Amino acid dipepetide frequency for Alkalispirochaeta americana

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.534AlaAla: 9.534 ± 0.154	0.798AlaCys: 0.798 ± 0.033	4.414AlaAsp: 4.414 ± 0.063	6.011AlaGlu: 6.011 ± 0.083	3.65AlaPhe: 3.65 ± 0.071	8.598AlaGly: 8.598 ± 0.119	1.612AlaHis: 1.612 ± 0.038	4.679AlaIle: 4.679 ± 0.068	1.952AlaLys: 1.952 ± 0.049	11.271AlaLeu: 11.271 ± 0.145	2.135AlaMet: 2.135 ± 0.055	1.84AlaAsn: 1.84 ± 0.048	4.055AlaPro: 4.055 ± 0.081	3.041AlaGln: 3.041 ± 0.057	7.662AlaArg: 7.662 ± 0.102	5.167AlaSer: 5.167 ± 0.079	4.323AlaThr: 4.323 ± 0.076	7.36AlaVal: 7.36 ± 0.099	0.984AlaTrp: 0.984 ± 0.031	1.98AlaTyr: 1.98 ± 0.045	0.0AlaXaa: 0.0 ± 0.0
Cys	0.786CysAla: 0.786 ± 0.032	0.132CysCys: 0.132 ± 0.011	0.485CysAsp: 0.485 ± 0.023	0.421CysGlu: 0.421 ± 0.021	0.348CysPhe: 0.348 ± 0.018	0.838CysGly: 0.838 ± 0.033	0.196CysHis: 0.196 ± 0.014	0.398CysIle: 0.398 ± 0.022	0.179CysLys: 0.179 ± 0.012	0.836CysLeu: 0.836 ± 0.029	0.132CysMet: 0.132 ± 0.01	0.222CysAsn: 0.222 ± 0.012	0.542CysPro: 0.542 ± 0.024	0.279CysGln: 0.279 ± 0.016	0.741CysArg: 0.741 ± 0.03	0.643CysSer: 0.643 ± 0.027	0.428CysThr: 0.428 ± 0.024	0.474CysVal: 0.474 ± 0.023	0.079CysTrp: 0.079 ± 0.009	0.239CysTyr: 0.239 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	4.139AspAla: 4.139 ± 0.079	0.427AspCys: 0.427 ± 0.023	2.932AspAsp: 2.932 ± 0.074	3.669AspGlu: 3.669 ± 0.08	2.229AspPhe: 2.229 ± 0.056	3.648AspGly: 3.648 ± 0.075	1.417AspHis: 1.417 ± 0.038	3.13AspIle: 3.13 ± 0.068	1.192AspLys: 1.192 ± 0.042	6.696AspLeu: 6.696 ± 0.087	1.099AspMet: 1.099 ± 0.035	1.31AspAsn: 1.31 ± 0.042	3.607AspPro: 3.607 ± 0.066	2.685AspGln: 2.685 ± 0.045	4.323AspArg: 4.323 ± 0.082	3.168AspSer: 3.168 ± 0.067	2.731AspThr: 2.731 ± 0.052	3.16AspVal: 3.16 ± 0.061	0.664AspTrp: 0.664 ± 0.025	1.799AspTyr: 1.799 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	7.067GluAla: 7.067 ± 0.098	0.357GluCys: 0.357 ± 0.02	3.801GluAsp: 3.801 ± 0.077	6.623GluGlu: 6.623 ± 0.117	2.133GluPhe: 2.133 ± 0.047	5.445GluGly: 5.445 ± 0.076	1.337GluHis: 1.337 ± 0.034	5.037GluIle: 5.037 ± 0.074	3.071GluLys: 3.071 ± 0.06	7.035GluLeu: 7.035 ± 0.113	1.778GluMet: 1.778 ± 0.042	2.179GluAsn: 2.179 ± 0.049	2.809GluPro: 2.809 ± 0.064	2.907GluGln: 2.907 ± 0.064	5.359GluArg: 5.359 ± 0.082	3.886GluSer: 3.886 ± 0.057	4.165GluThr: 4.165 ± 0.07	4.582GluVal: 4.582 ± 0.078	0.682GluTrp: 0.682 ± 0.026	1.819GluTyr: 1.819 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	3.174PheAla: 3.174 ± 0.054	0.469PheCys: 0.469 ± 0.022	2.205PheAsp: 2.205 ± 0.05	2.192PheGlu: 2.192 ± 0.053	2.408PhePhe: 2.408 ± 0.06	3.129PheGly: 3.129 ± 0.068	0.974PheHis: 0.974 ± 0.036	1.873PheIle: 1.873 ± 0.047	0.743PheLys: 0.743 ± 0.029	5.051PheLeu: 5.051 ± 0.09	0.683PheMet: 0.683 ± 0.029	0.997PheAsn: 0.997 ± 0.035	2.202PhePro: 2.202 ± 0.05	1.511PheGln: 1.511 ± 0.036	3.041PheArg: 3.041 ± 0.057	3.68PheSer: 3.68 ± 0.066	2.034PheThr: 2.034 ± 0.054	2.477PheVal: 2.477 ± 0.05	0.515PheTrp: 0.515 ± 0.025	1.087PheTyr: 1.087 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	7.324GlyAla: 7.324 ± 0.095	0.784GlyCys: 0.784 ± 0.03	4.426GlyAsp: 4.426 ± 0.079	5.763GlyGlu: 5.763 ± 0.077	3.399GlyPhe: 3.399 ± 0.061	6.566GlyGly: 6.566 ± 0.112	1.551GlyHis: 1.551 ± 0.045	5.257GlyIle: 5.257 ± 0.087	2.975GlyLys: 2.975 ± 0.072	7.809GlyLeu: 7.809 ± 0.093	2.139GlyMet: 2.139 ± 0.045	2.341GlyAsn: 2.341 ± 0.068	2.848GlyPro: 2.848 ± 0.056	2.606GlyGln: 2.606 ± 0.052	5.567GlyArg: 5.567 ± 0.087	5.087GlySer: 5.087 ± 0.083	4.804GlyThr: 4.804 ± 0.091	6.179GlyVal: 6.179 ± 0.097	0.997GlyTrp: 0.997 ± 0.035	2.429GlyTyr: 2.429 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	1.487HisAla: 1.487 ± 0.04	0.21HisCys: 0.21 ± 0.016	1.038HisAsp: 1.038 ± 0.033	1.081HisGlu: 1.081 ± 0.031	0.872HisPhe: 0.872 ± 0.031	1.491HisGly: 1.491 ± 0.037	0.694HisHis: 0.694 ± 0.032	1.073HisIle: 1.073 ± 0.033	0.467HisLys: 0.467 ± 0.022	2.565HisLeu: 2.565 ± 0.06	0.349HisMet: 0.349 ± 0.018	0.583HisAsn: 0.583 ± 0.028	1.475HisPro: 1.475 ± 0.042	1.1HisGln: 1.1 ± 0.035	1.975HisArg: 1.975 ± 0.044	1.174HisSer: 1.174 ± 0.031	1.0HisThr: 1.0 ± 0.032	1.134HisVal: 1.134 ± 0.032	0.249HisTrp: 0.249 ± 0.016	0.69HisTyr: 0.69 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.386IleAla: 5.386 ± 0.08	0.516IleCys: 0.516 ± 0.024	3.235IleAsp: 3.235 ± 0.06	3.376IleGlu: 3.376 ± 0.062	2.309IlePhe: 2.309 ± 0.05	3.952IleGly: 3.952 ± 0.078	1.266IleHis: 1.266 ± 0.037	3.083IleIle: 3.083 ± 0.062	1.542IleLys: 1.542 ± 0.043	6.177IleLeu: 6.177 ± 0.087	1.148IleMet: 1.148 ± 0.038	1.642IleAsn: 1.642 ± 0.042	3.414IlePro: 3.414 ± 0.065	1.895IleGln: 1.895 ± 0.041	3.81IleArg: 3.81 ± 0.063	3.991IleSer: 3.991 ± 0.059	3.636IleThr: 3.636 ± 0.073	3.699IleVal: 3.699 ± 0.069	0.5IleTrp: 0.5 ± 0.023	1.346IleTyr: 1.346 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	2.61LysAla: 2.61 ± 0.057	0.167LysCys: 0.167 ± 0.013	1.759LysAsp: 1.759 ± 0.047	2.454LysGlu: 2.454 ± 0.054	0.666LysPhe: 0.666 ± 0.026	2.186LysGly: 2.186 ± 0.053	0.622LysHis: 0.622 ± 0.027	2.058LysIle: 2.058 ± 0.051	1.753LysLys: 1.753 ± 0.056	2.423LysLeu: 2.423 ± 0.056	0.76LysMet: 0.76 ± 0.03	1.271LysAsn: 1.271 ± 0.037	1.411LysPro: 1.411 ± 0.036	0.986LysGln: 0.986 ± 0.035	2.154LysArg: 2.154 ± 0.051	1.671LysSer: 1.671 ± 0.046	2.092LysThr: 2.092 ± 0.05	1.883LysVal: 1.883 ± 0.043	0.193LysTrp: 0.193 ± 0.013	0.687LysTyr: 0.687 ± 0.029	0.0LysXaa: 0.0 ± 0.0
Leu	10.983LeuAla: 10.983 ± 0.14	0.963LeuCys: 0.963 ± 0.033	5.827LeuAsp: 5.827 ± 0.099	8.513LeuGlu: 8.513 ± 0.12	4.565LeuPhe: 4.565 ± 0.088	8.327LeuGly: 8.327 ± 0.11	2.147LeuHis: 2.147 ± 0.048	4.974LeuIle: 4.974 ± 0.083	3.304LeuLys: 3.304 ± 0.065	12.115LeuLeu: 12.115 ± 0.165	1.939LeuMet: 1.939 ± 0.041	2.643LeuAsn: 2.643 ± 0.052	5.73LeuPro: 5.73 ± 0.086	4.53LeuGln: 4.53 ± 0.071	8.244LeuArg: 8.244 ± 0.108	7.225LeuSer: 7.225 ± 0.103	4.922LeuThr: 4.922 ± 0.086	8.274LeuVal: 8.274 ± 0.106	1.199LeuTrp: 1.199 ± 0.039	2.682LeuTyr: 2.682 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	2.127MetAla: 2.127 ± 0.051	0.101MetCys: 0.101 ± 0.01	1.136MetAsp: 1.136 ± 0.036	1.5MetGlu: 1.5 ± 0.036	0.574MetPhe: 0.574 ± 0.023	1.52MetGly: 1.52 ± 0.047	0.36MetHis: 0.36 ± 0.018	1.591MetIle: 1.591 ± 0.04	1.194MetLys: 1.194 ± 0.036	1.791MetLeu: 1.791 ± 0.043	0.525MetMet: 0.525 ± 0.024	0.967MetAsn: 0.967 ± 0.03	0.98MetPro: 0.98 ± 0.034	0.702MetGln: 0.702 ± 0.029	1.316MetArg: 1.316 ± 0.034	1.236MetSer: 1.236 ± 0.036	1.425MetThr: 1.425 ± 0.041	1.809MetVal: 1.809 ± 0.039	0.137MetTrp: 0.137 ± 0.012	0.433MetTyr: 0.433 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.213AsnAla: 2.213 ± 0.057	0.227AsnCys: 0.227 ± 0.014	1.335AsnAsp: 1.335 ± 0.05	1.43AsnGlu: 1.43 ± 0.037	0.998AsnPhe: 0.998 ± 0.036	1.734AsnGly: 1.734 ± 0.053	0.74AsnHis: 0.74 ± 0.027	1.666AsnIle: 1.666 ± 0.042	0.798AsnLys: 0.798 ± 0.032	3.242AsnLeu: 3.242 ± 0.067	0.616AsnMet: 0.616 ± 0.025	0.899AsnAsn: 0.899 ± 0.037	1.967AsnPro: 1.967 ± 0.044	1.131AsnGln: 1.131 ± 0.033	2.321AsnArg: 2.321 ± 0.045	1.542AsnSer: 1.542 ± 0.043	1.494AsnThr: 1.494 ± 0.047	1.646AsnVal: 1.646 ± 0.047	0.33AsnTrp: 0.33 ± 0.021	0.835AsnTyr: 0.835 ± 0.033	0.0AsnXaa: 0.0 ± 0.0
Pro	4.379ProAla: 4.379 ± 0.077	0.348ProCys: 0.348 ± 0.018	3.303ProAsp: 3.303 ± 0.064	4.73ProGlu: 4.73 ± 0.086	1.968ProPhe: 1.968 ± 0.047	5.594ProGly: 5.594 ± 0.086	1.017ProHis: 1.017 ± 0.035	1.68ProIle: 1.68 ± 0.042	0.98ProLys: 0.98 ± 0.034	5.894ProLeu: 5.894 ± 0.094	0.835ProMet: 0.835 ± 0.025	0.91ProAsn: 0.91 ± 0.027	2.875ProPro: 2.875 ± 0.074	1.838ProGln: 1.838 ± 0.047	3.792ProArg: 3.792 ± 0.071	3.008ProSer: 3.008 ± 0.053	1.597ProThr: 1.597 ± 0.043	3.933ProVal: 3.933 ± 0.062	0.735ProTrp: 0.735 ± 0.028	1.162ProTyr: 1.162 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	3.868GlnAla: 3.868 ± 0.077	0.195GlnCys: 0.195 ± 0.014	2.25GlnAsp: 2.25 ± 0.049	4.049GlnGlu: 4.049 ± 0.072	1.138GlnPhe: 1.138 ± 0.038	3.749GlnGly: 3.749 ± 0.065	0.659GlnHis: 0.659 ± 0.027	2.148GlnIle: 2.148 ± 0.045	1.426GlnLys: 1.426 ± 0.042	2.864GlnLeu: 2.864 ± 0.053	0.764GlnMet: 0.764 ± 0.027	1.156GlnAsn: 1.156 ± 0.037	1.362GlnPro: 1.362 ± 0.035	1.461GlnGln: 1.461 ± 0.046	2.747GlnArg: 2.747 ± 0.057	1.8GlnSer: 1.8 ± 0.045	1.87GlnThr: 1.87 ± 0.044	2.76GlnVal: 2.76 ± 0.05	0.426GlnTrp: 0.426 ± 0.021	0.875GlnTyr: 0.875 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	6.029ArgAla: 6.029 ± 0.089	0.64ArgCys: 0.64 ± 0.026	4.58ArgAsp: 4.58 ± 0.066	6.935ArgGlu: 6.935 ± 0.087	3.382ArgPhe: 3.382 ± 0.064	5.68ArgGly: 5.68 ± 0.088	1.606ArgHis: 1.606 ± 0.039	4.596ArgIle: 4.596 ± 0.066	2.705ArgLys: 2.705 ± 0.06	7.526ArgLeu: 7.526 ± 0.1	1.65ArgMet: 1.65 ± 0.036	2.328ArgAsn: 2.328 ± 0.055	3.04ArgPro: 3.04 ± 0.059	3.121ArgGln: 3.121 ± 0.064	6.002ArgArg: 6.002 ± 0.101	4.764ArgSer: 4.764 ± 0.075	3.638ArgThr: 3.638 ± 0.061	4.991ArgVal: 4.991 ± 0.078	1.059ArgTrp: 1.059 ± 0.037	2.453ArgTyr: 2.453 ± 0.057	0.0ArgXaa: 0.0 ± 0.0
Ser	5.265SerAla: 5.265 ± 0.079	0.678SerCys: 0.678 ± 0.026	3.004SerAsp: 3.004 ± 0.061	3.377SerGlu: 3.377 ± 0.062	3.08SerPhe: 3.08 ± 0.055	5.862SerGly: 5.862 ± 0.086	1.194SerHis: 1.194 ± 0.034	3.157SerIle: 3.157 ± 0.062	1.278SerLys: 1.278 ± 0.031	7.518SerLeu: 7.518 ± 0.091	1.365SerMet: 1.365 ± 0.036	1.372SerAsn: 1.372 ± 0.044	3.71SerPro: 3.71 ± 0.071	2.018SerGln: 2.018 ± 0.048	5.542SerArg: 5.542 ± 0.088	4.582SerSer: 4.582 ± 0.08	2.796SerThr: 2.796 ± 0.059	4.499SerVal: 4.499 ± 0.077	0.976SerTrp: 0.976 ± 0.034	1.597SerTyr: 1.597 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.059ThrAla: 5.059 ± 0.072	0.357ThrCys: 0.357 ± 0.017	2.579ThrAsp: 2.579 ± 0.058	2.941ThrGlu: 2.941 ± 0.061	2.016ThrPhe: 2.016 ± 0.05	5.036ThrGly: 5.036 ± 0.089	0.926ThrHis: 0.926 ± 0.032	3.309ThrIle: 3.309 ± 0.082	1.201ThrLys: 1.201 ± 0.039	5.973ThrLeu: 5.973 ± 0.089	1.153ThrMet: 1.153 ± 0.038	1.299ThrAsn: 1.299 ± 0.044	3.093ThrPro: 3.093 ± 0.066	1.291ThrGln: 1.291 ± 0.042	3.587ThrArg: 3.587 ± 0.06	2.983ThrSer: 2.983 ± 0.062	2.801ThrThr: 2.801 ± 0.066	4.444ThrVal: 4.444 ± 0.083	0.587ThrTrp: 0.587 ± 0.023	1.184ThrTyr: 1.184 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	6.74ValAla: 6.74 ± 0.105	0.644ValCys: 0.644 ± 0.028	3.622ValAsp: 3.622 ± 0.068	4.702ValGlu: 4.702 ± 0.067	3.09ValPhe: 3.09 ± 0.062	4.563ValGly: 4.563 ± 0.082	1.424ValHis: 1.424 ± 0.039	4.26ValIle: 4.26 ± 0.07	2.003ValLys: 2.003 ± 0.052	8.22ValLeu: 8.22 ± 0.109	1.599ValMet: 1.599 ± 0.044	1.929ValAsn: 1.929 ± 0.047	3.644ValPro: 3.644 ± 0.064	2.63ValGln: 2.63 ± 0.053	4.933ValArg: 4.933 ± 0.081	4.939ValSer: 4.939 ± 0.09	4.034ValThr: 4.034 ± 0.08	5.945ValVal: 5.945 ± 0.111	0.7ValTrp: 0.7 ± 0.026	1.846ValTyr: 1.846 ± 0.043	0.0ValXaa: 0.0 ± 0.0
Trp	0.841TrpAla: 0.841 ± 0.027	0.112TrpCys: 0.112 ± 0.01	0.663TrpAsp: 0.663 ± 0.029	0.802TrpGlu: 0.802 ± 0.028	0.537TrpPhe: 0.537 ± 0.025	0.877TrpGly: 0.877 ± 0.032	0.285TrpHis: 0.285 ± 0.014	0.719TrpIle: 0.719 ± 0.025	0.438TrpLys: 0.438 ± 0.021	1.039TrpLeu: 1.039 ± 0.036	0.307TrpMet: 0.307 ± 0.018	0.475TrpAsn: 0.475 ± 0.022	0.473TrpPro: 0.473 ± 0.023	0.525TrpGln: 0.525 ± 0.025	0.793TrpArg: 0.793 ± 0.028	0.745TrpSer: 0.745 ± 0.026	0.57TrpThr: 0.57 ± 0.03	0.744TrpVal: 0.744 ± 0.027	0.2TrpTrp: 0.2 ± 0.014	0.41TrpTyr: 0.41 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.054TyrAla: 2.054 ± 0.049	0.273TyrCys: 0.273 ± 0.017	1.432TyrAsp: 1.432 ± 0.039	1.502TyrGlu: 1.502 ± 0.042	1.08TyrPhe: 1.08 ± 0.033	2.055TyrGly: 2.055 ± 0.052	0.684TyrHis: 0.684 ± 0.026	1.236TyrIle: 1.236 ± 0.038	0.61TyrLys: 0.61 ± 0.029	3.063TyrLeu: 3.063 ± 0.059	0.445TyrMet: 0.445 ± 0.025	0.801TyrAsn: 0.801 ± 0.031	1.34TyrPro: 1.34 ± 0.031	1.308TyrGln: 1.308 ± 0.037	2.814TyrArg: 2.814 ± 0.052	1.578TyrSer: 1.578 ± 0.044	1.46TyrThr: 1.46 ± 0.035	1.474TyrVal: 1.474 ± 0.038	0.343TyrTrp: 0.343 ± 0.02	0.89TyrTyr: 0.89 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2891 proteins (1021938 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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