Amino acid dipepetide frequency for Enterococcus phage EFDG1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.092AlaAla: 0.092 ± 0.05
0.621AlaCys: 0.621 ± 0.129
4.28AlaAsp: 4.28 ± 0.436
4.763AlaGlu: 4.763 ± 0.341
2.554AlaPhe: 2.554 ± 0.274
3.589AlaGly: 3.589 ± 0.463
1.196AlaHis: 1.196 ± 0.16
4.763AlaIle: 4.763 ± 0.366
5.085AlaLys: 5.085 ± 0.38
5.407AlaLeu: 5.407 ± 0.347
1.473AlaMet: 1.473 ± 0.211
3.405AlaAsn: 3.405 ± 0.365
2.416AlaPro: 2.416 ± 0.322
3.037AlaGln: 3.037 ± 0.322
2.968AlaArg: 2.968 ± 0.281
3.888AlaSer: 3.888 ± 0.425
4.441AlaThr: 4.441 ± 0.52
3.842AlaVal: 3.842 ± 0.277
0.575AlaTrp: 0.575 ± 0.106
2.968AlaTyr: 2.968 ± 0.243
0.0AlaXaa: 0.0 ± 0.0
Cys
0.414CysAla: 0.414 ± 0.12
0.092CysCys: 0.092 ± 0.045
0.598CysAsp: 0.598 ± 0.143
0.506CysGlu: 0.506 ± 0.093
0.345CysPhe: 0.345 ± 0.096
0.851CysGly: 0.851 ± 0.157
0.184CysHis: 0.184 ± 0.064
0.414CysIle: 0.414 ± 0.118
0.897CysLys: 0.897 ± 0.17
0.598CysLeu: 0.598 ± 0.13
0.138CysMet: 0.138 ± 0.056
0.253CysAsn: 0.253 ± 0.074
0.736CysPro: 0.736 ± 0.155
0.184CysGln: 0.184 ± 0.067
0.483CysArg: 0.483 ± 0.109
0.598CysSer: 0.598 ± 0.137
0.368CysThr: 0.368 ± 0.083
0.345CysVal: 0.345 ± 0.101
0.046CysTrp: 0.046 ± 0.031
0.621CysTyr: 0.621 ± 0.109
0.0CysXaa: 0.0 ± 0.0
Asp
3.359AspAla: 3.359 ± 0.27
0.506AspCys: 0.506 ± 0.123
3.129AspAsp: 3.129 ± 0.323
5.154AspGlu: 5.154 ± 0.432
2.646AspPhe: 2.646 ± 0.223
4.21AspGly: 4.21 ± 0.399
0.575AspHis: 0.575 ± 0.105
4.51AspIle: 4.51 ± 0.323
5.545AspLys: 5.545 ± 0.403
5.476AspLeu: 5.476 ± 0.327
2.025AspMet: 2.025 ± 0.233
3.106AspAsn: 3.106 ± 0.315
1.68AspPro: 1.68 ± 0.269
1.45AspGln: 1.45 ± 0.224
2.393AspArg: 2.393 ± 0.22
3.543AspSer: 3.543 ± 0.28
4.602AspThr: 4.602 ± 0.355
4.533AspVal: 4.533 ± 0.341
0.943AspTrp: 0.943 ± 0.135
4.118AspTyr: 4.118 ± 0.318
0.0AspXaa: 0.0 ± 0.0
Glu
5.614GluAla: 5.614 ± 0.362
0.736GluCys: 0.736 ± 0.146
5.062GluAsp: 5.062 ± 0.448
8.191GluGlu: 8.191 ± 0.654
2.761GluPhe: 2.761 ± 0.269
4.372GluGly: 4.372 ± 0.296
1.611GluHis: 1.611 ± 0.191
5.177GluIle: 5.177 ± 0.357
5.637GluLys: 5.637 ± 0.401
7.179GluLeu: 7.179 ± 0.486
2.071GluMet: 2.071 ± 0.205
4.349GluAsn: 4.349 ± 0.311
2.324GluPro: 2.324 ± 0.258
4.233GluGln: 4.233 ± 0.323
3.451GluArg: 3.451 ± 0.312
3.681GluSer: 3.681 ± 0.303
4.694GluThr: 4.694 ± 0.297
5.361GluVal: 5.361 ± 0.326
1.15GluTrp: 1.15 ± 0.167
3.313GluTyr: 3.313 ± 0.257
0.0GluXaa: 0.0 ± 0.0
Phe
2.209PheAla: 2.209 ± 0.202
0.368PheCys: 0.368 ± 0.085
2.554PheAsp: 2.554 ± 0.305
2.646PheGlu: 2.646 ± 0.276
1.127PhePhe: 1.127 ± 0.154
2.531PheGly: 2.531 ± 0.276
0.552PheHis: 0.552 ± 0.114
2.554PheIle: 2.554 ± 0.251
2.715PheLys: 2.715 ± 0.271
2.715PheLeu: 2.715 ± 0.298
1.035PheMet: 1.035 ± 0.164
2.531PheAsn: 2.531 ± 0.276
1.265PhePro: 1.265 ± 0.172
1.035PheGln: 1.035 ± 0.15
1.427PheArg: 1.427 ± 0.176
2.991PheSer: 2.991 ± 0.299
2.554PheThr: 2.554 ± 0.208
2.83PheVal: 2.83 ± 0.257
0.322PheTrp: 0.322 ± 0.089
1.956PheTyr: 1.956 ± 0.24
0.0PheXaa: 0.0 ± 0.0
Gly
3.773GlyAla: 3.773 ± 0.383
0.713GlyCys: 0.713 ± 0.15
3.658GlyAsp: 3.658 ± 0.32
4.671GlyGlu: 4.671 ± 0.307
2.715GlyPhe: 2.715 ± 0.276
4.671GlyGly: 4.671 ± 0.454
1.196GlyHis: 1.196 ± 0.174
4.533GlyIle: 4.533 ± 0.355
5.384GlyLys: 5.384 ± 0.339
4.395GlyLeu: 4.395 ± 0.331
1.519GlyMet: 1.519 ± 0.231
3.405GlyAsn: 3.405 ± 0.309
0.023GlyPro: 0.023 ± 0.021
2.209GlyGln: 2.209 ± 0.28
2.853GlyArg: 2.853 ± 0.317
3.865GlySer: 3.865 ± 0.366
4.947GlyThr: 4.947 ± 0.446
4.717GlyVal: 4.717 ± 0.323
0.92GlyTrp: 0.92 ± 0.148
3.221GlyTyr: 3.221 ± 0.264
0.0GlyXaa: 0.0 ± 0.0
His
0.874HisAla: 0.874 ± 0.128
0.115HisCys: 0.115 ± 0.05
0.782HisAsp: 0.782 ± 0.135
1.265HisGlu: 1.265 ± 0.192
0.828HisPhe: 0.828 ± 0.155
0.966HisGly: 0.966 ± 0.171
0.368HisHis: 0.368 ± 0.1
0.966HisIle: 0.966 ± 0.187
1.173HisLys: 1.173 ± 0.146
1.38HisLeu: 1.38 ± 0.158
0.414HisMet: 0.414 ± 0.096
0.874HisAsn: 0.874 ± 0.145
0.483HisPro: 0.483 ± 0.114
0.368HisGln: 0.368 ± 0.085
0.598HisArg: 0.598 ± 0.14
0.874HisSer: 0.874 ± 0.124
1.058HisThr: 1.058 ± 0.162
1.38HisVal: 1.38 ± 0.209
0.276HisTrp: 0.276 ± 0.091
1.173HisTyr: 1.173 ± 0.196
0.0HisXaa: 0.0 ± 0.0
Ile
4.256IleAla: 4.256 ± 0.311
0.621IleCys: 0.621 ± 0.121
4.671IleAsp: 4.671 ± 0.321
5.361IleGlu: 5.361 ± 0.422
1.979IlePhe: 1.979 ± 0.207
3.635IleGly: 3.635 ± 0.317
1.127IleHis: 1.127 ± 0.167
3.98IleIle: 3.98 ± 0.354
4.625IleLys: 4.625 ± 0.343
4.303IleLeu: 4.303 ± 0.36
1.749IleMet: 1.749 ± 0.221
3.612IleAsn: 3.612 ± 0.285
2.278IlePro: 2.278 ± 0.238
2.623IleGln: 2.623 ± 0.225
2.83IleArg: 2.83 ± 0.278
4.233IleSer: 4.233 ± 0.358
4.21IleThr: 4.21 ± 0.366
3.704IleVal: 3.704 ± 0.323
0.598IleTrp: 0.598 ± 0.101
2.347IleTyr: 2.347 ± 0.195
0.0IleXaa: 0.0 ± 0.0
Lys
5.246LysAla: 5.246 ± 0.387
0.736LysCys: 0.736 ± 0.147
5.085LysAsp: 5.085 ± 0.326
8.26LysGlu: 8.26 ± 0.523
2.209LysPhe: 2.209 ± 0.268
4.395LysGly: 4.395 ± 0.419
1.357LysHis: 1.357 ± 0.207
3.336LysIle: 3.336 ± 0.231
5.614LysLys: 5.614 ± 0.47
6.074LysLeu: 6.074 ± 0.314
2.347LysMet: 2.347 ± 0.249
3.681LysAsn: 3.681 ± 0.279
2.968LysPro: 2.968 ± 0.283
3.405LysGln: 3.405 ± 0.313
3.773LysArg: 3.773 ± 0.34
4.072LysSer: 4.072 ± 0.335
4.602LysThr: 4.602 ± 0.263
5.591LysVal: 5.591 ± 0.386
0.713LysTrp: 0.713 ± 0.13
3.29LysTyr: 3.29 ± 0.257
0.0LysXaa: 0.0 ± 0.0
Leu
5.108LeuAla: 5.108 ± 0.349
0.552LeuCys: 0.552 ± 0.105
6.005LeuAsp: 6.005 ± 0.402
6.35LeuGlu: 6.35 ± 0.4
3.037LeuPhe: 3.037 ± 0.297
5.568LeuGly: 5.568 ± 0.332
1.173LeuHis: 1.173 ± 0.19
4.694LeuIle: 4.694 ± 0.349
5.729LeuLys: 5.729 ± 0.281
6.304LeuLeu: 6.304 ± 0.365
1.841LeuMet: 1.841 ± 0.185
4.786LeuAsn: 4.786 ± 0.423
2.83LeuPro: 2.83 ± 0.274
3.52LeuGln: 3.52 ± 0.257
3.98LeuArg: 3.98 ± 0.3
4.855LeuSer: 4.855 ± 0.323
5.568LeuThr: 5.568 ± 0.341
5.361LeuVal: 5.361 ± 0.36
0.782LeuTrp: 0.782 ± 0.133
3.29LeuTyr: 3.29 ± 0.279
0.0LeuXaa: 0.0 ± 0.0
Met
1.749MetAla: 1.749 ± 0.181
0.276MetCys: 0.276 ± 0.096
1.15MetAsp: 1.15 ± 0.188
1.91MetGlu: 1.91 ± 0.189
0.897MetPhe: 0.897 ± 0.146
1.38MetGly: 1.38 ± 0.201
0.391MetHis: 0.391 ± 0.086
1.427MetIle: 1.427 ± 0.192
2.623MetLys: 2.623 ± 0.263
2.324MetLeu: 2.324 ± 0.209
0.552MetMet: 0.552 ± 0.096
1.657MetAsn: 1.657 ± 0.188
0.598MetPro: 0.598 ± 0.126
0.966MetGln: 0.966 ± 0.173
1.311MetArg: 1.311 ± 0.157
1.956MetSer: 1.956 ± 0.201
1.703MetThr: 1.703 ± 0.215
1.311MetVal: 1.311 ± 0.163
0.23MetTrp: 0.23 ± 0.066
1.45MetTyr: 1.45 ± 0.185
0.0MetXaa: 0.0 ± 0.0
Asn
3.198AsnAla: 3.198 ± 0.322
0.414AsnCys: 0.414 ± 0.094
2.669AsnAsp: 2.669 ± 0.239
3.681AsnGlu: 3.681 ± 0.342
1.818AsnPhe: 1.818 ± 0.227
4.141AsnGly: 4.141 ± 0.316
0.874AsnHis: 0.874 ± 0.149
3.083AsnIle: 3.083 ± 0.232
4.372AsnLys: 4.372 ± 0.287
4.187AsnLeu: 4.187 ± 0.328
1.565AsnMet: 1.565 ± 0.19
2.738AsnAsn: 2.738 ± 0.31
2.255AsnPro: 2.255 ± 0.205
1.772AsnGln: 1.772 ± 0.199
2.853AsnArg: 2.853 ± 0.305
3.382AsnSer: 3.382 ± 0.297
3.497AsnThr: 3.497 ± 0.369
3.635AsnVal: 3.635 ± 0.351
0.759AsnTrp: 0.759 ± 0.131
2.508AsnTyr: 2.508 ± 0.239
0.0AsnXaa: 0.0 ± 0.0
Pro
2.071ProAla: 2.071 ± 0.246
0.207ProCys: 0.207 ± 0.07
2.439ProAsp: 2.439 ± 0.252
2.945ProGlu: 2.945 ± 0.28
1.219ProPhe: 1.219 ± 0.18
0.575ProGly: 0.575 ± 0.129
0.529ProHis: 0.529 ± 0.12
1.864ProIle: 1.864 ± 0.2
2.669ProLys: 2.669 ± 0.241
2.531ProLeu: 2.531 ± 0.228
0.874ProMet: 0.874 ± 0.142
1.91ProAsn: 1.91 ± 0.245
0.69ProPro: 0.69 ± 0.134
1.104ProGln: 1.104 ± 0.231
1.265ProArg: 1.265 ± 0.16
2.37ProSer: 2.37 ± 0.278
2.048ProThr: 2.048 ± 0.239
2.945ProVal: 2.945 ± 0.369
0.322ProTrp: 0.322 ± 0.09
1.634ProTyr: 1.634 ± 0.201
0.0ProXaa: 0.0 ± 0.0
Gln
4.487GlnAla: 4.487 ± 0.481
0.23GlnCys: 0.23 ± 0.071
1.887GlnAsp: 1.887 ± 0.186
3.382GlnGlu: 3.382 ± 0.34
1.196GlnPhe: 1.196 ± 0.185
2.554GlnGly: 2.554 ± 0.237
0.506GlnHis: 0.506 ± 0.1
2.002GlnIle: 2.002 ± 0.207
2.646GlnLys: 2.646 ± 0.285
3.497GlnLeu: 3.497 ± 0.285
1.127GlnMet: 1.127 ± 0.195
1.403GlnAsn: 1.403 ± 0.152
1.265GlnPro: 1.265 ± 0.294
1.795GlnGln: 1.795 ± 0.346
1.565GlnArg: 1.565 ± 0.198
2.186GlnSer: 2.186 ± 0.22
1.91GlnThr: 1.91 ± 0.217
2.899GlnVal: 2.899 ± 0.274
0.184GlnTrp: 0.184 ± 0.069
1.887GlnTyr: 1.887 ± 0.171
0.0GlnXaa: 0.0 ± 0.0
Arg
2.623ArgAla: 2.623 ± 0.329
0.23ArgCys: 0.23 ± 0.078
2.83ArgAsp: 2.83 ± 0.24
3.681ArgGlu: 3.681 ± 0.326
1.864ArgPhe: 1.864 ± 0.207
2.669ArgGly: 2.669 ± 0.275
0.69ArgHis: 0.69 ± 0.137
3.221ArgIle: 3.221 ± 0.308
3.865ArgLys: 3.865 ± 0.336
4.349ArgLeu: 4.349 ± 0.326
1.265ArgMet: 1.265 ± 0.21
2.324ArgAsn: 2.324 ± 0.245
1.15ArgPro: 1.15 ± 0.182
1.588ArgGln: 1.588 ± 0.224
1.542ArgArg: 1.542 ± 0.171
1.864ArgSer: 1.864 ± 0.197
2.623ArgThr: 2.623 ± 0.256
3.129ArgVal: 3.129 ± 0.274
0.391ArgTrp: 0.391 ± 0.09
2.025ArgTyr: 2.025 ± 0.262
0.0ArgXaa: 0.0 ± 0.0
Ser
3.681SerAla: 3.681 ± 0.336
0.46SerCys: 0.46 ± 0.142
4.026SerAsp: 4.026 ± 0.32
3.888SerGlu: 3.888 ± 0.288
3.083SerPhe: 3.083 ± 0.276
4.901SerGly: 4.901 ± 0.412
0.736SerHis: 0.736 ± 0.15
4.026SerIle: 4.026 ± 0.248
4.671SerLys: 4.671 ± 0.365
5.016SerLeu: 5.016 ± 0.374
1.288SerMet: 1.288 ± 0.166
2.623SerAsn: 2.623 ± 0.257
2.002SerPro: 2.002 ± 0.221
1.841SerGln: 1.841 ± 0.234
2.439SerArg: 2.439 ± 0.246
3.796SerSer: 3.796 ± 0.361
3.658SerThr: 3.658 ± 0.305
3.796SerVal: 3.796 ± 0.256
0.782SerTrp: 0.782 ± 0.138
2.554SerTyr: 2.554 ± 0.248
0.0SerXaa: 0.0 ± 0.0
Thr
4.395ThrAla: 4.395 ± 0.447
0.46ThrCys: 0.46 ± 0.108
4.21ThrAsp: 4.21 ± 0.355
4.349ThrGlu: 4.349 ± 0.267
3.29ThrPhe: 3.29 ± 0.271
4.256ThrGly: 4.256 ± 0.333
1.081ThrHis: 1.081 ± 0.157
4.556ThrIle: 4.556 ± 0.346
4.049ThrLys: 4.049 ± 0.321
5.729ThrLeu: 5.729 ± 0.458
1.772ThrMet: 1.772 ± 0.19
3.359ThrAsn: 3.359 ± 0.307
3.014ThrPro: 3.014 ± 0.307
2.416ThrGln: 2.416 ± 0.264
2.393ThrArg: 2.393 ± 0.218
3.543ThrSer: 3.543 ± 0.306
3.98ThrThr: 3.98 ± 0.509
5.453ThrVal: 5.453 ± 0.607
0.851ThrTrp: 0.851 ± 0.156
2.531ThrTyr: 2.531 ± 0.351
0.0ThrXaa: 0.0 ± 0.0
Val
4.855ValAla: 4.855 ± 0.313
0.552ValCys: 0.552 ± 0.117
5.062ValAsp: 5.062 ± 0.303
5.66ValGlu: 5.66 ± 0.501
2.577ValPhe: 2.577 ± 0.256
4.395ValGly: 4.395 ± 0.366
1.127ValHis: 1.127 ± 0.179
4.164ValIle: 4.164 ± 0.268
5.2ValLys: 5.2 ± 0.397
5.269ValLeu: 5.269 ± 0.423
1.496ValMet: 1.496 ± 0.217
3.819ValAsn: 3.819 ± 0.301
2.347ValPro: 2.347 ± 0.215
2.669ValGln: 2.669 ± 0.278
3.152ValArg: 3.152 ± 0.264
4.095ValSer: 4.095 ± 0.306
5.2ValThr: 5.2 ± 0.549
4.993ValVal: 4.993 ± 0.404
0.598ValTrp: 0.598 ± 0.123
3.06ValTyr: 3.06 ± 0.269
0.0ValXaa: 0.0 ± 0.0
Trp
0.759TrpAla: 0.759 ± 0.141
0.161TrpCys: 0.161 ± 0.057
0.575TrpAsp: 0.575 ± 0.123
1.127TrpGlu: 1.127 ± 0.173
0.483TrpPhe: 0.483 ± 0.122
0.759TrpGly: 0.759 ± 0.117
0.138TrpHis: 0.138 ± 0.06
0.667TrpIle: 0.667 ± 0.121
0.736TrpLys: 0.736 ± 0.151
0.713TrpLeu: 0.713 ± 0.124
0.161TrpMet: 0.161 ± 0.056
0.552TrpAsn: 0.552 ± 0.11
0.0TrpPro: 0.0 ± 0.0
0.529TrpGln: 0.529 ± 0.116
0.46TrpArg: 0.46 ± 0.114
0.529TrpSer: 0.529 ± 0.101
0.92TrpThr: 0.92 ± 0.158
0.92TrpVal: 0.92 ± 0.191
0.23TrpTrp: 0.23 ± 0.09
0.713TrpTyr: 0.713 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.6TyrAla: 2.6 ± 0.257
0.621TyrCys: 0.621 ± 0.129
2.83TyrAsp: 2.83 ± 0.265
3.175TyrGlu: 3.175 ± 0.278
1.288TyrPhe: 1.288 ± 0.176
2.968TyrGly: 2.968 ± 0.272
0.805TyrHis: 0.805 ± 0.122
2.807TyrIle: 2.807 ± 0.274
3.52TyrLys: 3.52 ± 0.356
3.888TyrLeu: 3.888 ± 0.29
1.127TyrMet: 1.127 ± 0.155
3.037TyrAsn: 3.037 ± 0.249
1.887TyrPro: 1.887 ± 0.228
1.864TyrGln: 1.864 ± 0.223
2.186TyrArg: 2.186 ± 0.249
2.945TyrSer: 2.945 ± 0.279
3.106TyrThr: 3.106 ± 0.356
3.543TyrVal: 3.543 ± 0.253
0.46TyrTrp: 0.46 ± 0.102
2.094TyrTyr: 2.094 ± 0.259
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 192 proteins (43464 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski