candidate division MSBL1 archaeon SCGC-AAA833F18

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 278 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133VSB8|A0A133VSB8_9EURY ORC1-type DNA replication protein OS=candidate division MSBL1 archaeon SCGC-AAA833F18 OX=1698257 GN=AKJ35_00990 PE=3 SV=1
MM1 pKa = 7.0VAEE4 pKa = 5.19DD5 pKa = 3.83YY6 pKa = 9.27EE7 pKa = 4.71IPEE10 pKa = 3.98GLYY13 pKa = 10.51YY14 pKa = 10.32SEE16 pKa = 3.7EE17 pKa = 4.35HH18 pKa = 6.06EE19 pKa = 4.41WVKK22 pKa = 11.06VEE24 pKa = 4.96DD25 pKa = 3.41NLARR29 pKa = 11.84VGITDD34 pKa = 3.58YY35 pKa = 11.59AQDD38 pKa = 3.41QLGDD42 pKa = 3.6VVYY45 pKa = 11.11ADD47 pKa = 4.55LPEE50 pKa = 5.73PGDD53 pKa = 3.62KK54 pKa = 10.7VKK56 pKa = 9.31QTTEE60 pKa = 3.91EE61 pKa = 4.12KK62 pKa = 10.41SEE64 pKa = 3.83EE65 pKa = 4.13MEE67 pKa = 4.3LGAVEE72 pKa = 4.52SIKK75 pKa = 10.8AVSSVYY81 pKa = 10.61SPLSGEE87 pKa = 4.18VKK89 pKa = 10.14EE90 pKa = 4.73VNDD93 pKa = 4.47DD94 pKa = 5.24LEE96 pKa = 4.57DD97 pKa = 3.69QPEE100 pKa = 4.27LVNTDD105 pKa = 4.04PYY107 pKa = 9.37TGGWICVIDD116 pKa = 4.49PEE118 pKa = 5.1DD119 pKa = 4.57LDD121 pKa = 4.93SEE123 pKa = 4.92LEE125 pKa = 3.96NLMDD129 pKa = 4.45AKK131 pKa = 10.83AYY133 pKa = 10.58ADD135 pKa = 3.55FLEE138 pKa = 4.7SEE140 pKa = 4.57EE141 pKa = 4.17

Molecular weight: 15.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VT42|A0A133VT42_9EURY Uncharacterized protein OS=candidate division MSBL1 archaeon SCGC-AAA833F18 OX=1698257 GN=AKJ35_00440 PE=4 SV=1
MM1 pKa = 7.25TKK3 pKa = 10.2EE4 pKa = 4.0SQEE7 pKa = 3.72VSVNLRR13 pKa = 11.84RR14 pKa = 11.84TLIPIFIVTAIIIIAVVLLLQEE36 pKa = 4.51NIGGIVTALQNANYY50 pKa = 10.35LFILLAFIIYY60 pKa = 9.98FSSAFFWAARR70 pKa = 11.84WRR72 pKa = 11.84IGLFALGKK80 pKa = 8.37NASMRR85 pKa = 11.84NLFPIIWGSMFINNMTPLNRR105 pKa = 11.84AGGDD109 pKa = 3.73PIGRR113 pKa = 11.84PYY115 pKa = 11.04LLKK118 pKa = 9.25KK119 pKa = 9.02TSGIRR124 pKa = 11.84FRR126 pKa = 11.84MGFASVVAEE135 pKa = 4.06QLFQIPVVLFLLAMGLILRR154 pKa = 11.84VQMTANPGLVSIATVVISLSLAIVLVPFFILLFGKK189 pKa = 10.37KK190 pKa = 8.65EE191 pKa = 3.94FSSKK195 pKa = 8.66ITGFLTKK202 pKa = 9.61ILKK205 pKa = 9.5FFRR208 pKa = 11.84RR209 pKa = 11.84EE210 pKa = 3.63ADD212 pKa = 3.06PTKK215 pKa = 10.32ILKK218 pKa = 8.66TVEE221 pKa = 4.1DD222 pKa = 4.35FRR224 pKa = 11.84VSARR228 pKa = 11.84KK229 pKa = 9.46IINSKK234 pKa = 10.5HH235 pKa = 4.46NAAWMMGFSAALWILDD251 pKa = 3.68LVRR254 pKa = 11.84ISLILYY260 pKa = 9.67ALGINISLPMLFLASTLPQIAGLVPLLPGGIGAVEE295 pKa = 3.97VTMVSVFMWFGVSPVLALSATLIEE319 pKa = 4.41RR320 pKa = 11.84SMSFVFSTLIGAGALSYY337 pKa = 10.92LGIKK341 pKa = 9.46IWTKK345 pKa = 10.1SS346 pKa = 3.15

Molecular weight: 38.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

278

0

278

75280

62

1062

270.8

30.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.256 ± 0.153

0.893 ± 0.049

5.514 ± 0.103

9.327 ± 0.191

3.544 ± 0.104

7.54 ± 0.139

1.85 ± 0.053

7.184 ± 0.12

6.983 ± 0.144

9.68 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.061

3.334 ± 0.106

4.332 ± 0.084

2.582 ± 0.082

5.498 ± 0.123

6.095 ± 0.135

5.096 ± 0.129

7.139 ± 0.116

1.089 ± 0.058

2.82 ± 0.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski