Pochonia chlamydosporia 170

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Hypocreales; Clavicipitaceae; Pochonia; Pochonia chlamydosporia

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14143 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A179FX06|A0A179FX06_METCM Sin3 binding protein OS=Pochonia chlamydosporia 170 OX=1380566 GN=VFPPC_13311 PE=4 SV=1
MM1 pKa = 7.24HH2 pKa = 7.89RR3 pKa = 11.84ATISAAVATLLAGNVLARR21 pKa = 11.84DD22 pKa = 3.68VPSNVKK28 pKa = 10.15ALYY31 pKa = 10.36DD32 pKa = 3.94SIRR35 pKa = 11.84SSGSCSNVLQGGFFSQEE52 pKa = 3.69DD53 pKa = 3.78DD54 pKa = 4.11SKK56 pKa = 11.67DD57 pKa = 3.17FCYY60 pKa = 10.75CGDD63 pKa = 3.76HH64 pKa = 6.55LQDD67 pKa = 3.67KK68 pKa = 10.55GIIYY72 pKa = 9.64LQGNGGQLVNMDD84 pKa = 4.35IDD86 pKa = 4.43CDD88 pKa = 3.62GHH90 pKa = 8.32LGQGNGDD97 pKa = 4.06CDD99 pKa = 4.56SSGDD103 pKa = 3.85TQGQTTFGDD112 pKa = 4.07TVASYY117 pKa = 11.52DD118 pKa = 4.96KK119 pKa = 11.51GIDD122 pKa = 3.48DD123 pKa = 4.54LNAYY127 pKa = 7.09VHH129 pKa = 6.54SYY131 pKa = 10.43VVLGNQGSGDD141 pKa = 3.85GYY143 pKa = 11.22VEE145 pKa = 4.63YY146 pKa = 10.41DD147 pKa = 3.48PQGDD151 pKa = 3.88GVEE154 pKa = 4.2PLSVVAVVCGDD165 pKa = 3.01KK166 pKa = 10.35MFYY169 pKa = 10.56GVWGDD174 pKa = 3.62TNGDD178 pKa = 3.78DD179 pKa = 4.39GPPLVGEE186 pKa = 4.42VSLSLGQACYY196 pKa = 10.68GRR198 pKa = 11.84AVNGNEE204 pKa = 3.66AHH206 pKa = 7.61DD207 pKa = 5.13DD208 pKa = 3.72NDD210 pKa = 3.28VLYY213 pKa = 10.38IAFKK217 pKa = 10.7GSNAVPGADD226 pKa = 3.38GADD229 pKa = 3.15WGASSFDD236 pKa = 3.69DD237 pKa = 4.57FEE239 pKa = 6.15NSLASLGDD247 pKa = 3.74SLVSQLL253 pKa = 4.58

Molecular weight: 26.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A219ARW6|A0A219ARW6_METCM Uncharacterized protein OS=Pochonia chlamydosporia 170 OX=1380566 GN=VFPPC_17354 PE=4 SV=1
MM1 pKa = 7.51HH2 pKa = 7.43SLNFSLIIHH11 pKa = 5.72VQGTWILLKK20 pKa = 10.54RR21 pKa = 11.84QVATTGRR28 pKa = 11.84RR29 pKa = 11.84HH30 pKa = 5.88HH31 pKa = 6.29HH32 pKa = 6.12HH33 pKa = 6.26QLNSSNRR40 pKa = 11.84SRR42 pKa = 11.84KK43 pKa = 6.85TQALLTPRR51 pKa = 11.84TT52 pKa = 3.82

Molecular weight: 6.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14143

0

14143

6505054

38

9980

459.9

50.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.52 ± 0.02

1.395 ± 0.009

5.762 ± 0.016

5.847 ± 0.02

3.738 ± 0.013

6.931 ± 0.023

2.452 ± 0.009

4.968 ± 0.015

4.936 ± 0.019

8.921 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.275 ± 0.008

3.791 ± 0.01

5.734 ± 0.022

4.083 ± 0.015

6.067 ± 0.022

8.086 ± 0.019

5.966 ± 0.015

6.208 ± 0.017

1.567 ± 0.007

2.752 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski