Bacillus phage Novomoskovsk

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Andromedavirus; unclassified Andromedavirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9Z8U5|A0A6M9Z8U5_9CAUD Uncharacterized protein OS=Bacillus phage Novomoskovsk OX=2736258 GN=Novomoskovsk_6 PE=4 SV=1
MM1 pKa = 7.62SILKK5 pKa = 8.23EE6 pKa = 3.94FCDD9 pKa = 3.53KK10 pKa = 10.74VRR12 pKa = 11.84EE13 pKa = 4.13LEE15 pKa = 4.1EE16 pKa = 4.3EE17 pKa = 4.16YY18 pKa = 11.06GVYY21 pKa = 10.62LDD23 pKa = 4.69VDD25 pKa = 4.19PDD27 pKa = 3.63QIIIVAVNNEE37 pKa = 4.15DD38 pKa = 3.55GQEE41 pKa = 3.38RR42 pKa = 11.84WIYY45 pKa = 11.02DD46 pKa = 3.49DD47 pKa = 3.76EE48 pKa = 4.45

Molecular weight: 5.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9Z6C7|A0A6M9Z6C7_9CAUD FtsK/SpoIIIE-family protein OS=Bacillus phage Novomoskovsk OX=2736258 GN=Novomoskovsk_68 PE=4 SV=1
MM1 pKa = 7.45SLLKK5 pKa = 10.64SILKK9 pKa = 10.59SPTTKK14 pKa = 10.13KK15 pKa = 10.07VAIRR19 pKa = 11.84GIKK22 pKa = 9.87VGLPVLLRR30 pKa = 11.84YY31 pKa = 8.73AQKK34 pKa = 10.76KK35 pKa = 9.97RR36 pKa = 11.84GVKK39 pKa = 9.78

Molecular weight: 4.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

14738

38

1026

184.2

20.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.202 ± 0.447

0.916 ± 0.142

6.208 ± 0.282

8.135 ± 0.488

4.607 ± 0.211

6.683 ± 0.345

2.124 ± 0.219

6.853 ± 0.201

8.115 ± 0.5

7.783 ± 0.407

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.816 ± 0.183

4.865 ± 0.34

3.243 ± 0.149

3.474 ± 0.182

4.383 ± 0.239

6.256 ± 0.359

6.154 ± 0.427

6.1 ± 0.255

1.126 ± 0.108

3.956 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski