Amino acid dipepetide frequency for Pseudoalteromonas sp. P1-26

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.81AlaAla: 7.81 ± 0.098	0.968AlaCys: 0.968 ± 0.031	5.156AlaAsp: 5.156 ± 0.069	5.626AlaGlu: 5.626 ± 0.07	3.505AlaPhe: 3.505 ± 0.049	5.808AlaGly: 5.808 ± 0.079	1.762AlaHis: 1.762 ± 0.04	6.174AlaIle: 6.174 ± 0.067	5.649AlaLys: 5.649 ± 0.079	9.899AlaLeu: 9.899 ± 0.102	2.54AlaMet: 2.54 ± 0.045	4.06AlaAsn: 4.06 ± 0.062	2.907AlaPro: 2.907 ± 0.057	3.931AlaGln: 3.931 ± 0.065	3.384AlaArg: 3.384 ± 0.051	5.504AlaSer: 5.504 ± 0.073	4.571AlaThr: 4.571 ± 0.055	5.848AlaVal: 5.848 ± 0.074	0.952AlaTrp: 0.952 ± 0.028	2.473AlaTyr: 2.473 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.81CysAla: 0.81 ± 0.025	0.167CysCys: 0.167 ± 0.012	0.611CysAsp: 0.611 ± 0.023	0.643CysGlu: 0.643 ± 0.022	0.467CysPhe: 0.467 ± 0.018	0.71CysGly: 0.71 ± 0.022	0.314CysHis: 0.314 ± 0.016	0.615CysIle: 0.615 ± 0.025	0.48CysLys: 0.48 ± 0.02	1.006CysLeu: 1.006 ± 0.026	0.217CysMet: 0.217 ± 0.014	0.385CysAsn: 0.385 ± 0.018	0.419CysPro: 0.419 ± 0.023	0.483CysGln: 0.483 ± 0.018	0.38CysArg: 0.38 ± 0.014	0.667CysSer: 0.667 ± 0.022	0.437CysThr: 0.437 ± 0.017	0.612CysVal: 0.612 ± 0.022	0.102CysTrp: 0.102 ± 0.009	0.365CysTyr: 0.365 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	4.794AspAla: 4.794 ± 0.074	0.524AspCys: 0.524 ± 0.02	3.596AspAsp: 3.596 ± 0.086	4.375AspGlu: 4.375 ± 0.073	2.726AspPhe: 2.726 ± 0.048	3.784AspGly: 3.784 ± 0.074	1.038AspHis: 1.038 ± 0.027	4.201AspIle: 4.201 ± 0.05	3.72AspLys: 3.72 ± 0.051	5.332AspLeu: 5.332 ± 0.077	1.315AspMet: 1.315 ± 0.03	2.824AspAsn: 2.824 ± 0.058	1.844AspPro: 1.844 ± 0.035	1.849AspGln: 1.849 ± 0.034	1.812AspArg: 1.812 ± 0.04	3.584AspSer: 3.584 ± 0.069	2.893AspThr: 2.893 ± 0.053	4.0AspVal: 4.0 ± 0.064	0.813AspTrp: 0.813 ± 0.023	2.392AspTyr: 2.392 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	4.778GluAla: 4.778 ± 0.073	0.473GluCys: 0.473 ± 0.02	2.804GluAsp: 2.804 ± 0.053	3.259GluGlu: 3.259 ± 0.067	2.853GluPhe: 2.853 ± 0.047	3.346GluGly: 3.346 ± 0.055	1.75GluHis: 1.75 ± 0.038	3.769GluIle: 3.769 ± 0.048	3.953GluLys: 3.953 ± 0.063	7.516GluLeu: 7.516 ± 0.089	1.397GluMet: 1.397 ± 0.037	2.923GluAsn: 2.923 ± 0.046	2.076GluPro: 2.076 ± 0.049	5.188GluGln: 5.188 ± 0.075	2.982GluArg: 2.982 ± 0.048	3.518GluSer: 3.518 ± 0.057	2.886GluThr: 2.886 ± 0.043	4.11GluVal: 4.11 ± 0.061	0.631GluTrp: 0.631 ± 0.021	1.912GluTyr: 1.912 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	4.194PheAla: 4.194 ± 0.062	0.558PheCys: 0.558 ± 0.021	3.077PheAsp: 3.077 ± 0.046	2.654PheGlu: 2.654 ± 0.046	1.765PhePhe: 1.765 ± 0.044	2.959PheGly: 2.959 ± 0.053	0.777PheHis: 0.777 ± 0.024	3.079PheIle: 3.079 ± 0.052	2.619PheLys: 2.619 ± 0.047	3.415PheLeu: 3.415 ± 0.063	1.039PheMet: 1.039 ± 0.026	2.528PheAsn: 2.528 ± 0.043	1.268PhePro: 1.268 ± 0.034	1.125PheGln: 1.125 ± 0.029	1.363PheArg: 1.363 ± 0.033	3.758PheSer: 3.758 ± 0.053	2.68PheThr: 2.68 ± 0.045	2.81PheVal: 2.81 ± 0.046	0.529PheTrp: 0.529 ± 0.022	1.63PheTyr: 1.63 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	4.991GlyAla: 4.991 ± 0.065	0.812GlyCys: 0.812 ± 0.026	3.654GlyAsp: 3.654 ± 0.057	4.128GlyGlu: 4.128 ± 0.059	3.445GlyPhe: 3.445 ± 0.051	4.273GlyGly: 4.273 ± 0.068	1.472GlyHis: 1.472 ± 0.039	4.283GlyIle: 4.283 ± 0.063	3.649GlyLys: 3.649 ± 0.057	6.676GlyLeu: 6.676 ± 0.077	1.712GlyMet: 1.712 ± 0.036	2.558GlyAsn: 2.558 ± 0.05	1.534GlyPro: 1.534 ± 0.037	2.646GlyGln: 2.646 ± 0.041	2.633GlyArg: 2.633 ± 0.046	3.939GlySer: 3.939 ± 0.073	3.313GlyThr: 3.313 ± 0.065	4.896GlyVal: 4.896 ± 0.072	0.811GlyTrp: 0.811 ± 0.024	2.588GlyTyr: 2.588 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	1.629HisAla: 1.629 ± 0.034	0.307HisCys: 0.307 ± 0.015	1.186HisAsp: 1.186 ± 0.033	1.171HisGlu: 1.171 ± 0.03	1.18HisPhe: 1.18 ± 0.033	1.481HisGly: 1.481 ± 0.038	0.656HisHis: 0.656 ± 0.024	1.441HisIle: 1.441 ± 0.034	1.124HisLys: 1.124 ± 0.033	2.213HisLeu: 2.213 ± 0.043	0.457HisMet: 0.457 ± 0.018	0.978HisAsn: 0.978 ± 0.026	1.009HisPro: 1.009 ± 0.028	1.213HisGln: 1.213 ± 0.032	0.846HisArg: 0.846 ± 0.027	1.498HisSer: 1.498 ± 0.034	1.12HisThr: 1.12 ± 0.027	1.279HisVal: 1.279 ± 0.03	0.334HisTrp: 0.334 ± 0.017	0.931HisTyr: 0.931 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.648IleAla: 6.648 ± 0.084	0.647IleCys: 0.647 ± 0.025	4.447IleAsp: 4.447 ± 0.059	4.653IleGlu: 4.653 ± 0.052	2.229IlePhe: 2.229 ± 0.044	4.178IleGly: 4.178 ± 0.069	1.141IleHis: 1.141 ± 0.027	3.593IleIle: 3.593 ± 0.061	3.902IleLys: 3.902 ± 0.053	5.069IleLeu: 5.069 ± 0.075	1.258IleMet: 1.258 ± 0.03	3.623IleAsn: 3.623 ± 0.056	2.286IlePro: 2.286 ± 0.046	1.833IleGln: 1.833 ± 0.038	2.443IleArg: 2.443 ± 0.041	4.893IleSer: 4.893 ± 0.074	3.831IleThr: 3.831 ± 0.062	3.85IleVal: 3.85 ± 0.052	0.619IleTrp: 0.619 ± 0.022	1.814IleTyr: 1.814 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	5.479LysAla: 5.479 ± 0.084	0.33LysCys: 0.33 ± 0.017	2.996LysAsp: 2.996 ± 0.055	3.53LysGlu: 3.53 ± 0.058	1.798LysPhe: 1.798 ± 0.039	3.352LysGly: 3.352 ± 0.049	1.49LysHis: 1.49 ± 0.036	2.945LysIle: 2.945 ± 0.05	3.236LysLys: 3.236 ± 0.06	5.993LysLeu: 5.993 ± 0.072	1.365LysMet: 1.365 ± 0.029	2.463LysAsn: 2.463 ± 0.047	2.508LysPro: 2.508 ± 0.05	3.915LysGln: 3.915 ± 0.062	2.881LysArg: 2.881 ± 0.05	3.496LysSer: 3.496 ± 0.052	3.065LysThr: 3.065 ± 0.052	4.051LysVal: 4.051 ± 0.065	0.577LysTrp: 0.577 ± 0.02	1.539LysTyr: 1.539 ± 0.036	0.0LysXaa: 0.0 ± 0.0
Leu	10.463LeuAla: 10.463 ± 0.099	1.098LeuCys: 1.098 ± 0.033	5.8LeuAsp: 5.8 ± 0.076	6.14LeuGlu: 6.14 ± 0.084	4.539LeuPhe: 4.539 ± 0.073	6.416LeuGly: 6.416 ± 0.076	1.962LeuHis: 1.962 ± 0.041	6.435LeuIle: 6.435 ± 0.079	6.123LeuLys: 6.123 ± 0.079	10.518LeuLeu: 10.518 ± 0.129	2.508LeuMet: 2.508 ± 0.04	5.378LeuAsn: 5.378 ± 0.067	4.322LeuPro: 4.322 ± 0.053	3.721LeuGln: 3.721 ± 0.054	3.779LeuArg: 3.779 ± 0.056	7.916LeuSer: 7.916 ± 0.08	6.242LeuThr: 6.242 ± 0.074	6.969LeuVal: 6.969 ± 0.082	0.929LeuTrp: 0.929 ± 0.026	2.88LeuTyr: 2.88 ± 0.045	0.0LeuXaa: 0.0 ± 0.0
Met	2.331MetAla: 2.331 ± 0.044	0.171MetCys: 0.171 ± 0.013	1.098MetAsp: 1.098 ± 0.026	0.993MetGlu: 0.993 ± 0.027	0.837MetPhe: 0.837 ± 0.027	1.485MetGly: 1.485 ± 0.035	0.521MetHis: 0.521 ± 0.017	1.307MetIle: 1.307 ± 0.035	1.325MetLys: 1.325 ± 0.032	2.662MetLeu: 2.662 ± 0.048	0.651MetMet: 0.651 ± 0.021	1.044MetAsn: 1.044 ± 0.025	1.171MetPro: 1.171 ± 0.03	1.299MetGln: 1.299 ± 0.033	1.079MetArg: 1.079 ± 0.028	1.88MetSer: 1.88 ± 0.037	1.475MetThr: 1.475 ± 0.032	1.523MetVal: 1.523 ± 0.037	0.222MetTrp: 0.222 ± 0.013	0.542MetTyr: 0.542 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.755AsnAla: 3.755 ± 0.055	0.456AsnCys: 0.456 ± 0.018	2.774AsnAsp: 2.774 ± 0.046	2.913AsnGlu: 2.913 ± 0.048	1.778AsnPhe: 1.778 ± 0.04	3.14AsnGly: 3.14 ± 0.059	1.022AsnHis: 1.022 ± 0.028	2.902AsnIle: 2.902 ± 0.048	2.814AsnLys: 2.814 ± 0.051	4.352AsnLeu: 4.352 ± 0.056	1.023AsnMet: 1.023 ± 0.03	2.414AsnAsn: 2.414 ± 0.048	2.086AsnPro: 2.086 ± 0.039	2.362AsnGln: 2.362 ± 0.047	1.924AsnArg: 1.924 ± 0.037	3.032AsnSer: 3.032 ± 0.057	2.679AsnThr: 2.679 ± 0.052	2.782AsnVal: 2.782 ± 0.051	0.635AsnTrp: 0.635 ± 0.022	1.714AsnTyr: 1.714 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	3.034ProAla: 3.034 ± 0.058	0.277ProCys: 0.277 ± 0.015	2.264ProAsp: 2.264 ± 0.043	2.996ProGlu: 2.996 ± 0.051	1.701ProPhe: 1.701 ± 0.039	2.163ProGly: 2.163 ± 0.05	0.844ProHis: 0.844 ± 0.029	2.349ProIle: 2.349 ± 0.043	1.964ProLys: 1.964 ± 0.035	3.893ProLeu: 3.893 ± 0.051	0.855ProMet: 0.855 ± 0.021	1.673ProAsn: 1.673 ± 0.036	0.912ProPro: 0.912 ± 0.027	1.4ProGln: 1.4 ± 0.032	1.207ProArg: 1.207 ± 0.031	2.509ProSer: 2.509 ± 0.045	1.88ProThr: 1.88 ± 0.039	2.747ProVal: 2.747 ± 0.059	0.463ProTrp: 0.463 ± 0.019	1.247ProTyr: 1.247 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	4.733GlnAla: 4.733 ± 0.071	0.403GlnCys: 0.403 ± 0.019	2.081GlnAsp: 2.081 ± 0.044	2.207GlnGlu: 2.207 ± 0.045	2.061GlnPhe: 2.061 ± 0.035	3.237GlnGly: 3.237 ± 0.054	1.27GlnHis: 1.27 ± 0.034	2.598GlnIle: 2.598 ± 0.043	2.17GlnLys: 2.17 ± 0.044	5.636GlnLeu: 5.636 ± 0.089	1.0GlnMet: 1.0 ± 0.027	1.801GlnAsn: 1.801 ± 0.041	1.628GlnPro: 1.628 ± 0.034	3.933GlnGln: 3.933 ± 0.087	1.946GlnArg: 1.946 ± 0.045	3.23GlnSer: 3.23 ± 0.044	2.569GlnThr: 2.569 ± 0.049	3.314GlnVal: 3.314 ± 0.053	0.626GlnTrp: 0.626 ± 0.022	1.479GlnTyr: 1.479 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	3.136ArgAla: 3.136 ± 0.049	0.353ArgCys: 0.353 ± 0.014	2.234ArgAsp: 2.234 ± 0.042	2.577ArgGlu: 2.577 ± 0.053	2.237ArgPhe: 2.237 ± 0.037	2.305ArgGly: 2.305 ± 0.043	0.962ArgHis: 0.962 ± 0.026	2.718ArgIle: 2.718 ± 0.053	2.115ArgLys: 2.115 ± 0.04	4.416ArgLeu: 4.416 ± 0.061	0.924ArgMet: 0.924 ± 0.027	1.647ArgAsn: 1.647 ± 0.032	1.363ArgPro: 1.363 ± 0.032	1.752ArgGln: 1.752 ± 0.037	1.801ArgArg: 1.801 ± 0.043	2.344ArgSer: 2.344 ± 0.039	1.917ArgThr: 1.917 ± 0.036	2.84ArgVal: 2.84 ± 0.044	0.5ArgTrp: 0.5 ± 0.02	1.64ArgTyr: 1.64 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	5.639SerAla: 5.639 ± 0.075	0.677SerCys: 0.677 ± 0.026	3.914SerAsp: 3.914 ± 0.06	4.339SerGlu: 4.339 ± 0.062	3.283SerPhe: 3.283 ± 0.057	4.558SerGly: 4.558 ± 0.077	1.584SerHis: 1.584 ± 0.034	4.154SerIle: 4.154 ± 0.058	3.648SerLys: 3.648 ± 0.06	7.415SerLeu: 7.415 ± 0.087	1.581SerMet: 1.581 ± 0.037	2.97SerAsn: 2.97 ± 0.051	2.26SerPro: 2.26 ± 0.046	3.343SerGln: 3.343 ± 0.054	2.629SerArg: 2.629 ± 0.041	4.635SerSer: 4.635 ± 0.078	3.354SerThr: 3.354 ± 0.056	4.551SerVal: 4.551 ± 0.063	0.855SerTrp: 0.855 ± 0.029	2.458SerTyr: 2.458 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	4.78ThrAla: 4.78 ± 0.063	0.428ThrCys: 0.428 ± 0.016	3.243ThrAsp: 3.243 ± 0.052	3.292ThrGlu: 3.292 ± 0.049	2.163ThrPhe: 2.163 ± 0.044	3.906ThrGly: 3.906 ± 0.064	1.171ThrHis: 1.171 ± 0.031	3.335ThrIle: 3.335 ± 0.051	2.58ThrLys: 2.58 ± 0.048	6.324ThrLeu: 6.324 ± 0.078	1.078ThrMet: 1.078 ± 0.03	2.188ThrAsn: 2.188 ± 0.038	2.646ThrPro: 2.646 ± 0.047	2.427ThrGln: 2.427 ± 0.046	2.066ThrArg: 2.066 ± 0.035	3.478ThrSer: 3.478 ± 0.051	2.977ThrThr: 2.977 ± 0.054	3.776ThrVal: 3.776 ± 0.068	0.593ThrTrp: 0.593 ± 0.02	1.557ThrTyr: 1.557 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	6.289ValAla: 6.289 ± 0.07	0.743ValCys: 0.743 ± 0.028	4.032ValAsp: 4.032 ± 0.069	4.444ValGlu: 4.444 ± 0.075	3.011ValPhe: 3.011 ± 0.044	4.12ValGly: 4.12 ± 0.067	1.176ValHis: 1.176 ± 0.034	4.673ValIle: 4.673 ± 0.057	3.809ValLys: 3.809 ± 0.06	6.582ValLeu: 6.582 ± 0.084	1.793ValMet: 1.793 ± 0.035	3.275ValAsn: 3.275 ± 0.052	2.346ValPro: 2.346 ± 0.047	2.117ValGln: 2.117 ± 0.04	2.491ValArg: 2.491 ± 0.051	4.935ValSer: 4.935 ± 0.065	4.041ValThr: 4.041 ± 0.065	4.902ValVal: 4.902 ± 0.069	0.728ValTrp: 0.728 ± 0.025	2.059ValTyr: 2.059 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	0.72TrpAla: 0.72 ± 0.024	0.128TrpCys: 0.128 ± 0.009	0.551TrpAsp: 0.551 ± 0.019	0.425TrpGlu: 0.425 ± 0.02	0.577TrpPhe: 0.577 ± 0.02	0.677TrpGly: 0.677 ± 0.025	0.399TrpHis: 0.399 ± 0.016	0.503TrpIle: 0.503 ± 0.019	0.335TrpLys: 0.335 ± 0.015	1.719TrpLeu: 1.719 ± 0.039	0.266TrpMet: 0.266 ± 0.015	0.399TrpAsn: 0.399 ± 0.02	0.47TrpPro: 0.47 ± 0.022	1.319TrpGln: 1.319 ± 0.04	0.614TrpArg: 0.614 ± 0.022	0.646TrpSer: 0.646 ± 0.021	0.422TrpThr: 0.422 ± 0.017	0.776TrpVal: 0.776 ± 0.026	0.18TrpTrp: 0.18 ± 0.01	0.407TrpTyr: 0.407 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.51TyrAla: 2.51 ± 0.037	0.372TyrCys: 0.372 ± 0.018	1.892TyrAsp: 1.892 ± 0.043	1.76TyrGlu: 1.76 ± 0.039	1.674TyrPhe: 1.674 ± 0.039	2.113TyrGly: 2.113 ± 0.044	0.743TyrHis: 0.743 ± 0.024	1.882TyrIle: 1.882 ± 0.041	1.795TyrLys: 1.795 ± 0.033	3.503TyrLeu: 3.503 ± 0.056	0.638TyrMet: 0.638 ± 0.019	1.424TyrAsn: 1.424 ± 0.036	1.321TyrPro: 1.321 ± 0.03	2.068TyrGln: 2.068 ± 0.042	1.524TyrArg: 1.524 ± 0.039	2.386TyrSer: 2.386 ± 0.043	1.656TyrThr: 1.656 ± 0.032	1.93TyrVal: 1.93 ± 0.039	0.435TyrTrp: 0.435 ± 0.017	1.204TyrTyr: 1.204 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4183 proteins (1392368 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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