Pseudoalteromonas sp. P1-26

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Pseudoalteromonadaceae; Pseudoalteromonas; unclassified Pseudoalteromonas

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4183 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q0I066|A0A0Q0I066_9GAMM Catalase OS=Pseudoalteromonas sp. P1-26 OX=1723759 GN=katB PE=3 SV=1
MM1 pKa = 7.63QITRR5 pKa = 11.84WFFTTILVVLFSGLSYY21 pKa = 10.85SAVADD26 pKa = 3.6VVPAGTVIKK35 pKa = 9.87NQASATYY42 pKa = 7.69RR43 pKa = 11.84TCIDD47 pKa = 4.34DD48 pKa = 3.91SCSQATEE55 pKa = 4.06EE56 pKa = 5.23RR57 pKa = 11.84SITSNIVEE65 pKa = 4.28TLIQGVPAFEE75 pKa = 4.5FLTDD79 pKa = 3.33QRR81 pKa = 11.84LPAISDD87 pKa = 3.49RR88 pKa = 11.84PVYY91 pKa = 9.63FAHH94 pKa = 6.93TISNIGNVADD104 pKa = 5.2RR105 pKa = 11.84YY106 pKa = 8.86QICVSAVDD114 pKa = 3.79AQVSEE119 pKa = 3.85WRR121 pKa = 11.84VYY123 pKa = 11.55ADD125 pKa = 3.2TDD127 pKa = 3.93LDD129 pKa = 3.8GRR131 pKa = 11.84PDD133 pKa = 3.76PGQILLSSDD142 pKa = 4.21DD143 pKa = 4.49GSACLPNLTNSIDD156 pKa = 3.78PSEE159 pKa = 4.12TWGLVIEE166 pKa = 4.89VIANGAAGSVLLSAIEE182 pKa = 4.29LEE184 pKa = 4.52ATSSDD189 pKa = 4.67DD190 pKa = 3.29NTLNQTVVDD199 pKa = 3.91SVEE202 pKa = 4.7FIEE205 pKa = 5.4GPLIEE210 pKa = 4.75VLKK213 pKa = 10.83SMPVRR218 pKa = 11.84VGGSPSGPYY227 pKa = 8.31TVRR230 pKa = 11.84LAYY233 pKa = 10.57RR234 pKa = 11.84NVSNFTASSVVLEE247 pKa = 5.54DD248 pKa = 3.6ILPTTFFDD256 pKa = 5.06GQGQPQTGGFEE267 pKa = 4.27YY268 pKa = 11.06VPGSGRR274 pKa = 11.84WSQTGNTALTDD285 pKa = 4.06DD286 pKa = 4.82DD287 pKa = 5.65DD288 pKa = 4.61GVEE291 pKa = 4.35GGGTEE296 pKa = 3.91PASYY300 pKa = 10.14CAYY303 pKa = 10.49DD304 pKa = 3.61GTAANVDD311 pKa = 3.52CQDD314 pKa = 3.02RR315 pKa = 11.84VRR317 pKa = 11.84FEE319 pKa = 4.68LATLPPGGEE328 pKa = 4.4GYY330 pKa = 9.92IEE332 pKa = 4.31FAVQVVGGISTGGRR346 pKa = 11.84ILNIAGYY353 pKa = 10.2YY354 pKa = 8.99YY355 pKa = 10.92NNEE358 pKa = 4.39DD359 pKa = 3.33NTVVYY364 pKa = 9.46AQSGSGPVGGAITPFDD380 pKa = 4.04TNVVPFQIKK389 pKa = 10.16SSATAPSVVVNDD401 pKa = 3.62SSTDD405 pKa = 3.25INIGVDD411 pKa = 3.53DD412 pKa = 4.84SLDD415 pKa = 3.36SGNRR419 pKa = 11.84VEE421 pKa = 5.57IAAAGQGAVIRR432 pKa = 11.84FTNYY436 pKa = 8.14IWNTGDD442 pKa = 3.91GVDD445 pKa = 3.84TFDD448 pKa = 5.95LNVDD452 pKa = 3.59RR453 pKa = 11.84ALNRR457 pKa = 11.84VGASLANPFPDD468 pKa = 3.34NAIVRR473 pKa = 11.84VLQADD478 pKa = 4.28GATPLLDD485 pKa = 3.41TSNSGRR491 pKa = 11.84VDD493 pKa = 3.41TGPIPLPNFDD503 pKa = 4.25TGACSGRR510 pKa = 11.84FVTSADD516 pKa = 3.25NTRR519 pKa = 11.84CGLPVVVEE527 pKa = 4.24VEE529 pKa = 4.33LPADD533 pKa = 3.67AVGGPYY539 pKa = 10.61EE540 pKa = 4.3LTLAATSNVDD550 pKa = 3.41TTVTNAVSNILLSVVPATVDD570 pKa = 2.98ITNNLPLSAGSAPGAGVGPEE590 pKa = 3.98PTAVTTISLMPGNTGTFTLYY610 pKa = 11.06VNNTGDD616 pKa = 4.34RR617 pKa = 11.84QDD619 pKa = 3.55QYY621 pKa = 11.98QLAASTTNFAAGTLYY636 pKa = 10.22PGWLVSFYY644 pKa = 10.65RR645 pKa = 11.84DD646 pKa = 3.55GGAGDD651 pKa = 4.13CTSLGNAIDD660 pKa = 3.72VTDD663 pKa = 5.48IIPAGSAQLVCAHH676 pKa = 5.94VQTAANAEE684 pKa = 3.88VDD686 pKa = 3.95VYY688 pKa = 11.6DD689 pKa = 4.22LYY691 pKa = 11.42FRR693 pKa = 11.84ASSLLSAAQDD703 pKa = 3.18IKK705 pKa = 11.32LDD707 pKa = 3.51AVEE710 pKa = 4.86ILGAPDD716 pKa = 3.46ISFSPDD722 pKa = 2.58QFNQVVPGSSVSYY735 pKa = 8.02THH737 pKa = 6.58QIKK740 pKa = 8.18NTGTVPLSSIQLTATPQAEE759 pKa = 4.83DD760 pKa = 3.71DD761 pKa = 4.2NGWAVLVYY769 pKa = 10.29EE770 pKa = 4.92DD771 pKa = 5.31DD772 pKa = 4.6GNGVWGPEE780 pKa = 4.24DD781 pKa = 3.37EE782 pKa = 4.74TIPLGGVLGTDD793 pKa = 3.23NGDD796 pKa = 3.8EE797 pKa = 3.87ILLPGEE803 pKa = 4.66SITVHH808 pKa = 5.0VRR810 pKa = 11.84VFAPASAVLGDD821 pKa = 4.1INVKK825 pKa = 9.72TITLTTTVGSDD836 pKa = 2.93TLEE839 pKa = 4.25RR840 pKa = 11.84SVTDD844 pKa = 3.26STSTSQGNTLIFKK857 pKa = 9.49EE858 pKa = 3.89QALDD862 pKa = 3.96SNCDD866 pKa = 3.51GVPDD870 pKa = 4.98GPLACTGDD878 pKa = 3.92SCFSTSVFPVHH889 pKa = 7.14PGQCVLYY896 pKa = 10.28RR897 pKa = 11.84LTARR901 pKa = 11.84NQSAEE906 pKa = 3.88PVFNVLINDD915 pKa = 3.76RR916 pKa = 11.84TQAYY920 pKa = 5.48TTYY923 pKa = 10.37FQTALNCSSPSGSCNADD940 pKa = 2.73VTTPNDD946 pKa = 3.59GGVGDD951 pKa = 4.11IQADD955 pKa = 3.43IGVLQAGEE963 pKa = 3.79QAILIFGVRR972 pKa = 11.84VRR974 pKa = 4.25

Molecular weight: 101.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q0J7Z7|A0A0Q0J7Z7_9GAMM Ribose-phosphate pyrophosphokinase OS=Pseudoalteromonas sp. P1-26 OX=1723759 GN=prs_1 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 8.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.67VLSAA44 pKa = 4.05

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4183

0

4183

1392368

29

2234

332.9

37.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.853 ± 0.043

0.989 ± 0.013

5.741 ± 0.04

6.213 ± 0.036

4.383 ± 0.022

6.401 ± 0.039

2.205 ± 0.02

6.296 ± 0.035

5.701 ± 0.035

10.414 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.362 ± 0.018

4.656 ± 0.026

3.638 ± 0.021

4.792 ± 0.035

3.978 ± 0.029

6.785 ± 0.035

5.401 ± 0.029

6.757 ± 0.028

1.153 ± 0.014

3.283 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski