Amino acid dipepetide frequency for Paenibacillus sp. CAA11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.111AlaAla: 9.111 ± 0.122
0.727AlaCys: 0.727 ± 0.025
4.182AlaAsp: 4.182 ± 0.065
6.258AlaGlu: 6.258 ± 0.083
3.106AlaPhe: 3.106 ± 0.046
6.891AlaGly: 6.891 ± 0.089
1.399AlaHis: 1.399 ± 0.035
4.934AlaIle: 4.934 ± 0.068
4.635AlaLys: 4.635 ± 0.064
8.377AlaLeu: 8.377 ± 0.084
2.202AlaMet: 2.202 ± 0.042
2.413AlaAsn: 2.413 ± 0.043
2.724AlaPro: 2.724 ± 0.056
2.822AlaGln: 2.822 ± 0.056
3.617AlaArg: 3.617 ± 0.06
5.222AlaSer: 5.222 ± 0.063
3.186AlaThr: 3.186 ± 0.055
6.568AlaVal: 6.568 ± 0.082
0.928AlaTrp: 0.928 ± 0.026
2.688AlaTyr: 2.688 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.523CysAla: 0.523 ± 0.022
0.122CysCys: 0.122 ± 0.01
0.365CysAsp: 0.365 ± 0.017
0.431CysGlu: 0.431 ± 0.018
0.321CysPhe: 0.321 ± 0.017
0.774CysGly: 0.774 ± 0.025
0.159CysHis: 0.159 ± 0.011
0.496CysIle: 0.496 ± 0.021
0.345CysLys: 0.345 ± 0.016
0.777CysLeu: 0.777 ± 0.023
0.197CysMet: 0.197 ± 0.012
0.259CysAsn: 0.259 ± 0.015
0.347CysPro: 0.347 ± 0.02
0.242CysGln: 0.242 ± 0.013
0.454CysArg: 0.454 ± 0.02
0.575CysSer: 0.575 ± 0.021
0.412CysThr: 0.412 ± 0.017
0.435CysVal: 0.435 ± 0.018
0.091CysTrp: 0.091 ± 0.008
0.264CysTyr: 0.264 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
3.663AspAla: 3.663 ± 0.058
0.357AspCys: 0.357 ± 0.016
2.205AspAsp: 2.205 ± 0.048
3.757AspGlu: 3.757 ± 0.056
2.097AspPhe: 2.097 ± 0.04
3.72AspGly: 3.72 ± 0.062
1.21AspHis: 1.21 ± 0.032
3.405AspIle: 3.405 ± 0.056
2.825AspLys: 2.825 ± 0.056
5.096AspLeu: 5.096 ± 0.06
1.318AspMet: 1.318 ± 0.033
1.635AspAsn: 1.635 ± 0.038
2.432AspPro: 2.432 ± 0.044
2.2AspGln: 2.2 ± 0.037
2.743AspArg: 2.743 ± 0.043
2.834AspSer: 2.834 ± 0.046
2.486AspThr: 2.486 ± 0.045
3.2AspVal: 3.2 ± 0.052
0.746AspTrp: 0.746 ± 0.026
2.195AspTyr: 2.195 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
6.506GluAla: 6.506 ± 0.08
0.392GluCys: 0.392 ± 0.019
3.478GluAsp: 3.478 ± 0.055
6.128GluGlu: 6.128 ± 0.094
2.252GluPhe: 2.252 ± 0.041
4.886GluGly: 4.886 ± 0.079
1.717GluHis: 1.717 ± 0.039
4.504GluIle: 4.504 ± 0.066
3.866GluLys: 3.866 ± 0.064
7.701GluLeu: 7.701 ± 0.094
1.992GluMet: 1.992 ± 0.044
2.403GluAsn: 2.403 ± 0.045
2.363GluPro: 2.363 ± 0.044
3.9GluGln: 3.9 ± 0.057
4.124GluArg: 4.124 ± 0.069
3.482GluSer: 3.482 ± 0.052
3.081GluThr: 3.081 ± 0.049
4.807GluVal: 4.807 ± 0.063
0.81GluTrp: 0.81 ± 0.024
2.094GluTyr: 2.094 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.091PheAla: 3.091 ± 0.053
0.347PheCys: 0.347 ± 0.014
2.078PheAsp: 2.078 ± 0.041
2.419PheGlu: 2.419 ± 0.046
1.728PhePhe: 1.728 ± 0.041
3.117PheGly: 3.117 ± 0.055
0.861PheHis: 0.861 ± 0.025
2.762PheIle: 2.762 ± 0.05
1.994PheLys: 1.994 ± 0.042
3.774PheLeu: 3.774 ± 0.063
1.153PheMet: 1.153 ± 0.031
1.554PheAsn: 1.554 ± 0.038
1.443PhePro: 1.443 ± 0.032
1.343PheGln: 1.343 ± 0.035
1.931PheArg: 1.931 ± 0.038
2.754PheSer: 2.754 ± 0.053
2.389PheThr: 2.389 ± 0.044
2.773PheVal: 2.773 ± 0.048
0.502PheTrp: 0.502 ± 0.018
1.464PheTyr: 1.464 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
5.624GlyAla: 5.624 ± 0.074
0.672GlyCys: 0.672 ± 0.02
3.552GlyAsp: 3.552 ± 0.059
4.744GlyGlu: 4.744 ± 0.061
3.163GlyPhe: 3.163 ± 0.057
5.75GlyGly: 5.75 ± 0.091
1.52GlyHis: 1.52 ± 0.036
5.612GlyIle: 5.612 ± 0.063
4.571GlyLys: 4.571 ± 0.068
7.606GlyLeu: 7.606 ± 0.09
2.286GlyMet: 2.286 ± 0.047
2.559GlyAsn: 2.559 ± 0.053
1.962GlyPro: 1.962 ± 0.036
2.952GlyGln: 2.952 ± 0.052
3.85GlyArg: 3.85 ± 0.068
4.996GlySer: 4.996 ± 0.076
4.373GlyThr: 4.373 ± 0.065
5.107GlyVal: 5.107 ± 0.068
1.029GlyTrp: 1.029 ± 0.032
3.006GlyTyr: 3.006 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
1.543HisAla: 1.543 ± 0.037
0.189HisCys: 0.189 ± 0.012
0.977HisAsp: 0.977 ± 0.025
1.376HisGlu: 1.376 ± 0.029
0.95HisPhe: 0.95 ± 0.03
1.586HisGly: 1.586 ± 0.033
0.604HisHis: 0.604 ± 0.023
1.431HisIle: 1.431 ± 0.037
0.917HisLys: 0.917 ± 0.029
2.197HisLeu: 2.197 ± 0.076
0.555HisMet: 0.555 ± 0.018
0.704HisAsn: 0.704 ± 0.024
1.253HisPro: 1.253 ± 0.032
0.867HisGln: 0.867 ± 0.027
1.121HisArg: 1.121 ± 0.026
1.243HisSer: 1.243 ± 0.03
1.069HisThr: 1.069 ± 0.028
1.363HisVal: 1.363 ± 0.032
0.306HisTrp: 0.306 ± 0.013
0.852HisTyr: 0.852 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
5.623IleAla: 5.623 ± 0.068
0.6IleCys: 0.6 ± 0.024
3.348IleAsp: 3.348 ± 0.059
4.23IleGlu: 4.23 ± 0.067
2.479IlePhe: 2.479 ± 0.047
5.183IleGly: 5.183 ± 0.078
1.521IleHis: 1.521 ± 0.036
4.062IleIle: 4.062 ± 0.07
3.116IleLys: 3.116 ± 0.046
6.209IleLeu: 6.209 ± 0.086
1.605IleMet: 1.605 ± 0.037
2.265IleAsn: 2.265 ± 0.041
3.067IlePro: 3.067 ± 0.042
2.633IleGln: 2.633 ± 0.047
3.69IleArg: 3.69 ± 0.055
4.602IleSer: 4.602 ± 0.065
3.893IleThr: 3.893 ± 0.057
4.794IleVal: 4.794 ± 0.062
0.678IleTrp: 0.678 ± 0.023
2.218IleTyr: 2.218 ± 0.04
0.0IleXaa: 0.0 ± 0.0
Lys
4.474LysAla: 4.474 ± 0.06
0.242LysCys: 0.242 ± 0.014
3.049LysAsp: 3.049 ± 0.052
4.509LysGlu: 4.509 ± 0.067
1.567LysPhe: 1.567 ± 0.032
3.736LysGly: 3.736 ± 0.056
1.126LysHis: 1.126 ± 0.028
3.13LysIle: 3.13 ± 0.05
3.363LysLys: 3.363 ± 0.064
5.793LysLeu: 5.793 ± 0.058
1.61LysMet: 1.61 ± 0.033
2.072LysAsn: 2.072 ± 0.044
2.356LysPro: 2.356 ± 0.043
2.711LysGln: 2.711 ± 0.043
2.917LysArg: 2.917 ± 0.048
3.171LysSer: 3.171 ± 0.051
2.752LysThr: 2.752 ± 0.053
3.974LysVal: 3.974 ± 0.063
0.713LysTrp: 0.713 ± 0.019
1.948LysTyr: 1.948 ± 0.042
0.0LysXaa: 0.0 ± 0.0
Leu
8.581LeuAla: 8.581 ± 0.101
0.85LeuCys: 0.85 ± 0.022
5.256LeuAsp: 5.256 ± 0.07
6.757LeuGlu: 6.757 ± 0.086
4.342LeuPhe: 4.342 ± 0.074
7.345LeuGly: 7.345 ± 0.076
2.13LeuHis: 2.13 ± 0.039
6.765LeuIle: 6.765 ± 0.088
5.826LeuLys: 5.826 ± 0.064
11.508LeuLeu: 11.508 ± 0.128
2.744LeuMet: 2.744 ± 0.043
3.909LeuAsn: 3.909 ± 0.058
4.55LeuPro: 4.55 ± 0.067
4.304LeuGln: 4.304 ± 0.065
5.251LeuArg: 5.251 ± 0.071
7.43LeuSer: 7.43 ± 0.088
5.681LeuThr: 5.681 ± 0.066
6.5LeuVal: 6.5 ± 0.076
1.066LeuTrp: 1.066 ± 0.029
3.291LeuTyr: 3.291 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.165MetAla: 2.165 ± 0.041
0.155MetCys: 0.155 ± 0.011
1.529MetAsp: 1.529 ± 0.033
1.862MetGlu: 1.862 ± 0.038
0.944MetPhe: 0.944 ± 0.029
1.779MetGly: 1.779 ± 0.034
0.423MetHis: 0.423 ± 0.017
1.884MetIle: 1.884 ± 0.038
2.017MetLys: 2.017 ± 0.038
2.907MetLeu: 2.907 ± 0.053
0.794MetMet: 0.794 ± 0.026
1.525MetAsn: 1.525 ± 0.032
1.067MetPro: 1.067 ± 0.029
1.002MetGln: 1.002 ± 0.026
1.27MetArg: 1.27 ± 0.029
1.788MetSer: 1.788 ± 0.039
1.588MetThr: 1.588 ± 0.033
1.799MetVal: 1.799 ± 0.036
0.213MetTrp: 0.213 ± 0.014
0.735MetTyr: 0.735 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
2.602AsnAla: 2.602 ± 0.046
0.266AsnCys: 0.266 ± 0.014
1.708AsnAsp: 1.708 ± 0.043
2.39AsnGlu: 2.39 ± 0.042
1.299AsnPhe: 1.299 ± 0.032
2.877AsnGly: 2.877 ± 0.058
0.845AsnHis: 0.845 ± 0.028
2.372AsnIle: 2.372 ± 0.05
2.05AsnLys: 2.05 ± 0.034
3.439AsnLeu: 3.439 ± 0.046
1.004AsnMet: 1.004 ± 0.025
1.481AsnAsn: 1.481 ± 0.036
1.966AsnPro: 1.966 ± 0.042
1.488AsnGln: 1.488 ± 0.032
2.002AsnArg: 2.002 ± 0.04
2.168AsnSer: 2.168 ± 0.043
1.931AsnThr: 1.931 ± 0.044
2.391AsnVal: 2.391 ± 0.044
0.505AsnTrp: 0.505 ± 0.02
1.44AsnTyr: 1.44 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
3.402ProAla: 3.402 ± 0.059
0.235ProCys: 0.235 ± 0.016
2.494ProAsp: 2.494 ± 0.043
3.58ProGlu: 3.58 ± 0.056
1.767ProPhe: 1.767 ± 0.041
3.117ProGly: 3.117 ± 0.058
0.867ProHis: 0.867 ± 0.028
2.321ProIle: 2.321 ± 0.042
1.992ProLys: 1.992 ± 0.041
4.051ProLeu: 4.051 ± 0.06
0.946ProMet: 0.946 ± 0.024
1.448ProAsn: 1.448 ± 0.038
1.221ProPro: 1.221 ± 0.041
1.432ProGln: 1.432 ± 0.032
1.445ProArg: 1.445 ± 0.036
2.659ProSer: 2.659 ± 0.045
1.774ProThr: 1.774 ± 0.041
3.222ProVal: 3.222 ± 0.054
0.497ProTrp: 0.497 ± 0.022
1.562ProTyr: 1.562 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
3.701GlnAla: 3.701 ± 0.048
0.203GlnCys: 0.203 ± 0.013
1.855GlnAsp: 1.855 ± 0.039
3.103GlnGlu: 3.103 ± 0.054
1.483GlnPhe: 1.483 ± 0.036
3.056GlnGly: 3.056 ± 0.05
0.887GlnHis: 0.887 ± 0.036
2.716GlnIle: 2.716 ± 0.043
2.016GlnLys: 2.016 ± 0.037
4.548GlnLeu: 4.548 ± 0.06
1.132GlnMet: 1.132 ± 0.029
1.38GlnAsn: 1.38 ± 0.033
1.575GlnPro: 1.575 ± 0.031
2.097GlnGln: 2.097 ± 0.048
2.092GlnArg: 2.092 ± 0.043
2.327GlnSer: 2.327 ± 0.044
2.049GlnThr: 2.049 ± 0.043
2.73GlnVal: 2.73 ± 0.045
0.458GlnTrp: 0.458 ± 0.019
1.248GlnTyr: 1.248 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
3.379ArgAla: 3.379 ± 0.05
0.371ArgCys: 0.371 ± 0.015
2.383ArgAsp: 2.383 ± 0.048
4.087ArgGlu: 4.087 ± 0.07
2.109ArgPhe: 2.109 ± 0.041
3.108ArgGly: 3.108 ± 0.053
1.064ArgHis: 1.064 ± 0.029
3.737ArgIle: 3.737 ± 0.058
3.191ArgLys: 3.191 ± 0.05
5.577ArgLeu: 5.577 ± 0.07
1.615ArgMet: 1.615 ± 0.032
1.925ArgAsn: 1.925 ± 0.039
1.802ArgPro: 1.802 ± 0.033
2.187ArgGln: 2.187 ± 0.041
3.141ArgArg: 3.141 ± 0.063
3.349ArgSer: 3.349 ± 0.05
2.688ArgThr: 2.688 ± 0.042
3.265ArgVal: 3.265 ± 0.047
0.658ArgTrp: 0.658 ± 0.021
1.932ArgTyr: 1.932 ± 0.041
0.0ArgXaa: 0.0 ± 0.0
Ser
4.995SerAla: 4.995 ± 0.069
0.464SerCys: 0.464 ± 0.017
2.899SerAsp: 2.899 ± 0.046
3.938SerGlu: 3.938 ± 0.057
2.873SerPhe: 2.873 ± 0.057
5.557SerGly: 5.557 ± 0.073
1.234SerHis: 1.234 ± 0.034
4.299SerIle: 4.299 ± 0.062
3.528SerLys: 3.528 ± 0.054
6.724SerLeu: 6.724 ± 0.078
1.781SerMet: 1.781 ± 0.037
2.154SerAsn: 2.154 ± 0.041
2.544SerPro: 2.544 ± 0.048
2.211SerGln: 2.211 ± 0.049
3.427SerArg: 3.427 ± 0.053
4.823SerSer: 4.823 ± 0.076
3.203SerThr: 3.203 ± 0.053
4.443SerVal: 4.443 ± 0.067
0.849SerTrp: 0.849 ± 0.026
2.316SerTyr: 2.316 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
4.658ThrAla: 4.658 ± 0.06
0.364ThrCys: 0.364 ± 0.016
2.739ThrAsp: 2.739 ± 0.051
3.409ThrGlu: 3.409 ± 0.049
2.116ThrPhe: 2.116 ± 0.041
4.578ThrGly: 4.578 ± 0.062
1.015ThrHis: 1.015 ± 0.03
3.288ThrIle: 3.288 ± 0.054
2.431ThrLys: 2.431 ± 0.045
5.506ThrLeu: 5.506 ± 0.067
1.198ThrMet: 1.198 ± 0.027
1.749ThrAsn: 1.749 ± 0.045
2.514ThrPro: 2.514 ± 0.052
1.697ThrGln: 1.697 ± 0.037
2.316ThrArg: 2.316 ± 0.038
3.273ThrSer: 3.273 ± 0.05
2.649ThrThr: 2.649 ± 0.049
4.321ThrVal: 4.321 ± 0.061
0.564ThrTrp: 0.564 ± 0.02
1.778ThrTyr: 1.778 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
4.965ValAla: 4.965 ± 0.068
0.631ValCys: 0.631 ± 0.023
3.517ValAsp: 3.517 ± 0.055
4.335ValGlu: 4.335 ± 0.058
2.774ValPhe: 2.774 ± 0.047
4.422ValGly: 4.422 ± 0.057
1.51ValHis: 1.51 ± 0.034
5.038ValIle: 5.038 ± 0.067
4.033ValLys: 4.033 ± 0.06
7.4ValLeu: 7.4 ± 0.091
1.984ValMet: 1.984 ± 0.044
2.843ValAsn: 2.843 ± 0.047
2.96ValPro: 2.96 ± 0.052
2.741ValGln: 2.741 ± 0.047
3.422ValArg: 3.422 ± 0.044
4.59ValSer: 4.59 ± 0.066
4.344ValThr: 4.344 ± 0.064
4.877ValVal: 4.877 ± 0.067
0.768ValTrp: 0.768 ± 0.022
2.399ValTyr: 2.399 ± 0.041
0.0ValXaa: 0.0 ± 0.0
Trp
0.767TrpAla: 0.767 ± 0.026
0.088TrpCys: 0.088 ± 0.008
0.683TrpAsp: 0.683 ± 0.025
0.755TrpGlu: 0.755 ± 0.024
0.541TrpPhe: 0.541 ± 0.022
0.796TrpGly: 0.796 ± 0.027
0.217TrpHis: 0.217 ± 0.014
0.856TrpIle: 0.856 ± 0.026
0.71TrpLys: 0.71 ± 0.025
1.37TrpLeu: 1.37 ± 0.037
0.43TrpMet: 0.43 ± 0.02
0.619TrpAsn: 0.619 ± 0.023
0.336TrpPro: 0.336 ± 0.013
0.449TrpGln: 0.449 ± 0.016
0.618TrpArg: 0.618 ± 0.024
0.792TrpSer: 0.792 ± 0.025
0.658TrpThr: 0.658 ± 0.024
0.749TrpVal: 0.749 ± 0.024
0.177TrpTrp: 0.177 ± 0.013
0.405TrpTyr: 0.405 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.616TyrAla: 2.616 ± 0.045
0.312TyrCys: 0.312 ± 0.014
1.826TyrAsp: 1.826 ± 0.041
2.418TyrGlu: 2.418 ± 0.046
1.565TyrPhe: 1.565 ± 0.034
2.656TyrGly: 2.656 ± 0.048
0.793TyrHis: 0.793 ± 0.025
2.206TyrIle: 2.206 ± 0.045
1.784TyrLys: 1.784 ± 0.037
3.525TyrLeu: 3.525 ± 0.058
0.902TyrMet: 0.902 ± 0.025
1.332TyrAsn: 1.332 ± 0.038
1.549TyrPro: 1.549 ± 0.032
1.373TyrGln: 1.373 ± 0.032
2.138TyrArg: 2.138 ± 0.038
2.184TyrSer: 2.184 ± 0.039
1.958TyrThr: 1.958 ± 0.047
2.236TyrVal: 2.236 ± 0.039
0.462TyrTrp: 0.462 ± 0.02
1.429TyrTyr: 1.429 ± 0.037
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4316 proteins (1369789 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski