Amino acid dipepetide frequency for methanotrophic bacterial endosymbiont of Bathymodiolus sp.

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.381AlaAla: 7.381 ± 0.125
1.095AlaCys: 1.095 ± 0.039
4.634AlaAsp: 4.634 ± 0.07
5.661AlaGlu: 5.661 ± 0.096
3.2AlaPhe: 3.2 ± 0.072
5.865AlaGly: 5.865 ± 0.092
1.744AlaHis: 1.744 ± 0.046
6.166AlaIle: 6.166 ± 0.091
4.876AlaLys: 4.876 ± 0.084
9.168AlaLeu: 9.168 ± 0.115
2.539AlaMet: 2.539 ± 0.052
3.377AlaAsn: 3.377 ± 0.069
2.726AlaPro: 2.726 ± 0.072
3.731AlaGln: 3.731 ± 0.07
3.965AlaArg: 3.965 ± 0.072
4.947AlaSer: 4.947 ± 0.091
4.252AlaThr: 4.252 ± 0.085
5.627AlaVal: 5.627 ± 0.091
1.079AlaTrp: 1.079 ± 0.036
2.499AlaTyr: 2.499 ± 0.061
0.0AlaXaa: 0.0 ± 0.0
Cys
0.963CysAla: 0.963 ± 0.036
0.254CysCys: 0.254 ± 0.021
0.602CysAsp: 0.602 ± 0.028
0.566CysGlu: 0.566 ± 0.028
0.723CysPhe: 0.723 ± 0.031
0.812CysGly: 0.812 ± 0.03
0.442CysHis: 0.442 ± 0.029
0.789CysIle: 0.789 ± 0.036
0.538CysLys: 0.538 ± 0.03
1.331CysLeu: 1.331 ± 0.036
0.293CysMet: 0.293 ± 0.018
0.446CysAsn: 0.446 ± 0.026
0.57CysPro: 0.57 ± 0.028
0.507CysGln: 0.507 ± 0.026
0.577CysArg: 0.577 ± 0.026
0.958CysSer: 0.958 ± 0.039
0.659CysThr: 0.659 ± 0.033
0.71CysVal: 0.71 ± 0.028
0.161CysTrp: 0.161 ± 0.014
0.424CysTyr: 0.424 ± 0.021
0.001CysXaa: 0.001 ± 0.001
Asp
4.272AspAla: 4.272 ± 0.084
0.632AspCys: 0.632 ± 0.03
2.828AspAsp: 2.828 ± 0.069
3.471AspGlu: 3.471 ± 0.071
2.623AspPhe: 2.623 ± 0.055
3.241AspGly: 3.241 ± 0.064
0.991AspHis: 0.991 ± 0.035
4.111AspIle: 4.111 ± 0.077
3.458AspLys: 3.458 ± 0.067
5.249AspLeu: 5.249 ± 0.086
1.346AspMet: 1.346 ± 0.041
2.368AspAsn: 2.368 ± 0.06
1.951AspPro: 1.951 ± 0.06
2.05AspGln: 2.05 ± 0.054
2.285AspArg: 2.285 ± 0.051
3.259AspSer: 3.259 ± 0.073
2.629AspThr: 2.629 ± 0.059
3.243AspVal: 3.243 ± 0.063
0.851AspTrp: 0.851 ± 0.031
2.057AspTyr: 2.057 ± 0.056
0.0AspXaa: 0.0 ± 0.0
Glu
4.53GluAla: 4.53 ± 0.085
0.535GluCys: 0.535 ± 0.027
2.772GluAsp: 2.772 ± 0.06
3.317GluGlu: 3.317 ± 0.077
2.267GluPhe: 2.267 ± 0.059
3.225GluGly: 3.225 ± 0.073
1.493GluHis: 1.493 ± 0.045
4.398GluIle: 4.398 ± 0.082
3.941GluLys: 3.941 ± 0.076
6.295GluLeu: 6.295 ± 0.084
1.585GluMet: 1.585 ± 0.045
2.817GluAsn: 2.817 ± 0.063
1.869GluPro: 1.869 ± 0.048
3.818GluGln: 3.818 ± 0.089
2.938GluArg: 2.938 ± 0.063
3.468GluSer: 3.468 ± 0.06
3.024GluThr: 3.024 ± 0.07
3.786GluVal: 3.786 ± 0.07
0.621GluTrp: 0.621 ± 0.031
1.8GluTyr: 1.8 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
3.236PheAla: 3.236 ± 0.062
0.643PheCys: 0.643 ± 0.027
2.389PheAsp: 2.389 ± 0.047
2.05PheGlu: 2.05 ± 0.051
2.035PhePhe: 2.035 ± 0.06
2.593PheGly: 2.593 ± 0.06
0.83PheHis: 0.83 ± 0.034
3.242PheIle: 3.242 ± 0.061
2.303PheLys: 2.303 ± 0.057
4.141PheLeu: 4.141 ± 0.08
1.122PheMet: 1.122 ± 0.045
2.095PheAsn: 2.095 ± 0.055
1.779PhePro: 1.779 ± 0.05
1.404PheGln: 1.404 ± 0.046
1.659PheArg: 1.659 ± 0.04
3.55PheSer: 3.55 ± 0.064
2.312PheThr: 2.312 ± 0.061
2.577PheVal: 2.577 ± 0.058
0.561PheTrp: 0.561 ± 0.025
1.454PheTyr: 1.454 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
4.789GlyAla: 4.789 ± 0.081
1.012GlyCys: 1.012 ± 0.043
3.261GlyAsp: 3.261 ± 0.067
3.794GlyGlu: 3.794 ± 0.076
3.141GlyPhe: 3.141 ± 0.077
4.516GlyGly: 4.516 ± 0.095
1.461GlyHis: 1.461 ± 0.044
4.717GlyIle: 4.717 ± 0.081
4.15GlyLys: 4.15 ± 0.074
6.654GlyLeu: 6.654 ± 0.099
2.004GlyMet: 2.004 ± 0.054
2.686GlyAsn: 2.686 ± 0.061
1.423GlyPro: 1.423 ± 0.041
2.546GlyGln: 2.546 ± 0.057
3.061GlyArg: 3.061 ± 0.064
4.053GlySer: 4.053 ± 0.076
3.21GlyThr: 3.21 ± 0.071
4.898GlyVal: 4.898 ± 0.088
0.94GlyTrp: 0.94 ± 0.037
2.338GlyTyr: 2.338 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
1.911HisAla: 1.911 ± 0.049
0.414HisCys: 0.414 ± 0.025
1.086HisAsp: 1.086 ± 0.039
1.134HisGlu: 1.134 ± 0.042
1.12HisPhe: 1.12 ± 0.045
1.562HisGly: 1.562 ± 0.045
0.63HisHis: 0.63 ± 0.03
1.578HisIle: 1.578 ± 0.041
1.252HisLys: 1.252 ± 0.038
2.347HisLeu: 2.347 ± 0.059
0.582HisMet: 0.582 ± 0.029
0.995HisAsn: 0.995 ± 0.034
1.238HisPro: 1.238 ± 0.046
1.078HisGln: 1.078 ± 0.038
1.082HisArg: 1.082 ± 0.037
1.447HisSer: 1.447 ± 0.041
1.109HisThr: 1.109 ± 0.039
1.217HisVal: 1.217 ± 0.039
0.34HisTrp: 0.34 ± 0.022
0.895HisTyr: 0.895 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
6.695IleAla: 6.695 ± 0.09
0.837IleCys: 0.837 ± 0.032
4.45IleAsp: 4.45 ± 0.082
4.551IleGlu: 4.551 ± 0.091
2.626IlePhe: 2.626 ± 0.067
4.387IleGly: 4.387 ± 0.079
1.472IleHis: 1.472 ± 0.047
4.983IleIle: 4.983 ± 0.095
4.309IleLys: 4.309 ± 0.086
6.176IleLeu: 6.176 ± 0.085
1.579IleMet: 1.579 ± 0.043
3.535IleAsn: 3.535 ± 0.063
2.983IlePro: 2.983 ± 0.061
2.778IleGln: 2.778 ± 0.063
3.156IleArg: 3.156 ± 0.065
5.204IleSer: 5.204 ± 0.096
3.911IleThr: 3.911 ± 0.067
4.244IleVal: 4.244 ± 0.074
0.682IleTrp: 0.682 ± 0.03
2.109IleTyr: 2.109 ± 0.053
0.0IleXaa: 0.0 ± 0.0
Lys
4.763LysAla: 4.763 ± 0.083
0.442LysCys: 0.442 ± 0.026
2.854LysAsp: 2.854 ± 0.066
3.335LysGlu: 3.335 ± 0.065
1.723LysPhe: 1.723 ± 0.053
3.406LysGly: 3.406 ± 0.072
1.433LysHis: 1.433 ± 0.042
4.177LysIle: 4.177 ± 0.07
3.925LysLys: 3.925 ± 0.087
5.58LysLeu: 5.58 ± 0.085
1.58LysMet: 1.58 ± 0.052
2.845LysAsn: 2.845 ± 0.067
2.451LysPro: 2.451 ± 0.053
3.353LysGln: 3.353 ± 0.069
2.804LysArg: 2.804 ± 0.072
3.495LysSer: 3.495 ± 0.073
3.453LysThr: 3.453 ± 0.073
3.609LysVal: 3.609 ± 0.072
0.538LysTrp: 0.538 ± 0.026
1.645LysTyr: 1.645 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
9.627LeuAla: 9.627 ± 0.13
1.244LeuCys: 1.244 ± 0.037
5.446LeuAsp: 5.446 ± 0.085
5.803LeuGlu: 5.803 ± 0.08
4.383LeuPhe: 4.383 ± 0.086
6.393LeuGly: 6.393 ± 0.091
2.202LeuHis: 2.202 ± 0.045
7.161LeuIle: 7.161 ± 0.097
5.803LeuLys: 5.803 ± 0.089
10.944LeuLeu: 10.944 ± 0.162
2.782LeuMet: 2.782 ± 0.056
4.688LeuAsn: 4.688 ± 0.086
4.763LeuPro: 4.763 ± 0.083
4.583LeuGln: 4.583 ± 0.092
4.688LeuArg: 4.688 ± 0.074
7.602LeuSer: 7.602 ± 0.117
5.646LeuThr: 5.646 ± 0.091
6.101LeuVal: 6.101 ± 0.079
1.188LeuTrp: 1.188 ± 0.039
2.81LeuTyr: 2.81 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
2.5MetAla: 2.5 ± 0.064
0.247MetCys: 0.247 ± 0.017
1.308MetAsp: 1.308 ± 0.04
1.233MetGlu: 1.233 ± 0.04
0.815MetPhe: 0.815 ± 0.034
1.829MetGly: 1.829 ± 0.049
0.645MetHis: 0.645 ± 0.027
1.765MetIle: 1.765 ± 0.045
1.401MetLys: 1.401 ± 0.034
2.849MetLeu: 2.849 ± 0.061
0.724MetMet: 0.724 ± 0.035
1.178MetAsn: 1.178 ± 0.038
1.464MetPro: 1.464 ± 0.045
1.468MetGln: 1.468 ± 0.043
1.306MetArg: 1.306 ± 0.04
1.866MetSer: 1.866 ± 0.055
1.644MetThr: 1.644 ± 0.045
1.636MetVal: 1.636 ± 0.051
0.178MetTrp: 0.178 ± 0.016
0.558MetTyr: 0.558 ± 0.026
0.0MetXaa: 0.0 ± 0.0
Asn
3.417AsnAla: 3.417 ± 0.066
0.515AsnCys: 0.515 ± 0.026
2.112AsnAsp: 2.112 ± 0.056
2.306AsnGlu: 2.306 ± 0.053
1.631AsnPhe: 1.631 ± 0.045
2.702AsnGly: 2.702 ± 0.065
0.959AsnHis: 0.959 ± 0.032
3.444AsnIle: 3.444 ± 0.064
2.76AsnLys: 2.76 ± 0.051
3.965AsnLeu: 3.965 ± 0.077
1.09AsnMet: 1.09 ± 0.04
2.191AsnAsn: 2.191 ± 0.056
2.261AsnPro: 2.261 ± 0.062
2.043AsnGln: 2.043 ± 0.05
2.026AsnArg: 2.026 ± 0.057
2.934AsnSer: 2.934 ± 0.068
2.524AsnThr: 2.524 ± 0.063
2.364AsnVal: 2.364 ± 0.059
0.6AsnTrp: 0.6 ± 0.027
1.466AsnTyr: 1.466 ± 0.045
0.0AsnXaa: 0.0 ± 0.0
Pro
3.441ProAla: 3.441 ± 0.064
0.506ProCys: 0.506 ± 0.028
2.649ProAsp: 2.649 ± 0.062
3.342ProGlu: 3.342 ± 0.063
1.741ProPhe: 1.741 ± 0.044
2.598ProGly: 2.598 ± 0.062
0.959ProHis: 0.959 ± 0.04
2.52ProIle: 2.52 ± 0.062
2.029ProLys: 2.029 ± 0.052
3.974ProLeu: 3.974 ± 0.076
0.967ProMet: 0.967 ± 0.032
1.481ProAsn: 1.481 ± 0.047
1.438ProPro: 1.438 ± 0.054
1.326ProGln: 1.326 ± 0.043
1.68ProArg: 1.68 ± 0.053
2.308ProSer: 2.308 ± 0.06
2.062ProThr: 2.062 ± 0.052
3.33ProVal: 3.33 ± 0.072
0.618ProTrp: 0.618 ± 0.028
1.26ProTyr: 1.26 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
4.528GlnAla: 4.528 ± 0.078
0.497GlnCys: 0.497 ± 0.022
2.193GlnAsp: 2.193 ± 0.058
2.571GlnGlu: 2.571 ± 0.064
1.709GlnPhe: 1.709 ± 0.045
2.951GlnGly: 2.951 ± 0.063
1.251GlnHis: 1.251 ± 0.041
2.762GlnIle: 2.762 ± 0.066
2.436GlnLys: 2.436 ± 0.064
5.108GlnLeu: 5.108 ± 0.09
1.148GlnMet: 1.148 ± 0.032
1.583GlnAsn: 1.583 ± 0.048
1.643GlnPro: 1.643 ± 0.052
3.431GlnGln: 3.431 ± 0.089
2.381GlnArg: 2.381 ± 0.055
2.746GlnSer: 2.746 ± 0.055
2.256GlnThr: 2.256 ± 0.057
3.048GlnVal: 3.048 ± 0.069
0.58GlnTrp: 0.58 ± 0.026
1.326GlnTyr: 1.326 ± 0.038
0.0GlnXaa: 0.0 ± 0.0
Arg
3.485ArgAla: 3.485 ± 0.077
0.584ArgCys: 0.584 ± 0.028
2.293ArgAsp: 2.293 ± 0.055
2.783ArgGlu: 2.783 ± 0.06
2.109ArgPhe: 2.109 ± 0.054
2.69ArgGly: 2.69 ± 0.058
1.234ArgHis: 1.234 ± 0.039
3.361ArgIle: 3.361 ± 0.071
2.666ArgLys: 2.666 ± 0.054
5.249ArgLeu: 5.249 ± 0.089
1.406ArgMet: 1.406 ± 0.041
1.972ArgAsn: 1.972 ± 0.05
1.751ArgPro: 1.751 ± 0.053
2.178ArgGln: 2.178 ± 0.048
2.398ArgArg: 2.398 ± 0.06
2.838ArgSer: 2.838 ± 0.062
2.187ArgThr: 2.187 ± 0.052
3.053ArgVal: 3.053 ± 0.061
0.674ArgTrp: 0.674 ± 0.032
1.81ArgTyr: 1.81 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
5.526SerAla: 5.526 ± 0.089
0.901SerCys: 0.901 ± 0.036
3.315SerAsp: 3.315 ± 0.064
3.604SerGlu: 3.604 ± 0.069
2.849SerPhe: 2.849 ± 0.064
4.904SerGly: 4.904 ± 0.082
1.521SerHis: 1.521 ± 0.045
4.577SerIle: 4.577 ± 0.086
3.308SerLys: 3.308 ± 0.064
7.068SerLeu: 7.068 ± 0.095
1.776SerMet: 1.776 ± 0.051
2.757SerAsn: 2.757 ± 0.06
2.718SerPro: 2.718 ± 0.058
2.501SerGln: 2.501 ± 0.059
3.11SerArg: 3.11 ± 0.066
4.748SerSer: 4.748 ± 0.087
3.466SerThr: 3.466 ± 0.071
4.328SerVal: 4.328 ± 0.072
0.853SerTrp: 0.853 ± 0.036
2.077SerTyr: 2.077 ± 0.055
0.001SerXaa: 0.001 ± 0.001
Thr
4.62ThrAla: 4.62 ± 0.079
0.516ThrCys: 0.516 ± 0.023
2.869ThrAsp: 2.869 ± 0.059
3.197ThrGlu: 3.197 ± 0.063
1.974ThrPhe: 1.974 ± 0.051
4.201ThrGly: 4.201 ± 0.085
1.24ThrHis: 1.24 ± 0.043
3.473ThrIle: 3.473 ± 0.071
2.505ThrLys: 2.505 ± 0.058
6.011ThrLeu: 6.011 ± 0.098
1.248ThrMet: 1.248 ± 0.048
1.948ThrAsn: 1.948 ± 0.052
2.852ThrPro: 2.852 ± 0.059
2.256ThrGln: 2.256 ± 0.057
2.446ThrArg: 2.446 ± 0.051
3.122ThrSer: 3.122 ± 0.055
2.878ThrThr: 2.878 ± 0.063
3.634ThrVal: 3.634 ± 0.074
0.607ThrTrp: 0.607 ± 0.029
1.417ThrTyr: 1.417 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
5.437ValAla: 5.437 ± 0.109
0.855ValCys: 0.855 ± 0.035
3.703ValAsp: 3.703 ± 0.069
3.706ValGlu: 3.706 ± 0.073
3.179ValPhe: 3.179 ± 0.082
4.048ValGly: 4.048 ± 0.067
1.325ValHis: 1.325 ± 0.047
4.784ValIle: 4.784 ± 0.074
3.468ValLys: 3.468 ± 0.068
6.589ValLeu: 6.589 ± 0.093
1.774ValMet: 1.774 ± 0.047
2.723ValAsn: 2.723 ± 0.064
2.472ValPro: 2.472 ± 0.055
2.403ValGln: 2.403 ± 0.052
2.748ValArg: 2.748 ± 0.058
4.539ValSer: 4.539 ± 0.084
3.638ValThr: 3.638 ± 0.069
4.448ValVal: 4.448 ± 0.093
0.768ValTrp: 0.768 ± 0.03
1.825ValTyr: 1.825 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
0.916TrpAla: 0.916 ± 0.038
0.152TrpCys: 0.152 ± 0.013
0.568TrpAsp: 0.568 ± 0.027
0.632TrpGlu: 0.632 ± 0.025
0.604TrpPhe: 0.604 ± 0.029
0.812TrpGly: 0.812 ± 0.035
0.364TrpHis: 0.364 ± 0.022
0.747TrpIle: 0.747 ± 0.028
0.603TrpLys: 0.603 ± 0.029
1.667TrpLeu: 1.667 ± 0.046
0.362TrpMet: 0.362 ± 0.022
0.485TrpAsn: 0.485 ± 0.027
0.437TrpPro: 0.437 ± 0.025
0.853TrpGln: 0.853 ± 0.033
0.687TrpArg: 0.687 ± 0.03
0.668TrpSer: 0.668 ± 0.033
0.545TrpThr: 0.545 ± 0.029
0.874TrpVal: 0.874 ± 0.032
0.176TrpTrp: 0.176 ± 0.015
0.324TrpTyr: 0.324 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.497TyrAla: 2.497 ± 0.055
0.448TyrCys: 0.448 ± 0.023
1.581TyrAsp: 1.581 ± 0.046
1.442TyrGlu: 1.442 ± 0.044
1.52TyrPhe: 1.52 ± 0.045
1.919TyrGly: 1.919 ± 0.047
0.82TyrHis: 0.82 ± 0.031
1.911TyrIle: 1.911 ± 0.048
1.68TyrLys: 1.68 ± 0.049
3.492TyrLeu: 3.492 ± 0.072
0.704TyrMet: 0.704 ± 0.028
1.141TyrAsn: 1.141 ± 0.037
1.44TyrPro: 1.44 ± 0.04
1.841TyrGln: 1.841 ± 0.049
1.725TyrArg: 1.725 ± 0.042
2.154TyrSer: 2.154 ± 0.057
1.612TyrThr: 1.612 ± 0.045
1.704TyrVal: 1.704 ± 0.048
0.464TyrTrp: 0.464 ± 0.023
1.184TyrTyr: 1.184 ± 0.047
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.001XaaPhe: 0.001 ± 0.001
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.001XaaLeu: 0.001 ± 0.001
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5290 proteins (782836 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski