Amino acid dipepetide frequency for Enterobacteria phage mEp021

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.774AlaAla: 9.774 ± 1.422
1.128AlaCys: 1.128 ± 0.292
5.513AlaAsp: 5.513 ± 0.675
6.077AlaGlu: 6.077 ± 0.744
3.258AlaPhe: 3.258 ± 0.371
5.513AlaGly: 5.513 ± 0.42
1.253AlaHis: 1.253 ± 0.313
7.456AlaIle: 7.456 ± 0.874
4.887AlaLys: 4.887 ± 0.561
6.203AlaLeu: 6.203 ± 0.508
2.193AlaMet: 2.193 ± 0.411
4.323AlaAsn: 4.323 ± 0.793
1.88AlaPro: 1.88 ± 0.365
4.01AlaGln: 4.01 ± 0.754
5.889AlaArg: 5.889 ± 0.731
3.947AlaSer: 3.947 ± 1.042
4.762AlaThr: 4.762 ± 0.616
5.701AlaVal: 5.701 ± 0.678
0.627AlaTrp: 0.627 ± 0.203
2.757AlaTyr: 2.757 ± 0.39
0.0AlaXaa: 0.0 ± 0.0
Cys
1.19CysAla: 1.19 ± 0.422
0.376CysCys: 0.376 ± 0.186
1.441CysAsp: 1.441 ± 0.362
0.752CysGlu: 0.752 ± 0.229
0.439CysPhe: 0.439 ± 0.159
1.754CysGly: 1.754 ± 0.397
0.251CysHis: 0.251 ± 0.129
0.877CysIle: 0.877 ± 0.207
1.629CysLys: 1.629 ± 0.626
1.441CysLeu: 1.441 ± 0.345
0.313CysMet: 0.313 ± 0.161
0.689CysAsn: 0.689 ± 0.259
0.689CysPro: 0.689 ± 0.321
0.376CysGln: 0.376 ± 0.183
1.692CysArg: 1.692 ± 0.431
0.814CysSer: 0.814 ± 0.254
0.627CysThr: 0.627 ± 0.192
1.002CysVal: 1.002 ± 0.299
0.313CysTrp: 0.313 ± 0.138
0.501CysTyr: 0.501 ± 0.16
0.0CysXaa: 0.0 ± 0.0
Asp
5.952AspAla: 5.952 ± 0.635
0.752AspCys: 0.752 ± 0.245
4.135AspAsp: 4.135 ± 0.6
5.388AspGlu: 5.388 ± 0.775
2.381AspPhe: 2.381 ± 0.391
5.639AspGly: 5.639 ± 0.488
1.253AspHis: 1.253 ± 0.336
2.569AspIle: 2.569 ± 0.398
4.323AspLys: 4.323 ± 0.525
5.701AspLeu: 5.701 ± 0.593
1.566AspMet: 1.566 ± 0.39
3.321AspAsn: 3.321 ± 0.595
3.007AspPro: 3.007 ± 0.405
1.942AspGln: 1.942 ± 0.531
2.569AspArg: 2.569 ± 0.29
3.133AspSer: 3.133 ± 0.544
2.569AspThr: 2.569 ± 0.565
4.323AspVal: 4.323 ± 0.631
0.877AspTrp: 0.877 ± 0.247
2.757AspTyr: 2.757 ± 0.498
0.0AspXaa: 0.0 ± 0.0
Glu
4.95GluAla: 4.95 ± 0.643
1.316GluCys: 1.316 ± 0.328
3.133GluAsp: 3.133 ± 0.551
5.263GluGlu: 5.263 ± 0.756
2.193GluPhe: 2.193 ± 0.385
3.697GluGly: 3.697 ± 0.536
1.128GluHis: 1.128 ± 0.302
6.203GluIle: 6.203 ± 0.696
3.759GluLys: 3.759 ± 0.512
5.263GluLeu: 5.263 ± 0.598
2.068GluMet: 2.068 ± 0.411
3.133GluAsn: 3.133 ± 0.384
1.19GluPro: 1.19 ± 0.205
3.007GluGln: 3.007 ± 0.466
4.198GluArg: 4.198 ± 0.671
3.383GluSer: 3.383 ± 0.68
4.198GluThr: 4.198 ± 0.956
4.01GluVal: 4.01 ± 0.508
1.253GluTrp: 1.253 ± 0.323
2.882GluTyr: 2.882 ± 0.407
0.0GluXaa: 0.0 ± 0.0
Phe
2.255PheAla: 2.255 ± 0.468
0.814PheCys: 0.814 ± 0.224
3.07PheAsp: 3.07 ± 0.38
2.381PheGlu: 2.381 ± 0.346
1.316PhePhe: 1.316 ± 0.254
2.068PheGly: 2.068 ± 0.376
0.752PheHis: 0.752 ± 0.182
1.629PheIle: 1.629 ± 0.338
2.318PheLys: 2.318 ± 0.349
1.88PheLeu: 1.88 ± 0.348
1.065PheMet: 1.065 ± 0.332
2.945PheAsn: 2.945 ± 0.513
1.128PhePro: 1.128 ± 0.327
0.627PheGln: 0.627 ± 0.148
1.754PheArg: 1.754 ± 0.272
2.068PheSer: 2.068 ± 0.484
2.318PheThr: 2.318 ± 0.362
2.005PheVal: 2.005 ± 0.397
0.501PheTrp: 0.501 ± 0.175
1.817PheTyr: 1.817 ± 0.348
0.0PheXaa: 0.0 ± 0.0
Gly
4.762GlyAla: 4.762 ± 0.492
1.88GlyCys: 1.88 ± 0.489
3.884GlyAsp: 3.884 ± 0.588
4.887GlyGlu: 4.887 ± 0.678
2.757GlyPhe: 2.757 ± 0.506
5.325GlyGly: 5.325 ± 0.604
1.378GlyHis: 1.378 ± 0.425
3.822GlyIle: 3.822 ± 0.475
6.14GlyLys: 6.14 ± 0.733
5.388GlyLeu: 5.388 ± 0.568
2.694GlyMet: 2.694 ± 0.462
4.323GlyAsn: 4.323 ± 0.573
1.002GlyPro: 1.002 ± 0.231
1.942GlyGln: 1.942 ± 0.332
4.01GlyArg: 4.01 ± 0.447
3.446GlySer: 3.446 ± 0.497
3.446GlyThr: 3.446 ± 0.481
5.764GlyVal: 5.764 ± 0.55
0.94GlyTrp: 0.94 ± 0.228
3.133GlyTyr: 3.133 ± 0.514
0.0GlyXaa: 0.0 ± 0.0
His
1.19HisAla: 1.19 ± 0.259
0.501HisCys: 0.501 ± 0.207
1.692HisAsp: 1.692 ± 0.345
2.13HisGlu: 2.13 ± 0.613
0.627HisPhe: 0.627 ± 0.157
1.378HisGly: 1.378 ± 0.307
0.564HisHis: 0.564 ± 0.27
1.128HisIle: 1.128 ± 0.272
1.754HisLys: 1.754 ± 0.436
1.253HisLeu: 1.253 ± 0.279
0.313HisMet: 0.313 ± 0.151
0.564HisAsn: 0.564 ± 0.195
0.689HisPro: 0.689 ± 0.209
0.376HisGln: 0.376 ± 0.138
0.564HisArg: 0.564 ± 0.181
0.877HisSer: 0.877 ± 0.265
0.94HisThr: 0.94 ± 0.216
1.128HisVal: 1.128 ± 0.247
0.376HisTrp: 0.376 ± 0.173
0.501HisTyr: 0.501 ± 0.178
0.0HisXaa: 0.0 ± 0.0
Ile
6.829IleAla: 6.829 ± 0.783
0.752IleCys: 0.752 ± 0.23
5.138IleAsp: 5.138 ± 0.68
3.697IleGlu: 3.697 ± 0.392
1.754IlePhe: 1.754 ± 0.366
3.884IleGly: 3.884 ± 0.514
1.002IleHis: 1.002 ± 0.275
3.822IleIle: 3.822 ± 0.423
4.135IleLys: 4.135 ± 0.49
2.882IleLeu: 2.882 ± 0.4
1.566IleMet: 1.566 ± 0.323
4.135IleAsn: 4.135 ± 0.444
2.193IlePro: 2.193 ± 0.393
1.128IleGln: 1.128 ± 0.228
3.383IleArg: 3.383 ± 0.374
5.012IleSer: 5.012 ± 0.994
5.388IleThr: 5.388 ± 0.611
3.509IleVal: 3.509 ± 0.411
0.627IleTrp: 0.627 ± 0.195
1.378IleTyr: 1.378 ± 0.345
0.0IleXaa: 0.0 ± 0.0
Lys
6.892LysAla: 6.892 ± 0.694
1.504LysCys: 1.504 ± 0.477
3.258LysAsp: 3.258 ± 0.415
4.699LysGlu: 4.699 ± 0.633
2.193LysPhe: 2.193 ± 0.304
4.762LysGly: 4.762 ± 0.476
1.065LysHis: 1.065 ± 0.27
3.759LysIle: 3.759 ± 0.644
4.323LysLys: 4.323 ± 0.536
4.323LysLeu: 4.323 ± 0.618
2.005LysMet: 2.005 ± 0.343
2.13LysAsn: 2.13 ± 0.385
2.882LysPro: 2.882 ± 0.467
2.443LysGln: 2.443 ± 0.357
4.636LysArg: 4.636 ± 0.565
2.569LysSer: 2.569 ± 0.539
4.386LysThr: 4.386 ± 0.522
4.699LysVal: 4.699 ± 0.996
1.566LysTrp: 1.566 ± 0.356
2.13LysTyr: 2.13 ± 0.422
0.0LysXaa: 0.0 ± 0.0
Leu
6.015LeuAla: 6.015 ± 0.738
1.253LeuCys: 1.253 ± 0.365
4.448LeuAsp: 4.448 ± 0.827
3.947LeuGlu: 3.947 ± 0.548
2.757LeuPhe: 2.757 ± 0.404
4.386LeuGly: 4.386 ± 0.503
1.441LeuHis: 1.441 ± 0.306
4.95LeuIle: 4.95 ± 0.585
5.889LeuLys: 5.889 ± 0.738
5.764LeuLeu: 5.764 ± 0.861
2.068LeuMet: 2.068 ± 0.291
4.323LeuAsn: 4.323 ± 0.454
2.882LeuPro: 2.882 ± 0.508
1.629LeuGln: 1.629 ± 0.27
4.762LeuArg: 4.762 ± 0.589
5.388LeuSer: 5.388 ± 0.55
3.634LeuThr: 3.634 ± 0.505
4.323LeuVal: 4.323 ± 0.49
1.002LeuTrp: 1.002 ± 0.224
2.255LeuTyr: 2.255 ± 0.383
0.0LeuXaa: 0.0 ± 0.0
Met
2.631MetAla: 2.631 ± 0.511
0.188MetCys: 0.188 ± 0.101
1.128MetAsp: 1.128 ± 0.238
1.441MetGlu: 1.441 ± 0.346
1.128MetPhe: 1.128 ± 0.292
1.19MetGly: 1.19 ± 0.36
0.501MetHis: 0.501 ± 0.163
1.88MetIle: 1.88 ± 0.342
2.506MetLys: 2.506 ± 0.456
2.068MetLeu: 2.068 ± 0.345
0.877MetMet: 0.877 ± 0.237
1.692MetAsn: 1.692 ± 0.358
1.316MetPro: 1.316 ± 0.348
1.002MetGln: 1.002 ± 0.311
1.754MetArg: 1.754 ± 0.382
2.005MetSer: 2.005 ± 0.344
1.441MetThr: 1.441 ± 0.313
2.13MetVal: 2.13 ± 0.349
0.501MetTrp: 0.501 ± 0.223
1.002MetTyr: 1.002 ± 0.271
0.0MetXaa: 0.0 ± 0.0
Asn
4.887AsnAla: 4.887 ± 1.0
0.188AsnCys: 0.188 ± 0.099
3.258AsnAsp: 3.258 ± 0.478
3.258AsnGlu: 3.258 ± 0.516
1.316AsnPhe: 1.316 ± 0.246
5.012AsnGly: 5.012 ± 0.482
1.504AsnHis: 1.504 ± 0.345
2.694AsnIle: 2.694 ± 0.373
3.822AsnLys: 3.822 ± 0.634
3.697AsnLeu: 3.697 ± 0.559
1.378AsnMet: 1.378 ± 0.431
3.321AsnAsn: 3.321 ± 0.505
2.569AsnPro: 2.569 ± 0.564
2.193AsnGln: 2.193 ± 0.333
2.506AsnArg: 2.506 ± 0.371
3.571AsnSer: 3.571 ± 0.564
3.133AsnThr: 3.133 ± 0.465
2.506AsnVal: 2.506 ± 0.524
0.627AsnTrp: 0.627 ± 0.206
1.692AsnTyr: 1.692 ± 0.3
0.0AsnXaa: 0.0 ± 0.0
Pro
2.569ProAla: 2.569 ± 0.411
0.501ProCys: 0.501 ± 0.177
3.571ProAsp: 3.571 ± 0.651
2.694ProGlu: 2.694 ± 0.363
1.065ProPhe: 1.065 ± 0.238
2.506ProGly: 2.506 ± 0.357
0.689ProHis: 0.689 ± 0.245
1.817ProIle: 1.817 ± 0.407
2.13ProLys: 2.13 ± 0.506
2.694ProLeu: 2.694 ± 0.431
1.002ProMet: 1.002 ± 0.289
1.942ProAsn: 1.942 ± 0.334
1.441ProPro: 1.441 ± 0.412
0.752ProGln: 0.752 ± 0.204
1.629ProArg: 1.629 ± 0.329
1.692ProSer: 1.692 ± 0.28
1.754ProThr: 1.754 ± 0.339
3.759ProVal: 3.759 ± 0.502
0.501ProTrp: 0.501 ± 0.213
0.877ProTyr: 0.877 ± 0.248
0.0ProXaa: 0.0 ± 0.0
Gln
3.634GlnAla: 3.634 ± 0.754
0.564GlnCys: 0.564 ± 0.216
1.316GlnAsp: 1.316 ± 0.363
1.504GlnGlu: 1.504 ± 0.395
1.253GlnPhe: 1.253 ± 0.268
1.566GlnGly: 1.566 ± 0.333
0.376GlnHis: 0.376 ± 0.161
2.005GlnIle: 2.005 ± 0.298
1.629GlnLys: 1.629 ± 0.397
2.506GlnLeu: 2.506 ± 0.49
0.94GlnMet: 0.94 ± 0.211
1.065GlnAsn: 1.065 ± 0.256
1.504GlnPro: 1.504 ± 0.354
1.441GlnGln: 1.441 ± 0.597
2.255GlnArg: 2.255 ± 0.46
1.942GlnSer: 1.942 ± 0.421
2.13GlnThr: 2.13 ± 0.396
2.005GlnVal: 2.005 ± 0.411
0.689GlnTrp: 0.689 ± 0.184
1.19GlnTyr: 1.19 ± 0.285
0.0GlnXaa: 0.0 ± 0.0
Arg
4.198ArgAla: 4.198 ± 0.539
1.253ArgCys: 1.253 ± 0.395
3.697ArgAsp: 3.697 ± 0.557
3.509ArgGlu: 3.509 ± 0.609
2.318ArgPhe: 2.318 ± 0.351
4.198ArgGly: 4.198 ± 0.533
1.19ArgHis: 1.19 ± 0.282
3.759ArgIle: 3.759 ± 0.508
3.509ArgLys: 3.509 ± 0.515
4.448ArgLeu: 4.448 ± 0.492
1.942ArgMet: 1.942 ± 0.391
2.569ArgAsn: 2.569 ± 0.474
2.068ArgPro: 2.068 ± 0.389
1.942ArgGln: 1.942 ± 0.455
3.571ArgArg: 3.571 ± 0.628
2.694ArgSer: 2.694 ± 0.41
3.007ArgThr: 3.007 ± 0.507
4.386ArgVal: 4.386 ± 0.512
0.94ArgTrp: 0.94 ± 0.262
2.569ArgTyr: 2.569 ± 0.359
0.0ArgXaa: 0.0 ± 0.0
Ser
5.2SerAla: 5.2 ± 0.615
1.128SerCys: 1.128 ± 0.301
4.323SerAsp: 4.323 ± 0.503
3.195SerGlu: 3.195 ± 0.567
1.88SerPhe: 1.88 ± 0.362
5.325SerGly: 5.325 ± 0.722
1.002SerHis: 1.002 ± 0.276
3.446SerIle: 3.446 ± 0.737
2.945SerLys: 2.945 ± 0.454
4.887SerLeu: 4.887 ± 0.689
1.566SerMet: 1.566 ± 0.332
3.195SerAsn: 3.195 ± 0.565
1.754SerPro: 1.754 ± 0.307
2.005SerGln: 2.005 ± 0.33
3.007SerArg: 3.007 ± 0.436
2.381SerSer: 2.381 ± 0.744
3.007SerThr: 3.007 ± 0.618
4.636SerVal: 4.636 ± 0.552
0.689SerTrp: 0.689 ± 0.198
1.817SerTyr: 1.817 ± 0.319
0.0SerXaa: 0.0 ± 0.0
Thr
5.513ThrAla: 5.513 ± 0.639
1.19ThrCys: 1.19 ± 0.29
4.135ThrAsp: 4.135 ± 1.082
3.884ThrGlu: 3.884 ± 0.554
1.378ThrPhe: 1.378 ± 0.269
4.95ThrGly: 4.95 ± 0.544
1.002ThrHis: 1.002 ± 0.259
3.571ThrIle: 3.571 ± 0.368
2.945ThrLys: 2.945 ± 0.432
4.699ThrLeu: 4.699 ± 0.792
1.378ThrMet: 1.378 ± 0.319
2.757ThrAsn: 2.757 ± 0.72
3.007ThrPro: 3.007 ± 0.409
1.441ThrGln: 1.441 ± 0.283
2.945ThrArg: 2.945 ± 0.416
3.383ThrSer: 3.383 ± 0.571
2.443ThrThr: 2.443 ± 0.513
3.571ThrVal: 3.571 ± 0.506
0.814ThrTrp: 0.814 ± 0.218
2.13ThrTyr: 2.13 ± 0.424
0.0ThrXaa: 0.0 ± 0.0
Val
5.513ValAla: 5.513 ± 0.701
1.19ValCys: 1.19 ± 0.419
4.135ValAsp: 4.135 ± 0.527
4.574ValGlu: 4.574 ± 0.671
2.945ValPhe: 2.945 ± 0.403
4.386ValGly: 4.386 ± 0.642
0.94ValHis: 0.94 ± 0.298
4.511ValIle: 4.511 ± 0.686
4.135ValLys: 4.135 ± 0.444
4.448ValLeu: 4.448 ± 0.737
2.318ValMet: 2.318 ± 0.359
4.574ValAsn: 4.574 ± 0.639
2.381ValPro: 2.381 ± 0.314
1.441ValGln: 1.441 ± 0.257
2.631ValArg: 2.631 ± 0.434
4.574ValSer: 4.574 ± 0.54
4.574ValThr: 4.574 ± 0.597
4.95ValVal: 4.95 ± 0.853
1.316ValTrp: 1.316 ± 0.272
2.443ValTyr: 2.443 ± 0.428
0.0ValXaa: 0.0 ± 0.0
Trp
0.877TrpAla: 0.877 ± 0.235
0.188TrpCys: 0.188 ± 0.115
0.877TrpAsp: 0.877 ± 0.236
0.94TrpGlu: 0.94 ± 0.241
0.689TrpPhe: 0.689 ± 0.214
0.564TrpGly: 0.564 ± 0.214
0.313TrpHis: 0.313 ± 0.147
0.627TrpIle: 0.627 ± 0.188
1.002TrpLys: 1.002 ± 0.313
1.441TrpLeu: 1.441 ± 0.3
0.439TrpMet: 0.439 ± 0.159
0.313TrpAsn: 0.313 ± 0.124
0.313TrpPro: 0.313 ± 0.18
0.752TrpGln: 0.752 ± 0.25
1.378TrpArg: 1.378 ± 0.327
1.817TrpSer: 1.817 ± 0.452
0.94TrpThr: 0.94 ± 0.262
0.814TrpVal: 0.814 ± 0.23
0.313TrpTrp: 0.313 ± 0.132
0.439TrpTyr: 0.439 ± 0.178
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.318TyrAla: 2.318 ± 0.453
0.501TyrCys: 0.501 ± 0.15
2.068TyrAsp: 2.068 ± 0.431
2.005TyrGlu: 2.005 ± 0.295
1.065TyrPhe: 1.065 ± 0.292
2.819TyrGly: 2.819 ± 0.501
0.94TyrHis: 0.94 ± 0.222
1.692TyrIle: 1.692 ± 0.278
2.443TyrLys: 2.443 ± 0.403
2.13TyrLeu: 2.13 ± 0.44
0.627TyrMet: 0.627 ± 0.223
1.817TyrAsn: 1.817 ± 0.246
1.692TyrPro: 1.692 ± 0.347
1.065TyrGln: 1.065 ± 0.206
2.631TyrArg: 2.631 ± 0.385
2.694TyrSer: 2.694 ± 0.404
2.443TyrThr: 2.443 ± 0.395
2.757TyrVal: 2.757 ± 0.356
0.564TyrTrp: 0.564 ± 0.231
1.378TyrTyr: 1.378 ± 0.282
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (15962 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski