Shigella phage Sf11 SMD-2017

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291AXD9|A0A291AXD9_9CAUD Uncharacterized protein OS=Shigella phage Sf11 SMD-2017 OX=2282196 GN=Sf11_gp27 PE=4 SV=1
MM1 pKa = 7.84IPLLWILSAYY11 pKa = 10.12AFARR15 pKa = 11.84VFEE18 pKa = 4.94ADD20 pKa = 3.03TLYY23 pKa = 11.42QMICYY28 pKa = 10.01GAMFCLSGAALAFMDD43 pKa = 5.54DD44 pKa = 4.37VISDD48 pKa = 3.56

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291AXJ6|A0A291AXJ6_9CAUD DNA-binding domain-containing protein OS=Shigella phage Sf11 SMD-2017 OX=2282196 GN=Sf11_gp71 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 9.86TIYY6 pKa = 9.96RR7 pKa = 11.84RR8 pKa = 11.84EE9 pKa = 4.01KK10 pKa = 10.21LEE12 pKa = 3.76QEE14 pKa = 4.24LGHH17 pKa = 7.05VGAQNFMSKK26 pKa = 7.49QARR29 pKa = 11.84NAMEE33 pKa = 4.66SIRR36 pKa = 11.84VNRR39 pKa = 11.84VVRR42 pKa = 11.84VFNGEE47 pKa = 3.69GKK49 pKa = 10.08RR50 pKa = 11.84RR51 pKa = 11.84VMDD54 pKa = 3.54EE55 pKa = 4.36LIIVFF60 pKa = 4.5

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

14002

31

828

175.0

19.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.549 ± 0.603

1.343 ± 0.164

6.042 ± 0.218

6.635 ± 0.344

3.485 ± 0.196

7.149 ± 0.273

1.6 ± 0.169

6.835 ± 0.213

6.271 ± 0.334

6.999 ± 0.184

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.207 ± 0.205

4.949 ± 0.27

3.442 ± 0.207

3.649 ± 0.294

5.114 ± 0.258

6.585 ± 0.273

5.621 ± 0.393

7.092 ± 0.298

1.621 ± 0.114

3.814 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski