Amino acid dipepetide frequency for Bacillus phage Novomoskovsk

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.646AlaAla: 2.646 ± 0.728
0.611AlaCys: 0.611 ± 0.22
3.529AlaAsp: 3.529 ± 0.489
3.596AlaGlu: 3.596 ± 0.469
3.325AlaPhe: 3.325 ± 0.483
4.343AlaGly: 4.343 ± 0.688
1.086AlaHis: 1.086 ± 0.299
5.632AlaIle: 5.632 ± 0.773
5.7AlaLys: 5.7 ± 0.708
4.954AlaLeu: 4.954 ± 0.778
1.764AlaMet: 1.764 ± 0.364
2.375AlaAsn: 2.375 ± 0.353
2.511AlaPro: 2.511 ± 0.452
1.764AlaGln: 1.764 ± 0.337
3.121AlaArg: 3.121 ± 0.4
3.461AlaSer: 3.461 ± 0.577
3.664AlaThr: 3.664 ± 0.609
4.071AlaVal: 4.071 ± 0.754
0.882AlaTrp: 0.882 ± 0.263
2.986AlaTyr: 2.986 ± 0.302
0.0AlaXaa: 0.0 ± 0.0
Cys
0.679CysAla: 0.679 ± 0.245
0.068CysCys: 0.068 ± 0.06
0.611CysAsp: 0.611 ± 0.222
0.407CysGlu: 0.407 ± 0.156
0.814CysPhe: 0.814 ± 0.243
0.814CysGly: 0.814 ± 0.216
0.475CysHis: 0.475 ± 0.189
0.271CysIle: 0.271 ± 0.106
1.154CysLys: 1.154 ± 0.291
1.086CysLeu: 1.086 ± 0.312
0.136CysMet: 0.136 ± 0.101
0.271CysAsn: 0.271 ± 0.138
0.204CysPro: 0.204 ± 0.116
0.204CysGln: 0.204 ± 0.126
0.204CysArg: 0.204 ± 0.122
0.475CysSer: 0.475 ± 0.17
0.746CysThr: 0.746 ± 0.244
0.204CysVal: 0.204 ± 0.109
0.068CysTrp: 0.068 ± 0.069
0.271CysTyr: 0.271 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
3.461AspAla: 3.461 ± 0.39
0.543AspCys: 0.543 ± 0.2
3.936AspAsp: 3.936 ± 0.516
4.071AspGlu: 4.071 ± 0.654
3.8AspPhe: 3.8 ± 0.604
3.868AspGly: 3.868 ± 0.479
1.968AspHis: 1.968 ± 0.442
4.546AspIle: 4.546 ± 0.563
5.496AspLys: 5.496 ± 0.662
4.954AspLeu: 4.954 ± 0.444
1.9AspMet: 1.9 ± 0.31
2.307AspAsn: 2.307 ± 0.531
2.714AspPro: 2.714 ± 0.448
2.239AspGln: 2.239 ± 0.396
3.325AspArg: 3.325 ± 0.414
3.596AspSer: 3.596 ± 0.468
2.511AspThr: 2.511 ± 0.518
3.393AspVal: 3.393 ± 0.378
0.814AspTrp: 0.814 ± 0.223
2.646AspTyr: 2.646 ± 0.408
0.0AspXaa: 0.0 ± 0.0
Glu
5.496GluAla: 5.496 ± 0.778
0.679GluCys: 0.679 ± 0.203
4.546GluAsp: 4.546 ± 0.615
8.753GluGlu: 8.753 ± 1.346
3.461GluPhe: 3.461 ± 0.488
4.954GluGly: 4.954 ± 0.66
1.764GluHis: 1.764 ± 0.402
4.886GluIle: 4.886 ± 0.544
4.411GluLys: 4.411 ± 0.561
7.803GluLeu: 7.803 ± 0.79
2.307GluMet: 2.307 ± 0.436
4.071GluAsn: 4.071 ± 0.552
2.104GluPro: 2.104 ± 0.389
4.071GluGln: 4.071 ± 0.704
3.732GluArg: 3.732 ± 0.519
3.8GluSer: 3.8 ± 0.533
4.411GluThr: 4.411 ± 0.681
4.818GluVal: 4.818 ± 0.645
0.95GluTrp: 0.95 ± 0.22
4.343GluTyr: 4.343 ± 0.64
0.0GluXaa: 0.0 ± 0.0
Phe
2.85PheAla: 2.85 ± 0.657
0.407PheCys: 0.407 ± 0.138
3.393PheAsp: 3.393 ± 0.577
4.479PheGlu: 4.479 ± 0.511
2.036PhePhe: 2.036 ± 0.414
3.189PheGly: 3.189 ± 0.402
0.95PheHis: 0.95 ± 0.292
2.375PheIle: 2.375 ± 0.404
3.325PheLys: 3.325 ± 0.572
3.461PheLeu: 3.461 ± 0.471
1.629PheMet: 1.629 ± 0.377
2.036PheAsn: 2.036 ± 0.32
1.832PhePro: 1.832 ± 0.327
1.086PheGln: 1.086 ± 0.264
1.696PheArg: 1.696 ± 0.413
2.646PheSer: 2.646 ± 0.424
4.004PheThr: 4.004 ± 0.575
2.511PheVal: 2.511 ± 0.408
0.271PheTrp: 0.271 ± 0.13
1.9PheTyr: 1.9 ± 0.373
0.0PheXaa: 0.0 ± 0.0
Gly
5.021GlyAla: 5.021 ± 0.645
0.611GlyCys: 0.611 ± 0.22
4.682GlyAsp: 4.682 ± 0.539
4.614GlyGlu: 4.614 ± 0.531
3.393GlyPhe: 3.393 ± 0.435
4.682GlyGly: 4.682 ± 0.721
1.289GlyHis: 1.289 ± 0.341
4.479GlyIle: 4.479 ± 0.505
5.768GlyLys: 5.768 ± 0.663
5.496GlyLeu: 5.496 ± 0.62
2.036GlyMet: 2.036 ± 0.455
3.054GlyAsn: 3.054 ± 0.775
0.136GlyPro: 0.136 ± 0.119
2.375GlyGln: 2.375 ± 0.397
2.714GlyArg: 2.714 ± 0.41
4.614GlySer: 4.614 ± 0.831
2.986GlyThr: 2.986 ± 0.541
4.954GlyVal: 4.954 ± 0.764
0.814GlyTrp: 0.814 ± 0.204
3.054GlyTyr: 3.054 ± 0.593
0.0GlyXaa: 0.0 ± 0.0
His
1.018HisAla: 1.018 ± 0.259
0.271HisCys: 0.271 ± 0.162
0.882HisAsp: 0.882 ± 0.235
1.357HisGlu: 1.357 ± 0.344
1.425HisPhe: 1.425 ± 0.33
1.968HisGly: 1.968 ± 0.416
0.611HisHis: 0.611 ± 0.194
1.493HisIle: 1.493 ± 0.351
1.493HisLys: 1.493 ± 0.353
1.018HisLeu: 1.018 ± 0.226
0.611HisMet: 0.611 ± 0.169
1.221HisAsn: 1.221 ± 0.239
1.289HisPro: 1.289 ± 0.298
0.814HisGln: 0.814 ± 0.26
0.814HisArg: 0.814 ± 0.189
1.493HisSer: 1.493 ± 0.327
0.882HisThr: 0.882 ± 0.278
1.018HisVal: 1.018 ± 0.275
0.407HisTrp: 0.407 ± 0.141
1.154HisTyr: 1.154 ± 0.314
0.0HisXaa: 0.0 ± 0.0
Ile
4.071IleAla: 4.071 ± 0.536
0.611IleCys: 0.611 ± 0.203
5.429IleAsp: 5.429 ± 0.685
5.7IleGlu: 5.7 ± 0.63
2.239IlePhe: 2.239 ± 0.426
3.868IleGly: 3.868 ± 0.556
1.425IleHis: 1.425 ± 0.288
3.732IleIle: 3.732 ± 0.548
6.582IleLys: 6.582 ± 0.706
4.479IleLeu: 4.479 ± 0.566
1.832IleMet: 1.832 ± 0.361
3.529IleAsn: 3.529 ± 0.401
3.121IlePro: 3.121 ± 0.44
2.511IleGln: 2.511 ± 0.364
2.85IleArg: 2.85 ± 0.405
3.936IleSer: 3.936 ± 0.518
5.021IleThr: 5.021 ± 0.573
4.886IleVal: 4.886 ± 0.51
0.407IleTrp: 0.407 ± 0.171
2.307IleTyr: 2.307 ± 0.592
0.0IleXaa: 0.0 ± 0.0
Lys
4.343LysAla: 4.343 ± 0.536
0.543LysCys: 0.543 ± 0.239
4.886LysAsp: 4.886 ± 0.636
8.618LysGlu: 8.618 ± 1.096
3.732LysPhe: 3.732 ± 0.65
4.886LysGly: 4.886 ± 0.928
2.036LysHis: 2.036 ± 0.443
4.071LysIle: 4.071 ± 0.598
7.736LysLys: 7.736 ± 1.006
5.768LysLeu: 5.768 ± 0.718
2.579LysMet: 2.579 ± 0.502
3.936LysAsn: 3.936 ± 0.446
2.918LysPro: 2.918 ± 0.416
3.393LysGln: 3.393 ± 0.469
3.936LysArg: 3.936 ± 0.633
4.886LysSer: 4.886 ± 0.775
4.207LysThr: 4.207 ± 0.54
5.361LysVal: 5.361 ± 0.836
0.611LysTrp: 0.611 ± 0.189
2.714LysTyr: 2.714 ± 0.383
0.0LysXaa: 0.0 ± 0.0
Leu
4.275LeuAla: 4.275 ± 0.636
0.882LeuCys: 0.882 ± 0.239
5.089LeuAsp: 5.089 ± 0.549
6.243LeuGlu: 6.243 ± 0.804
2.579LeuPhe: 2.579 ± 0.376
5.089LeuGly: 5.089 ± 0.587
1.425LeuHis: 1.425 ± 0.319
5.496LeuIle: 5.496 ± 0.628
7.057LeuLys: 7.057 ± 0.851
5.564LeuLeu: 5.564 ± 0.718
2.307LeuMet: 2.307 ± 0.37
4.071LeuAsn: 4.071 ± 0.562
3.189LeuPro: 3.189 ± 0.381
2.579LeuGln: 2.579 ± 0.495
2.782LeuArg: 2.782 ± 0.391
5.429LeuSer: 5.429 ± 0.614
5.293LeuThr: 5.293 ± 0.588
5.021LeuVal: 5.021 ± 0.553
0.611LeuTrp: 0.611 ± 0.197
2.85LeuTyr: 2.85 ± 0.505
0.0LeuXaa: 0.0 ± 0.0
Met
2.307MetAla: 2.307 ± 0.417
0.271MetCys: 0.271 ± 0.113
1.629MetAsp: 1.629 ± 0.36
1.968MetGlu: 1.968 ± 0.298
1.357MetPhe: 1.357 ± 0.314
1.832MetGly: 1.832 ± 0.409
0.814MetHis: 0.814 ± 0.271
2.036MetIle: 2.036 ± 0.365
2.646MetLys: 2.646 ± 0.432
1.629MetLeu: 1.629 ± 0.382
0.679MetMet: 0.679 ± 0.198
1.221MetAsn: 1.221 ± 0.267
0.814MetPro: 0.814 ± 0.281
0.611MetGln: 0.611 ± 0.2
0.882MetArg: 0.882 ± 0.242
3.054MetSer: 3.054 ± 0.442
2.104MetThr: 2.104 ± 0.382
0.95MetVal: 0.95 ± 0.259
0.339MetTrp: 0.339 ± 0.173
1.018MetTyr: 1.018 ± 0.262
0.0MetXaa: 0.0 ± 0.0
Asn
2.511AsnAla: 2.511 ± 0.508
0.475AsnCys: 0.475 ± 0.179
2.918AsnAsp: 2.918 ± 0.327
3.461AsnGlu: 3.461 ± 0.466
1.9AsnPhe: 1.9 ± 0.323
4.479AsnGly: 4.479 ± 0.542
0.95AsnHis: 0.95 ± 0.274
3.257AsnIle: 3.257 ± 0.567
3.936AsnLys: 3.936 ± 0.506
3.529AsnLeu: 3.529 ± 0.468
1.425AsnMet: 1.425 ± 0.302
3.257AsnAsn: 3.257 ± 0.475
1.561AsnPro: 1.561 ± 0.336
1.696AsnGln: 1.696 ± 0.342
2.307AsnArg: 2.307 ± 0.372
2.918AsnSer: 2.918 ± 0.542
3.121AsnThr: 3.121 ± 0.799
2.646AsnVal: 2.646 ± 0.367
0.814AsnTrp: 0.814 ± 0.282
1.493AsnTyr: 1.493 ± 0.351
0.0AsnXaa: 0.0 ± 0.0
Pro
2.511ProAla: 2.511 ± 0.424
0.339ProCys: 0.339 ± 0.157
2.375ProAsp: 2.375 ± 0.478
3.596ProGlu: 3.596 ± 0.491
1.968ProPhe: 1.968 ± 0.344
0.0ProGly: 0.0 ± 0.0
0.746ProHis: 0.746 ± 0.268
2.239ProIle: 2.239 ± 0.505
2.443ProLys: 2.443 ± 0.413
3.529ProLeu: 3.529 ± 0.509
0.95ProMet: 0.95 ± 0.236
1.493ProAsn: 1.493 ± 0.305
1.289ProPro: 1.289 ± 0.367
0.746ProGln: 0.746 ± 0.231
1.154ProArg: 1.154 ± 0.258
2.171ProSer: 2.171 ± 0.511
2.036ProThr: 2.036 ± 0.435
2.036ProVal: 2.036 ± 0.345
0.271ProTrp: 0.271 ± 0.129
0.543ProTyr: 0.543 ± 0.185
0.0ProXaa: 0.0 ± 0.0
Gln
2.375GlnAla: 2.375 ± 0.365
0.271GlnCys: 0.271 ± 0.119
1.9GlnAsp: 1.9 ± 0.397
3.529GlnGlu: 3.529 ± 0.551
1.493GlnPhe: 1.493 ± 0.325
1.764GlnGly: 1.764 ± 0.351
0.611GlnHis: 0.611 ± 0.246
2.375GlnIle: 2.375 ± 0.413
2.511GlnLys: 2.511 ± 0.438
3.257GlnLeu: 3.257 ± 0.473
1.154GlnMet: 1.154 ± 0.273
1.221GlnAsn: 1.221 ± 0.28
1.154GlnPro: 1.154 ± 0.286
0.95GlnGln: 0.95 ± 0.232
1.9GlnArg: 1.9 ± 0.364
1.968GlnSer: 1.968 ± 0.372
1.696GlnThr: 1.696 ± 0.418
2.443GlnVal: 2.443 ± 0.437
0.611GlnTrp: 0.611 ± 0.204
1.561GlnTyr: 1.561 ± 0.415
0.0GlnXaa: 0.0 ± 0.0
Arg
3.393ArgAla: 3.393 ± 0.535
0.271ArgCys: 0.271 ± 0.132
1.9ArgAsp: 1.9 ± 0.352
3.868ArgGlu: 3.868 ± 0.694
2.171ArgPhe: 2.171 ± 0.399
2.646ArgGly: 2.646 ± 0.397
0.543ArgHis: 0.543 ± 0.208
3.257ArgIle: 3.257 ± 0.433
3.8ArgLys: 3.8 ± 0.453
2.85ArgLeu: 2.85 ± 0.507
0.95ArgMet: 0.95 ± 0.263
2.036ArgAsn: 2.036 ± 0.534
1.018ArgPro: 1.018 ± 0.28
1.696ArgGln: 1.696 ± 0.322
2.918ArgArg: 2.918 ± 0.473
1.968ArgSer: 1.968 ± 0.351
2.986ArgThr: 2.986 ± 0.612
3.054ArgVal: 3.054 ± 0.493
0.679ArgTrp: 0.679 ± 0.223
1.832ArgTyr: 1.832 ± 0.422
0.0ArgXaa: 0.0 ± 0.0
Ser
3.596SerAla: 3.596 ± 0.788
0.814SerCys: 0.814 ± 0.247
3.393SerAsp: 3.393 ± 0.531
4.004SerGlu: 4.004 ± 0.512
2.85SerPhe: 2.85 ± 0.326
5.021SerGly: 5.021 ± 0.72
1.493SerHis: 1.493 ± 0.367
5.429SerIle: 5.429 ± 0.545
5.157SerLys: 5.157 ± 0.693
5.089SerLeu: 5.089 ± 0.539
1.764SerMet: 1.764 ± 0.252
3.596SerAsn: 3.596 ± 0.534
1.425SerPro: 1.425 ± 0.394
2.714SerGln: 2.714 ± 0.371
1.9SerArg: 1.9 ± 0.313
4.546SerSer: 4.546 ± 0.64
3.596SerThr: 3.596 ± 0.782
3.393SerVal: 3.393 ± 0.575
0.814SerTrp: 0.814 ± 0.24
1.968SerTyr: 1.968 ± 0.41
0.0SerXaa: 0.0 ± 0.0
Thr
4.479ThrAla: 4.479 ± 0.691
0.475ThrCys: 0.475 ± 0.201
3.393ThrAsp: 3.393 ± 0.462
4.886ThrGlu: 4.886 ± 0.499
3.121ThrPhe: 3.121 ± 0.518
5.089ThrGly: 5.089 ± 0.65
1.086ThrHis: 1.086 ± 0.258
3.8ThrIle: 3.8 ± 0.479
3.732ThrLys: 3.732 ± 0.562
4.139ThrLeu: 4.139 ± 0.622
1.154ThrMet: 1.154 ± 0.245
3.054ThrAsn: 3.054 ± 0.708
3.054ThrPro: 3.054 ± 0.582
1.696ThrGln: 1.696 ± 0.35
2.375ThrArg: 2.375 ± 0.516
4.071ThrSer: 4.071 ± 0.978
3.868ThrThr: 3.868 ± 0.618
4.343ThrVal: 4.343 ± 0.549
1.018ThrTrp: 1.018 ± 0.404
2.714ThrTyr: 2.714 ± 0.486
0.0ThrXaa: 0.0 ± 0.0
Val
4.614ValAla: 4.614 ± 0.778
0.746ValCys: 0.746 ± 0.22
4.004ValAsp: 4.004 ± 0.512
3.868ValGlu: 3.868 ± 0.557
2.714ValPhe: 2.714 ± 0.365
3.664ValGly: 3.664 ± 0.546
0.95ValHis: 0.95 ± 0.217
5.429ValIle: 5.429 ± 0.601
4.546ValLys: 4.546 ± 0.631
4.818ValLeu: 4.818 ± 0.66
1.357ValMet: 1.357 ± 0.262
3.461ValAsn: 3.461 ± 0.441
1.493ValPro: 1.493 ± 0.3
2.443ValGln: 2.443 ± 0.428
2.782ValArg: 2.782 ± 0.418
3.868ValSer: 3.868 ± 0.644
4.139ValThr: 4.139 ± 0.533
3.393ValVal: 3.393 ± 0.616
0.339ValTrp: 0.339 ± 0.176
2.375ValTyr: 2.375 ± 0.348
0.0ValXaa: 0.0 ± 0.0
Trp
0.475TrpAla: 0.475 ± 0.16
0.068TrpCys: 0.068 ± 0.059
0.611TrpAsp: 0.611 ± 0.214
0.611TrpGlu: 0.611 ± 0.208
0.407TrpPhe: 0.407 ± 0.184
0.814TrpGly: 0.814 ± 0.241
0.136TrpHis: 0.136 ± 0.085
0.95TrpIle: 0.95 ± 0.248
0.611TrpLys: 0.611 ± 0.194
0.95TrpLeu: 0.95 ± 0.243
0.475TrpMet: 0.475 ± 0.195
0.95TrpAsn: 0.95 ± 0.238
0.0TrpPro: 0.0 ± 0.0
0.271TrpGln: 0.271 ± 0.117
0.611TrpArg: 0.611 ± 0.216
1.221TrpSer: 1.221 ± 0.376
0.679TrpThr: 0.679 ± 0.245
0.611TrpVal: 0.611 ± 0.286
0.271TrpTrp: 0.271 ± 0.147
0.543TrpTyr: 0.543 ± 0.183
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.9TyrAla: 1.9 ± 0.337
0.204TyrCys: 0.204 ± 0.116
2.986TyrAsp: 2.986 ± 0.503
3.732TyrGlu: 3.732 ± 0.446
1.289TyrPhe: 1.289 ± 0.299
3.868TyrGly: 3.868 ± 0.543
0.882TyrHis: 0.882 ± 0.224
2.782TyrIle: 2.782 ± 0.464
3.054TyrLys: 3.054 ± 0.47
3.461TyrLeu: 3.461 ± 0.567
1.086TyrMet: 1.086 ± 0.289
1.493TyrAsn: 1.493 ± 0.309
0.611TyrPro: 0.611 ± 0.235
0.882TyrGln: 0.882 ± 0.236
1.832TyrArg: 1.832 ± 0.432
2.443TyrSer: 2.443 ± 0.361
3.596TyrThr: 3.596 ± 0.526
1.9TyrVal: 1.9 ± 0.419
0.271TyrTrp: 0.271 ± 0.128
1.289TyrTyr: 1.289 ± 0.324
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (14738 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski