Amino acid dipepetide frequency for Gordonia phage GAL1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.821AlaAla: 14.821 ± 1.16
0.946AlaCys: 0.946 ± 0.271
7.505AlaAsp: 7.505 ± 0.608
7.442AlaGlu: 7.442 ± 0.771
2.838AlaPhe: 2.838 ± 0.401
9.082AlaGly: 9.082 ± 1.134
1.892AlaHis: 1.892 ± 0.435
4.352AlaIle: 4.352 ± 0.453
4.604AlaLys: 4.604 ± 0.536
7.568AlaLeu: 7.568 ± 0.949
3.153AlaMet: 3.153 ± 0.463
3.343AlaAsn: 3.343 ± 0.428
5.108AlaPro: 5.108 ± 0.658
5.424AlaGln: 5.424 ± 1.074
9.334AlaArg: 9.334 ± 0.987
5.928AlaSer: 5.928 ± 0.684
6.244AlaThr: 6.244 ± 0.571
7.694AlaVal: 7.694 ± 0.816
2.27AlaTrp: 2.27 ± 0.358
2.334AlaTyr: 2.334 ± 0.404
0.0AlaXaa: 0.0 ± 0.0
Cys
1.072CysAla: 1.072 ± 0.339
0.252CysCys: 0.252 ± 0.141
0.82CysAsp: 0.82 ± 0.252
0.82CysGlu: 0.82 ± 0.247
0.315CysPhe: 0.315 ± 0.149
1.261CysGly: 1.261 ± 0.356
0.441CysHis: 0.441 ± 0.179
0.631CysIle: 0.631 ± 0.203
0.189CysLys: 0.189 ± 0.129
0.757CysLeu: 0.757 ± 0.195
0.126CysMet: 0.126 ± 0.098
0.505CysAsn: 0.505 ± 0.17
0.757CysPro: 0.757 ± 0.219
0.252CysGln: 0.252 ± 0.124
1.135CysArg: 1.135 ± 0.308
0.631CysSer: 0.631 ± 0.33
0.568CysThr: 0.568 ± 0.197
0.694CysVal: 0.694 ± 0.249
0.189CysTrp: 0.189 ± 0.109
0.315CysTyr: 0.315 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
7.947AspAla: 7.947 ± 0.572
0.505AspCys: 0.505 ± 0.175
5.045AspAsp: 5.045 ± 0.633
3.973AspGlu: 3.973 ± 0.582
1.955AspPhe: 1.955 ± 0.336
5.613AspGly: 5.613 ± 0.643
1.451AspHis: 1.451 ± 0.323
3.153AspIle: 3.153 ± 0.515
1.64AspLys: 1.64 ± 0.327
6.37AspLeu: 6.37 ± 0.551
0.82AspMet: 0.82 ± 0.187
1.703AspAsn: 1.703 ± 0.411
4.226AspPro: 4.226 ± 0.611
2.018AspGln: 2.018 ± 0.301
5.108AspArg: 5.108 ± 0.628
2.712AspSer: 2.712 ± 0.416
4.162AspThr: 4.162 ± 0.497
5.676AspVal: 5.676 ± 0.544
1.387AspTrp: 1.387 ± 0.289
1.387AspTyr: 1.387 ± 0.323
0.0AspXaa: 0.0 ± 0.0
Glu
5.928GluAla: 5.928 ± 0.535
0.441GluCys: 0.441 ± 0.186
3.28GluAsp: 3.28 ± 0.445
2.964GluGlu: 2.964 ± 0.566
1.766GluPhe: 1.766 ± 0.337
3.658GluGly: 3.658 ± 0.469
1.324GluHis: 1.324 ± 0.374
2.334GluIle: 2.334 ± 0.385
1.955GluLys: 1.955 ± 0.399
6.433GluLeu: 6.433 ± 0.744
1.135GluMet: 1.135 ± 0.277
1.135GluAsn: 1.135 ± 0.231
2.901GluPro: 2.901 ± 0.371
2.397GluGln: 2.397 ± 0.458
5.676GluArg: 5.676 ± 0.829
3.28GluSer: 3.28 ± 0.466
2.901GluThr: 2.901 ± 0.371
3.973GluVal: 3.973 ± 0.704
1.324GluTrp: 1.324 ± 0.301
1.703GluTyr: 1.703 ± 0.352
0.0GluXaa: 0.0 ± 0.0
Phe
2.397PheAla: 2.397 ± 0.427
0.315PheCys: 0.315 ± 0.151
2.775PheAsp: 2.775 ± 0.617
1.451PheGlu: 1.451 ± 0.355
0.568PhePhe: 0.568 ± 0.267
2.838PheGly: 2.838 ± 0.419
0.694PheHis: 0.694 ± 0.195
1.577PheIle: 1.577 ± 0.387
1.009PheLys: 1.009 ± 0.244
1.955PheLeu: 1.955 ± 0.361
0.568PheMet: 0.568 ± 0.177
0.505PheAsn: 0.505 ± 0.169
1.514PhePro: 1.514 ± 0.324
1.324PheGln: 1.324 ± 0.27
2.081PheArg: 2.081 ± 0.458
1.766PheSer: 1.766 ± 0.399
2.649PheThr: 2.649 ± 0.399
1.64PheVal: 1.64 ± 0.329
0.441PheTrp: 0.441 ± 0.159
0.757PheTyr: 0.757 ± 0.238
0.0PheXaa: 0.0 ± 0.0
Gly
7.631GlyAla: 7.631 ± 0.844
1.009GlyCys: 1.009 ± 0.263
5.172GlyAsp: 5.172 ± 0.752
4.099GlyGlu: 4.099 ± 0.447
3.469GlyPhe: 3.469 ± 0.574
7.19GlyGly: 7.19 ± 0.571
1.577GlyHis: 1.577 ± 0.294
3.595GlyIle: 3.595 ± 0.596
2.964GlyLys: 2.964 ± 0.553
5.613GlyLeu: 5.613 ± 0.562
1.64GlyMet: 1.64 ± 0.266
2.27GlyAsn: 2.27 ± 0.401
5.108GlyPro: 5.108 ± 0.58
3.532GlyGln: 3.532 ± 0.555
5.487GlyArg: 5.487 ± 0.685
5.361GlySer: 5.361 ± 0.473
6.054GlyThr: 6.054 ± 0.705
5.928GlyVal: 5.928 ± 0.737
1.703GlyTrp: 1.703 ± 0.289
2.46GlyTyr: 2.46 ± 0.424
0.0GlyXaa: 0.0 ± 0.0
His
2.144HisAla: 2.144 ± 0.41
0.252HisCys: 0.252 ± 0.128
1.009HisAsp: 1.009 ± 0.258
1.135HisGlu: 1.135 ± 0.293
0.063HisPhe: 0.063 ± 0.064
2.081HisGly: 2.081 ± 0.446
0.82HisHis: 0.82 ± 0.258
1.324HisIle: 1.324 ± 0.34
0.505HisLys: 0.505 ± 0.16
1.64HisLeu: 1.64 ± 0.327
0.568HisMet: 0.568 ± 0.196
0.694HisAsn: 0.694 ± 0.232
1.451HisPro: 1.451 ± 0.35
0.82HisGln: 0.82 ± 0.254
1.64HisArg: 1.64 ± 0.356
1.261HisSer: 1.261 ± 0.295
1.766HisThr: 1.766 ± 0.384
2.018HisVal: 2.018 ± 0.384
0.757HisTrp: 0.757 ± 0.185
0.505HisTyr: 0.505 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
5.928IleAla: 5.928 ± 0.717
0.441IleCys: 0.441 ± 0.161
3.784IleAsp: 3.784 ± 0.44
3.406IleGlu: 3.406 ± 0.432
0.252IlePhe: 0.252 ± 0.109
3.153IleGly: 3.153 ± 0.388
1.261IleHis: 1.261 ± 0.317
1.514IleIle: 1.514 ± 0.247
1.198IleLys: 1.198 ± 0.23
3.469IleLeu: 3.469 ± 0.54
0.883IleMet: 0.883 ± 0.263
1.451IleAsn: 1.451 ± 0.414
2.144IlePro: 2.144 ± 0.335
1.451IleGln: 1.451 ± 0.254
2.838IleArg: 2.838 ± 0.372
1.829IleSer: 1.829 ± 0.36
2.901IleThr: 2.901 ± 0.409
2.964IleVal: 2.964 ± 0.416
0.946IleTrp: 0.946 ± 0.196
0.757IleTyr: 0.757 ± 0.235
0.0IleXaa: 0.0 ± 0.0
Lys
3.784LysAla: 3.784 ± 0.453
0.252LysCys: 0.252 ± 0.151
1.829LysAsp: 1.829 ± 0.325
1.324LysGlu: 1.324 ± 0.279
1.135LysPhe: 1.135 ± 0.301
2.46LysGly: 2.46 ± 0.423
1.324LysHis: 1.324 ± 0.371
1.577LysIle: 1.577 ± 0.305
1.766LysLys: 1.766 ± 0.395
2.712LysLeu: 2.712 ± 0.528
0.441LysMet: 0.441 ± 0.179
0.568LysAsn: 0.568 ± 0.148
1.829LysPro: 1.829 ± 0.331
0.883LysGln: 0.883 ± 0.237
2.081LysArg: 2.081 ± 0.47
1.577LysSer: 1.577 ± 0.331
1.766LysThr: 1.766 ± 0.344
2.838LysVal: 2.838 ± 0.494
0.883LysTrp: 0.883 ± 0.254
1.387LysTyr: 1.387 ± 0.236
0.0LysXaa: 0.0 ± 0.0
Leu
8.766LeuAla: 8.766 ± 0.856
0.82LeuCys: 0.82 ± 0.22
5.108LeuAsp: 5.108 ± 0.492
4.478LeuGlu: 4.478 ± 0.523
2.207LeuPhe: 2.207 ± 0.384
6.244LeuGly: 6.244 ± 1.048
1.955LeuHis: 1.955 ± 0.412
3.343LeuIle: 3.343 ± 0.48
2.46LeuLys: 2.46 ± 0.346
6.685LeuLeu: 6.685 ± 0.917
1.577LeuMet: 1.577 ± 0.282
2.46LeuAsn: 2.46 ± 0.39
4.478LeuPro: 4.478 ± 0.548
2.207LeuGln: 2.207 ± 0.375
6.811LeuArg: 6.811 ± 0.61
4.352LeuSer: 4.352 ± 0.66
5.739LeuThr: 5.739 ± 0.69
6.244LeuVal: 6.244 ± 0.717
1.261LeuTrp: 1.261 ± 0.268
1.387LeuTyr: 1.387 ± 0.256
0.0LeuXaa: 0.0 ± 0.0
Met
2.649MetAla: 2.649 ± 0.396
0.189MetCys: 0.189 ± 0.112
1.261MetAsp: 1.261 ± 0.279
0.631MetGlu: 0.631 ± 0.235
0.757MetPhe: 0.757 ± 0.189
1.514MetGly: 1.514 ± 0.378
0.315MetHis: 0.315 ± 0.135
0.82MetIle: 0.82 ± 0.26
0.441MetLys: 0.441 ± 0.188
1.324MetLeu: 1.324 ± 0.235
0.568MetMet: 0.568 ± 0.204
0.946MetAsn: 0.946 ± 0.223
1.703MetPro: 1.703 ± 0.433
1.261MetGln: 1.261 ± 0.381
1.577MetArg: 1.577 ± 0.289
2.144MetSer: 2.144 ± 0.348
2.081MetThr: 2.081 ± 0.463
1.135MetVal: 1.135 ± 0.21
0.505MetTrp: 0.505 ± 0.158
0.252MetTyr: 0.252 ± 0.102
0.0MetXaa: 0.0 ± 0.0
Asn
3.973AsnAla: 3.973 ± 0.496
0.441AsnCys: 0.441 ± 0.178
1.261AsnAsp: 1.261 ± 0.281
1.577AsnGlu: 1.577 ± 0.409
0.505AsnPhe: 0.505 ± 0.186
3.027AsnGly: 3.027 ± 0.42
0.883AsnHis: 0.883 ± 0.225
0.694AsnIle: 0.694 ± 0.221
0.946AsnLys: 0.946 ± 0.29
2.207AsnLeu: 2.207 ± 0.33
0.378AsnMet: 0.378 ± 0.149
1.009AsnAsn: 1.009 ± 0.241
3.027AsnPro: 3.027 ± 0.46
1.135AsnGln: 1.135 ± 0.26
2.46AsnArg: 2.46 ± 0.41
0.883AsnSer: 0.883 ± 0.203
2.144AsnThr: 2.144 ± 0.352
1.955AsnVal: 1.955 ± 0.328
0.441AsnTrp: 0.441 ± 0.167
0.694AsnTyr: 0.694 ± 0.214
0.0AsnXaa: 0.0 ± 0.0
Pro
5.991ProAla: 5.991 ± 0.714
0.631ProCys: 0.631 ± 0.205
4.856ProAsp: 4.856 ± 0.698
3.532ProGlu: 3.532 ± 0.46
1.703ProPhe: 1.703 ± 0.348
5.802ProGly: 5.802 ± 0.648
1.324ProHis: 1.324 ± 0.203
2.144ProIle: 2.144 ± 0.368
2.144ProLys: 2.144 ± 0.43
4.226ProLeu: 4.226 ± 0.498
1.577ProMet: 1.577 ± 0.34
1.955ProAsn: 1.955 ± 0.357
3.027ProPro: 3.027 ± 0.537
2.523ProGln: 2.523 ± 0.428
3.406ProArg: 3.406 ± 0.455
3.595ProSer: 3.595 ± 0.448
3.406ProThr: 3.406 ± 0.44
5.172ProVal: 5.172 ± 0.42
1.135ProTrp: 1.135 ± 0.267
1.514ProTyr: 1.514 ± 0.274
0.0ProXaa: 0.0 ± 0.0
Gln
4.856GlnAla: 4.856 ± 0.648
0.378GlnCys: 0.378 ± 0.146
1.892GlnAsp: 1.892 ± 0.287
2.081GlnGlu: 2.081 ± 0.406
1.072GlnPhe: 1.072 ± 0.23
2.712GlnGly: 2.712 ± 0.392
0.82GlnHis: 0.82 ± 0.213
1.324GlnIle: 1.324 ± 0.245
1.198GlnLys: 1.198 ± 0.244
3.09GlnLeu: 3.09 ± 0.638
1.135GlnMet: 1.135 ± 0.292
0.82GlnAsn: 0.82 ± 0.25
2.144GlnPro: 2.144 ± 0.327
2.46GlnGln: 2.46 ± 0.536
3.532GlnArg: 3.532 ± 0.44
1.955GlnSer: 1.955 ± 0.424
2.775GlnThr: 2.775 ± 0.397
2.586GlnVal: 2.586 ± 0.38
1.009GlnTrp: 1.009 ± 0.272
0.631GlnTyr: 0.631 ± 0.212
0.0GlnXaa: 0.0 ± 0.0
Arg
8.073ArgAla: 8.073 ± 0.879
1.64ArgCys: 1.64 ± 0.333
5.235ArgAsp: 5.235 ± 0.554
4.036ArgGlu: 4.036 ± 0.524
2.523ArgPhe: 2.523 ± 0.451
3.595ArgGly: 3.595 ± 0.483
1.64ArgHis: 1.64 ± 0.377
3.973ArgIle: 3.973 ± 0.482
3.406ArgLys: 3.406 ± 0.535
5.613ArgLeu: 5.613 ± 0.423
2.523ArgMet: 2.523 ± 0.373
2.523ArgAsn: 2.523 ± 0.507
4.793ArgPro: 4.793 ± 0.652
2.144ArgGln: 2.144 ± 0.366
6.874ArgArg: 6.874 ± 0.853
4.289ArgSer: 4.289 ± 0.562
4.982ArgThr: 4.982 ± 0.611
5.424ArgVal: 5.424 ± 0.837
1.009ArgTrp: 1.009 ± 0.216
2.397ArgTyr: 2.397 ± 0.413
0.0ArgXaa: 0.0 ± 0.0
Ser
5.424SerAla: 5.424 ± 0.764
0.378SerCys: 0.378 ± 0.188
3.784SerAsp: 3.784 ± 0.386
3.721SerGlu: 3.721 ± 0.562
2.712SerPhe: 2.712 ± 0.378
5.172SerGly: 5.172 ± 0.739
1.009SerHis: 1.009 ± 0.29
1.829SerIle: 1.829 ± 0.338
1.703SerLys: 1.703 ± 0.316
3.91SerLeu: 3.91 ± 0.543
1.514SerMet: 1.514 ± 0.306
1.829SerAsn: 1.829 ± 0.38
3.216SerPro: 3.216 ± 0.461
1.766SerGln: 1.766 ± 0.283
3.216SerArg: 3.216 ± 0.549
3.153SerSer: 3.153 ± 0.582
4.478SerThr: 4.478 ± 0.572
4.226SerVal: 4.226 ± 0.445
0.946SerTrp: 0.946 ± 0.21
1.261SerTyr: 1.261 ± 0.267
0.0SerXaa: 0.0 ± 0.0
Thr
8.01ThrAla: 8.01 ± 0.681
0.694ThrCys: 0.694 ± 0.206
4.856ThrAsp: 4.856 ± 0.702
2.838ThrGlu: 2.838 ± 0.384
1.955ThrPhe: 1.955 ± 0.353
6.937ThrGly: 6.937 ± 0.713
1.577ThrHis: 1.577 ± 0.346
3.09ThrIle: 3.09 ± 0.459
1.387ThrLys: 1.387 ± 0.314
5.487ThrLeu: 5.487 ± 0.553
0.694ThrMet: 0.694 ± 0.197
2.334ThrAsn: 2.334 ± 0.388
5.361ThrPro: 5.361 ± 0.612
2.018ThrGln: 2.018 ± 0.383
4.226ThrArg: 4.226 ± 0.52
4.541ThrSer: 4.541 ± 0.487
6.181ThrThr: 6.181 ± 0.776
4.036ThrVal: 4.036 ± 0.535
0.568ThrTrp: 0.568 ± 0.193
1.64ThrTyr: 1.64 ± 0.271
0.0ThrXaa: 0.0 ± 0.0
Val
7.064ValAla: 7.064 ± 0.585
1.261ValCys: 1.261 ± 0.362
4.793ValAsp: 4.793 ± 0.625
4.793ValGlu: 4.793 ± 0.568
1.64ValPhe: 1.64 ± 0.277
5.802ValGly: 5.802 ± 0.682
1.261ValHis: 1.261 ± 0.222
3.784ValIle: 3.784 ± 0.501
1.766ValLys: 1.766 ± 0.283
5.487ValLeu: 5.487 ± 0.525
1.766ValMet: 1.766 ± 0.383
2.775ValAsn: 2.775 ± 0.627
4.793ValPro: 4.793 ± 0.59
3.406ValGln: 3.406 ± 0.553
5.802ValArg: 5.802 ± 0.766
3.721ValSer: 3.721 ± 0.503
4.982ValThr: 4.982 ± 0.622
5.298ValVal: 5.298 ± 0.574
1.766ValTrp: 1.766 ± 0.487
1.198ValTyr: 1.198 ± 0.257
0.0ValXaa: 0.0 ± 0.0
Trp
2.46TrpAla: 2.46 ± 0.428
0.378TrpCys: 0.378 ± 0.151
0.82TrpAsp: 0.82 ± 0.215
0.883TrpGlu: 0.883 ± 0.274
0.694TrpPhe: 0.694 ± 0.174
1.387TrpGly: 1.387 ± 0.266
0.505TrpHis: 0.505 ± 0.181
0.757TrpIle: 0.757 ± 0.213
0.378TrpLys: 0.378 ± 0.16
2.27TrpLeu: 2.27 ± 0.393
0.694TrpMet: 0.694 ± 0.205
0.631TrpAsn: 0.631 ± 0.222
0.568TrpPro: 0.568 ± 0.181
0.631TrpGln: 0.631 ± 0.165
1.703TrpArg: 1.703 ± 0.325
1.135TrpSer: 1.135 ± 0.251
1.324TrpThr: 1.324 ± 0.352
1.387TrpVal: 1.387 ± 0.273
0.946TrpTrp: 0.946 ± 0.285
0.252TrpTyr: 0.252 ± 0.106
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.964TyrAla: 2.964 ± 0.418
0.505TyrCys: 0.505 ± 0.276
1.766TyrAsp: 1.766 ± 0.276
1.324TyrGlu: 1.324 ± 0.229
0.694TyrPhe: 0.694 ± 0.179
2.334TyrGly: 2.334 ± 0.366
0.378TyrHis: 0.378 ± 0.136
0.757TyrIle: 0.757 ± 0.187
0.757TyrLys: 0.757 ± 0.221
1.766TyrLeu: 1.766 ± 0.338
0.252TyrMet: 0.252 ± 0.113
0.189TyrAsn: 0.189 ± 0.104
1.514TyrPro: 1.514 ± 0.301
0.82TyrGln: 0.82 ± 0.23
1.703TyrArg: 1.703 ± 0.385
1.198TyrSer: 1.198 ± 0.29
1.324TyrThr: 1.324 ± 0.261
2.27TyrVal: 2.27 ± 0.32
0.315TyrTrp: 0.315 ± 0.135
0.315TyrTyr: 0.315 ± 0.128
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (15857 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski