Amino acid dipepetide frequency for Rhizobium sp. BK376

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.174AlaAla: 15.174 ± 0.116
0.951AlaCys: 0.951 ± 0.021
6.597AlaAsp: 6.597 ± 0.056
6.851AlaGlu: 6.851 ± 0.063
4.478AlaPhe: 4.478 ± 0.046
9.369AlaGly: 9.369 ± 0.075
2.071AlaHis: 2.071 ± 0.033
7.127AlaIle: 7.127 ± 0.065
4.406AlaLys: 4.406 ± 0.054
12.028AlaLeu: 12.028 ± 0.097
3.451AlaMet: 3.451 ± 0.04
3.137AlaAsn: 3.137 ± 0.041
4.609AlaPro: 4.609 ± 0.07
3.48AlaGln: 3.48 ± 0.053
7.094AlaArg: 7.094 ± 0.06
6.836AlaSer: 6.836 ± 0.061
5.72AlaThr: 5.72 ± 0.056
8.339AlaVal: 8.339 ± 0.068
1.32AlaTrp: 1.32 ± 0.026
2.519AlaTyr: 2.519 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
0.85CysAla: 0.85 ± 0.021
0.111CysCys: 0.111 ± 0.007
0.48CysAsp: 0.48 ± 0.014
0.429CysGlu: 0.429 ± 0.014
0.344CysPhe: 0.344 ± 0.012
0.869CysGly: 0.869 ± 0.02
0.243CysHis: 0.243 ± 0.011
0.457CysIle: 0.457 ± 0.015
0.206CysLys: 0.206 ± 0.01
0.824CysLeu: 0.824 ± 0.018
0.171CysMet: 0.171 ± 0.009
0.222CysAsn: 0.222 ± 0.01
0.373CysPro: 0.373 ± 0.013
0.219CysGln: 0.219 ± 0.01
0.575CysArg: 0.575 ± 0.019
0.521CysSer: 0.521 ± 0.017
0.377CysThr: 0.377 ± 0.013
0.571CysVal: 0.571 ± 0.016
0.11CysTrp: 0.11 ± 0.007
0.203CysTyr: 0.203 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
6.376AspAla: 6.376 ± 0.07
0.485AspCys: 0.485 ± 0.015
3.281AspAsp: 3.281 ± 0.043
3.467AspGlu: 3.467 ± 0.046
2.403AspPhe: 2.403 ± 0.037
4.983AspGly: 4.983 ± 0.054
1.33AspHis: 1.33 ± 0.026
3.601AspIle: 3.601 ± 0.041
1.983AspLys: 1.983 ± 0.027
5.897AspLeu: 5.897 ± 0.052
1.468AspMet: 1.468 ± 0.028
1.571AspAsn: 1.571 ± 0.027
3.218AspPro: 3.218 ± 0.043
1.864AspGln: 1.864 ± 0.031
4.153AspArg: 4.153 ± 0.047
2.353AspSer: 2.353 ± 0.033
2.561AspThr: 2.561 ± 0.04
4.205AspVal: 4.205 ± 0.046
0.911AspTrp: 0.911 ± 0.021
1.529AspTyr: 1.529 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
6.831GluAla: 6.831 ± 0.06
0.33GluCys: 0.33 ± 0.012
2.832GluAsp: 2.832 ± 0.042
3.41GluGlu: 3.41 ± 0.049
1.873GluPhe: 1.873 ± 0.03
3.995GluGly: 3.995 ± 0.048
1.174GluHis: 1.174 ± 0.024
3.761GluIle: 3.761 ± 0.046
2.661GluLys: 2.661 ± 0.038
5.262GluLeu: 5.262 ± 0.053
1.547GluMet: 1.547 ± 0.027
1.762GluAsn: 1.762 ± 0.028
2.488GluPro: 2.488 ± 0.036
2.021GluGln: 2.021 ± 0.034
4.604GluArg: 4.604 ± 0.052
2.373GluSer: 2.373 ± 0.031
3.464GluThr: 3.464 ± 0.045
3.535GluVal: 3.535 ± 0.045
0.717GluTrp: 0.717 ± 0.017
1.066GluTyr: 1.066 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
4.519PheAla: 4.519 ± 0.046
0.398PheCys: 0.398 ± 0.013
2.784PheAsp: 2.784 ± 0.033
2.186PheGlu: 2.186 ± 0.032
1.603PhePhe: 1.603 ± 0.03
3.746PheGly: 3.746 ± 0.047
0.845PheHis: 0.845 ± 0.021
2.023PheIle: 2.023 ± 0.036
1.136PheLys: 1.136 ± 0.03
3.629PheLeu: 3.629 ± 0.043
0.855PheMet: 0.855 ± 0.019
1.202PheAsn: 1.202 ± 0.025
1.659PhePro: 1.659 ± 0.032
1.131PheGln: 1.131 ± 0.024
2.325PheArg: 2.325 ± 0.031
2.64PheSer: 2.64 ± 0.039
1.971PheThr: 1.971 ± 0.03
2.992PheVal: 2.992 ± 0.036
0.574PheTrp: 0.574 ± 0.018
1.038PheTyr: 1.038 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
8.02GlyAla: 8.02 ± 0.074
0.792GlyCys: 0.792 ± 0.02
4.281GlyAsp: 4.281 ± 0.054
4.426GlyGlu: 4.426 ± 0.051
3.659GlyPhe: 3.659 ± 0.047
6.648GlyGly: 6.648 ± 0.073
1.861GlyHis: 1.861 ± 0.029
5.116GlyIle: 5.116 ± 0.05
3.747GlyLys: 3.747 ± 0.048
8.415GlyLeu: 8.415 ± 0.074
2.287GlyMet: 2.287 ± 0.031
2.48GlyAsn: 2.48 ± 0.047
3.163GlyPro: 3.163 ± 0.034
2.711GlyGln: 2.711 ± 0.043
5.457GlyArg: 5.457 ± 0.057
4.946GlySer: 4.946 ± 0.053
4.487GlyThr: 4.487 ± 0.051
5.685GlyVal: 5.685 ± 0.054
1.265GlyTrp: 1.265 ± 0.027
2.417GlyTyr: 2.417 ± 0.033
0.0GlyXaa: 0.0 ± 0.0
His
2.136HisAla: 2.136 ± 0.033
0.21HisCys: 0.21 ± 0.01
1.274HisAsp: 1.274 ± 0.024
1.04HisGlu: 1.04 ± 0.022
0.955HisPhe: 0.955 ± 0.018
1.863HisGly: 1.863 ± 0.035
0.594HisHis: 0.594 ± 0.02
1.071HisIle: 1.071 ± 0.026
0.531HisLys: 0.531 ± 0.015
2.165HisLeu: 2.165 ± 0.035
0.583HisMet: 0.583 ± 0.017
0.518HisAsn: 0.518 ± 0.015
1.306HisPro: 1.306 ± 0.028
0.642HisGln: 0.642 ± 0.018
1.387HisArg: 1.387 ± 0.026
1.115HisSer: 1.115 ± 0.025
0.826HisThr: 0.826 ± 0.018
1.555HisVal: 1.555 ± 0.026
0.349HisTrp: 0.349 ± 0.013
0.58HisTyr: 0.58 ± 0.016
0.0HisXaa: 0.0 ± 0.0
Ile
7.968IleAla: 7.968 ± 0.067
0.612IleCys: 0.612 ± 0.017
3.946IleAsp: 3.946 ± 0.038
3.695IleGlu: 3.695 ± 0.045
2.172IlePhe: 2.172 ± 0.035
5.395IleGly: 5.395 ± 0.054
1.041IleHis: 1.041 ± 0.024
2.967IleIle: 2.967 ± 0.042
1.831IleLys: 1.831 ± 0.034
5.185IleLeu: 5.185 ± 0.053
1.164IleMet: 1.164 ± 0.024
1.727IleAsn: 1.727 ± 0.032
2.599IlePro: 2.599 ± 0.036
1.388IleGln: 1.388 ± 0.029
3.505IleArg: 3.505 ± 0.044
3.818IleSer: 3.818 ± 0.046
2.901IleThr: 2.901 ± 0.043
4.762IleVal: 4.762 ± 0.053
0.662IleTrp: 0.662 ± 0.021
1.303IleTyr: 1.303 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.741LysAla: 4.741 ± 0.061
0.184LysCys: 0.184 ± 0.008
2.053LysAsp: 2.053 ± 0.031
1.876LysGlu: 1.876 ± 0.035
1.062LysPhe: 1.062 ± 0.019
2.881LysGly: 2.881 ± 0.041
0.698LysHis: 0.698 ± 0.018
2.172LysIle: 2.172 ± 0.033
1.576LysLys: 1.576 ± 0.034
3.724LysLeu: 3.724 ± 0.05
0.957LysMet: 0.957 ± 0.019
1.081LysAsn: 1.081 ± 0.024
2.318LysPro: 2.318 ± 0.034
1.268LysGln: 1.268 ± 0.025
2.531LysArg: 2.531 ± 0.036
2.359LysSer: 2.359 ± 0.031
2.339LysThr: 2.339 ± 0.035
2.729LysVal: 2.729 ± 0.036
0.427LysTrp: 0.427 ± 0.014
0.711LysTyr: 0.711 ± 0.019
0.0LysXaa: 0.0 ± 0.0
Leu
12.076LeuAla: 12.076 ± 0.08
0.882LeuCys: 0.882 ± 0.019
5.882LeuAsp: 5.882 ± 0.052
4.982LeuGlu: 4.982 ± 0.06
3.795LeuPhe: 3.795 ± 0.051
7.853LeuGly: 7.853 ± 0.067
1.829LeuHis: 1.829 ± 0.032
5.394LeuIle: 5.394 ± 0.056
4.083LeuLys: 4.083 ± 0.041
9.505LeuLeu: 9.505 ± 0.096
2.417LeuMet: 2.417 ± 0.035
2.829LeuAsn: 2.829 ± 0.034
5.241LeuPro: 5.241 ± 0.054
3.012LeuGln: 3.012 ± 0.039
6.224LeuArg: 6.224 ± 0.061
7.34LeuSer: 7.34 ± 0.086
5.61LeuThr: 5.61 ± 0.064
7.273LeuVal: 7.273 ± 0.061
1.079LeuTrp: 1.079 ± 0.022
2.144LeuTyr: 2.144 ± 0.031
0.0LeuXaa: 0.0 ± 0.0
Met
3.001MetAla: 3.001 ± 0.036
0.151MetCys: 0.151 ± 0.009
1.23MetAsp: 1.23 ± 0.02
1.209MetGlu: 1.209 ± 0.027
0.759MetPhe: 0.759 ± 0.017
1.727MetGly: 1.727 ± 0.028
0.491MetHis: 0.491 ± 0.015
1.586MetIle: 1.586 ± 0.026
1.143MetLys: 1.143 ± 0.023
2.583MetLeu: 2.583 ± 0.035
0.721MetMet: 0.721 ± 0.021
0.917MetAsn: 0.917 ± 0.019
1.512MetPro: 1.512 ± 0.027
0.861MetGln: 0.861 ± 0.02
1.793MetArg: 1.793 ± 0.027
1.882MetSer: 1.882 ± 0.03
2.0MetThr: 2.0 ± 0.031
1.738MetVal: 1.738 ± 0.029
0.193MetTrp: 0.193 ± 0.01
0.341MetTyr: 0.341 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.426AsnAla: 3.426 ± 0.041
0.247AsnCys: 0.247 ± 0.012
1.598AsnAsp: 1.598 ± 0.027
1.443AsnGlu: 1.443 ± 0.027
1.166AsnPhe: 1.166 ± 0.025
2.797AsnGly: 2.797 ± 0.042
0.594AsnHis: 0.594 ± 0.018
1.642AsnIle: 1.642 ± 0.032
0.891AsnLys: 0.891 ± 0.019
2.931AsnLeu: 2.931 ± 0.037
0.721AsnMet: 0.721 ± 0.016
0.863AsnAsn: 0.863 ± 0.023
1.926AsnPro: 1.926 ± 0.028
0.897AsnGln: 0.897 ± 0.022
1.923AsnArg: 1.923 ± 0.029
1.624AsnSer: 1.624 ± 0.026
1.344AsnThr: 1.344 ± 0.025
2.135AsnVal: 2.135 ± 0.04
0.467AsnTrp: 0.467 ± 0.014
0.766AsnTyr: 0.766 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
5.355ProAla: 5.355 ± 0.067
0.276ProCys: 0.276 ± 0.01
3.481ProAsp: 3.481 ± 0.045
3.344ProGlu: 3.344 ± 0.041
1.99ProPhe: 1.99 ± 0.03
3.896ProGly: 3.896 ± 0.046
1.028ProHis: 1.028 ± 0.026
2.556ProIle: 2.556 ± 0.034
1.826ProLys: 1.826 ± 0.031
4.59ProLeu: 4.59 ± 0.046
1.118ProMet: 1.118 ± 0.023
1.467ProAsn: 1.467 ± 0.028
2.236ProPro: 2.236 ± 0.045
1.685ProGln: 1.685 ± 0.036
2.544ProArg: 2.544 ± 0.037
3.007ProSer: 3.007 ± 0.035
2.504ProThr: 2.504 ± 0.037
3.959ProVal: 3.959 ± 0.046
0.651ProTrp: 0.651 ± 0.018
1.267ProTyr: 1.267 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.853GlnAla: 3.853 ± 0.044
0.192GlnCys: 0.192 ± 0.01
1.48GlnAsp: 1.48 ± 0.029
1.585GlnGlu: 1.585 ± 0.027
1.16GlnPhe: 1.16 ± 0.025
2.23GlnGly: 2.23 ± 0.034
0.652GlnHis: 0.652 ± 0.016
2.037GlnIle: 2.037 ± 0.038
1.325GlnLys: 1.325 ± 0.024
2.967GlnLeu: 2.967 ± 0.038
0.956GlnMet: 0.956 ± 0.02
0.958GlnAsn: 0.958 ± 0.02
1.749GlnPro: 1.749 ± 0.035
1.43GlnGln: 1.43 ± 0.032
2.303GlnArg: 2.303 ± 0.036
2.02GlnSer: 2.02 ± 0.033
1.807GlnThr: 1.807 ± 0.033
2.113GlnVal: 2.113 ± 0.038
0.403GlnTrp: 0.403 ± 0.012
0.669GlnTyr: 0.669 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
6.429ArgAla: 6.429 ± 0.064
0.466ArgCys: 0.466 ± 0.014
3.737ArgAsp: 3.737 ± 0.049
3.831ArgGlu: 3.831 ± 0.05
2.745ArgPhe: 2.745 ± 0.04
4.3ArgGly: 4.3 ± 0.046
1.665ArgHis: 1.665 ± 0.032
4.059ArgIle: 4.059 ± 0.044
2.533ArgLys: 2.533 ± 0.037
7.191ArgLeu: 7.191 ± 0.068
1.833ArgMet: 1.833 ± 0.031
2.063ArgAsn: 2.063 ± 0.03
3.096ArgPro: 3.096 ± 0.037
2.57ArgGln: 2.57 ± 0.037
5.098ArgArg: 5.098 ± 0.056
3.971ArgSer: 3.971 ± 0.045
3.205ArgThr: 3.205 ± 0.037
4.139ArgVal: 4.139 ± 0.043
0.895ArgTrp: 0.895 ± 0.022
1.731ArgTyr: 1.731 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.569SerAla: 6.569 ± 0.066
0.445SerCys: 0.445 ± 0.013
3.522SerAsp: 3.522 ± 0.04
3.141SerGlu: 3.141 ± 0.043
2.565SerPhe: 2.565 ± 0.036
5.984SerGly: 5.984 ± 0.068
1.261SerHis: 1.261 ± 0.027
3.52SerIle: 3.52 ± 0.046
2.124SerLys: 2.124 ± 0.036
5.972SerLeu: 5.972 ± 0.06
1.552SerMet: 1.552 ± 0.028
1.801SerAsn: 1.801 ± 0.031
2.947SerPro: 2.947 ± 0.04
1.905SerGln: 1.905 ± 0.033
3.831SerArg: 3.831 ± 0.047
3.833SerSer: 3.833 ± 0.057
3.181SerThr: 3.181 ± 0.063
4.493SerVal: 4.493 ± 0.045
0.821SerTrp: 0.821 ± 0.016
1.477SerTyr: 1.477 ± 0.028
0.0SerXaa: 0.0 ± 0.0
Thr
6.023ThrAla: 6.023 ± 0.055
0.401ThrCys: 0.401 ± 0.014
2.991ThrAsp: 2.991 ± 0.042
2.705ThrGlu: 2.705 ± 0.035
2.096ThrPhe: 2.096 ± 0.033
4.849ThrGly: 4.849 ± 0.051
1.075ThrHis: 1.075 ± 0.023
3.375ThrIle: 3.375 ± 0.043
1.824ThrLys: 1.824 ± 0.029
5.579ThrLeu: 5.579 ± 0.06
1.338ThrMet: 1.338 ± 0.026
1.49ThrAsn: 1.49 ± 0.028
2.998ThrPro: 2.998 ± 0.037
1.468ThrGln: 1.468 ± 0.031
2.929ThrArg: 2.929 ± 0.041
3.186ThrSer: 3.186 ± 0.048
3.005ThrThr: 3.005 ± 0.052
4.35ThrVal: 4.35 ± 0.046
0.659ThrTrp: 0.659 ± 0.017
1.279ThrTyr: 1.279 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
8.646ValAla: 8.646 ± 0.072
0.623ValCys: 0.623 ± 0.018
4.087ValAsp: 4.087 ± 0.049
4.283ValGlu: 4.283 ± 0.048
2.882ValPhe: 2.882 ± 0.037
5.426ValGly: 5.426 ± 0.049
1.389ValHis: 1.389 ± 0.024
4.361ValIle: 4.361 ± 0.046
2.574ValLys: 2.574 ± 0.037
7.063ValLeu: 7.063 ± 0.065
1.85ValMet: 1.85 ± 0.029
2.106ValAsn: 2.106 ± 0.038
3.53ValPro: 3.53 ± 0.04
2.009ValGln: 2.009 ± 0.027
4.571ValArg: 4.571 ± 0.054
4.862ValSer: 4.862 ± 0.053
4.429ValThr: 4.429 ± 0.043
5.847ValVal: 5.847 ± 0.059
0.836ValTrp: 0.836 ± 0.019
1.491ValTyr: 1.491 ± 0.023
0.0ValXaa: 0.0 ± 0.0
Trp
1.128TrpAla: 1.128 ± 0.023
0.139TrpCys: 0.139 ± 0.009
0.632TrpAsp: 0.632 ± 0.017
0.548TrpGlu: 0.548 ± 0.016
0.514TrpPhe: 0.514 ± 0.016
0.844TrpGly: 0.844 ± 0.021
0.349TrpHis: 0.349 ± 0.014
0.7TrpIle: 0.7 ± 0.018
0.543TrpLys: 0.543 ± 0.017
1.585TrpLeu: 1.585 ± 0.031
0.363TrpMet: 0.363 ± 0.012
0.517TrpAsn: 0.517 ± 0.016
0.652TrpPro: 0.652 ± 0.018
0.57TrpGln: 0.57 ± 0.017
0.969TrpArg: 0.969 ± 0.02
0.863TrpSer: 0.863 ± 0.02
0.756TrpThr: 0.756 ± 0.02
0.755TrpVal: 0.755 ± 0.018
0.211TrpTrp: 0.211 ± 0.011
0.308TrpTyr: 0.308 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.435TyrAla: 2.435 ± 0.031
0.259TyrCys: 0.259 ± 0.011
1.474TyrAsp: 1.474 ± 0.03
1.258TyrGlu: 1.258 ± 0.026
1.038TyrPhe: 1.038 ± 0.023
2.208TyrGly: 2.208 ± 0.036
0.506TyrHis: 0.506 ± 0.015
1.115TyrIle: 1.115 ± 0.02
0.743TyrLys: 0.743 ± 0.018
2.354TyrLeu: 2.354 ± 0.038
0.488TyrMet: 0.488 ± 0.014
0.691TyrAsn: 0.691 ± 0.017
1.159TyrPro: 1.159 ± 0.021
0.759TyrGln: 0.759 ± 0.017
1.742TyrArg: 1.742 ± 0.031
1.392TyrSer: 1.392 ± 0.027
1.135TyrThr: 1.135 ± 0.025
1.689TyrVal: 1.689 ± 0.032
0.396TyrTrp: 0.396 ± 0.014
0.668TyrTyr: 0.668 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7471 proteins (2212000 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski