Pistachio ampelovirus A

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Ampelovirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A499PLS0|A0A499PLS0_9CLOS Uncharacterized protein OS=Pistachio ampelovirus A OX=2093224 PE=4 SV=1
MM1 pKa = 7.56INCFLEE7 pKa = 4.61DD8 pKa = 3.48VVAGSTSLEE17 pKa = 4.02FFHH20 pKa = 8.52RR21 pKa = 11.84EE22 pKa = 3.69DD23 pKa = 3.54CVEE26 pKa = 4.85FEE28 pKa = 5.71DD29 pKa = 6.08FDD31 pKa = 6.28ANLDD35 pKa = 3.62NVVIRR40 pKa = 11.84EE41 pKa = 4.38SEE43 pKa = 4.1NKK45 pKa = 10.27APTRR49 pKa = 11.84NDD51 pKa = 3.19PTTVPVIRR59 pKa = 11.84SQIGSKK65 pKa = 9.99KK66 pKa = 10.32RR67 pKa = 11.84NTLRR71 pKa = 11.84SNLLTFEE78 pKa = 4.33ARR80 pKa = 11.84NFNADD85 pKa = 3.26RR86 pKa = 11.84GVDD89 pKa = 3.72ISCSDD94 pKa = 3.74VVSDD98 pKa = 4.03ILVDD102 pKa = 3.75NFFSRR107 pKa = 11.84CIDD110 pKa = 3.46SSKK113 pKa = 10.97LEE115 pKa = 3.95QANTEE120 pKa = 4.42SINVNLRR127 pKa = 11.84VISDD131 pKa = 3.55WLSSRR136 pKa = 11.84TGTGYY141 pKa = 10.74GALVGEE147 pKa = 4.71LQRR150 pKa = 11.84PSDD153 pKa = 3.88VADD156 pKa = 3.63GLTNFKK162 pKa = 10.75IMVKK166 pKa = 10.18SDD168 pKa = 3.7AKK170 pKa = 10.59PKK172 pKa = 10.06LDD174 pKa = 3.52EE175 pKa = 4.81SIISKK180 pKa = 10.46VSTGQNIVYY189 pKa = 10.07HH190 pKa = 5.84KK191 pKa = 10.39RR192 pKa = 11.84KK193 pKa = 9.79INAIFSNIFLQVVEE207 pKa = 4.3RR208 pKa = 11.84LKK210 pKa = 10.93FALAHH215 pKa = 6.19NIVLYY220 pKa = 10.46TGMNTDD226 pKa = 3.31DD227 pKa = 4.18FARR230 pKa = 11.84EE231 pKa = 3.79VEE233 pKa = 4.36DD234 pKa = 6.49RR235 pKa = 11.84IGCDD239 pKa = 2.67INSFFCSEE247 pKa = 4.25LDD249 pKa = 2.91ISKK252 pKa = 10.56YY253 pKa = 10.61DD254 pKa = 3.49KK255 pKa = 11.33SQGALFKK262 pKa = 10.74QVEE265 pKa = 4.41EE266 pKa = 4.84KK267 pKa = 10.46ILARR271 pKa = 11.84LGVSPGVLDD280 pKa = 3.52LWYY283 pKa = 10.02ASEE286 pKa = 4.47YY287 pKa = 9.6EE288 pKa = 4.37SCVTSADD295 pKa = 3.61RR296 pKa = 11.84TFSGDD301 pKa = 2.14IGAQRR306 pKa = 11.84RR307 pKa = 11.84SGASNTWLGNTLINMCLQSYY327 pKa = 7.96CTDD330 pKa = 3.98FSDD333 pKa = 5.45FPCICFAGDD342 pKa = 3.44DD343 pKa = 3.79SLIFSRR349 pKa = 11.84SPLANVYY356 pKa = 11.04GDD358 pKa = 3.41IQTHH362 pKa = 6.45FGFDD366 pKa = 2.98MKK368 pKa = 10.51FYY370 pKa = 10.44EE371 pKa = 4.05RR372 pKa = 11.84AVPYY376 pKa = 9.95FCSKK380 pKa = 10.92YY381 pKa = 10.39LVSDD385 pKa = 4.17GSKK388 pKa = 10.7LFFVPDD394 pKa = 4.12PYY396 pKa = 11.17KK397 pKa = 11.05VLVKK401 pKa = 10.57LGRR404 pKa = 11.84LTSSKK409 pKa = 10.84EE410 pKa = 4.21SLCHH414 pKa = 6.77EE415 pKa = 4.61NFQSLCDD422 pKa = 3.72SVKK425 pKa = 10.34YY426 pKa = 10.82LNNEE430 pKa = 4.06TIINMLVFYY439 pKa = 7.39HH440 pKa = 6.13HH441 pKa = 7.14CKK443 pKa = 10.48YY444 pKa = 11.02GPTNYY449 pKa = 9.81TYY451 pKa = 11.19GAFASIHH458 pKa = 6.71CITANFSQFRR468 pKa = 11.84RR469 pKa = 11.84LFIRR473 pKa = 11.84RR474 pKa = 11.84NAKK477 pKa = 10.3SFGFDD482 pKa = 4.07LNDD485 pKa = 3.22SS486 pKa = 3.83

Molecular weight: 54.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A499Q1W1|A0A499Q1W1_9CLOS Uncharacterized protein OS=Pistachio ampelovirus A OX=2093224 PE=4 SV=1
MM1 pKa = 7.24SLKK4 pKa = 10.78HH5 pKa = 6.01MGNIISDD12 pKa = 3.76YY13 pKa = 10.54EE14 pKa = 4.0RR15 pKa = 11.84DD16 pKa = 3.69YY17 pKa = 11.94LEE19 pKa = 4.2LNRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84GNYY27 pKa = 9.84GEE29 pKa = 3.99PTIVNYY35 pKa = 10.76NGADD39 pKa = 3.3YY40 pKa = 11.11GGYY43 pKa = 9.84RR44 pKa = 11.84RR45 pKa = 11.84LDD47 pKa = 3.09SSNYY51 pKa = 9.33HH52 pKa = 6.12NYY54 pKa = 10.81DD55 pKa = 3.03YY56 pKa = 10.99FDD58 pKa = 3.83PRR60 pKa = 11.84RR61 pKa = 11.84EE62 pKa = 3.88RR63 pKa = 11.84DD64 pKa = 3.52NKK66 pKa = 10.6LLEE69 pKa = 4.01VWNDD73 pKa = 3.05HH74 pKa = 5.32ATEE77 pKa = 4.53LSQMKK82 pKa = 8.92TRR84 pKa = 11.84EE85 pKa = 3.56TRR87 pKa = 11.84YY88 pKa = 9.36EE89 pKa = 3.6YY90 pKa = 10.35EE91 pKa = 3.68RR92 pKa = 11.84RR93 pKa = 11.84MNIINEE99 pKa = 3.93YY100 pKa = 10.68DD101 pKa = 3.65LEE103 pKa = 4.35LKK105 pKa = 10.24KK106 pKa = 10.74LKK108 pKa = 10.3KK109 pKa = 9.63EE110 pKa = 4.07HH111 pKa = 6.1EE112 pKa = 4.39EE113 pKa = 4.07KK114 pKa = 10.8LKK116 pKa = 10.75KK117 pKa = 10.62AYY119 pKa = 9.48DD120 pKa = 3.2KK121 pKa = 11.03HH122 pKa = 8.05AEE124 pKa = 4.05RR125 pKa = 11.84KK126 pKa = 8.94RR127 pKa = 11.84KK128 pKa = 9.91SFIYY132 pKa = 10.17RR133 pKa = 11.84NLYY136 pKa = 9.17RR137 pKa = 5.6

Molecular weight: 17.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5271

137

1446

439.3

49.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.824 ± 0.39

1.954 ± 0.407

6.109 ± 0.35

6.488 ± 0.651

4.534 ± 0.463

5.559 ± 0.509

1.707 ± 0.179

6.109 ± 0.588

6.242 ± 0.783

9.732 ± 0.392

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.878 ± 0.192

5.255 ± 0.391

2.903 ± 0.38

2.22 ± 0.214

5.919 ± 0.34

8.329 ± 0.403

6.014 ± 0.637

8.272 ± 0.546

0.74 ± 0.135

4.212 ± 0.444
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski