Macleaya cordata

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Ranunculales; Papaveraceae; Papaveroideae; Macleaya

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21697 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A200Q3G1|A0A200Q3G1_9MAGN Cytochrome P450 OS=Macleaya cordata OX=56857 GN=BVC80_1211g57 PE=3 SV=1
MM1 pKa = 7.59EE2 pKa = 5.76NIKK5 pKa = 10.65DD6 pKa = 3.88GPGGAKK12 pKa = 9.89TNDD15 pKa = 3.75EE16 pKa = 4.46NPNSPSEE23 pKa = 4.25AEE25 pKa = 4.39TEE27 pKa = 4.33VEE29 pKa = 4.21TNLASSQQQSSRR41 pKa = 11.84TPFTNLSQIDD51 pKa = 3.81ADD53 pKa = 3.97LALARR58 pKa = 11.84TLQEE62 pKa = 3.58QEE64 pKa = 3.59RR65 pKa = 11.84AYY67 pKa = 11.04MMLRR71 pKa = 11.84MNGEE75 pKa = 3.75MSDD78 pKa = 3.84YY79 pKa = 10.85GSSDD83 pKa = 3.36SGSYY87 pKa = 10.3AYY89 pKa = 10.1EE90 pKa = 5.04DD91 pKa = 5.48DD92 pKa = 5.5VDD94 pKa = 6.32DD95 pKa = 7.03DD96 pKa = 5.65DD97 pKa = 7.39DD98 pKa = 5.14NDD100 pKa = 3.56NHH102 pKa = 6.4NVNNDD107 pKa = 3.32EE108 pKa = 4.01NSEE111 pKa = 3.94IDD113 pKa = 3.88GDD115 pKa = 3.93EE116 pKa = 3.8EE117 pKa = 4.92DD118 pKa = 4.59AFDD121 pKa = 3.87VHH123 pKa = 7.38AHH125 pKa = 6.48ADD127 pKa = 3.46EE128 pKa = 5.38DD129 pKa = 4.23NNQGAEE135 pKa = 4.38LDD137 pKa = 3.79PSAYY141 pKa = 10.57SSDD144 pKa = 3.4EE145 pKa = 3.93AYY147 pKa = 10.61ARR149 pKa = 11.84ALQEE153 pKa = 3.97AEE155 pKa = 4.08EE156 pKa = 5.1RR157 pKa = 11.84EE158 pKa = 3.9MAMRR162 pKa = 11.84LMALAGINDD171 pKa = 4.03FAVEE175 pKa = 4.02AEE177 pKa = 4.6DD178 pKa = 5.72NVDD181 pKa = 3.59HH182 pKa = 7.69DD183 pKa = 5.21SNSQVSYY190 pKa = 11.53EE191 pKa = 4.05EE192 pKa = 4.71LDD194 pKa = 3.73PDD196 pKa = 3.62EE197 pKa = 5.34LSYY200 pKa = 11.76EE201 pKa = 4.06EE202 pKa = 5.32LLALGEE208 pKa = 4.44VVGTEE213 pKa = 4.03SRR215 pKa = 11.84GLSSDD220 pKa = 4.16TIAALPSISYY230 pKa = 9.75KK231 pKa = 10.9AQDD234 pKa = 4.24DD235 pKa = 3.96QDD237 pKa = 4.02GSTDD241 pKa = 3.07QCVICRR247 pKa = 11.84LEE249 pKa = 4.21YY250 pKa = 10.83DD251 pKa = 3.8DD252 pKa = 6.65GEE254 pKa = 4.57TLTMLSCKK262 pKa = 10.0HH263 pKa = 6.45SYY265 pKa = 10.38HH266 pKa = 7.05PEE268 pKa = 4.54CINNWLQINKK278 pKa = 8.6VCPVCSTEE286 pKa = 3.92VSTSEE291 pKa = 4.3SSRR294 pKa = 3.48

Molecular weight: 32.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A200QLK3|A0A200QLK3_9MAGN DNA-binding WRKY OS=Macleaya cordata OX=56857 GN=BVC80_9007g24 PE=4 SV=1
MM1 pKa = 7.71ASHH4 pKa = 6.87SPLNVSNISNLVPIKK19 pKa = 9.84LTRR22 pKa = 11.84EE23 pKa = 3.76NYY25 pKa = 9.84ILWKK29 pKa = 10.62SVFLPILRR37 pKa = 11.84SYY39 pKa = 11.34NLLRR43 pKa = 11.84FVDD46 pKa = 5.23GSHH49 pKa = 7.26PCPSRR54 pKa = 11.84LVTDD58 pKa = 4.19AAGKK62 pKa = 8.08DD63 pKa = 3.71TQATNPDD70 pKa = 3.28FTEE73 pKa = 3.67WRR75 pKa = 11.84QLLTAEE81 pKa = 4.7APPTAFSASTGPRR94 pKa = 11.84TFTRR98 pKa = 11.84APSNQQSTARR108 pKa = 11.84FSKK111 pKa = 10.33DD112 pKa = 2.57KK113 pKa = 10.75HH114 pKa = 5.39KK115 pKa = 11.31ARR117 pKa = 11.84VFYY120 pKa = 10.51RR121 pKa = 11.84GSSSNSGSGSGSGSGSSPRR140 pKa = 11.84TSDD143 pKa = 3.81RR144 pKa = 11.84PVCQICNRR152 pKa = 11.84VGHH155 pKa = 5.56TAIDD159 pKa = 3.36CHH161 pKa = 7.33NRR163 pKa = 11.84MNHH166 pKa = 5.94AFQGRR171 pKa = 11.84IPTQRR176 pKa = 11.84LRR178 pKa = 11.84AMVATNAIHH187 pKa = 6.84ARR189 pKa = 11.84PSLGTEE195 pKa = 4.15GLVTLPLPRR204 pKa = 11.84SSCYY208 pKa = 10.5EE209 pKa = 4.04LVTKK213 pKa = 10.67

Molecular weight: 23.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21697

0

21697

9115806

34

5524

420.1

46.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.386 ± 0.015

1.812 ± 0.008

5.287 ± 0.012

6.576 ± 0.018

4.23 ± 0.011

6.616 ± 0.014

2.344 ± 0.006

5.479 ± 0.013

6.09 ± 0.015

9.622 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.007

4.494 ± 0.01

4.9 ± 0.016

3.683 ± 0.014

5.327 ± 0.014

9.088 ± 0.02

4.984 ± 0.01

6.539 ± 0.011

1.332 ± 0.006

2.758 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski