Amino acid dipepetide frequency for Synechococcus phage S-RIP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.363AlaAla: 10.363 ± 1.076
0.715AlaCys: 0.715 ± 0.236
6.253AlaAsp: 6.253 ± 1.069
6.7AlaGlu: 6.7 ± 0.914
3.305AlaPhe: 3.305 ± 0.507
6.343AlaGly: 6.343 ± 0.852
1.251AlaHis: 1.251 ± 0.321
5.539AlaIle: 5.539 ± 0.731
4.377AlaLys: 4.377 ± 0.655
8.129AlaLeu: 8.129 ± 1.02
2.501AlaMet: 2.501 ± 0.525
4.377AlaAsn: 4.377 ± 0.541
4.02AlaPro: 4.02 ± 0.616
4.109AlaGln: 4.109 ± 0.628
5.539AlaArg: 5.539 ± 1.111
5.003AlaSer: 5.003 ± 0.525
5.539AlaThr: 5.539 ± 0.823
5.36AlaVal: 5.36 ± 0.938
0.715AlaTrp: 0.715 ± 0.217
3.841AlaTyr: 3.841 ± 0.568
0.0AlaXaa: 0.0 ± 0.0
Cys
0.536CysAla: 0.536 ± 0.241
0.357CysCys: 0.357 ± 0.179
0.715CysAsp: 0.715 ± 0.331
0.893CysGlu: 0.893 ± 0.299
0.268CysPhe: 0.268 ± 0.164
0.625CysGly: 0.625 ± 0.22
0.536CysHis: 0.536 ± 0.237
0.715CysIle: 0.715 ± 0.262
1.161CysLys: 1.161 ± 0.389
0.893CysLeu: 0.893 ± 0.359
0.179CysMet: 0.179 ± 0.124
0.447CysAsn: 0.447 ± 0.215
0.357CysPro: 0.357 ± 0.184
0.268CysGln: 0.268 ± 0.183
0.536CysArg: 0.536 ± 0.236
0.536CysSer: 0.536 ± 0.312
0.625CysThr: 0.625 ± 0.28
0.536CysVal: 0.536 ± 0.24
0.089CysTrp: 0.089 ± 0.095
0.536CysTyr: 0.536 ± 0.273
0.0CysXaa: 0.0 ± 0.0
Asp
6.164AspAla: 6.164 ± 1.061
1.161AspCys: 1.161 ± 0.312
3.395AspAsp: 3.395 ± 0.585
4.377AspGlu: 4.377 ± 0.669
2.859AspPhe: 2.859 ± 0.468
4.467AspGly: 4.467 ± 0.755
0.893AspHis: 0.893 ± 0.344
3.305AspIle: 3.305 ± 0.615
3.484AspLys: 3.484 ± 0.663
5.271AspLeu: 5.271 ± 0.883
0.983AspMet: 0.983 ± 0.304
1.697AspAsn: 1.697 ± 0.285
3.395AspPro: 3.395 ± 0.587
2.769AspGln: 2.769 ± 0.586
2.948AspArg: 2.948 ± 0.541
3.305AspSer: 3.305 ± 0.497
3.216AspThr: 3.216 ± 0.615
3.663AspVal: 3.663 ± 0.597
1.161AspTrp: 1.161 ± 0.337
2.859AspTyr: 2.859 ± 0.506
0.0AspXaa: 0.0 ± 0.0
Glu
7.147GluAla: 7.147 ± 0.942
0.625GluCys: 0.625 ± 0.251
3.752GluAsp: 3.752 ± 0.77
4.199GluGlu: 4.199 ± 0.729
2.412GluPhe: 2.412 ± 0.391
3.663GluGly: 3.663 ± 0.512
1.251GluHis: 1.251 ± 0.357
2.68GluIle: 2.68 ± 0.482
3.305GluLys: 3.305 ± 0.544
6.611GluLeu: 6.611 ± 0.685
2.412GluMet: 2.412 ± 0.414
2.323GluAsn: 2.323 ± 0.459
2.144GluPro: 2.144 ± 0.45
4.02GluGln: 4.02 ± 0.626
4.467GluArg: 4.467 ± 0.753
3.127GluSer: 3.127 ± 0.423
3.752GluThr: 3.752 ± 0.489
3.841GluVal: 3.841 ± 0.623
1.161GluTrp: 1.161 ± 0.306
2.323GluTyr: 2.323 ± 0.554
0.0GluXaa: 0.0 ± 0.0
Phe
2.233PheAla: 2.233 ± 0.444
0.447PheCys: 0.447 ± 0.189
2.323PheAsp: 2.323 ± 0.522
1.608PheGlu: 1.608 ± 0.413
1.519PhePhe: 1.519 ± 0.467
3.127PheGly: 3.127 ± 0.669
0.625PheHis: 0.625 ± 0.227
2.144PheIle: 2.144 ± 0.426
1.876PheLys: 1.876 ± 0.438
2.859PheLeu: 2.859 ± 0.513
1.608PheMet: 1.608 ± 0.36
2.412PheAsn: 2.412 ± 0.601
0.804PhePro: 0.804 ± 0.234
1.697PheGln: 1.697 ± 0.349
2.233PheArg: 2.233 ± 0.391
2.412PheSer: 2.412 ± 0.412
2.323PheThr: 2.323 ± 0.415
2.591PheVal: 2.591 ± 0.282
0.268PheTrp: 0.268 ± 0.169
0.983PheTyr: 0.983 ± 0.279
0.0PheXaa: 0.0 ± 0.0
Gly
6.253GlyAla: 6.253 ± 0.626
0.625GlyCys: 0.625 ± 0.227
4.824GlyAsp: 4.824 ± 0.594
4.02GlyGlu: 4.02 ± 0.677
3.127GlyPhe: 3.127 ± 0.579
4.735GlyGly: 4.735 ± 0.883
0.625GlyHis: 0.625 ± 0.197
3.752GlyIle: 3.752 ± 0.632
4.467GlyLys: 4.467 ± 0.541
5.092GlyLeu: 5.092 ± 0.5
1.965GlyMet: 1.965 ± 0.393
3.305GlyAsn: 3.305 ± 0.618
1.965GlyPro: 1.965 ± 0.365
2.501GlyGln: 2.501 ± 0.583
3.395GlyArg: 3.395 ± 0.498
4.824GlySer: 4.824 ± 0.877
5.628GlyThr: 5.628 ± 0.923
5.36GlyVal: 5.36 ± 0.712
1.519GlyTrp: 1.519 ± 0.31
3.037GlyTyr: 3.037 ± 0.446
0.0GlyXaa: 0.0 ± 0.0
His
1.161HisAla: 1.161 ± 0.312
0.357HisCys: 0.357 ± 0.187
1.34HisAsp: 1.34 ± 0.36
1.072HisGlu: 1.072 ± 0.306
0.715HisPhe: 0.715 ± 0.277
1.072HisGly: 1.072 ± 0.388
0.625HisHis: 0.625 ± 0.231
0.715HisIle: 0.715 ± 0.221
0.804HisLys: 0.804 ± 0.231
1.251HisLeu: 1.251 ± 0.495
0.447HisMet: 0.447 ± 0.226
1.072HisAsn: 1.072 ± 0.289
0.893HisPro: 0.893 ± 0.322
0.715HisGln: 0.715 ± 0.226
0.536HisArg: 0.536 ± 0.215
0.715HisSer: 0.715 ± 0.294
0.983HisThr: 0.983 ± 0.358
0.893HisVal: 0.893 ± 0.255
0.089HisTrp: 0.089 ± 0.099
0.804HisTyr: 0.804 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
5.717IleAla: 5.717 ± 0.689
0.357IleCys: 0.357 ± 0.143
3.573IleAsp: 3.573 ± 0.736
4.288IleGlu: 4.288 ± 0.676
1.429IlePhe: 1.429 ± 0.336
3.305IleGly: 3.305 ± 0.514
1.429IleHis: 1.429 ± 0.382
2.055IleIle: 2.055 ± 0.406
2.591IleLys: 2.591 ± 0.505
3.484IleLeu: 3.484 ± 0.528
1.787IleMet: 1.787 ± 0.379
1.519IleAsn: 1.519 ± 0.349
3.216IlePro: 3.216 ± 0.794
3.127IleGln: 3.127 ± 0.664
2.323IleArg: 2.323 ± 0.507
3.931IleSer: 3.931 ± 0.647
4.556IleThr: 4.556 ± 0.89
2.501IleVal: 2.501 ± 0.536
0.447IleTrp: 0.447 ± 0.219
1.697IleTyr: 1.697 ± 0.431
0.0IleXaa: 0.0 ± 0.0
Lys
4.556LysAla: 4.556 ± 0.906
0.715LysCys: 0.715 ± 0.35
2.233LysAsp: 2.233 ± 0.519
3.663LysGlu: 3.663 ± 0.628
1.965LysPhe: 1.965 ± 0.37
2.68LysGly: 2.68 ± 0.489
0.536LysHis: 0.536 ± 0.223
2.501LysIle: 2.501 ± 0.372
2.948LysLys: 2.948 ± 0.662
5.539LysLeu: 5.539 ± 0.674
1.429LysMet: 1.429 ± 0.358
1.876LysAsn: 1.876 ± 0.41
3.573LysPro: 3.573 ± 0.779
2.323LysGln: 2.323 ± 0.531
2.948LysArg: 2.948 ± 0.582
3.841LysSer: 3.841 ± 0.565
2.859LysThr: 2.859 ± 0.522
2.68LysVal: 2.68 ± 0.562
1.251LysTrp: 1.251 ± 0.312
1.965LysTyr: 1.965 ± 0.512
0.0LysXaa: 0.0 ± 0.0
Leu
7.504LeuAla: 7.504 ± 0.799
0.804LeuCys: 0.804 ± 0.278
5.807LeuAsp: 5.807 ± 0.6
5.896LeuGlu: 5.896 ± 0.846
2.323LeuPhe: 2.323 ± 0.5
6.7LeuGly: 6.7 ± 0.759
1.787LeuHis: 1.787 ± 0.428
3.931LeuIle: 3.931 ± 0.538
4.467LeuLys: 4.467 ± 0.685
5.807LeuLeu: 5.807 ± 0.652
2.055LeuMet: 2.055 ± 0.483
3.752LeuAsn: 3.752 ± 0.573
3.037LeuPro: 3.037 ± 0.678
5.449LeuGln: 5.449 ± 0.74
4.288LeuArg: 4.288 ± 0.725
4.735LeuSer: 4.735 ± 0.635
3.931LeuThr: 3.931 ± 0.551
4.288LeuVal: 4.288 ± 0.605
0.625LeuTrp: 0.625 ± 0.287
2.233LeuTyr: 2.233 ± 0.539
0.0LeuXaa: 0.0 ± 0.0
Met
2.412MetAla: 2.412 ± 0.352
0.268MetCys: 0.268 ± 0.153
2.144MetAsp: 2.144 ± 0.545
1.161MetGlu: 1.161 ± 0.251
1.429MetPhe: 1.429 ± 0.343
1.519MetGly: 1.519 ± 0.441
0.357MetHis: 0.357 ± 0.25
1.429MetIle: 1.429 ± 0.49
0.893MetLys: 0.893 ± 0.207
1.876MetLeu: 1.876 ± 0.446
0.625MetMet: 0.625 ± 0.279
0.893MetAsn: 0.893 ± 0.224
1.429MetPro: 1.429 ± 0.315
1.608MetGln: 1.608 ± 0.409
2.412MetArg: 2.412 ± 0.423
2.591MetSer: 2.591 ± 0.438
2.323MetThr: 2.323 ± 0.481
1.072MetVal: 1.072 ± 0.3
0.268MetTrp: 0.268 ± 0.175
1.161MetTyr: 1.161 ± 0.334
0.0MetXaa: 0.0 ± 0.0
Asn
3.752AsnAla: 3.752 ± 0.665
0.268AsnCys: 0.268 ± 0.154
2.591AsnAsp: 2.591 ± 0.379
2.948AsnGlu: 2.948 ± 0.543
1.519AsnPhe: 1.519 ± 0.422
3.484AsnGly: 3.484 ± 0.56
1.251AsnHis: 1.251 ± 0.345
3.127AsnIle: 3.127 ± 0.568
2.055AsnLys: 2.055 ± 0.523
4.02AsnLeu: 4.02 ± 0.457
1.161AsnMet: 1.161 ± 0.296
2.233AsnAsn: 2.233 ± 0.511
2.68AsnPro: 2.68 ± 0.426
2.055AsnGln: 2.055 ± 0.551
2.769AsnArg: 2.769 ± 0.477
2.948AsnSer: 2.948 ± 0.531
3.127AsnThr: 3.127 ± 0.651
1.965AsnVal: 1.965 ± 0.405
0.536AsnTrp: 0.536 ± 0.207
1.697AsnTyr: 1.697 ± 0.347
0.0AsnXaa: 0.0 ± 0.0
Pro
2.859ProAla: 2.859 ± 0.407
0.179ProCys: 0.179 ± 0.129
2.323ProAsp: 2.323 ± 0.441
4.02ProGlu: 4.02 ± 0.642
1.965ProPhe: 1.965 ± 0.323
3.037ProGly: 3.037 ± 0.563
0.447ProHis: 0.447 ± 0.253
2.412ProIle: 2.412 ± 0.473
1.876ProLys: 1.876 ± 0.501
2.144ProLeu: 2.144 ± 0.382
1.161ProMet: 1.161 ± 0.346
2.68ProAsn: 2.68 ± 0.461
2.323ProPro: 2.323 ± 0.963
2.591ProGln: 2.591 ± 0.485
1.34ProArg: 1.34 ± 0.363
3.931ProSer: 3.931 ± 0.61
3.752ProThr: 3.752 ± 0.533
3.216ProVal: 3.216 ± 0.654
0.893ProTrp: 0.893 ± 0.251
1.697ProTyr: 1.697 ± 0.388
0.0ProXaa: 0.0 ± 0.0
Gln
5.896GlnAla: 5.896 ± 0.931
0.179GlnCys: 0.179 ± 0.119
1.34GlnAsp: 1.34 ± 0.324
2.769GlnGlu: 2.769 ± 0.541
2.233GlnPhe: 2.233 ± 0.363
3.395GlnGly: 3.395 ± 0.465
1.072GlnHis: 1.072 ± 0.348
2.591GlnIle: 2.591 ± 0.433
2.055GlnLys: 2.055 ± 0.475
5.092GlnLeu: 5.092 ± 0.854
1.34GlnMet: 1.34 ± 0.364
2.055GlnAsn: 2.055 ± 0.418
2.591GlnPro: 2.591 ± 0.555
3.216GlnGln: 3.216 ± 0.622
3.127GlnArg: 3.127 ± 0.712
2.591GlnSer: 2.591 ± 0.503
2.948GlnThr: 2.948 ± 0.451
1.787GlnVal: 1.787 ± 0.411
0.447GlnTrp: 0.447 ± 0.232
1.876GlnTyr: 1.876 ± 0.377
0.0GlnXaa: 0.0 ± 0.0
Arg
5.807ArgAla: 5.807 ± 0.609
0.268ArgCys: 0.268 ± 0.142
2.948ArgAsp: 2.948 ± 0.46
3.127ArgGlu: 3.127 ± 0.613
1.34ArgPhe: 1.34 ± 0.36
3.037ArgGly: 3.037 ± 0.509
0.893ArgHis: 0.893 ± 0.297
3.573ArgIle: 3.573 ± 0.806
3.216ArgLys: 3.216 ± 0.614
4.556ArgLeu: 4.556 ± 0.519
1.519ArgMet: 1.519 ± 0.269
2.68ArgAsn: 2.68 ± 0.428
1.34ArgPro: 1.34 ± 0.354
2.68ArgGln: 2.68 ± 0.589
2.412ArgArg: 2.412 ± 0.449
3.127ArgSer: 3.127 ± 0.443
3.127ArgThr: 3.127 ± 0.509
3.216ArgVal: 3.216 ± 0.464
0.268ArgTrp: 0.268 ± 0.138
1.519ArgTyr: 1.519 ± 0.314
0.0ArgXaa: 0.0 ± 0.0
Ser
6.164SerAla: 6.164 ± 0.67
0.625SerCys: 0.625 ± 0.306
3.663SerAsp: 3.663 ± 0.465
3.573SerGlu: 3.573 ± 0.537
2.501SerPhe: 2.501 ± 0.4
6.164SerGly: 6.164 ± 0.775
0.715SerHis: 0.715 ± 0.243
4.288SerIle: 4.288 ± 0.55
2.68SerLys: 2.68 ± 0.607
5.003SerLeu: 5.003 ± 0.669
1.608SerMet: 1.608 ± 0.36
4.02SerAsn: 4.02 ± 0.642
2.948SerPro: 2.948 ± 0.564
2.323SerGln: 2.323 ± 0.452
2.68SerArg: 2.68 ± 0.562
5.449SerSer: 5.449 ± 1.263
4.199SerThr: 4.199 ± 0.74
4.556SerVal: 4.556 ± 0.613
1.072SerTrp: 1.072 ± 0.306
2.233SerTyr: 2.233 ± 0.429
0.0SerXaa: 0.0 ± 0.0
Thr
6.343ThrAla: 6.343 ± 1.165
0.983ThrCys: 0.983 ± 0.344
4.02ThrAsp: 4.02 ± 0.539
3.395ThrGlu: 3.395 ± 0.591
2.144ThrPhe: 2.144 ± 0.387
5.271ThrGly: 5.271 ± 0.921
0.625ThrHis: 0.625 ± 0.225
3.841ThrIle: 3.841 ± 0.597
3.395ThrLys: 3.395 ± 0.482
4.288ThrLeu: 4.288 ± 0.626
1.161ThrMet: 1.161 ± 0.347
2.948ThrAsn: 2.948 ± 0.59
4.109ThrPro: 4.109 ± 0.537
2.412ThrGln: 2.412 ± 0.462
2.144ThrArg: 2.144 ± 0.343
5.003ThrSer: 5.003 ± 0.908
5.539ThrThr: 5.539 ± 1.104
5.181ThrVal: 5.181 ± 1.108
0.536ThrTrp: 0.536 ± 0.22
3.305ThrTyr: 3.305 ± 0.485
0.0ThrXaa: 0.0 ± 0.0
Val
5.985ValAla: 5.985 ± 0.899
1.072ValCys: 1.072 ± 0.337
3.841ValAsp: 3.841 ± 0.711
3.663ValGlu: 3.663 ± 0.508
2.144ValPhe: 2.144 ± 0.516
4.556ValGly: 4.556 ± 0.713
0.715ValHis: 0.715 ± 0.187
2.591ValIle: 2.591 ± 0.475
3.127ValLys: 3.127 ± 0.601
3.216ValLeu: 3.216 ± 0.493
2.144ValMet: 2.144 ± 0.421
2.591ValAsn: 2.591 ± 0.44
2.501ValPro: 2.501 ± 0.66
2.769ValGln: 2.769 ± 0.553
2.412ValArg: 2.412 ± 0.452
4.645ValSer: 4.645 ± 0.756
5.003ValThr: 5.003 ± 1.035
3.931ValVal: 3.931 ± 0.68
0.715ValTrp: 0.715 ± 0.289
1.787ValTyr: 1.787 ± 0.337
0.0ValXaa: 0.0 ± 0.0
Trp
0.625TrpAla: 0.625 ± 0.189
0.268TrpCys: 0.268 ± 0.165
1.072TrpAsp: 1.072 ± 0.313
1.34TrpGlu: 1.34 ± 0.416
0.357TrpPhe: 0.357 ± 0.186
0.357TrpGly: 0.357 ± 0.178
0.268TrpHis: 0.268 ± 0.137
0.536TrpIle: 0.536 ± 0.162
0.447TrpLys: 0.447 ± 0.248
1.34TrpLeu: 1.34 ± 0.305
0.357TrpMet: 0.357 ± 0.203
0.804TrpAsn: 0.804 ± 0.261
0.447TrpPro: 0.447 ± 0.15
0.268TrpGln: 0.268 ± 0.138
0.268TrpArg: 0.268 ± 0.16
1.161TrpSer: 1.161 ± 0.313
0.893TrpThr: 0.893 ± 0.251
1.072TrpVal: 1.072 ± 0.267
0.089TrpTrp: 0.089 ± 0.089
0.447TrpTyr: 0.447 ± 0.186
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.501TyrAla: 2.501 ± 0.442
0.536TyrCys: 0.536 ± 0.2
3.216TyrAsp: 3.216 ± 0.708
2.501TyrGlu: 2.501 ± 0.425
0.536TyrPhe: 0.536 ± 0.23
3.573TyrGly: 3.573 ± 0.511
0.357TyrHis: 0.357 ± 0.189
1.608TyrIle: 1.608 ± 0.437
2.859TyrLys: 2.859 ± 0.362
2.948TyrLeu: 2.948 ± 0.637
1.34TyrMet: 1.34 ± 0.382
2.501TyrAsn: 2.501 ± 0.499
1.072TyrPro: 1.072 ± 0.29
1.697TyrGln: 1.697 ± 0.392
1.876TyrArg: 1.876 ± 0.39
2.591TyrSer: 2.591 ± 0.552
2.323TyrThr: 2.323 ± 0.457
1.697TyrVal: 1.697 ± 0.361
0.268TyrTrp: 0.268 ± 0.142
1.34TyrTyr: 1.34 ± 0.294
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (11195 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski