Paenibacillus sp. CAA11

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2S0UDJ0|A0A2S0UDJ0_9BACL Signal peptidase I OS=Paenibacillus sp. CAA11 OX=1532905 GN=DCC85_17810 PE=4 SV=1
MM1 pKa = 7.57RR2 pKa = 11.84PIIEE6 pKa = 4.29FCTNNMHH13 pKa = 7.32FGTDD17 pKa = 3.01EE18 pKa = 3.97VMEE21 pKa = 4.44KK22 pKa = 10.91LEE24 pKa = 4.47EE25 pKa = 4.28NPDD28 pKa = 3.76YY29 pKa = 11.31DD30 pKa = 3.83VLEE33 pKa = 4.27YY34 pKa = 11.31GCLNNCGQCFMMPYY48 pKa = 10.48ALVNGEE54 pKa = 3.93IVEE57 pKa = 4.44ADD59 pKa = 3.86SADD62 pKa = 3.55QLYY65 pKa = 11.09DD66 pKa = 4.58RR67 pKa = 11.84ILAKK71 pKa = 10.29IKK73 pKa = 10.31EE74 pKa = 4.17LEE76 pKa = 3.97AWDD79 pKa = 5.39ALDD82 pKa = 5.31LDD84 pKa = 4.34

Molecular weight:
9.68 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2S0UFY9|A0A2S0UFY9_9BACL Polysacc_synt_C domain-containing protein OS=Paenibacillus sp. CAA11 OX=1532905 GN=DCC85_00225 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.95KK14 pKa = 8.25VHH16 pKa = 5.49GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight:
5.12 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4316

0

4316

1369789

25

2524

317.4

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.199 ± 0.044

0.759 ± 0.013

5.012 ± 0.028

7.049 ± 0.047

3.948 ± 0.03

7.493 ± 0.035

2.11 ± 0.019

6.582 ± 0.036

5.521 ± 0.036

10.409 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.019

3.623 ± 0.029

4.003 ± 0.026

4.065 ± 0.028

5.157 ± 0.033

6.426 ± 0.034

5.216 ± 0.034

6.956 ± 0.032

1.202 ± 0.017

3.526 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski