.466 ± 0.014	2.26AspTyr: 2.26 ± 0.033	0.001AspXaa: 0.001 ± 0.001
Glu	2.976GluAla: 2.976 ± 0.042	0.795GluCys: 0.795 ± 0.021	2.724GluAsp: 2.724 ± 0.035	3.277GluGlu: 3.277 ± 0.048	2.583GluPhe: 2.583 ± 0.033	1.704GluGly: 1.704 ± 0.025	1.122GluHis: 1.122 ± 0.019	4.618GluIle: 4.618 ± 0.041	5.122GluLys: 5.122 ± 0.053	5.857GluLeu: 5.857 ± 0.051	1.154GluMet: 1.154 ± 0.021	4.942GluAsn: 4.942 ± 0.049	2.015GluPro: 2.015 ± 0.035	2.308GluGln: 2.308 ± 0.036	1.931GluArg: 1.931 ± 0.026	5.228GluSer: 5.228 ± 0.053	3.358GluThr: 3.358 ± 0.039	2.141GluVal: 2.141 ± 0.027	0.478GluTrp: 0.478 ± 0.015	2.432GluTyr: 2.432 ± 0.038	0.001GluXaa: 0.001 ± 0.0
Phe	2.335PheAla: 2.335 ± 0.036	0.768PheCys: 0.768 ± 0.017	2.917PheAsp: 2.917 ± 0.034	2.757PheGlu: 2.757 ± 0.029	2.155PhePhe: 2.155 ± 0.032	2.516PheGly: 2.516 ± 0.037	0.642PheHis: 0.642 ± 0.014	3.255PheIle: 3.255 ± 0.039	3.356PheLys: 3.356 ± 0.036	3.975PheLeu: 3.975 ± 0.038	0.873PheMet: 0.873 ± 0.017	3.222PheAsn: 3.222 ± 0.041	1.494PhePro: 1.494 ± 0.024	1.47PheGln: 1.47 ± 0.025	1.44PheArg: 1.44 ± 0.025	4.606PheSer: 4.606 ± 0.052	2.416PheThr: 2.416 ± 0.03	2.245PheVal: 2.245 ± 0.032	0.449PheTrp: 0.449 ± 0.014	1.771PheTyr: 1.771 ± 0.029	0.001PheXaa: 0.001 ± 0.0
Gly	2.428GlyAla: 2.428 ± 0.039	0.698GlyCys: 0.698 ± 0.016	1.999GlyAsp: 1.999 ± 0.031	1.96GlyGlu: 1.96 ± 0.03	2.21GlyPhe: 2.21 ± 0.034	2.408GlyGly: 2.408 ± 0.057	0.858GlyHis: 0.858 ± 0.022	3.572GlyIle: 3.572 ± 0.044	3.119GlyLys: 3.119 ± 0.038	4.11GlyLeu: 4.11 ± 0.048	0.895GlyMet: 0.895 ± 0.022	2.686GlyAsn: 2.686 ± 0.035	1.303GlyPro: 1.303 ± 0.03	1.357GlyGln: 1.357 ± 0.024	1.582GlyArg: 1.582 ± 0.032	4.024GlySer: 4.024 ± 0.049	2.377GlyThr: 2.377 ± 0.033	2.511GlyVal: 2.511 ± 0.038	0.435GlyTrp: 0.435 ± 0.012	1.904GlyTyr: 1.904 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	0.86HisAla: 0.86 ± 0.019	0.25HisCys: 0.25 ± 0.01	0.886HisAsp: 0.886 ± 0.02	0.854HisGlu: 0.854 ± 0.02	0.922HisPhe: 0.922 ± 0.019	0.791HisGly: 0.791 ± 0.019	0.417HisHis: 0.417 ± 0.013	1.495HisIle: 1.495 ± 0.022	1.48HisLys: 1.48 ± 0.022	1.775HisLeu: 1.775 ± 0.031	0.374HisMet: 0.374 ± 0.011	1.435HisAsn: 1.435 ± 0.026	0.864HisPro: 0.864 ± 0.018	0.808HisGln: 0.808 ± 0.017	0.788HisArg: 0.788 ± 0.016	1.906HisSer: 1.906 ± 0.032	1.106HisThr: 1.106 ± 0.019	0.867HisVal: 0.867 ± 0.018	0.166HisTrp: 0.166 ± 0.009	0.695HisTyr: 0.695 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	3.951IleAla: 3.951 ± 0.045	1.153IleCys: 1.153 ± 0.019	4.864IleAsp: 4.864 ± 0.042	4.74IleGlu: 4.74 ± 0.05	3.286IlePhe: 3.286 ± 0.039	3.101IleGly: 3.101 ± 0.042	1.378IleHis: 1.378 ± 0.024	5.256IleIle: 5.256 ± 0.053	6.237IleLys: 6.237 ± 0.062	6.924IleLeu: 6.924 ± 0.065	1.291IleMet: 1.291 ± 0.023	5.912IleAsn: 5.912 ± 0.062	3.454IlePro: 3.454 ± 0.039	2.924IleGln: 2.924 ± 0.034	2.539IleArg: 2.539 ± 0.03	7.969IleSer: 7.969 ± 0.057	4.126IleThr: 4.126 ± 0.041	3.648IleVal: 3.648 ± 0.041	0.601IleTrp: 0.601 ± 0.016	2.71IleTyr: 2.71 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	4.132LysAla: 4.132 ± 0.045	1.029LysCys: 1.029 ± 0.027	3.955LysAsp: 3.955 ± 0.04	4.112LysGlu: 4.112 ± 0.049	3.064LysPhe: 3.064 ± 0.033	2.384LysGly: 2.384 ± 0.035	1.61LysHis: 1.61 ± 0.026	6.654LysIle: 6.654 ± 0.06	8.098LysLys: 8.098 ± 0.082	7.449LysLeu: 7.449 ± 0.063	1.472LysMet: 1.472 ± 0.024	7.778LysAsn: 7.778 ± 0.09	3.337LysPro: 3.337 ± 0.044	3.084LysGln: 3.084 ± 0.039	3.098LysArg: 3.098 ± 0.039	7.749LysSer: 7.749 ± 0.064	5.082LysThr: 5.082 ± 0.05	3.569LysVal: 3.569 ± 0.038	0.607LysTrp: 0.607 ± 0.018	3.007LysTyr: 3.007 ± 0.035	0.0LysXaa: 0.0 ± 0.0
Leu	5.508LeuAla: 5.508 ± 0.047	1.318LeuCys: 1.318 ± 0.021	5.488LeuAsp: 5.488 ± 0.05	6.251LeuGlu: 6.251 ± 0.07	3.984LeuPhe: 3.984 ± 0.042	4.145LeuGly: 4.145 ± 0.046	1.7LeuHis: 1.7 ± 0.026	6.037LeuIle: 6.037 ± 0.051	7.556LeuLys: 7.556 ± 0.063	8.666LeuLeu: 8.666 ± 0.065	1.679LeuMet: 1.679 ± 0.026	6.945LeuAsn: 6.945 ± 0.061	3.987LeuPro: 3.987 ± 0.042	3.895LeuGln: 3.895 ± 0.047	3.148LeuArg: 3.148 ± 0.038	9.128LeuSer: 9.128 ± 0.074	4.739LeuThr: 4.739 ± 0.043	4.987LeuVal: 4.987 ± 0.051	0.761LeuTrp: 0.761 ± 0.018	3.249LeuTyr: 3.249 ± 0.036	0.001LeuXaa: 0.001 ± 0.001
Met	1.109MetAla: 1.109 ± 0.021	0.258MetCys: 0.258 ± 0.011	1.116MetAsp: 1.116 ± 0.023	1.059MetGlu: 1.059 ± 0.019	0.887MetPhe: 0.887 ± 0.018	1.012MetGly: 1.012 ± 0.019	0.364MetHis: 0.364 ± 0.011	1.25MetIle: 1.25 ± 0.022	1.103MetLys: 1.103 ± 0.018	1.873MetLeu: 1.873 ± 0.027	0.364MetMet: 0.364 ± 0.014	1.182MetAsn: 1.182 ± 0.019	0.788MetPro: 0.788 ± 0.018	0.758MetGln: 0.758 ± 0.022	0.716MetArg: 0.716 ± 0.015	1.756MetSer: 1.756 ± 0.027	0.866MetThr: 0.866 ± 0.017	1.032MetVal: 1.032 ± 0.022	0.152MetTrp: 0.152 ± 0.007	0.633MetTyr: 0.633 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.28AsnAla: 3.28 ± 0.034	0.903AsnCys: 0.903 ± 0.019	3.934AsnAsp: 3.934 ± 0.048	3.752AsnGlu: 3.752 ± 0.055	3.436AsnPhe: 3.436 ± 0.043	2.834AsnGly: 2.834 ± 0.043	1.492AsnHis: 1.492 ± 0.027	6.543AsnIle: 6.543 ± 0.06	6.256AsnLys: 6.256 ± 0.071	6.81AsnLeu: 6.81 ± 0.071	1.459AsnMet: 1.459 ± 0.022	7.191AsnAsn: 7.191 ± 0.093	3.101AsnPro: 3.101 ± 0.034	3.507AsnGln: 3.507 ± 0.043	2.51AsnArg: 2.51 ± 0.033	9.22AsnSer: 9.22 ± 0.086	5.234AsnThr: 5.234 ± 0.053	3.315AsnVal: 3.315 ± 0.039	0.525AsnTrp: 0.525 ± 0.014	2.982AsnTyr: 2.982 ± 0.043	0.001AsnXaa: 0.001 ± 0.001
Pro	2.079ProAla: 2.079 ± 0.029	0.384ProCys: 0.384 ± 0.011	2.034ProAsp: 2.034 ± 0.03	2.799ProGlu: 2.799 ± 0.031	1.591ProPhe: 1.591 ± 0.029	1.781ProGly: 1.781 ± 0.035	0.792ProHis: 0.792 ± 0.017	3.111ProIle: 3.111 ± 0.033	3.318ProLys: 3.318 ± 0.048	3.501ProLeu: 3.501 ± 0.037	0.62ProMet: 0.62 ± 0.019	3.101ProAsn: 3.101 ± 0.04	1.896ProPro: 1.896 ± 0.044	1.713ProGln: 1.713 ± 0.029	1.36ProArg: 1.36 ± 0.023	3.842ProSer: 3.842 ± 0.045	2.716ProThr: 2.716 ± 0.042	2.197ProVal: 2.197 ± 0.033	0.267ProTrp: 0.267 ± 0.01	1.257ProTyr: 1.257 ± 0.026	0.001ProXaa: 0.001 ± 0.0
Gln	2.18GlnAla: 2.18 ± 0.037	0.378GlnCys: 0.378 ± 0.011	2.004GlnAsp: 2.004 ± 0.025	2.197GlnGlu: 2.197 ± 0.032	1.457GlnPhe: 1.457 ± 0.022	1.304GlnGly: 1.304 ± 0.027	0.834GlnHis: 0.834 ± 0.017	3.073GlnIle: 3.073 ± 0.039	3.676GlnLys: 3.676 ± 0.043	3.73GlnLeu: 3.73 ± 0.042	0.745GlnMet: 0.745 ± 0.016	3.889GlnAsn: 3.889 ± 0.048	1.599GlnPro: 1.599 ± 0.032	1.987GlnGln: 1.987 ± 0.043	1.456GlnArg: 1.456 ± 0.031	3.49GlnSer: 3.49 ± 0.039	2.571GlnThr: 2.571 ± 0.034	1.965GlnVal: 1.965 ± 0.029	0.265GlnTrp: 0.265 ± 0.009	1.279GlnTyr: 1.279 ± 0.021	0.001GlnXaa: 0.001 ± 0.0
Arg	2.218ArgAla: 2.218 ± 0.032	0.494ArgCys: 0.494 ± 0.014	1.77ArgAsp: 1.77 ± 0.028	1.869ArgGlu: 1.869 ± 0.031	1.673ArgPhe: 1.673 ± 0.025	1.575ArgGly: 1.575 ± 0.029	0.664ArgHis: 0.664 ± 0.017	2.782ArgIle: 2.782 ± 0.033	2.889ArgLys: 2.889 ± 0.036	3.553ArgLeu: 3.553 ± 0.035	0.731ArgMet: 0.731 ± 0.016	2.387ArgAsn: 2.387 ± 0.034	1.349ArgPro: 1.349 ± 0.028	1.392ArgGln: 1.392 ± 0.024	1.734ArgArg: 1.734 ± 0.029	2.962ArgSer: 2.962 ± 0.041	1.783ArgThr: 1.783 ± 0.024	1.927ArgVal: 1.927 ± 0.026	0.36ArgTrp: 0.36 ± 0.014	1.405ArgTyr: 1.405 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	5.336SerAla: 5.336 ± 0.045	1.194SerCys: 1.194 ± 0.023	5.851SerAsp: 5.851 ± 0.06	5.68SerGlu: 5.68 ± 0.051	4.346SerPhe: 4.346 ± 0.05	4.325SerGly: 4.325 ± 0.051	1.785SerHis: 1.785 ± 0.032	7.61SerIle: 7.61 ± 0.062	8.298SerLys: 8.298 ± 0.073	8.852SerLeu: 8.852 ± 0.069	1.587SerMet: 1.587 ± 0.023	8.384SerAsn: 8.384 ± 0.092	3.512SerPro: 3.512 ± 0.056	4.02SerGln: 4.02 ± 0.041	3.438SerArg: 3.438 ± 0.048	11.254SerSer: 11.254 ± 0.116	6.522SerThr: 6.522 ± 0.055	4.635SerVal: 4.635 ± 0.051	0.724SerTrp: 0.724 ± 0.019	3.074SerTyr: 3.074 ± 0.046	0.001SerXaa: 0.001 ± 0.001
Thr	3.232ThrAla: 3.232 ± 0.034	0.772ThrCys: 0.772 ± 0.019	3.264ThrAsp: 3.264 ± 0.043	3.595ThrGlu: 3.595 ± 0.046	2.551ThrPhe: 2.551 ± 0.035	2.618ThrGly: 2.618 ± 0.036	1.13ThrHis: 1.13 ± 0.02	4.307ThrIle: 4.307 ± 0.042	4.582ThrLys: 4.582 ± 0.047	5.415ThrLeu: 5.415 ± 0.046	0.933ThrMet: 0.933 ± 0.018	4.203ThrAsn: 4.203 ± 0.044	2.891ThrPro: 2.891 ± 0.037	2.514ThrGln: 2.514 ± 0.035	2.252ThrArg: 2.252 ± 0.029	5.708ThrSer: 5.708 ± 0.057	3.783ThrThr: 3.783 ± 0.07	2.994ThrVal: 2.994 ± 0.036	0.463ThrTrp: 0.463 ± 0.014	1.607ThrTyr: 1.607 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	3.024ValAla: 3.024 ± 0.041	0.76ValCys: 0.76 ± 0.017	3.068ValAsp: 3.068 ± 0.035	2.962ValGlu: 2.962 ± 0.036	2.389ValPhe: 2.389 ± 0.032	2.491ValGly: 2.491 ± 0.044	0.867ValHis: 0.867 ± 0.016	3.331ValIle: 3.331 ± 0.037	3.459ValLys: 3.459 ± 0.039	4.722ValLeu: 4.722 ± 0.048	0.906ValMet: 0.906 ± 0.018	3.166ValAsn: 3.166 ± 0.038	2.154ValPro: 2.154 ± 0.031	1.698ValGln: 1.698 ± 0.024	1.653ValArg: 1.653 ± 0.03	4.727ValSer: 4.727 ± 0.041	2.478ValThr: 2.478 ± 0.034	2.825ValVal: 2.825 ± 0.047	0.47ValTrp: 0.47 ± 0.013	1.895ValTyr: 1.895 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	0.517TrpAla: 0.517 ± 0.013	0.13TrpCys: 0.13 ± 0.007	0.542TrpAsp: 0.542 ± 0.018	0.457TrpGlu: 0.457 ± 0.012	0.381TrpPhe: 0.381 ± 0.014	0.398TrpGly: 0.398 ± 0.014	0.146TrpHis: 0.146 ± 0.007	0.654TrpIle: 0.654 ± 0.017	0.596TrpLys: 0.596 ± 0.015	0.774TrpLeu: 0.774 ± 0.018	0.188TrpMet: 0.188 ± 0.008	0.521TrpAsn: 0.521 ± 0.014	0.258TrpPro: 0.258 ± 0.01	0.282TrpGln: 0.282 ± 0.01	0.326TrpArg: 0.326 ± 0.01	0.716TrpSer: 0.716 ± 0.016	0.425TrpThr: 0.425 ± 0.015	0.476TrpVal: 0.476 ± 0.014	0.127TrpTrp: 0.127 ± 0.006	0.275TrpTyr: 0.275 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.63TyrAla: 1.63 ± 0.025	0.532TyrCys: 0.532 ± 0.016	1.908TyrAsp: 1.908 ± 0.028	1.72TyrGlu: 1.72 ± 0.027	1.723TyrPhe: 1.723 ± 0.026	1.759TyrGly: 1.759 ± 0.034	0.673TyrHis: 0.673 ± 0.017	2.625TyrIle: 2.625 ± 0.035	2.881TyrLys: 2.881 ± 0.038	3.259TyrLeu: 3.259 ± 0.04	0.72TyrMet: 0.72 ± 0.016	3.125TyrAsn: 3.125 ± 0.045	1.43TyrPro: 1.43 ± 0.024	1.401TyrGln: 1.401 ± 0.023	1.406TyrArg: 1.406 ± 0.027	3.819TyrSer: 3.819 ± 0.053	2.099TyrThr: 2.099 ± 0.027	1.567TyrVal: 1.567 ± 0.024	0.341TyrTrp: 0.341 ± 0.011	1.547TyrTyr: 1.547 ± 0.026	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.002XaaLys: 0.002 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6456 proteins (2976403 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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