methanotrophic bacterial endosymbiont of Bathymodiolus sp.

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; unclassified Gammaproteobacteria

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5290 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X7MER8|A0A1X7MER8_9GAMM RNA pyrophosphohydrolase OS=methanotrophic bacterial endosymbiont of Bathymodiolus sp. OX=280772 GN=rppH PE=3 SV=1
TT1 pKa = 6.6VVNGPDD7 pKa = 4.51SGVCWDD13 pKa = 3.66CHH15 pKa = 6.44QYY17 pKa = 10.49MNDD20 pKa = 2.84VGFALDD26 pKa = 4.39SFDD29 pKa = 3.54TQGKK33 pKa = 8.92YY34 pKa = 7.54RR35 pKa = 11.84TTEE38 pKa = 3.94TVQGIGSQTGIEE50 pKa = 4.21ADD52 pKa = 3.72FDD54 pKa = 3.96IQTEE58 pKa = 4.98GYY60 pKa = 9.73FISDD64 pKa = 3.24NADD67 pKa = 3.04DD68 pKa = 4.65VFFIDD73 pKa = 6.21LRR75 pKa = 11.84DD76 pKa = 3.41LSQIIGSSDD85 pKa = 3.06KK86 pKa = 10.2NTEE89 pKa = 4.4CISTRR94 pKa = 11.84YY95 pKa = 9.04LQFVRR100 pKa = 11.84GYY102 pKa = 9.53KK103 pKa = 10.48GEE105 pKa = 4.45SSDD108 pKa = 3.58STYY111 pKa = 10.48LTDD114 pKa = 3.58VYY116 pKa = 11.04KK117 pKa = 11.05

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X7MLY8|A0A1X7MLY8_9GAMM Uncharacterized protein OS=methanotrophic bacterial endosymbiont of Bathymodiolus sp. OX=280772 GN=BMETH_910_0 PE=4 SV=1
MM1 pKa = 7.23NGSRR5 pKa = 11.84SVRR8 pKa = 11.84SQQARR13 pKa = 11.84QSRR16 pKa = 11.84SSINSTWPSPRR27 pKa = 11.84RR28 pKa = 11.84SNKK31 pKa = 8.62RR32 pKa = 11.84QKK34 pKa = 9.54TGLASRR40 pKa = 11.84RR41 pKa = 3.76

Molecular weight: 4.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5290

0

5290

782836

19

1677

148.0

16.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.453 ± 0.049

1.233 ± 0.019

5.291 ± 0.032

5.85 ± 0.038

4.197 ± 0.03

6.465 ± 0.048

2.302 ± 0.023

7.067 ± 0.037

5.741 ± 0.036

10.386 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.661 ± 0.023

4.331 ± 0.026

4.023 ± 0.025

4.615 ± 0.038

4.672 ± 0.034

6.591 ± 0.035

5.302 ± 0.03

6.443 ± 0.04

1.248 ± 0.016

3.128 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski