Saprochaete ingens

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Dipodascaceae; Saprochaete

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6465 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5E8BHE6|A0A5E8BHE6_9ASCO Uncharacterized protein OS=Saprochaete ingens OX=2606893 GN=SAPINGB_P002975 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.38FSDD5 pKa = 3.56ILFSTSAVMMVNALPSNILFPKK27 pKa = 9.97RR28 pKa = 11.84QVLEE32 pKa = 4.42DD33 pKa = 3.6YY34 pKa = 11.04NIISVQGSSSTSTLSAVPTSSLNSTSQYY62 pKa = 11.21NLNNSTATLNSTLLQNSTTTTLAPYY87 pKa = 9.37TNATVVVTEE96 pKa = 4.84FGTPVTKK103 pKa = 9.92TVLSIVTQVVIVDD116 pKa = 3.43SDD118 pKa = 4.16GNVITGEE125 pKa = 4.1YY126 pKa = 9.82NDD128 pKa = 4.65AGANEE133 pKa = 4.11NSVAASVSSGAGSQVVTVSVDD154 pKa = 3.22AASAAATSSSDD165 pKa = 3.35SSSSSSTSVSAGSDD179 pKa = 3.17TSSSNGSSDD188 pKa = 3.23SSGSNSSSGSSSTSNSSGSSDD209 pKa = 3.23SSSNSLGSSDD219 pKa = 4.23SLSSSSSSSSDD230 pKa = 3.14SSSSSSNTSSGTSSSSSGSGSGSSEE255 pKa = 3.69TSANTSSSSSSASSDD270 pKa = 3.18SSSTNNEE277 pKa = 3.78SSGSSGSSSNTGSSSSPLATLSANLQLVPVSSSTSTSSSDD317 pKa = 3.15EE318 pKa = 4.12STKK321 pKa = 10.93SSVIEE326 pKa = 3.99STISSSSSSSSGSNAIEE343 pKa = 4.31SFSSSGSNTILSSSGSNTIEE363 pKa = 4.13SSSSSSTSTSVSSFSSDD380 pKa = 2.95SPFFVAVTTILPGSATIPTSSPASPSSPSSSSSTSPSLASNADD423 pKa = 3.18SAAEE427 pKa = 3.95ITSKK431 pKa = 10.64ISGLVSSSSSSIITHH446 pKa = 5.48SAYY449 pKa = 11.06LNATVTTAASTASTISSNSTVLAHH473 pKa = 6.85SISSSTATALSTSTFSTLNATMEE496 pKa = 4.16FALKK500 pKa = 8.71HH501 pKa = 4.64THH503 pKa = 5.87SASATSATSAATTTAPASSTAAPSLSKK530 pKa = 10.7LVEE533 pKa = 4.0TSKK536 pKa = 10.13TNVSTVSSSATDD548 pKa = 3.44EE549 pKa = 4.29SSSFSAVTLSLGSIVAANFSTEE571 pKa = 3.66ISGSADD577 pKa = 2.85KK578 pKa = 10.23STASISSVEE587 pKa = 4.03YY588 pKa = 9.59STSLSDD594 pKa = 4.13ASVTEE599 pKa = 4.19SAVASAIEE607 pKa = 4.14SSVEE611 pKa = 3.79STIASSVSSSAEE623 pKa = 3.85VPTEE627 pKa = 3.57SSYY630 pKa = 11.21ISSSEE635 pKa = 4.07SSSAAPIVSSVEE647 pKa = 4.03SSVDD651 pKa = 3.22PTTEE655 pKa = 3.58SSVISEE661 pKa = 4.19STAISSSSTDD671 pKa = 3.12LSSFFSVEE679 pKa = 4.01SSVEE683 pKa = 3.83SDD685 pKa = 4.38PISSTSVSSSSSSSSFSATTIDD707 pKa = 3.58SLEE710 pKa = 4.27SFTDD714 pKa = 3.5AASVNVANKK723 pKa = 9.6QNEE726 pKa = 4.08ITIVATDD733 pKa = 3.57SSSASSSSSSSSSSSEE749 pKa = 3.99WIVSAATLSVNAVEE763 pKa = 4.92PSAAQPSSSTADD775 pKa = 3.6PQSSTGAASTAADD788 pKa = 3.43SAADD792 pKa = 3.54VVVALGAGATDD803 pKa = 3.77AAGQVINTVQQQTSVSVQTVNFDD826 pKa = 3.47PTTTSSSSSSAVVVVVEE843 pKa = 4.62TPVQEE848 pKa = 4.51AQTSTSSTWVPEE860 pKa = 3.99AVPTTSSTTYY870 pKa = 9.66TPEE873 pKa = 4.01AVPTTSSTSTPEE885 pKa = 3.88AVPTTSSTSTPDD897 pKa = 3.3AVPTTSTTSTPDD909 pKa = 3.21AVPTTSSQVIATTSSTAAAASTTTSTSSDD938 pKa = 2.35GWTDD942 pKa = 3.14YY943 pKa = 11.08DD944 pKa = 4.16YY945 pKa = 11.39PGDD948 pKa = 4.56DD949 pKa = 4.16GLFKK953 pKa = 9.87FTPRR957 pKa = 11.84SLSYY961 pKa = 10.91TPFNNDD967 pKa = 2.67TSCRR971 pKa = 11.84STSEE975 pKa = 3.7IAQDD979 pKa = 5.36LADD982 pKa = 5.33LYY984 pKa = 11.49NKK986 pKa = 10.1GIRR989 pKa = 11.84EE990 pKa = 4.02LRR992 pKa = 11.84LYY994 pKa = 10.81GADD997 pKa = 3.39CDD999 pKa = 4.77AINTILSPAKK1009 pKa = 10.27AQGLTVIMGFYY1020 pKa = 8.45VTSAGVDD1027 pKa = 3.62VVDD1030 pKa = 4.83SEE1032 pKa = 4.74VEE1034 pKa = 4.3SFISWAQSSGDD1045 pKa = 3.4IDD1047 pKa = 3.99VVTAVAIGNEE1057 pKa = 3.99AVTNGWVTADD1067 pKa = 3.82DD1068 pKa = 4.55LASKK1072 pKa = 9.85MEE1074 pKa = 4.25SVRR1077 pKa = 11.84SKK1079 pKa = 10.42LQSEE1083 pKa = 4.31IGFTGPVITSEE1094 pKa = 3.86IVGIYY1099 pKa = 9.41QSYY1102 pKa = 8.75PQLCTSSAADD1112 pKa = 3.35YY1113 pKa = 10.8VGVNIHH1119 pKa = 7.1PYY1121 pKa = 9.4FDD1123 pKa = 3.5SGIAADD1129 pKa = 3.71QSGDD1133 pKa = 3.31FMLAQVQLVKK1143 pKa = 10.51DD1144 pKa = 3.28ACGRR1148 pKa = 11.84SDD1150 pKa = 3.73VKK1152 pKa = 11.13VLEE1155 pKa = 4.03TGYY1158 pKa = 9.83PHH1160 pKa = 7.93DD1161 pKa = 4.83GNTNGLQVPSDD1172 pKa = 4.1VNQGIAVTQIYY1183 pKa = 10.01NALQGDD1189 pKa = 4.46VYY1191 pKa = 10.04MFSMWDD1197 pKa = 3.7DD1198 pKa = 3.03HH1199 pKa = 7.02WKK1201 pKa = 10.63DD1202 pKa = 3.1AGNYY1206 pKa = 7.65NVEE1209 pKa = 3.97WYY1211 pKa = 10.38FGLFYY1216 pKa = 10.85RR1217 pKa = 11.84FPP1219 pKa = 5.33

Molecular weight: 121.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5E8BV45|A0A5E8BV45_9ASCO Uncharacterized protein OS=Saprochaete ingens OX=2606893 GN=SAPINGB_P004359 PE=4 SV=1
MM1 pKa = 7.82LSRR4 pKa = 11.84FAAIRR9 pKa = 11.84PAMRR13 pKa = 11.84VVASGVASRR22 pKa = 11.84PLTLLAAQSNPLTALRR38 pKa = 11.84ARR40 pKa = 11.84MTPGSPTAALVSPLAQPNPLLLPALPDD67 pKa = 3.81LLDD70 pKa = 3.32QRR72 pKa = 11.84RR73 pKa = 11.84WKK75 pKa = 10.92ARR77 pKa = 11.84GNTYY81 pKa = 10.27QPSTLKK87 pKa = 10.36RR88 pKa = 11.84KK89 pKa = 10.0RR90 pKa = 11.84INGFLARR97 pKa = 11.84LKK99 pKa = 9.8TRR101 pKa = 11.84GGRR104 pKa = 11.84KK105 pKa = 8.7VLARR109 pKa = 11.84RR110 pKa = 11.84KK111 pKa = 8.08TKK113 pKa = 10.28GRR115 pKa = 11.84WFLSHH120 pKa = 7.05

Molecular weight: 13.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6465

0

6465

3935327

66

7175

608.7

67.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.571 ± 0.062

0.964 ± 0.011

5.465 ± 0.033

6.217 ± 0.042

3.79 ± 0.023

5.688 ± 0.039

2.294 ± 0.016

5.418 ± 0.025

5.533 ± 0.036

8.431 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.752 ± 0.013

4.94 ± 0.028

5.605 ± 0.037

4.549 ± 0.046

4.665 ± 0.03

10.831 ± 0.108

6.505 ± 0.036

5.875 ± 0.028

0.941 ± 0.009

2.966 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski