Amino acid dipepetide frequency for Bacillus freudenreichii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.041AlaAla: 7.041 ± 0.103	0.671AlaCys: 0.671 ± 0.025	3.801AlaAsp: 3.801 ± 0.061	5.123AlaGlu: 5.123 ± 0.081	3.45AlaPhe: 3.45 ± 0.059	6.122AlaGly: 6.122 ± 0.089	1.245AlaHis: 1.245 ± 0.035	5.816AlaIle: 5.816 ± 0.085	4.607AlaLys: 4.607 ± 0.074	7.366AlaLeu: 7.366 ± 0.09	2.168AlaMet: 2.168 ± 0.049	2.707AlaAsn: 2.707 ± 0.05	2.197AlaPro: 2.197 ± 0.051	2.179AlaGln: 2.179 ± 0.046	2.839AlaArg: 2.839 ± 0.058	4.142AlaSer: 4.142 ± 0.068	3.354AlaThr: 3.354 ± 0.056	6.035AlaVal: 6.035 ± 0.076	0.641AlaTrp: 0.641 ± 0.03	2.299AlaTyr: 2.299 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.46CysAla: 0.46 ± 0.022	0.08CysCys: 0.08 ± 0.01	0.4CysAsp: 0.4 ± 0.023	0.454CysGlu: 0.454 ± 0.02	0.327CysPhe: 0.327 ± 0.017	0.74CysGly: 0.74 ± 0.028	0.182CysHis: 0.182 ± 0.014	0.507CysIle: 0.507 ± 0.021	0.357CysLys: 0.357 ± 0.017	0.687CysLeu: 0.687 ± 0.024	0.171CysMet: 0.171 ± 0.012	0.281CysAsn: 0.281 ± 0.016	0.363CysPro: 0.363 ± 0.02	0.211CysGln: 0.211 ± 0.016	0.326CysArg: 0.326 ± 0.017	0.507CysSer: 0.507 ± 0.023	0.394CysThr: 0.394 ± 0.021	0.408CysVal: 0.408 ± 0.02	0.069CysTrp: 0.069 ± 0.007	0.238CysTyr: 0.238 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.417AspAla: 3.417 ± 0.064	0.361AspCys: 0.361 ± 0.017	2.556AspAsp: 2.556 ± 0.067	4.583AspGlu: 4.583 ± 0.072	2.435AspPhe: 2.435 ± 0.047	3.453AspGly: 3.453 ± 0.064	1.179AspHis: 1.179 ± 0.031	4.217AspIle: 4.217 ± 0.056	3.166AspLys: 3.166 ± 0.058	5.072AspLeu: 5.072 ± 0.068	1.515AspMet: 1.515 ± 0.037	1.723AspAsn: 1.723 ± 0.039	2.202AspPro: 2.202 ± 0.048	1.945AspGln: 1.945 ± 0.047	2.442AspArg: 2.442 ± 0.045	2.776AspSer: 2.776 ± 0.049	2.384AspThr: 2.384 ± 0.046	3.795AspVal: 3.795 ± 0.061	0.642AspTrp: 0.642 ± 0.024	1.981AspTyr: 1.981 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	5.569GluAla: 5.569 ± 0.074	0.384GluCys: 0.384 ± 0.018	3.875GluAsp: 3.875 ± 0.059	7.122GluGlu: 7.122 ± 0.092	2.599GluPhe: 2.599 ± 0.051	4.526GluGly: 4.526 ± 0.076	1.52GluHis: 1.52 ± 0.038	5.678GluIle: 5.678 ± 0.085	7.134GluLys: 7.134 ± 0.101	7.17GluLeu: 7.17 ± 0.075	2.345GluMet: 2.345 ± 0.039	3.496GluAsn: 3.496 ± 0.053	2.174GluPro: 2.174 ± 0.041	3.069GluGln: 3.069 ± 0.074	3.597GluArg: 3.597 ± 0.062	3.768GluSer: 3.768 ± 0.067	3.858GluThr: 3.858 ± 0.066	4.982GluVal: 4.982 ± 0.078	0.798GluTrp: 0.798 ± 0.023	2.256GluTyr: 2.256 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	3.118PheAla: 3.118 ± 0.052	0.343PheCys: 0.343 ± 0.017	2.333PheAsp: 2.333 ± 0.045	2.844PheGlu: 2.844 ± 0.056	2.437PhePhe: 2.437 ± 0.061	3.503PheGly: 3.503 ± 0.062	0.988PheHis: 0.988 ± 0.031	3.913PheIle: 3.913 ± 0.074	2.394PheLys: 2.394 ± 0.045	4.648PheLeu: 4.648 ± 0.08	1.139PheMet: 1.139 ± 0.03	1.827PheAsn: 1.827 ± 0.042	1.813PhePro: 1.813 ± 0.042	1.52PheGln: 1.52 ± 0.035	1.663PheArg: 1.663 ± 0.033	3.246PheSer: 3.246 ± 0.057	2.586PheThr: 2.586 ± 0.05	3.01PheVal: 3.01 ± 0.057	0.501PheTrp: 0.501 ± 0.023	1.648PheTyr: 1.648 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	5.358GlyAla: 5.358 ± 0.085	0.635GlyCys: 0.635 ± 0.024	3.258GlyAsp: 3.258 ± 0.054	4.667GlyGlu: 4.667 ± 0.063	3.459GlyPhe: 3.459 ± 0.057	5.218GlyGly: 5.218 ± 0.091	1.458GlyHis: 1.458 ± 0.036	6.163GlyIle: 6.163 ± 0.087	5.458GlyLys: 5.458 ± 0.066	6.974GlyLeu: 6.974 ± 0.082	2.344GlyMet: 2.344 ± 0.047	2.814GlyAsn: 2.814 ± 0.044	1.999GlyPro: 1.999 ± 0.043	2.231GlyGln: 2.231 ± 0.052	2.94GlyArg: 2.94 ± 0.057	4.137GlySer: 4.137 ± 0.061	4.16GlyThr: 4.16 ± 0.066	5.221GlyVal: 5.221 ± 0.066	0.874GlyTrp: 0.874 ± 0.033	2.814GlyTyr: 2.814 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.304HisAla: 1.304 ± 0.033	0.21HisCys: 0.21 ± 0.014	1.023HisAsp: 1.023 ± 0.03	1.357HisGlu: 1.357 ± 0.029	1.024HisPhe: 1.024 ± 0.03	1.431HisGly: 1.431 ± 0.038	0.604HisHis: 0.604 ± 0.031	1.439HisIle: 1.439 ± 0.037	0.996HisLys: 0.996 ± 0.028	1.996HisLeu: 1.996 ± 0.045	0.612HisMet: 0.612 ± 0.02	0.672HisAsn: 0.672 ± 0.027	1.215HisPro: 1.215 ± 0.036	0.72HisGln: 0.72 ± 0.023	0.88HisArg: 0.88 ± 0.032	1.197HisSer: 1.197 ± 0.033	0.989HisThr: 0.989 ± 0.032	1.392HisVal: 1.392 ± 0.041	0.223HisTrp: 0.223 ± 0.013	0.81HisTyr: 0.81 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.018IleAla: 6.018 ± 0.083	0.646IleCys: 0.646 ± 0.023	4.333IleAsp: 4.333 ± 0.069	5.652IleGlu: 5.652 ± 0.087	3.527IlePhe: 3.527 ± 0.071	6.216IleGly: 6.216 ± 0.102	1.654IleHis: 1.654 ± 0.04	6.191IleIle: 6.191 ± 0.095	4.524IleLys: 4.524 ± 0.066	7.002IleLeu: 7.002 ± 0.09	1.861IleMet: 1.861 ± 0.043	3.206IleAsn: 3.206 ± 0.057	3.482IlePro: 3.482 ± 0.062	2.717IleGln: 2.717 ± 0.053	3.314IleArg: 3.314 ± 0.059	4.977IleSer: 4.977 ± 0.062	4.217IleThr: 4.217 ± 0.062	5.553IleVal: 5.553 ± 0.074	0.636IleTrp: 0.636 ± 0.025	2.371IleTyr: 2.371 ± 0.041	0.0IleXaa: 0.0 ± 0.0
Lys	4.909LysAla: 4.909 ± 0.077	0.298LysCys: 0.298 ± 0.018	3.961LysAsp: 3.961 ± 0.073	6.925LysGlu: 6.925 ± 0.086	2.054LysPhe: 2.054 ± 0.04	4.516LysGly: 4.516 ± 0.064	1.233LysHis: 1.233 ± 0.035	4.595LysIle: 4.595 ± 0.065	5.958LysLys: 5.958 ± 0.089	5.734LysLeu: 5.734 ± 0.086	2.204LysMet: 2.204 ± 0.039	3.297LysAsn: 3.297 ± 0.055	2.24LysPro: 2.24 ± 0.041	2.643LysGln: 2.643 ± 0.06	3.359LysArg: 3.359 ± 0.054	3.527LysSer: 3.527 ± 0.062	3.583LysThr: 3.583 ± 0.06	4.822LysVal: 4.822 ± 0.076	0.745LysTrp: 0.745 ± 0.024	2.226LysTyr: 2.226 ± 0.049	0.0LysXaa: 0.0 ± 0.0
Leu	7.493LeuAla: 7.493 ± 0.093	0.653LeuCys: 0.653 ± 0.022	4.899LeuAsp: 4.899 ± 0.066	6.708LeuGlu: 6.708 ± 0.081	4.84LeuPhe: 4.84 ± 0.079	6.666LeuGly: 6.666 ± 0.09	1.837LeuHis: 1.837 ± 0.04	7.399LeuIle: 7.399 ± 0.093	6.616LeuLys: 6.616 ± 0.072	9.991LeuLeu: 9.991 ± 0.126	2.659LeuMet: 2.659 ± 0.051	4.087LeuAsn: 4.087 ± 0.058	4.011LeuPro: 4.011 ± 0.057	3.311LeuGln: 3.311 ± 0.048	3.636LeuArg: 3.636 ± 0.06	6.484LeuSer: 6.484 ± 0.081	5.242LeuThr: 5.242 ± 0.071	6.155LeuVal: 6.155 ± 0.075	0.797LeuTrp: 0.797 ± 0.025	3.042LeuTyr: 3.042 ± 0.064	0.0LeuXaa: 0.0 ± 0.0
Met	2.279MetAla: 2.279 ± 0.043	0.154MetCys: 0.154 ± 0.011	1.567MetAsp: 1.567 ± 0.035	2.305MetGlu: 2.305 ± 0.043	1.142MetPhe: 1.142 ± 0.031	2.069MetGly: 2.069 ± 0.049	0.449MetHis: 0.449 ± 0.021	2.198MetIle: 2.198 ± 0.048	2.435MetLys: 2.435 ± 0.046	2.651MetLeu: 2.651 ± 0.054	0.903MetMet: 0.903 ± 0.031	1.492MetAsn: 1.492 ± 0.034	1.145MetPro: 1.145 ± 0.035	0.889MetGln: 0.889 ± 0.027	1.203MetArg: 1.203 ± 0.031	1.701MetSer: 1.701 ± 0.037	1.698MetThr: 1.698 ± 0.043	1.79MetVal: 1.79 ± 0.04	0.198MetTrp: 0.198 ± 0.011	0.751MetTyr: 0.751 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.717AsnAla: 2.717 ± 0.054	0.305AsnCys: 0.305 ± 0.018	2.108AsnAsp: 2.108 ± 0.048	3.359AsnGlu: 3.359 ± 0.062	1.598AsnPhe: 1.598 ± 0.04	3.092AsnGly: 3.092 ± 0.06	0.972AsnHis: 0.972 ± 0.03	3.444AsnIle: 3.444 ± 0.05	2.754AsnLys: 2.754 ± 0.046	3.65AsnLeu: 3.65 ± 0.048	1.296AsnMet: 1.296 ± 0.032	1.813AsnAsn: 1.813 ± 0.044	2.112AsnPro: 2.112 ± 0.048	1.547AsnGln: 1.547 ± 0.04	2.051AsnArg: 2.051 ± 0.042	2.125AsnSer: 2.125 ± 0.042	1.992AsnThr: 1.992 ± 0.047	2.826AsnVal: 2.826 ± 0.047	0.497AsnTrp: 0.497 ± 0.02	1.328AsnTyr: 1.328 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	2.676ProAla: 2.676 ± 0.054	0.221ProCys: 0.221 ± 0.014	2.282ProAsp: 2.282 ± 0.05	3.34ProGlu: 3.34 ± 0.055	2.011ProPhe: 2.011 ± 0.05	2.8ProGly: 2.8 ± 0.052	0.798ProHis: 0.798 ± 0.023	2.843ProIle: 2.843 ± 0.053	2.187ProLys: 2.187 ± 0.043	3.675ProLeu: 3.675 ± 0.068	0.908ProMet: 0.908 ± 0.028	1.564ProAsn: 1.564 ± 0.035	1.234ProPro: 1.234 ± 0.041	1.151ProGln: 1.151 ± 0.038	1.246ProArg: 1.246 ± 0.034	2.3ProSer: 2.3 ± 0.047	1.786ProThr: 1.786 ± 0.041	3.176ProVal: 3.176 ± 0.056	0.383ProTrp: 0.383 ± 0.019	1.392ProTyr: 1.392 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	2.636GlnAla: 2.636 ± 0.048	0.194GlnCys: 0.194 ± 0.015	1.439GlnAsp: 1.439 ± 0.037	2.702GlnGlu: 2.702 ± 0.059	1.555GlnPhe: 1.555 ± 0.033	2.122GlnGly: 2.122 ± 0.043	0.618GlnHis: 0.618 ± 0.023	2.376GlnIle: 2.376 ± 0.051	2.518GlnLys: 2.518 ± 0.049	3.638GlnLeu: 3.638 ± 0.062	1.196GlnMet: 1.196 ± 0.035	1.377GlnAsn: 1.377 ± 0.041	1.26GlnPro: 1.26 ± 0.039	1.491GlnGln: 1.491 ± 0.047	1.449GlnArg: 1.449 ± 0.036	1.963GlnSer: 1.963 ± 0.044	1.841GlnThr: 1.841 ± 0.038	2.182GlnVal: 2.182 ± 0.047	0.321GlnTrp: 0.321 ± 0.018	1.155GlnTyr: 1.155 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.722ArgAla: 2.722 ± 0.047	0.318ArgCys: 0.318 ± 0.018	2.139ArgAsp: 2.139 ± 0.047	3.443ArgGlu: 3.443 ± 0.058	1.914ArgPhe: 1.914 ± 0.041	2.555ArgGly: 2.555 ± 0.048	0.849ArgHis: 0.849 ± 0.028	3.01ArgIle: 3.01 ± 0.053	3.407ArgLys: 3.407 ± 0.062	4.237ArgLeu: 4.237 ± 0.061	1.422ArgMet: 1.422 ± 0.033	1.906ArgAsn: 1.906 ± 0.038	1.527ArgPro: 1.527 ± 0.043	1.53ArgGln: 1.53 ± 0.036	2.127ArgArg: 2.127 ± 0.044	2.356ArgSer: 2.356 ± 0.052	2.137ArgThr: 2.137 ± 0.042	2.731ArgVal: 2.731 ± 0.047	0.44ArgTrp: 0.44 ± 0.017	1.47ArgTyr: 1.47 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	4.077SerAla: 4.077 ± 0.069	0.406SerCys: 0.406 ± 0.018	2.938SerAsp: 2.938 ± 0.051	3.874SerGlu: 3.874 ± 0.052	3.169SerPhe: 3.169 ± 0.056	4.785SerGly: 4.785 ± 0.069	1.216SerHis: 1.216 ± 0.033	4.849SerIle: 4.849 ± 0.066	3.651SerLys: 3.651 ± 0.053	6.103SerLeu: 6.103 ± 0.068	1.774SerMet: 1.774 ± 0.041	2.305SerAsn: 2.305 ± 0.043	2.193SerPro: 2.193 ± 0.045	1.792SerGln: 1.792 ± 0.036	2.508SerArg: 2.508 ± 0.042	3.772SerSer: 3.772 ± 0.069	2.949SerThr: 2.949 ± 0.049	4.197SerVal: 4.197 ± 0.052	0.597SerTrp: 0.597 ± 0.025	2.083SerTyr: 2.083 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	4.288ThrAla: 4.288 ± 0.067	0.353ThrCys: 0.353 ± 0.018	2.768ThrAsp: 2.768 ± 0.05	3.415ThrGlu: 3.415 ± 0.057	2.518ThrPhe: 2.518 ± 0.052	4.468ThrGly: 4.468 ± 0.065	0.975ThrHis: 0.975 ± 0.026	4.303ThrIle: 4.303 ± 0.065	3.167ThrLys: 3.167 ± 0.061	4.793ThrLeu: 4.793 ± 0.065	1.391ThrMet: 1.391 ± 0.034	2.066ThrAsn: 2.066 ± 0.048	2.287ThrPro: 2.287 ± 0.042	1.229ThrGln: 1.229 ± 0.03	1.821ThrArg: 1.821 ± 0.044	3.103ThrSer: 3.103 ± 0.055	2.669ThrThr: 2.669 ± 0.046	4.314ThrVal: 4.314 ± 0.063	0.535ThrTrp: 0.535 ± 0.024	1.773ThrTyr: 1.773 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	4.986ValAla: 4.986 ± 0.082	0.584ValCys: 0.584 ± 0.023	3.729ValAsp: 3.729 ± 0.062	4.879ValGlu: 4.879 ± 0.073	3.2ValPhe: 3.2 ± 0.053	4.706ValGly: 4.706 ± 0.071	1.34ValHis: 1.34 ± 0.034	5.948ValIle: 5.948 ± 0.079	4.707ValLys: 4.707 ± 0.067	6.893ValLeu: 6.893 ± 0.082	1.931ValMet: 1.931 ± 0.04	3.125ValAsn: 3.125 ± 0.057	2.89ValPro: 2.89 ± 0.047	2.295ValGln: 2.295 ± 0.042	2.804ValArg: 2.804 ± 0.049	4.458ValSer: 4.458 ± 0.068	4.094ValThr: 4.094 ± 0.064	4.949ValVal: 4.949 ± 0.082	0.653ValTrp: 0.653 ± 0.025	2.128ValTyr: 2.128 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.641TrpAla: 0.641 ± 0.027	0.075TrpCys: 0.075 ± 0.009	0.499TrpAsp: 0.499 ± 0.02	0.691TrpGlu: 0.691 ± 0.025	0.481TrpPhe: 0.481 ± 0.023	0.711TrpGly: 0.711 ± 0.032	0.188TrpHis: 0.188 ± 0.011	0.821TrpIle: 0.821 ± 0.029	0.784TrpLys: 0.784 ± 0.031	1.055TrpLeu: 1.055 ± 0.036	0.323TrpMet: 0.323 ± 0.016	0.508TrpAsn: 0.508 ± 0.02	0.266TrpPro: 0.266 ± 0.015	0.322TrpGln: 0.322 ± 0.015	0.417TrpArg: 0.417 ± 0.021	0.59TrpSer: 0.59 ± 0.021	0.54TrpThr: 0.54 ± 0.023	0.677TrpVal: 0.677 ± 0.028	0.128TrpTrp: 0.128 ± 0.011	0.333TrpTyr: 0.333 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.094TyrAla: 2.094 ± 0.042	0.273TyrCys: 0.273 ± 0.018	1.935TyrAsp: 1.935 ± 0.041	2.479TyrGlu: 2.479 ± 0.053	1.732TyrPhe: 1.732 ± 0.043	2.484TyrGly: 2.484 ± 0.046	0.792TyrHis: 0.792 ± 0.026	2.384TyrIle: 2.384 ± 0.043	2.01TyrLys: 2.01 ± 0.045	3.19TyrLeu: 3.19 ± 0.051	0.854TyrMet: 0.854 ± 0.023	1.321TyrAsn: 1.321 ± 0.035	1.364TyrPro: 1.364 ± 0.039	1.241TyrGln: 1.241 ± 0.034	1.615TyrArg: 1.615 ± 0.041	2.112TyrSer: 2.112 ± 0.043	1.758TyrThr: 1.758 ± 0.038	2.087TyrVal: 2.087 ± 0.046	0.373TyrTrp: 0.373 ± 0.019	1.309TyrTyr: 1.309 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3998 proteins (1178504 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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