Amino acid dipepetide frequency for Muribaculaceae bacterium Isolate-037 (Harlan)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.32AlaAla: 6.32 ± 0.108	0.851AlaCys: 0.851 ± 0.029	5.102AlaAsp: 5.102 ± 0.069	4.755AlaGlu: 4.755 ± 0.083	2.927AlaPhe: 2.927 ± 0.046	5.07AlaGly: 5.07 ± 0.068	1.173AlaHis: 1.173 ± 0.03	5.079AlaIle: 5.079 ± 0.073	4.133AlaLys: 4.133 ± 0.057	6.435AlaLeu: 6.435 ± 0.072	2.074AlaMet: 2.074 ± 0.045	3.297AlaAsn: 3.297 ± 0.061	2.483AlaPro: 2.483 ± 0.047	2.256AlaGln: 2.256 ± 0.05	3.358AlaArg: 3.358 ± 0.061	4.804AlaSer: 4.804 ± 0.067	4.227AlaThr: 4.227 ± 0.056	4.83AlaVal: 4.83 ± 0.069	0.868AlaTrp: 0.868 ± 0.026	2.797AlaTyr: 2.797 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.839CysAla: 0.839 ± 0.024	0.194CysCys: 0.194 ± 0.012	0.763CysAsp: 0.763 ± 0.024	0.637CysGlu: 0.637 ± 0.026	0.488CysPhe: 0.488 ± 0.019	1.163CysGly: 1.163 ± 0.031	0.306CysHis: 0.306 ± 0.014	0.785CysIle: 0.785 ± 0.024	0.583CysLys: 0.583 ± 0.02	0.958CysLeu: 0.958 ± 0.025	0.319CysMet: 0.319 ± 0.014	0.564CysAsn: 0.564 ± 0.021	0.488CysPro: 0.488 ± 0.022	0.29CysGln: 0.29 ± 0.015	0.815CysArg: 0.815 ± 0.024	0.83CysSer: 0.83 ± 0.025	0.53CysThr: 0.53 ± 0.019	0.798CysVal: 0.798 ± 0.025	0.161CysTrp: 0.161 ± 0.011	0.508CysTyr: 0.508 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	4.59AspAla: 4.59 ± 0.07	0.74AspCys: 0.74 ± 0.027	3.51AspAsp: 3.51 ± 0.065	4.04AspGlu: 4.04 ± 0.061	3.18AspPhe: 3.18 ± 0.052	5.114AspGly: 5.114 ± 0.075	0.906AspHis: 0.906 ± 0.029	4.486AspIle: 4.486 ± 0.059	4.098AspLys: 4.098 ± 0.062	4.781AspLeu: 4.781 ± 0.057	1.917AspMet: 1.917 ± 0.039	3.594AspAsn: 3.594 ± 0.066	2.313AspPro: 2.313 ± 0.04	1.087AspGln: 1.087 ± 0.031	3.115AspArg: 3.115 ± 0.047	4.233AspSer: 4.233 ± 0.063	2.924AspThr: 2.924 ± 0.05	3.934AspVal: 3.934 ± 0.061	0.947AspTrp: 0.947 ± 0.028	3.031AspTyr: 3.031 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	4.619GluAla: 4.619 ± 0.08	0.712GluCys: 0.712 ± 0.026	3.391GluAsp: 3.391 ± 0.052	4.647GluGlu: 4.647 ± 0.088	2.488GluPhe: 2.488 ± 0.05	3.979GluGly: 3.979 ± 0.067	1.011GluHis: 1.011 ± 0.027	5.061GluIle: 5.061 ± 0.065	4.549GluLys: 4.549 ± 0.067	5.478GluLeu: 5.478 ± 0.07	2.002GluMet: 2.002 ± 0.043	3.59GluAsn: 3.59 ± 0.059	1.927GluPro: 1.927 ± 0.041	1.955GluGln: 1.955 ± 0.042	3.478GluArg: 3.478 ± 0.058	3.882GluSer: 3.882 ± 0.057	3.244GluThr: 3.244 ± 0.051	3.867GluVal: 3.867 ± 0.059	0.969GluTrp: 0.969 ± 0.026	2.842GluTyr: 2.842 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	3.024PheAla: 3.024 ± 0.049	0.616PheCys: 0.616 ± 0.02	2.917PheAsp: 2.917 ± 0.052	2.313PheGlu: 2.313 ± 0.044	1.926PhePhe: 1.926 ± 0.04	3.312PheGly: 3.312 ± 0.062	0.85PheHis: 0.85 ± 0.027	3.01PheIle: 3.01 ± 0.058	2.323PheLys: 2.323 ± 0.045	3.483PheLeu: 3.483 ± 0.058	1.257PheMet: 1.257 ± 0.036	2.468PheAsn: 2.468 ± 0.047	1.684PhePro: 1.684 ± 0.037	1.037PheGln: 1.037 ± 0.03	2.262PheArg: 2.262 ± 0.038	3.569PheSer: 3.569 ± 0.054	2.574PheThr: 2.574 ± 0.04	2.587PheVal: 2.587 ± 0.049	0.534PheTrp: 0.534 ± 0.023	1.698PheTyr: 1.698 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	4.635GlyAla: 4.635 ± 0.068	0.992GlyCys: 0.992 ± 0.032	4.151GlyAsp: 4.151 ± 0.061	4.461GlyGlu: 4.461 ± 0.066	3.125GlyPhe: 3.125 ± 0.044	4.747GlyGly: 4.747 ± 0.086	1.247GlyHis: 1.247 ± 0.036	5.344GlyIle: 5.344 ± 0.068	5.222GlyLys: 5.222 ± 0.064	5.574GlyLeu: 5.574 ± 0.068	2.177GlyMet: 2.177 ± 0.042	4.074GlyAsn: 4.074 ± 0.068	1.312GlyPro: 1.312 ± 0.036	1.829GlyGln: 1.829 ± 0.039	3.263GlyArg: 3.263 ± 0.055	4.328GlySer: 4.328 ± 0.071	3.788GlyThr: 3.788 ± 0.069	4.915GlyVal: 4.915 ± 0.073	1.108GlyTrp: 1.108 ± 0.029	3.312GlyTyr: 3.312 ± 0.053	0.0GlyXaa: 0.0 ± 0.0
His	1.098HisAla: 1.098 ± 0.029	0.265HisCys: 0.265 ± 0.013	1.185HisAsp: 1.185 ± 0.032	1.004HisGlu: 1.004 ± 0.024	0.873HisPhe: 0.873 ± 0.025	1.247HisGly: 1.247 ± 0.032	0.452HisHis: 0.452 ± 0.023	1.347HisIle: 1.347 ± 0.034	0.971HisLys: 0.971 ± 0.03	1.579HisLeu: 1.579 ± 0.035	0.289HisMet: 0.289 ± 0.015	0.965HisAsn: 0.965 ± 0.025	0.978HisPro: 0.978 ± 0.028	0.524HisGln: 0.524 ± 0.02	0.956HisArg: 0.956 ± 0.027	1.166HisSer: 1.166 ± 0.032	1.001HisThr: 1.001 ± 0.026	0.996HisVal: 0.996 ± 0.026	0.249HisTrp: 0.249 ± 0.014	0.799HisTyr: 0.799 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.33IleAla: 5.33 ± 0.075	0.965IleCys: 0.965 ± 0.025	4.896IleAsp: 4.896 ± 0.064	4.499IleGlu: 4.499 ± 0.065	2.891IlePhe: 2.891 ± 0.052	4.751IleGly: 4.751 ± 0.069	1.147IleHis: 1.147 ± 0.033	4.607IleIle: 4.607 ± 0.077	3.976IleLys: 3.976 ± 0.066	5.699IleLeu: 5.699 ± 0.075	1.673IleMet: 1.673 ± 0.039	3.559IleAsn: 3.559 ± 0.051	3.257IlePro: 3.257 ± 0.051	1.738IleGln: 1.738 ± 0.041	3.428IleArg: 3.428 ± 0.057	5.413IleSer: 5.413 ± 0.066	3.872IleThr: 3.872 ± 0.055	4.468IleVal: 4.468 ± 0.06	0.762IleTrp: 0.762 ± 0.025	2.691IleTyr: 2.691 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	4.547LysAla: 4.547 ± 0.069	0.557LysCys: 0.557 ± 0.023	3.898LysAsp: 3.898 ± 0.066	4.741LysGlu: 4.741 ± 0.076	2.321LysPhe: 2.321 ± 0.041	4.281LysGly: 4.281 ± 0.064	0.96LysHis: 0.96 ± 0.027	4.308LysIle: 4.308 ± 0.062	4.086LysLys: 4.086 ± 0.071	4.727LysLeu: 4.727 ± 0.062	1.957LysMet: 1.957 ± 0.039	3.146LysAsn: 3.146 ± 0.055	2.139LysPro: 2.139 ± 0.039	1.768LysGln: 1.768 ± 0.037	3.279LysArg: 3.279 ± 0.058	3.716LysSer: 3.716 ± 0.051	3.33LysThr: 3.33 ± 0.049	3.97LysVal: 3.97 ± 0.057	0.841LysTrp: 0.841 ± 0.026	2.636LysTyr: 2.636 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	6.093LeuAla: 6.093 ± 0.07	1.209LeuCys: 1.209 ± 0.031	5.048LeuAsp: 5.048 ± 0.058	4.668LeuGlu: 4.668 ± 0.067	3.683LeuPhe: 3.683 ± 0.063	5.266LeuGly: 5.266 ± 0.071	1.683LeuHis: 1.683 ± 0.038	5.432LeuIle: 5.432 ± 0.067	5.451LeuLys: 5.451 ± 0.077	7.504LeuLeu: 7.504 ± 0.089	2.406LeuMet: 2.406 ± 0.044	4.488LeuAsn: 4.488 ± 0.059	4.221LeuPro: 4.221 ± 0.06	2.717LeuGln: 2.717 ± 0.049	4.762LeuArg: 4.762 ± 0.073	7.11LeuSer: 7.11 ± 0.084	5.25LeuThr: 5.25 ± 0.058	4.623LeuVal: 4.623 ± 0.068	1.054LeuTrp: 1.054 ± 0.028	3.324LeuTyr: 3.324 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.328MetAla: 2.328 ± 0.041	0.261MetCys: 0.261 ± 0.013	1.388MetAsp: 1.388 ± 0.032	1.796MetGlu: 1.796 ± 0.037	1.018MetPhe: 1.018 ± 0.029	1.664MetGly: 1.664 ± 0.034	0.43MetHis: 0.43 ± 0.017	1.76MetIle: 1.76 ± 0.034	2.194MetLys: 2.194 ± 0.044	2.625MetLeu: 2.625 ± 0.044	0.91MetMet: 0.91 ± 0.027	1.42MetAsn: 1.42 ± 0.028	1.355MetPro: 1.355 ± 0.028	0.974MetGln: 0.974 ± 0.025	1.61MetArg: 1.61 ± 0.037	1.84MetSer: 1.84 ± 0.036	1.776MetThr: 1.776 ± 0.031	1.71MetVal: 1.71 ± 0.037	0.29MetTrp: 0.29 ± 0.016	0.777MetTyr: 0.777 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	3.752AsnAla: 3.752 ± 0.056	0.541AsnCys: 0.541 ± 0.02	3.04AsnAsp: 3.04 ± 0.049	2.886AsnGlu: 2.886 ± 0.051	2.263AsnPhe: 2.263 ± 0.047	4.244AsnGly: 4.244 ± 0.061	1.038AsnHis: 1.038 ± 0.026	3.804AsnIle: 3.804 ± 0.066	2.733AsnLys: 2.733 ± 0.05	4.61AsnLeu: 4.61 ± 0.063	1.314AsnMet: 1.314 ± 0.031	2.705AsnAsn: 2.705 ± 0.056	2.899AsnPro: 2.899 ± 0.05	1.443AsnGln: 1.443 ± 0.037	2.717AsnArg: 2.717 ± 0.047	3.091AsnSer: 3.091 ± 0.05	2.572AsnThr: 2.572 ± 0.052	3.336AsnVal: 3.336 ± 0.057	0.695AsnTrp: 0.695 ± 0.024	2.313AsnTyr: 2.313 ± 0.049	0.0AsnXaa: 0.0 ± 0.0
Pro	2.887ProAla: 2.887 ± 0.051	0.369ProCys: 0.369 ± 0.015	3.125ProAsp: 3.125 ± 0.049	3.583ProGlu: 3.583 ± 0.048	1.808ProPhe: 1.808 ± 0.039	2.643ProGly: 2.643 ± 0.044	0.715ProHis: 0.715 ± 0.024	2.407ProIle: 2.407 ± 0.041	2.049ProLys: 2.049 ± 0.044	3.222ProLeu: 3.222 ± 0.057	1.027ProMet: 1.027 ± 0.028	1.676ProAsn: 1.676 ± 0.032	0.908ProPro: 0.908 ± 0.031	1.371ProGln: 1.371 ± 0.03	1.51ProArg: 1.51 ± 0.034	2.693ProSer: 2.693 ± 0.043	2.282ProThr: 2.282 ± 0.047	2.93ProVal: 2.93 ± 0.049	0.498ProTrp: 0.498 ± 0.018	1.636ProTyr: 1.636 ± 0.039	0.0ProXaa: 0.0 ± 0.0
Gln	1.949GlnAla: 1.949 ± 0.042	0.28GlnCys: 0.28 ± 0.016	1.458GlnAsp: 1.458 ± 0.035	1.798GlnGlu: 1.798 ± 0.035	1.231GlnPhe: 1.231 ± 0.029	1.75GlnGly: 1.75 ± 0.034	0.489GlnHis: 0.489 ± 0.019	2.038GlnIle: 2.038 ± 0.039	1.825GlnLys: 1.825 ± 0.04	2.803GlnLeu: 2.803 ± 0.05	0.887GlnMet: 0.887 ± 0.026	1.517GlnAsn: 1.517 ± 0.04	1.111GlnPro: 1.111 ± 0.03	1.179GlnGln: 1.179 ± 0.038	1.662GlnArg: 1.662 ± 0.036	1.809GlnSer: 1.809 ± 0.038	1.756GlnThr: 1.756 ± 0.037	1.55GlnVal: 1.55 ± 0.034	0.449GlnTrp: 0.449 ± 0.019	1.287GlnTyr: 1.287 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	2.918ArgAla: 2.918 ± 0.05	0.548ArgCys: 0.548 ± 0.021	2.779ArgAsp: 2.779 ± 0.047	3.339ArgGlu: 3.339 ± 0.061	2.485ArgPhe: 2.485 ± 0.048	2.815ArgGly: 2.815 ± 0.043	1.225ArgHis: 1.225 ± 0.032	3.973ArgIle: 3.973 ± 0.054	3.56ArgLys: 3.56 ± 0.053	5.14ArgLeu: 5.14 ± 0.075	1.598ArgMet: 1.598 ± 0.036	2.865ArgAsn: 2.865 ± 0.048	1.782ArgPro: 1.782 ± 0.034	1.953ArgGln: 1.953 ± 0.045	3.096ArgArg: 3.096 ± 0.061	2.634ArgSer: 2.634 ± 0.048	2.386ArgThr: 2.386 ± 0.042	2.862ArgVal: 2.862 ± 0.046	0.703ArgTrp: 0.703 ± 0.023	2.397ArgTyr: 2.397 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	4.749SerAla: 4.749 ± 0.065	0.829SerCys: 0.829 ± 0.022	4.499SerAsp: 4.499 ± 0.062	3.998SerGlu: 3.998 ± 0.06	3.244SerPhe: 3.244 ± 0.053	5.196SerGly: 5.196 ± 0.073	1.299SerHis: 1.299 ± 0.03	4.537SerIle: 4.537 ± 0.057	3.602SerLys: 3.602 ± 0.06	6.423SerLeu: 6.423 ± 0.081	1.76SerMet: 1.76 ± 0.04	3.079SerAsn: 3.079 ± 0.059	2.815SerPro: 2.815 ± 0.056	1.963SerGln: 1.963 ± 0.035	3.235SerArg: 3.235 ± 0.058	4.369SerSer: 4.369 ± 0.073	3.474SerThr: 3.474 ± 0.058	4.77SerVal: 4.77 ± 0.074	0.886SerTrp: 0.886 ± 0.026	2.762SerTyr: 2.762 ± 0.05	0.0SerXaa: 0.0 ± 0.0
Thr	4.137ThrAla: 4.137 ± 0.062	0.493ThrCys: 0.493 ± 0.018	3.838ThrAsp: 3.838 ± 0.059	3.178ThrGlu: 3.178 ± 0.056	2.471ThrPhe: 2.471 ± 0.045	4.213ThrGly: 4.213 ± 0.061	0.969ThrHis: 0.969 ± 0.029	3.747ThrIle: 3.747 ± 0.056	2.658ThrLys: 2.658 ± 0.05	5.203ThrLeu: 5.203 ± 0.062	1.221ThrMet: 1.221 ± 0.03	2.323ThrAsn: 2.323 ± 0.046	3.008ThrPro: 3.008 ± 0.053	1.518ThrGln: 1.518 ± 0.033	2.347ThrArg: 2.347 ± 0.041	3.496ThrSer: 3.496 ± 0.056	3.201ThrThr: 3.201 ± 0.063	4.056ThrVal: 4.056 ± 0.058	0.628ThrTrp: 0.628 ± 0.023	2.282ThrTyr: 2.282 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	5.294ValAla: 5.294 ± 0.077	0.808ValCys: 0.808 ± 0.025	3.939ValAsp: 3.939 ± 0.064	4.245ValGlu: 4.245 ± 0.064	2.55ValPhe: 2.55 ± 0.046	4.125ValGly: 4.125 ± 0.067	0.873ValHis: 0.873 ± 0.023	4.363ValIle: 4.363 ± 0.06	4.163ValLys: 4.163 ± 0.056	4.849ValLeu: 4.849 ± 0.061	1.894ValMet: 1.894 ± 0.042	3.419ValAsn: 3.419 ± 0.055	2.528ValPro: 2.528 ± 0.049	1.493ValGln: 1.493 ± 0.03	3.112ValArg: 3.112 ± 0.045	4.703ValSer: 4.703 ± 0.064	3.822ValThr: 3.822 ± 0.059	4.633ValVal: 4.633 ± 0.071	0.727ValTrp: 0.727 ± 0.023	2.478ValTyr: 2.478 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	0.784TrpAla: 0.784 ± 0.024	0.192TrpCys: 0.192 ± 0.013	0.774TrpAsp: 0.774 ± 0.027	0.824TrpGlu: 0.824 ± 0.025	0.552TrpPhe: 0.552 ± 0.022	0.983TrpGly: 0.983 ± 0.03	0.292TrpHis: 0.292 ± 0.015	0.896TrpIle: 0.896 ± 0.028	0.816TrpLys: 0.816 ± 0.028	1.265TrpLeu: 1.265 ± 0.038	0.404TrpMet: 0.404 ± 0.018	0.853TrpAsn: 0.853 ± 0.029	0.246TrpPro: 0.246 ± 0.014	0.521TrpGln: 0.521 ± 0.022	0.727TrpArg: 0.727 ± 0.023	0.821TrpSer: 0.821 ± 0.027	0.704TrpThr: 0.704 ± 0.026	0.75TrpVal: 0.75 ± 0.024	0.264TrpTrp: 0.264 ± 0.016	0.518TrpTyr: 0.518 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.944TyrAla: 2.944 ± 0.048	0.595TyrCys: 0.595 ± 0.019	2.836TyrAsp: 2.836 ± 0.058	2.28TyrGlu: 2.28 ± 0.04	1.922TyrPhe: 1.922 ± 0.035	3.04TyrGly: 3.04 ± 0.054	0.884TyrHis: 0.884 ± 0.027	2.639TyrIle: 2.639 ± 0.058	2.215TyrLys: 2.215 ± 0.044	3.635TyrLeu: 3.635 ± 0.05	1.039TyrMet: 1.039 ± 0.03	2.393TyrAsn: 2.393 ± 0.052	1.886TyrPro: 1.886 ± 0.04	1.217TyrGln: 1.217 ± 0.033	2.325TyrArg: 2.325 ± 0.038	2.984TyrSer: 2.984 ± 0.054	2.273TyrThr: 2.273 ± 0.053	2.43TyrVal: 2.43 ± 0.036	0.55TyrTrp: 0.55 ± 0.021	1.985TyrTyr: 1.985 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3984 proteins (1419065 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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