Amino acid dipepetide frequency for Escherichia phage RCS47

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.801AlaAla: 10.801 ± 0.837
1.047AlaCys: 1.047 ± 0.193
5.744AlaAsp: 5.744 ± 0.455
6.881AlaGlu: 6.881 ± 0.463
3.53AlaPhe: 3.53 ± 0.336
6.612AlaGly: 6.612 ± 0.555
1.466AlaHis: 1.466 ± 0.237
6.193AlaIle: 6.193 ± 0.443
4.907AlaLys: 4.907 ± 0.416
9.664AlaLeu: 9.664 ± 0.583
2.364AlaMet: 2.364 ± 0.238
4.069AlaAsn: 4.069 ± 0.446
3.082AlaPro: 3.082 ± 0.345
3.949AlaGln: 3.949 ± 0.389
6.253AlaArg: 6.253 ± 0.528
5.296AlaSer: 5.296 ± 0.376
5.445AlaThr: 5.445 ± 0.396
5.894AlaVal: 5.894 ± 0.46
1.257AlaTrp: 1.257 ± 0.174
2.543AlaTyr: 2.543 ± 0.325
0.0AlaXaa: 0.0 ± 0.0
Cys
0.688CysAla: 0.688 ± 0.113
0.299CysCys: 0.299 ± 0.099
0.688CysAsp: 0.688 ± 0.153
0.509CysGlu: 0.509 ± 0.118
0.479CysPhe: 0.479 ± 0.131
1.257CysGly: 1.257 ± 0.204
0.359CysHis: 0.359 ± 0.12
0.688CysIle: 0.688 ± 0.14
0.688CysLys: 0.688 ± 0.165
0.868CysLeu: 0.868 ± 0.149
0.18CysMet: 0.18 ± 0.077
0.359CysAsn: 0.359 ± 0.11
0.718CysPro: 0.718 ± 0.188
0.568CysGln: 0.568 ± 0.177
0.838CysArg: 0.838 ± 0.165
0.898CysSer: 0.898 ± 0.191
0.479CysThr: 0.479 ± 0.12
0.748CysVal: 0.748 ± 0.166
0.15CysTrp: 0.15 ± 0.089
0.568CysTyr: 0.568 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
5.475AspAla: 5.475 ± 0.458
0.539AspCys: 0.539 ± 0.12
3.979AspAsp: 3.979 ± 0.438
4.039AspGlu: 4.039 ± 0.415
2.064AspPhe: 2.064 ± 0.22
4.847AspGly: 4.847 ± 0.335
0.957AspHis: 0.957 ± 0.221
3.321AspIle: 3.321 ± 0.325
2.812AspLys: 2.812 ± 0.271
4.458AspLeu: 4.458 ± 0.36
2.094AspMet: 2.094 ± 0.279
2.334AspAsn: 2.334 ± 0.318
2.064AspPro: 2.064 ± 0.242
1.646AspGln: 1.646 ± 0.229
2.962AspArg: 2.962 ± 0.307
4.428AspSer: 4.428 ± 0.36
3.411AspThr: 3.411 ± 0.437
3.8AspVal: 3.8 ± 0.327
0.718AspTrp: 0.718 ± 0.14
1.945AspTyr: 1.945 ± 0.234
0.0AspXaa: 0.0 ± 0.0
Glu
6.044GluAla: 6.044 ± 0.474
0.568GluCys: 0.568 ± 0.121
3.411GluAsp: 3.411 ± 0.354
4.248GluGlu: 4.248 ± 0.36
2.483GluPhe: 2.483 ± 0.298
3.53GluGly: 3.53 ± 0.333
1.287GluHis: 1.287 ± 0.212
3.83GluIle: 3.83 ± 0.376
3.8GluLys: 3.8 ± 0.411
6.851GluLeu: 6.851 ± 0.499
2.633GluMet: 2.633 ± 0.295
2.932GluAsn: 2.932 ± 0.325
2.064GluPro: 2.064 ± 0.304
3.5GluGln: 3.5 ± 0.366
4.398GluArg: 4.398 ± 0.529
3.201GluSer: 3.201 ± 0.341
3.291GluThr: 3.291 ± 0.337
3.919GluVal: 3.919 ± 0.321
1.107GluTrp: 1.107 ± 0.204
2.064GluTyr: 2.064 ± 0.298
0.0GluXaa: 0.0 ± 0.0
Phe
2.393PheAla: 2.393 ± 0.211
0.389PheCys: 0.389 ± 0.111
2.423PheAsp: 2.423 ± 0.247
1.885PheGlu: 1.885 ± 0.178
1.436PhePhe: 1.436 ± 0.197
2.364PheGly: 2.364 ± 0.312
0.598PheHis: 0.598 ± 0.116
2.513PheIle: 2.513 ± 0.308
2.005PheLys: 2.005 ± 0.255
2.543PheLeu: 2.543 ± 0.358
0.778PheMet: 0.778 ± 0.142
1.945PheAsn: 1.945 ± 0.19
1.436PhePro: 1.436 ± 0.242
1.197PheGln: 1.197 ± 0.204
2.034PheArg: 2.034 ± 0.24
2.453PheSer: 2.453 ± 0.266
2.244PheThr: 2.244 ± 0.271
2.364PheVal: 2.364 ± 0.276
0.329PheTrp: 0.329 ± 0.11
1.257PheTyr: 1.257 ± 0.255
0.0PheXaa: 0.0 ± 0.0
Gly
5.595GlyAla: 5.595 ± 0.561
0.987GlyCys: 0.987 ± 0.23
4.338GlyAsp: 4.338 ± 0.45
4.069GlyGlu: 4.069 ± 0.289
2.393GlyPhe: 2.393 ± 0.203
5.056GlyGly: 5.056 ± 0.57
1.227GlyHis: 1.227 ± 0.173
4.039GlyIle: 4.039 ± 0.344
4.847GlyLys: 4.847 ± 0.412
5.116GlyLeu: 5.116 ± 0.372
1.975GlyMet: 1.975 ± 0.229
3.65GlyAsn: 3.65 ± 0.463
1.466GlyPro: 1.466 ± 0.236
2.603GlyGln: 2.603 ± 0.224
4.099GlyArg: 4.099 ± 0.391
3.65GlySer: 3.65 ± 0.399
4.009GlyThr: 4.009 ± 0.462
5.565GlyVal: 5.565 ± 0.45
1.197GlyTrp: 1.197 ± 0.224
2.364GlyTyr: 2.364 ± 0.291
0.0GlyXaa: 0.0 ± 0.0
His
1.616HisAla: 1.616 ± 0.205
0.359HisCys: 0.359 ± 0.109
0.838HisAsp: 0.838 ± 0.169
0.838HisGlu: 0.838 ± 0.155
0.778HisPhe: 0.778 ± 0.15
1.526HisGly: 1.526 ± 0.202
0.479HisHis: 0.479 ± 0.125
1.047HisIle: 1.047 ± 0.178
1.017HisLys: 1.017 ± 0.2
1.885HisLeu: 1.885 ± 0.243
0.299HisMet: 0.299 ± 0.091
0.778HisAsn: 0.778 ± 0.159
1.017HisPro: 1.017 ± 0.172
0.957HisGln: 0.957 ± 0.168
1.346HisArg: 1.346 ± 0.196
1.436HisSer: 1.436 ± 0.189
0.898HisThr: 0.898 ± 0.173
0.957HisVal: 0.957 ± 0.174
0.269HisTrp: 0.269 ± 0.075
0.688HisTyr: 0.688 ± 0.155
0.0HisXaa: 0.0 ± 0.0
Ile
5.894IleAla: 5.894 ± 0.426
0.838IleCys: 0.838 ± 0.134
3.86IleAsp: 3.86 ± 0.37
4.248IleGlu: 4.248 ± 0.392
1.675IlePhe: 1.675 ± 0.207
3.83IleGly: 3.83 ± 0.375
0.778IleHis: 0.778 ± 0.144
2.782IleIle: 2.782 ± 0.271
2.992IleLys: 2.992 ± 0.277
4.338IleLeu: 4.338 ± 0.392
1.077IleMet: 1.077 ± 0.185
3.321IleAsn: 3.321 ± 0.34
2.663IlePro: 2.663 ± 0.305
2.154IleGln: 2.154 ± 0.256
3.291IleArg: 3.291 ± 0.316
4.817IleSer: 4.817 ± 0.385
4.308IleThr: 4.308 ± 0.393
2.812IleVal: 2.812 ± 0.299
0.479IleTrp: 0.479 ± 0.125
1.675IleTyr: 1.675 ± 0.231
0.0IleXaa: 0.0 ± 0.0
Lys
6.163LysAla: 6.163 ± 0.553
0.269LysCys: 0.269 ± 0.103
2.902LysAsp: 2.902 ± 0.269
4.189LysGlu: 4.189 ± 0.369
1.646LysPhe: 1.646 ± 0.234
3.441LysGly: 3.441 ± 0.348
1.227LysHis: 1.227 ± 0.186
2.812LysIle: 2.812 ± 0.342
3.59LysLys: 3.59 ± 0.4
4.578LysLeu: 4.578 ± 0.368
1.556LysMet: 1.556 ± 0.197
2.753LysAsn: 2.753 ± 0.313
2.124LysPro: 2.124 ± 0.24
2.453LysGln: 2.453 ± 0.28
3.022LysArg: 3.022 ± 0.325
3.919LysSer: 3.919 ± 0.358
3.471LysThr: 3.471 ± 0.252
3.5LysVal: 3.5 ± 0.407
0.838LysTrp: 0.838 ± 0.155
1.855LysTyr: 1.855 ± 0.252
0.0LysXaa: 0.0 ± 0.0
Leu
9.664LeuAla: 9.664 ± 0.65
1.376LeuCys: 1.376 ± 0.246
5.056LeuAsp: 5.056 ± 0.342
6.014LeuGlu: 6.014 ± 0.455
2.782LeuPhe: 2.782 ± 0.325
4.817LeuGly: 4.817 ± 0.383
1.855LeuHis: 1.855 ± 0.205
3.86LeuIle: 3.86 ± 0.347
5.535LeuLys: 5.535 ± 0.446
7.36LeuLeu: 7.36 ± 0.545
2.364LeuMet: 2.364 ± 0.278
4.518LeuAsn: 4.518 ± 0.288
4.428LeuPro: 4.428 ± 0.447
3.83LeuGln: 3.83 ± 0.367
5.565LeuArg: 5.565 ± 0.471
6.014LeuSer: 6.014 ± 0.36
4.966LeuThr: 4.966 ± 0.373
5.176LeuVal: 5.176 ± 0.337
0.898LeuTrp: 0.898 ± 0.155
1.945LeuTyr: 1.945 ± 0.231
0.0LeuXaa: 0.0 ± 0.0
Met
2.812MetAla: 2.812 ± 0.328
0.329MetCys: 0.329 ± 0.107
1.316MetAsp: 1.316 ± 0.229
1.137MetGlu: 1.137 ± 0.197
0.748MetPhe: 0.748 ± 0.142
1.496MetGly: 1.496 ± 0.198
0.479MetHis: 0.479 ± 0.137
1.556MetIle: 1.556 ± 0.208
1.705MetLys: 1.705 ± 0.228
2.543MetLeu: 2.543 ± 0.245
0.868MetMet: 0.868 ± 0.164
1.227MetAsn: 1.227 ± 0.181
1.137MetPro: 1.137 ± 0.175
1.107MetGln: 1.107 ± 0.158
1.915MetArg: 1.915 ± 0.221
2.005MetSer: 2.005 ± 0.245
1.915MetThr: 1.915 ± 0.234
1.137MetVal: 1.137 ± 0.147
0.239MetTrp: 0.239 ± 0.076
0.359MetTyr: 0.359 ± 0.128
0.0MetXaa: 0.0 ± 0.0
Asn
4.428AsnAla: 4.428 ± 0.406
0.359AsnCys: 0.359 ± 0.099
2.094AsnAsp: 2.094 ± 0.259
3.052AsnGlu: 3.052 ± 0.287
1.017AsnPhe: 1.017 ± 0.185
4.338AsnGly: 4.338 ± 0.434
0.808AsnHis: 0.808 ± 0.149
2.872AsnIle: 2.872 ± 0.318
2.723AsnLys: 2.723 ± 0.264
3.65AsnLeu: 3.65 ± 0.361
0.868AsnMet: 0.868 ± 0.129
2.094AsnAsn: 2.094 ± 0.263
2.064AsnPro: 2.064 ± 0.311
2.034AsnGln: 2.034 ± 0.205
2.244AsnArg: 2.244 ± 0.26
3.141AsnSer: 3.141 ± 0.319
2.244AsnThr: 2.244 ± 0.282
2.782AsnVal: 2.782 ± 0.3
0.628AsnTrp: 0.628 ± 0.135
1.316AsnTyr: 1.316 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
3.919ProAla: 3.919 ± 0.361
0.509ProCys: 0.509 ± 0.141
2.753ProAsp: 2.753 ± 0.302
3.65ProGlu: 3.65 ± 0.315
1.735ProPhe: 1.735 ± 0.244
2.902ProGly: 2.902 ± 0.305
0.449ProHis: 0.449 ± 0.119
1.855ProIle: 1.855 ± 0.228
1.646ProLys: 1.646 ± 0.238
3.291ProLeu: 3.291 ± 0.275
0.688ProMet: 0.688 ± 0.146
1.496ProAsn: 1.496 ± 0.198
1.466ProPro: 1.466 ± 0.234
1.526ProGln: 1.526 ± 0.217
1.616ProArg: 1.616 ± 0.257
2.364ProSer: 2.364 ± 0.275
2.364ProThr: 2.364 ± 0.271
3.56ProVal: 3.56 ± 0.338
0.568ProTrp: 0.568 ± 0.159
1.376ProTyr: 1.376 ± 0.203
0.0ProXaa: 0.0 ± 0.0
Gln
4.009GlnAla: 4.009 ± 0.391
0.509GlnCys: 0.509 ± 0.158
1.705GlnAsp: 1.705 ± 0.22
2.573GlnGlu: 2.573 ± 0.281
1.466GlnPhe: 1.466 ± 0.245
1.885GlnGly: 1.885 ± 0.248
1.257GlnHis: 1.257 ± 0.203
2.304GlnIle: 2.304 ± 0.242
2.483GlnLys: 2.483 ± 0.363
4.248GlnLeu: 4.248 ± 0.35
1.406GlnMet: 1.406 ± 0.19
1.915GlnAsn: 1.915 ± 0.224
1.436GlnPro: 1.436 ± 0.215
2.782GlnGln: 2.782 ± 0.292
3.052GlnArg: 3.052 ± 0.293
2.214GlnSer: 2.214 ± 0.295
2.154GlnThr: 2.154 ± 0.264
2.543GlnVal: 2.543 ± 0.258
0.718GlnTrp: 0.718 ± 0.162
1.616GlnTyr: 1.616 ± 0.232
0.0GlnXaa: 0.0 ± 0.0
Arg
5.834ArgAla: 5.834 ± 0.405
0.598ArgCys: 0.598 ± 0.15
3.231ArgAsp: 3.231 ± 0.292
3.949ArgGlu: 3.949 ± 0.34
2.274ArgPhe: 2.274 ± 0.263
3.201ArgGly: 3.201 ± 0.347
1.586ArgHis: 1.586 ± 0.267
4.338ArgIle: 4.338 ± 0.368
4.637ArgLys: 4.637 ± 0.43
5.924ArgLeu: 5.924 ± 0.426
1.526ArgMet: 1.526 ± 0.227
2.364ArgAsn: 2.364 ± 0.254
2.274ArgPro: 2.274 ± 0.268
2.483ArgGln: 2.483 ± 0.316
4.488ArgArg: 4.488 ± 0.412
2.962ArgSer: 2.962 ± 0.279
2.842ArgThr: 2.842 ± 0.276
3.86ArgVal: 3.86 ± 0.31
0.987ArgTrp: 0.987 ± 0.214
2.064ArgTyr: 2.064 ± 0.241
0.0ArgXaa: 0.0 ± 0.0
Ser
5.655SerAla: 5.655 ± 0.483
1.107SerCys: 1.107 ± 0.212
4.159SerAsp: 4.159 ± 0.327
3.74SerGlu: 3.74 ± 0.307
1.885SerPhe: 1.885 ± 0.284
5.744SerGly: 5.744 ± 0.416
1.137SerHis: 1.137 ± 0.214
4.099SerIle: 4.099 ± 0.265
3.082SerLys: 3.082 ± 0.309
5.505SerLeu: 5.505 ± 0.433
1.496SerMet: 1.496 ± 0.227
2.154SerAsn: 2.154 ± 0.285
2.573SerPro: 2.573 ± 0.257
2.723SerGln: 2.723 ± 0.281
3.949SerArg: 3.949 ± 0.335
3.8SerSer: 3.8 ± 0.353
3.68SerThr: 3.68 ± 0.32
4.697SerVal: 4.697 ± 0.362
0.957SerTrp: 0.957 ± 0.179
1.765SerTyr: 1.765 ± 0.262
0.0SerXaa: 0.0 ± 0.0
Thr
6.223ThrAla: 6.223 ± 0.664
0.509ThrCys: 0.509 ± 0.148
3.321ThrAsp: 3.321 ± 0.313
3.5ThrGlu: 3.5 ± 0.31
2.184ThrPhe: 2.184 ± 0.318
4.667ThrGly: 4.667 ± 0.482
1.107ThrHis: 1.107 ± 0.168
3.5ThrIle: 3.5 ± 0.339
2.483ThrLys: 2.483 ± 0.285
5.415ThrLeu: 5.415 ± 0.355
1.137ThrMet: 1.137 ± 0.172
1.885ThrAsn: 1.885 ± 0.278
3.351ThrPro: 3.351 ± 0.301
2.094ThrGln: 2.094 ± 0.268
3.112ThrArg: 3.112 ± 0.298
3.68ThrSer: 3.68 ± 0.405
3.59ThrThr: 3.59 ± 0.486
3.77ThrVal: 3.77 ± 0.361
0.628ThrTrp: 0.628 ± 0.171
1.945ThrTyr: 1.945 ± 0.279
0.0ThrXaa: 0.0 ± 0.0
Val
6.014ValAla: 6.014 ± 0.414
0.688ValCys: 0.688 ± 0.191
4.039ValAsp: 4.039 ± 0.408
3.8ValGlu: 3.8 ± 0.29
2.094ValPhe: 2.094 ± 0.252
3.889ValGly: 3.889 ± 0.28
0.898ValHis: 0.898 ± 0.158
3.83ValIle: 3.83 ± 0.316
3.71ValLys: 3.71 ± 0.277
5.385ValLeu: 5.385 ± 0.464
1.406ValMet: 1.406 ± 0.203
3.171ValAsn: 3.171 ± 0.282
2.573ValPro: 2.573 ± 0.267
2.304ValGln: 2.304 ± 0.283
3.86ValArg: 3.86 ± 0.344
5.296ValSer: 5.296 ± 0.431
4.368ValThr: 4.368 ± 0.394
4.278ValVal: 4.278 ± 0.4
0.748ValTrp: 0.748 ± 0.17
1.705ValTyr: 1.705 ± 0.217
0.0ValXaa: 0.0 ± 0.0
Trp
1.257TrpAla: 1.257 ± 0.212
0.209TrpCys: 0.209 ± 0.083
0.539TrpAsp: 0.539 ± 0.12
0.658TrpGlu: 0.658 ± 0.138
0.658TrpPhe: 0.658 ± 0.124
0.658TrpGly: 0.658 ± 0.153
0.449TrpHis: 0.449 ± 0.108
0.898TrpIle: 0.898 ± 0.217
0.419TrpLys: 0.419 ± 0.095
1.795TrpLeu: 1.795 ± 0.296
0.359TrpMet: 0.359 ± 0.111
0.479TrpAsn: 0.479 ± 0.139
0.389TrpPro: 0.389 ± 0.099
0.718TrpGln: 0.718 ± 0.108
0.987TrpArg: 0.987 ± 0.176
0.778TrpSer: 0.778 ± 0.208
0.748TrpThr: 0.748 ± 0.161
0.718TrpVal: 0.718 ± 0.16
0.209TrpTrp: 0.209 ± 0.067
0.359TrpTyr: 0.359 ± 0.12
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.513TyrAla: 2.513 ± 0.238
0.539TyrCys: 0.539 ± 0.107
1.496TyrAsp: 1.496 ± 0.193
2.184TyrGlu: 2.184 ± 0.247
1.257TyrPhe: 1.257 ± 0.222
2.214TyrGly: 2.214 ± 0.292
0.628TyrHis: 0.628 ± 0.11
1.496TyrIle: 1.496 ± 0.179
1.227TyrLys: 1.227 ± 0.198
2.603TyrLeu: 2.603 ± 0.327
0.927TyrMet: 0.927 ± 0.175
1.316TyrAsn: 1.316 ± 0.145
1.406TyrPro: 1.406 ± 0.18
1.646TyrGln: 1.646 ± 0.226
2.393TyrArg: 2.393 ± 0.273
1.556TyrSer: 1.556 ± 0.208
1.675TyrThr: 1.675 ± 0.254
1.975TyrVal: 1.975 ± 0.244
0.359TyrTrp: 0.359 ± 0.096
0.927TyrTyr: 0.927 ± 0.188
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 122 proteins (33425 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski