Amino acid dipepetide frequency for Ruegeria phage vB_RpoMi-V15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.281AlaAla: 9.281 ± 2.39
0.773AlaCys: 0.773 ± 0.544
6.187AlaAsp: 6.187 ± 2.353
3.094AlaGlu: 3.094 ± 1.473
4.64AlaPhe: 4.64 ± 1.732
8.507AlaGly: 8.507 ± 2.006
2.32AlaHis: 2.32 ± 0.316
3.094AlaIle: 3.094 ± 0.932
4.64AlaLys: 4.64 ± 1.813
6.961AlaLeu: 6.961 ± 2.11
3.094AlaMet: 3.094 ± 1.92
3.094AlaAsn: 3.094 ± 1.611
5.414AlaPro: 5.414 ± 1.506
2.32AlaGln: 2.32 ± 0.906
13.148AlaArg: 13.148 ± 4.319
6.961AlaSer: 6.961 ± 1.557
3.094AlaThr: 3.094 ± 0.538
3.867AlaVal: 3.867 ± 0.743
1.547AlaTrp: 1.547 ± 1.232
3.094AlaTyr: 3.094 ± 1.103
0.0AlaXaa: 0.0 ± 0.0
Cys
0.773CysAla: 0.773 ± 0.858
0.0CysCys: 0.0 ± 0.0
0.773CysAsp: 0.773 ± 0.544
0.0CysGlu: 0.0 ± 0.0
0.0CysPhe: 0.0 ± 0.0
1.547CysGly: 1.547 ± 1.716
0.773CysHis: 0.773 ± 0.544
0.0CysIle: 0.0 ± 0.0
2.32CysLys: 2.32 ± 1.314
0.0CysLeu: 0.0 ± 0.0
0.773CysMet: 0.773 ± 0.544
0.773CysAsn: 0.773 ± 0.616
1.547CysPro: 1.547 ± 1.089
0.0CysGln: 0.0 ± 0.0
0.773CysArg: 0.773 ± 0.544
0.773CysSer: 0.773 ± 0.544
1.547CysThr: 1.547 ± 1.232
0.0CysVal: 0.0 ± 0.0
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
8.507AspAla: 8.507 ± 0.98
0.0AspCys: 0.0 ± 0.0
0.773AspAsp: 0.773 ± 0.616
1.547AspGlu: 1.547 ± 0.71
0.773AspPhe: 0.773 ± 0.544
5.414AspGly: 5.414 ± 1.632
2.32AspHis: 2.32 ± 0.826
3.094AspIle: 3.094 ± 1.442
0.773AspLys: 0.773 ± 0.544
5.414AspLeu: 5.414 ± 2.47
0.773AspMet: 0.773 ± 0.616
3.094AspAsn: 3.094 ± 1.391
5.414AspPro: 5.414 ± 2.154
2.32AspGln: 2.32 ± 1.131
3.094AspArg: 3.094 ± 2.178
0.773AspSer: 0.773 ± 0.697
0.773AspThr: 0.773 ± 0.544
3.094AspVal: 3.094 ± 0.932
3.094AspTrp: 3.094 ± 1.09
1.547AspTyr: 1.547 ± 0.71
0.0AspXaa: 0.0 ± 0.0
Glu
2.32GluAla: 2.32 ± 0.906
1.547GluCys: 1.547 ± 1.089
1.547GluAsp: 1.547 ± 0.71
1.547GluGlu: 1.547 ± 0.551
3.094GluPhe: 3.094 ± 1.669
4.64GluGly: 4.64 ± 2.65
1.547GluHis: 1.547 ± 0.674
3.094GluIle: 3.094 ± 1.92
3.867GluLys: 3.867 ± 1.907
3.867GluLeu: 3.867 ± 1.785
2.32GluMet: 2.32 ± 1.035
0.0GluAsn: 0.0 ± 0.0
1.547GluPro: 1.547 ± 1.232
0.773GluGln: 0.773 ± 0.616
6.187GluArg: 6.187 ± 2.619
0.773GluSer: 0.773 ± 0.616
3.867GluThr: 3.867 ± 2.209
2.32GluVal: 2.32 ± 1.008
0.0GluTrp: 0.0 ± 0.0
2.32GluTyr: 2.32 ± 0.316
0.0GluXaa: 0.0 ± 0.0
Phe
0.773PheAla: 0.773 ± 0.616
1.547PheCys: 1.547 ± 0.551
1.547PheAsp: 1.547 ± 1.089
0.773PheGlu: 0.773 ± 0.616
3.094PhePhe: 3.094 ± 1.391
4.64PheGly: 4.64 ± 0.856
1.547PheHis: 1.547 ± 0.674
3.094PheIle: 3.094 ± 2.464
1.547PheLys: 1.547 ± 0.71
0.0PheLeu: 0.0 ± 0.0
2.32PheMet: 2.32 ± 1.19
2.32PheAsn: 2.32 ± 1.035
0.0PhePro: 0.0 ± 0.0
2.32PheGln: 2.32 ± 1.317
1.547PheArg: 1.547 ± 0.925
2.32PheSer: 2.32 ± 2.091
3.867PheThr: 3.867 ± 1.785
2.32PheVal: 2.32 ± 1.675
1.547PheTrp: 1.547 ± 1.073
2.32PheTyr: 2.32 ± 1.131
0.0PheXaa: 0.0 ± 0.0
Gly
9.281GlyAla: 9.281 ± 2.15
1.547GlyCys: 1.547 ± 0.967
2.32GlyAsp: 2.32 ± 0.906
4.64GlyGlu: 4.64 ± 1.055
2.32GlyPhe: 2.32 ± 1.131
14.695GlyGly: 14.695 ± 5.383
4.64GlyHis: 4.64 ± 0.521
4.64GlyIle: 4.64 ± 2.38
6.187GlyLys: 6.187 ± 1.728
4.64GlyLeu: 4.64 ± 1.087
2.32GlyMet: 2.32 ± 1.317
3.867GlyAsn: 3.867 ± 1.035
3.867GlyPro: 3.867 ± 1.051
2.32GlyGln: 2.32 ± 1.131
4.64GlyArg: 4.64 ± 0.931
5.414GlySer: 5.414 ± 2.283
3.867GlyThr: 3.867 ± 2.26
10.054GlyVal: 10.054 ± 2.821
2.32GlyTrp: 2.32 ± 1.265
1.547GlyTyr: 1.547 ± 1.089
0.0GlyXaa: 0.0 ± 0.0
His
2.32HisAla: 2.32 ± 1.314
0.0HisCys: 0.0 ± 0.0
1.547HisAsp: 1.547 ± 0.674
2.32HisGlu: 2.32 ± 0.316
0.773HisPhe: 0.773 ± 0.544
0.0HisGly: 0.0 ± 0.0
0.0HisHis: 0.0 ± 0.0
1.547HisIle: 1.547 ± 1.073
0.773HisLys: 0.773 ± 0.697
3.867HisLeu: 3.867 ± 0.568
0.0HisMet: 0.0 ± 0.0
0.773HisAsn: 0.773 ± 0.858
3.094HisPro: 3.094 ± 0.932
1.547HisGln: 1.547 ± 0.967
3.094HisArg: 3.094 ± 1.348
3.094HisSer: 3.094 ± 1.09
1.547HisThr: 1.547 ± 0.551
3.094HisVal: 3.094 ± 1.05
0.773HisTrp: 0.773 ± 0.697
2.32HisTyr: 2.32 ± 0.316
0.0HisXaa: 0.0 ± 0.0
Ile
6.961IleAla: 6.961 ± 1.783
0.0IleCys: 0.0 ± 0.0
2.32IleAsp: 2.32 ± 1.265
3.094IleGlu: 3.094 ± 2.178
0.773IlePhe: 0.773 ± 0.544
1.547IleGly: 1.547 ± 1.073
3.094IleHis: 3.094 ± 1.09
0.0IleIle: 0.0 ± 0.0
3.094IleLys: 3.094 ± 0.71
3.094IleLeu: 3.094 ± 1.419
0.773IleMet: 0.773 ± 0.616
2.32IleAsn: 2.32 ± 1.848
1.547IlePro: 1.547 ± 0.674
0.0IleGln: 0.0 ± 0.0
3.867IleArg: 3.867 ± 1.479
0.773IleSer: 0.773 ± 0.697
2.32IleThr: 2.32 ± 1.259
1.547IleVal: 1.547 ± 0.674
0.773IleTrp: 0.773 ± 0.697
2.32IleTyr: 2.32 ± 0.826
0.0IleXaa: 0.0 ± 0.0
Lys
6.961LysAla: 6.961 ± 3.445
3.094LysCys: 3.094 ± 1.05
3.867LysAsp: 3.867 ± 0.568
1.547LysGlu: 1.547 ± 1.089
0.773LysPhe: 0.773 ± 0.697
4.64LysGly: 4.64 ± 0.931
1.547LysHis: 1.547 ± 1.394
1.547LysIle: 1.547 ± 1.089
3.094LysLys: 3.094 ± 1.39
5.414LysLeu: 5.414 ± 1.074
0.773LysMet: 0.773 ± 1.122
3.094LysAsn: 3.094 ± 1.678
3.094LysPro: 3.094 ± 1.611
1.547LysGln: 1.547 ± 1.089
4.64LysArg: 4.64 ± 0.856
3.094LysSer: 3.094 ± 0.71
2.32LysThr: 2.32 ± 1.131
2.32LysVal: 2.32 ± 0.906
0.773LysTrp: 0.773 ± 0.697
3.867LysTyr: 3.867 ± 1.907
0.0LysXaa: 0.0 ± 0.0
Leu
9.281LeuAla: 9.281 ± 1.614
0.0LeuCys: 0.0 ± 0.0
3.867LeuAsp: 3.867 ± 1.051
1.547LeuGlu: 1.547 ± 0.71
3.094LeuPhe: 3.094 ± 2.464
9.281LeuGly: 9.281 ± 2.249
0.773LeuHis: 0.773 ± 0.544
2.32LeuIle: 2.32 ± 1.035
3.094LeuLys: 3.094 ± 0.71
7.734LeuLeu: 7.734 ± 2.084
0.773LeuMet: 0.773 ± 0.616
3.094LeuAsn: 3.094 ± 2.464
7.734LeuPro: 7.734 ± 1.592
3.094LeuGln: 3.094 ± 1.194
4.64LeuArg: 4.64 ± 1.715
3.867LeuSer: 3.867 ± 1.454
2.32LeuThr: 2.32 ± 1.633
5.414LeuVal: 5.414 ± 0.896
0.773LeuTrp: 0.773 ± 0.544
3.867LeuTyr: 3.867 ± 0.568
0.0LeuXaa: 0.0 ± 0.0
Met
1.547MetAla: 1.547 ± 0.674
0.0MetCys: 0.0 ± 0.0
1.547MetAsp: 1.547 ± 0.967
1.547MetGlu: 1.547 ± 1.073
2.32MetPhe: 2.32 ± 1.848
1.547MetGly: 1.547 ± 0.551
0.0MetHis: 0.0 ± 0.0
0.773MetIle: 0.773 ± 0.544
0.773MetLys: 0.773 ± 0.616
2.32MetLeu: 2.32 ± 0.953
0.0MetMet: 0.0 ± 0.0
0.0MetAsn: 0.0 ± 0.0
0.773MetPro: 0.773 ± 0.697
2.32MetGln: 2.32 ± 1.848
2.32MetArg: 2.32 ± 1.008
0.0MetSer: 0.0 ± 0.0
2.32MetThr: 2.32 ± 0.316
0.773MetVal: 0.773 ± 0.616
0.773MetTrp: 0.773 ± 0.616
1.547MetTyr: 1.547 ± 0.71
0.0MetXaa: 0.0 ± 0.0
Asn
3.867AsnAla: 3.867 ± 2.255
0.0AsnCys: 0.0 ± 0.0
3.867AsnAsp: 3.867 ± 2.26
2.32AsnGlu: 2.32 ± 1.131
0.0AsnPhe: 0.0 ± 0.0
5.414AsnGly: 5.414 ± 1.195
0.773AsnHis: 0.773 ± 0.697
0.773AsnIle: 0.773 ± 0.616
3.867AsnLys: 3.867 ± 0.854
3.094AsnLeu: 3.094 ± 1.194
0.773AsnMet: 0.773 ± 0.616
0.773AsnAsn: 0.773 ± 0.616
3.867AsnPro: 3.867 ± 1.539
0.773AsnGln: 0.773 ± 0.544
1.547AsnArg: 1.547 ± 0.551
3.094AsnSer: 3.094 ± 1.103
1.547AsnThr: 1.547 ± 1.232
0.773AsnVal: 0.773 ± 0.544
0.0AsnTrp: 0.0 ± 0.0
0.0AsnTyr: 0.0 ± 0.0
0.0AsnXaa: 0.0 ± 0.0
Pro
6.187ProAla: 6.187 ± 2.172
0.773ProCys: 0.773 ± 0.544
5.414ProAsp: 5.414 ± 1.506
3.867ProGlu: 3.867 ± 1.668
3.094ProPhe: 3.094 ± 0.638
6.187ProGly: 6.187 ± 1.863
0.773ProHis: 0.773 ± 0.697
1.547ProIle: 1.547 ± 1.089
2.32ProLys: 2.32 ± 0.316
5.414ProLeu: 5.414 ± 1.766
0.773ProMet: 0.773 ± 0.616
1.547ProAsn: 1.547 ± 1.394
5.414ProPro: 5.414 ± 1.493
2.32ProGln: 2.32 ± 0.953
4.64ProArg: 4.64 ± 2.022
3.094ProSer: 3.094 ± 0.638
4.64ProThr: 4.64 ± 0.521
6.187ProVal: 6.187 ± 2.291
0.0ProTrp: 0.0 ± 0.0
2.32ProTyr: 2.32 ± 0.316
0.0ProXaa: 0.0 ± 0.0
Gln
3.094GlnAla: 3.094 ± 1.473
0.773GlnCys: 0.773 ± 0.858
0.773GlnAsp: 0.773 ± 0.616
1.547GlnGlu: 1.547 ± 1.232
0.773GlnPhe: 0.773 ± 0.616
4.64GlnGly: 4.64 ± 1.654
0.773GlnHis: 0.773 ± 0.858
2.32GlnIle: 2.32 ± 1.265
0.773GlnLys: 0.773 ± 0.544
0.773GlnLeu: 0.773 ± 0.616
1.547GlnMet: 1.547 ± 0.589
2.32GlnAsn: 2.32 ± 1.035
0.773GlnPro: 0.773 ± 0.858
1.547GlnGln: 1.547 ± 1.232
2.32GlnArg: 2.32 ± 0.953
3.094GlnSer: 3.094 ± 1.218
3.094GlnThr: 3.094 ± 0.538
1.547GlnVal: 1.547 ± 0.551
0.0GlnTrp: 0.0 ± 0.0
0.0GlnTyr: 0.0 ± 0.0
0.0GlnXaa: 0.0 ± 0.0
Arg
9.281ArgAla: 9.281 ± 2.174
0.773ArgCys: 0.773 ± 0.858
3.867ArgAsp: 3.867 ± 2.6
3.867ArgGlu: 3.867 ± 2.027
2.32ArgPhe: 2.32 ± 0.316
4.64ArgGly: 4.64 ± 1.087
2.32ArgHis: 2.32 ± 1.01
0.773ArgIle: 0.773 ± 0.544
3.867ArgLys: 3.867 ± 0.568
5.414ArgLeu: 5.414 ± 3.031
0.773ArgMet: 0.773 ± 0.544
5.414ArgAsn: 5.414 ± 2.432
6.187ArgPro: 6.187 ± 2.951
2.32ArgGln: 2.32 ± 1.633
4.64ArgArg: 4.64 ± 1.715
1.547ArgSer: 1.547 ± 1.089
6.187ArgThr: 6.187 ± 0.512
4.64ArgVal: 4.64 ± 2.451
2.32ArgTrp: 2.32 ± 0.826
3.094ArgTyr: 3.094 ± 1.218
0.0ArgXaa: 0.0 ± 0.0
Ser
5.414SerAla: 5.414 ± 1.648
0.773SerCys: 0.773 ± 0.544
3.094SerAsp: 3.094 ± 1.103
6.187SerGlu: 6.187 ± 1.473
2.32SerPhe: 2.32 ± 1.131
5.414SerGly: 5.414 ± 1.481
1.547SerHis: 1.547 ± 0.551
1.547SerIle: 1.547 ± 1.089
3.867SerLys: 3.867 ± 1.291
3.867SerLeu: 3.867 ± 1.035
0.773SerMet: 0.773 ± 0.697
1.547SerAsn: 1.547 ± 1.089
2.32SerPro: 2.32 ± 1.259
1.547SerGln: 1.547 ± 0.967
4.64SerArg: 4.64 ± 3.266
3.867SerSer: 3.867 ± 1.291
1.547SerThr: 1.547 ± 0.71
0.773SerVal: 0.773 ± 0.544
0.0SerTrp: 0.0 ± 0.0
0.0SerTyr: 0.0 ± 0.0
0.0SerXaa: 0.0 ± 0.0
Thr
3.867ThrAla: 3.867 ± 1.291
0.0ThrCys: 0.0 ± 0.0
2.32ThrAsp: 2.32 ± 0.316
1.547ThrGlu: 1.547 ± 0.71
2.32ThrPhe: 2.32 ± 1.01
5.414ThrGly: 5.414 ± 1.074
0.773ThrHis: 0.773 ± 0.544
6.961ThrIle: 6.961 ± 2.426
3.867ThrLys: 3.867 ± 1.398
5.414ThrLeu: 5.414 ± 1.923
2.32ThrMet: 2.32 ± 0.923
0.0ThrAsn: 0.0 ± 0.0
4.64ThrPro: 4.64 ± 1.309
0.0ThrGln: 0.0 ± 0.0
2.32ThrArg: 2.32 ± 0.906
2.32ThrSer: 2.32 ± 1.131
3.094ThrThr: 3.094 ± 1.103
3.094ThrVal: 3.094 ± 1.915
2.32ThrTrp: 2.32 ± 0.906
1.547ThrTyr: 1.547 ± 0.71
0.0ThrXaa: 0.0 ± 0.0
Val
2.32ValAla: 2.32 ± 0.316
0.0ValCys: 0.0 ± 0.0
3.094ValAsp: 3.094 ± 1.391
3.094ValGlu: 3.094 ± 0.71
3.867ValPhe: 3.867 ± 0.954
2.32ValGly: 2.32 ± 0.953
6.187ValHis: 6.187 ± 1.22
1.547ValIle: 1.547 ± 0.925
6.187ValLys: 6.187 ± 1.806
5.414ValLeu: 5.414 ± 1.506
0.773ValMet: 0.773 ± 0.858
1.547ValAsn: 1.547 ± 0.674
4.64ValPro: 4.64 ± 0.942
2.32ValGln: 2.32 ± 1.008
4.64ValArg: 4.64 ± 1.652
3.867ValSer: 3.867 ± 1.035
3.094ValThr: 3.094 ± 1.348
1.547ValVal: 1.547 ± 0.71
0.773ValTrp: 0.773 ± 0.616
0.0ValTyr: 0.0 ± 0.0
0.0ValXaa: 0.0 ± 0.0
Trp
0.0TrpAla: 0.0 ± 0.0
0.0TrpCys: 0.0 ± 0.0
1.547TrpAsp: 1.547 ± 1.394
0.773TrpGlu: 0.773 ± 0.616
1.547TrpPhe: 1.547 ± 0.674
0.773TrpGly: 0.773 ± 0.616
0.0TrpHis: 0.0 ± 0.0
0.773TrpIle: 0.773 ± 0.697
2.32TrpLys: 2.32 ± 1.265
0.773TrpLeu: 0.773 ± 0.616
0.0TrpMet: 0.0 ± 0.0
0.773TrpAsn: 0.773 ± 0.616
1.547TrpPro: 1.547 ± 1.073
2.32TrpGln: 2.32 ± 0.316
1.547TrpArg: 1.547 ± 0.967
0.773TrpSer: 0.773 ± 0.544
0.0TrpThr: 0.0 ± 0.0
3.094TrpVal: 3.094 ± 1.09
1.547TrpTrp: 1.547 ± 1.232
0.773TrpTyr: 0.773 ± 0.616
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.094TyrAla: 3.094 ± 1.611
0.773TyrCys: 0.773 ± 0.616
2.32TyrAsp: 2.32 ± 1.848
2.32TyrGlu: 2.32 ± 0.316
1.547TyrPhe: 1.547 ± 0.551
3.867TyrGly: 3.867 ± 1.979
0.773TyrHis: 0.773 ± 0.697
0.773TyrIle: 0.773 ± 0.544
1.547TyrLys: 1.547 ± 1.089
3.094TyrLeu: 3.094 ± 0.538
0.773TyrMet: 0.773 ± 0.544
0.773TyrAsn: 0.773 ± 0.697
3.094TyrPro: 3.094 ± 1.09
0.773TyrGln: 0.773 ± 0.544
0.0TyrArg: 0.0 ± 0.0
1.547TyrSer: 1.547 ± 1.073
3.094TyrThr: 3.094 ± 0.71
0.773TyrVal: 0.773 ± 0.616
1.547TyrTrp: 1.547 ± 1.394
1.547TyrTyr: 1.547 ± 1.089
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4 proteins (1294 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski