Amino acid dipepetide frequency for Escherichia phage Mt1B1_P10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.737AlaAla: 8.737 ± 1.422
0.296AlaCys: 0.296 ± 0.141
5.627AlaAsp: 5.627 ± 0.699
5.997AlaGlu: 5.997 ± 0.645
3.332AlaPhe: 3.332 ± 0.566
7.182AlaGly: 7.182 ± 1.04
1.259AlaHis: 1.259 ± 0.237
5.183AlaIle: 5.183 ± 0.398
5.035AlaLys: 5.035 ± 0.608
7.7AlaLeu: 7.7 ± 0.83
2.962AlaMet: 2.962 ± 0.498
3.554AlaAsn: 3.554 ± 0.432
3.554AlaPro: 3.554 ± 0.538
5.035AlaGln: 5.035 ± 0.926
5.109AlaArg: 5.109 ± 0.791
4.591AlaSer: 4.591 ± 0.833
4.665AlaThr: 4.665 ± 0.585
5.849AlaVal: 5.849 ± 0.72
1.333AlaTrp: 1.333 ± 0.421
3.036AlaTyr: 3.036 ± 0.515
0.0AlaXaa: 0.0 ± 0.0
Cys
0.814CysAla: 0.814 ± 0.341
0.222CysCys: 0.222 ± 0.171
0.666CysAsp: 0.666 ± 0.265
0.518CysGlu: 0.518 ± 0.241
0.592CysPhe: 0.592 ± 0.206
0.814CysGly: 0.814 ± 0.283
0.444CysHis: 0.444 ± 0.242
0.74CysIle: 0.74 ± 0.256
0.814CysLys: 0.814 ± 0.31
0.963CysLeu: 0.963 ± 0.305
0.444CysMet: 0.444 ± 0.197
0.518CysAsn: 0.518 ± 0.252
0.444CysPro: 0.444 ± 0.209
0.296CysGln: 0.296 ± 0.159
0.963CysArg: 0.963 ± 0.302
0.222CysSer: 0.222 ± 0.126
0.148CysThr: 0.148 ± 0.103
0.518CysVal: 0.518 ± 0.264
0.148CysTrp: 0.148 ± 0.106
0.666CysTyr: 0.666 ± 0.219
0.0CysXaa: 0.0 ± 0.0
Asp
6.071AspAla: 6.071 ± 0.613
0.666AspCys: 0.666 ± 0.224
3.184AspAsp: 3.184 ± 0.665
4.739AspGlu: 4.739 ± 0.665
2.073AspPhe: 2.073 ± 0.439
4.368AspGly: 4.368 ± 0.753
0.74AspHis: 0.74 ± 0.229
4.146AspIle: 4.146 ± 0.533
4.294AspLys: 4.294 ± 0.527
4.739AspLeu: 4.739 ± 0.637
1.999AspMet: 1.999 ± 0.424
3.184AspAsn: 3.184 ± 0.522
2.073AspPro: 2.073 ± 0.327
1.333AspGln: 1.333 ± 0.321
2.888AspArg: 2.888 ± 0.542
4.146AspSer: 4.146 ± 0.628
3.48AspThr: 3.48 ± 0.54
3.85AspVal: 3.85 ± 0.574
1.037AspTrp: 1.037 ± 0.324
2.073AspTyr: 2.073 ± 0.424
0.0AspXaa: 0.0 ± 0.0
Glu
7.182GluAla: 7.182 ± 0.958
0.814GluCys: 0.814 ± 0.31
4.813GluAsp: 4.813 ± 0.55
6.145GluGlu: 6.145 ± 0.801
2.295GluPhe: 2.295 ± 0.4
5.257GluGly: 5.257 ± 0.606
1.407GluHis: 1.407 ± 0.364
2.221GluIle: 2.221 ± 0.351
4.368GluLys: 4.368 ± 0.509
5.627GluLeu: 5.627 ± 0.754
1.703GluMet: 1.703 ± 0.312
1.999GluAsn: 1.999 ± 0.435
2.073GluPro: 2.073 ± 0.396
3.184GluGln: 3.184 ± 0.556
3.48GluArg: 3.48 ± 0.473
3.628GluSer: 3.628 ± 0.48
2.888GluThr: 2.888 ± 0.42
6.071GluVal: 6.071 ± 0.654
1.629GluTrp: 1.629 ± 0.293
2.073GluTyr: 2.073 ± 0.362
0.0GluXaa: 0.0 ± 0.0
Phe
2.665PheAla: 2.665 ± 0.411
0.148PheCys: 0.148 ± 0.114
3.184PheAsp: 3.184 ± 0.504
2.591PheGlu: 2.591 ± 0.369
1.333PhePhe: 1.333 ± 0.377
2.888PheGly: 2.888 ± 0.535
0.592PheHis: 0.592 ± 0.216
2.369PheIle: 2.369 ± 0.334
3.036PheLys: 3.036 ± 0.494
2.74PheLeu: 2.74 ± 0.443
1.703PheMet: 1.703 ± 0.298
2.073PheAsn: 2.073 ± 0.359
1.111PhePro: 1.111 ± 0.308
1.481PheGln: 1.481 ± 0.301
1.703PheArg: 1.703 ± 0.35
2.221PheSer: 2.221 ± 0.38
2.073PheThr: 2.073 ± 0.283
1.555PheVal: 1.555 ± 0.491
0.222PheTrp: 0.222 ± 0.141
0.963PheTyr: 0.963 ± 0.323
0.0PheXaa: 0.0 ± 0.0
Gly
6.664GlyAla: 6.664 ± 0.686
0.888GlyCys: 0.888 ± 0.351
6.368GlyAsp: 6.368 ± 0.762
5.331GlyGlu: 5.331 ± 0.701
2.962GlyPhe: 2.962 ± 0.574
5.701GlyGly: 5.701 ± 0.837
2.221GlyHis: 2.221 ± 0.42
4.813GlyIle: 4.813 ± 0.654
6.442GlyLys: 6.442 ± 0.878
5.701GlyLeu: 5.701 ± 0.903
2.073GlyMet: 2.073 ± 0.365
2.888GlyAsn: 2.888 ± 0.413
0.37GlyPro: 0.37 ± 0.135
3.11GlyGln: 3.11 ± 0.501
3.628GlyArg: 3.628 ± 0.43
4.961GlySer: 4.961 ± 0.794
4.294GlyThr: 4.294 ± 0.705
5.331GlyVal: 5.331 ± 0.777
1.629GlyTrp: 1.629 ± 0.368
2.962GlyTyr: 2.962 ± 0.495
0.0GlyXaa: 0.0 ± 0.0
His
1.185HisAla: 1.185 ± 0.342
0.37HisCys: 0.37 ± 0.172
1.481HisAsp: 1.481 ± 0.317
1.037HisGlu: 1.037 ± 0.291
0.963HisPhe: 0.963 ± 0.195
1.925HisGly: 1.925 ± 0.48
0.518HisHis: 0.518 ± 0.196
0.963HisIle: 0.963 ± 0.232
1.555HisLys: 1.555 ± 0.441
2.369HisLeu: 2.369 ± 0.493
0.518HisMet: 0.518 ± 0.26
1.037HisAsn: 1.037 ± 0.275
0.888HisPro: 0.888 ± 0.291
0.74HisGln: 0.74 ± 0.233
1.481HisArg: 1.481 ± 0.427
1.037HisSer: 1.037 ± 0.31
0.888HisThr: 0.888 ± 0.226
1.703HisVal: 1.703 ± 0.477
0.148HisTrp: 0.148 ± 0.103
0.888HisTyr: 0.888 ± 0.24
0.0HisXaa: 0.0 ± 0.0
Ile
4.591IleAla: 4.591 ± 0.676
0.296IleCys: 0.296 ± 0.139
3.702IleAsp: 3.702 ± 0.429
3.628IleGlu: 3.628 ± 0.489
1.851IlePhe: 1.851 ± 0.448
4.294IleGly: 4.294 ± 0.535
1.111IleHis: 1.111 ± 0.29
3.332IleIle: 3.332 ± 0.642
3.776IleLys: 3.776 ± 0.623
3.406IleLeu: 3.406 ± 0.572
1.481IleMet: 1.481 ± 0.324
1.925IleAsn: 1.925 ± 0.388
2.369IlePro: 2.369 ± 0.451
2.073IleGln: 2.073 ± 0.418
3.184IleArg: 3.184 ± 0.484
2.295IleSer: 2.295 ± 0.36
3.332IleThr: 3.332 ± 0.634
3.48IleVal: 3.48 ± 0.612
0.963IleTrp: 0.963 ± 0.244
1.629IleTyr: 1.629 ± 0.469
0.0IleXaa: 0.0 ± 0.0
Lys
7.404LysAla: 7.404 ± 0.715
0.666LysCys: 0.666 ± 0.219
3.406LysAsp: 3.406 ± 0.614
4.665LysGlu: 4.665 ± 0.61
1.703LysPhe: 1.703 ± 0.388
6.219LysGly: 6.219 ± 0.755
2.147LysHis: 2.147 ± 0.439
3.258LysIle: 3.258 ± 0.411
4.591LysLys: 4.591 ± 0.674
4.887LysLeu: 4.887 ± 0.679
1.555LysMet: 1.555 ± 0.358
2.073LysAsn: 2.073 ± 0.437
3.036LysPro: 3.036 ± 0.555
2.443LysGln: 2.443 ± 0.407
3.628LysArg: 3.628 ± 0.489
3.776LysSer: 3.776 ± 0.536
2.888LysThr: 2.888 ± 0.381
3.924LysVal: 3.924 ± 0.773
0.963LysTrp: 0.963 ± 0.298
2.517LysTyr: 2.517 ± 0.333
0.0LysXaa: 0.0 ± 0.0
Leu
6.219LeuAla: 6.219 ± 0.739
0.888LeuCys: 0.888 ± 0.304
5.553LeuAsp: 5.553 ± 0.608
5.035LeuGlu: 5.035 ± 0.71
2.665LeuPhe: 2.665 ± 0.472
6.59LeuGly: 6.59 ± 0.986
1.777LeuHis: 1.777 ± 0.513
3.628LeuIle: 3.628 ± 0.46
5.257LeuLys: 5.257 ± 0.531
5.923LeuLeu: 5.923 ± 0.925
2.369LeuMet: 2.369 ± 0.667
3.11LeuAsn: 3.11 ± 0.403
3.332LeuPro: 3.332 ± 0.486
3.554LeuGln: 3.554 ± 0.685
4.146LeuArg: 4.146 ± 0.768
4.591LeuSer: 4.591 ± 0.709
5.109LeuThr: 5.109 ± 0.559
5.775LeuVal: 5.775 ± 0.642
0.888LeuTrp: 0.888 ± 0.284
2.221LeuTyr: 2.221 ± 0.346
0.0LeuXaa: 0.0 ± 0.0
Met
3.036MetAla: 3.036 ± 0.327
0.148MetCys: 0.148 ± 0.113
1.481MetAsp: 1.481 ± 0.326
1.777MetGlu: 1.777 ± 0.269
0.814MetPhe: 0.814 ± 0.292
1.925MetGly: 1.925 ± 0.418
0.888MetHis: 0.888 ± 0.284
1.111MetIle: 1.111 ± 0.361
1.407MetLys: 1.407 ± 0.293
2.74MetLeu: 2.74 ± 0.569
0.814MetMet: 0.814 ± 0.298
1.185MetAsn: 1.185 ± 0.277
1.407MetPro: 1.407 ± 0.307
1.629MetGln: 1.629 ± 0.44
2.147MetArg: 2.147 ± 0.48
2.369MetSer: 2.369 ± 0.395
2.073MetThr: 2.073 ± 0.379
1.925MetVal: 1.925 ± 0.332
0.222MetTrp: 0.222 ± 0.105
0.963MetTyr: 0.963 ± 0.249
0.0MetXaa: 0.0 ± 0.0
Asn
3.924AsnAla: 3.924 ± 0.571
0.666AsnCys: 0.666 ± 0.403
1.999AsnAsp: 1.999 ± 0.36
2.517AsnGlu: 2.517 ± 0.435
1.555AsnPhe: 1.555 ± 0.288
3.776AsnGly: 3.776 ± 0.498
0.814AsnHis: 0.814 ± 0.326
1.851AsnIle: 1.851 ± 0.342
3.11AsnLys: 3.11 ± 0.36
3.48AsnLeu: 3.48 ± 0.464
0.963AsnMet: 0.963 ± 0.401
1.777AsnAsn: 1.777 ± 0.307
2.073AsnPro: 2.073 ± 0.429
1.703AsnGln: 1.703 ± 0.441
2.962AsnArg: 2.962 ± 0.492
2.295AsnSer: 2.295 ± 0.442
2.443AsnThr: 2.443 ± 0.439
3.036AsnVal: 3.036 ± 0.598
0.592AsnTrp: 0.592 ± 0.23
1.777AsnTyr: 1.777 ± 0.301
0.0AsnXaa: 0.0 ± 0.0
Pro
3.11ProAla: 3.11 ± 0.482
0.444ProCys: 0.444 ± 0.175
2.814ProAsp: 2.814 ± 0.658
3.036ProGlu: 3.036 ± 0.548
1.333ProPhe: 1.333 ± 0.256
0.888ProGly: 0.888 ± 0.304
0.74ProHis: 0.74 ± 0.309
0.963ProIle: 0.963 ± 0.289
1.925ProLys: 1.925 ± 0.326
2.888ProLeu: 2.888 ± 0.357
1.185ProMet: 1.185 ± 0.221
1.555ProAsn: 1.555 ± 0.469
0.814ProPro: 0.814 ± 0.209
1.925ProGln: 1.925 ± 0.408
1.185ProArg: 1.185 ± 0.289
2.74ProSer: 2.74 ± 0.413
2.295ProThr: 2.295 ± 0.4
3.332ProVal: 3.332 ± 0.537
0.888ProTrp: 0.888 ± 0.233
1.999ProTyr: 1.999 ± 0.37
0.0ProXaa: 0.0 ± 0.0
Gln
4.517GlnAla: 4.517 ± 0.562
0.518GlnCys: 0.518 ± 0.223
2.295GlnAsp: 2.295 ± 0.383
2.74GlnGlu: 2.74 ± 0.577
1.925GlnPhe: 1.925 ± 0.312
3.554GlnGly: 3.554 ± 0.493
0.74GlnHis: 0.74 ± 0.235
2.443GlnIle: 2.443 ± 0.504
1.703GlnLys: 1.703 ± 0.317
2.888GlnLeu: 2.888 ± 0.548
1.925GlnMet: 1.925 ± 0.364
1.777GlnAsn: 1.777 ± 0.441
1.259GlnPro: 1.259 ± 0.297
2.517GlnGln: 2.517 ± 0.391
2.591GlnArg: 2.591 ± 0.484
1.777GlnSer: 1.777 ± 0.332
1.851GlnThr: 1.851 ± 0.404
2.74GlnVal: 2.74 ± 0.452
0.74GlnTrp: 0.74 ± 0.211
1.555GlnTyr: 1.555 ± 0.434
0.0GlnXaa: 0.0 ± 0.0
Arg
5.109ArgAla: 5.109 ± 0.784
0.592ArgCys: 0.592 ± 0.26
2.665ArgAsp: 2.665 ± 0.404
4.072ArgGlu: 4.072 ± 0.432
1.999ArgPhe: 1.999 ± 0.378
4.739ArgGly: 4.739 ± 0.562
1.259ArgHis: 1.259 ± 0.368
3.332ArgIle: 3.332 ± 0.482
3.11ArgLys: 3.11 ± 0.505
4.665ArgLeu: 4.665 ± 0.635
2.073ArgMet: 2.073 ± 0.344
2.665ArgAsn: 2.665 ± 0.396
1.185ArgPro: 1.185 ± 0.315
2.147ArgGln: 2.147 ± 0.432
3.48ArgArg: 3.48 ± 0.508
2.147ArgSer: 2.147 ± 0.519
2.517ArgThr: 2.517 ± 0.567
3.406ArgVal: 3.406 ± 0.381
0.74ArgTrp: 0.74 ± 0.227
2.443ArgTyr: 2.443 ± 0.445
0.0ArgXaa: 0.0 ± 0.0
Ser
4.739SerAla: 4.739 ± 0.713
0.74SerCys: 0.74 ± 0.272
2.962SerAsp: 2.962 ± 0.435
4.072SerGlu: 4.072 ± 0.462
2.147SerPhe: 2.147 ± 0.428
5.109SerGly: 5.109 ± 0.541
1.111SerHis: 1.111 ± 0.337
2.962SerIle: 2.962 ± 0.553
3.332SerLys: 3.332 ± 0.579
4.591SerLeu: 4.591 ± 0.547
2.295SerMet: 2.295 ± 0.391
2.74SerAsn: 2.74 ± 0.586
1.777SerPro: 1.777 ± 0.34
2.221SerGln: 2.221 ± 0.467
3.258SerArg: 3.258 ± 0.467
3.11SerSer: 3.11 ± 0.637
2.665SerThr: 2.665 ± 0.425
4.072SerVal: 4.072 ± 0.538
0.74SerTrp: 0.74 ± 0.247
2.295SerTyr: 2.295 ± 0.689
0.0SerXaa: 0.0 ± 0.0
Thr
3.924ThrAla: 3.924 ± 0.654
0.74ThrCys: 0.74 ± 0.299
3.184ThrAsp: 3.184 ± 0.721
3.776ThrGlu: 3.776 ± 0.481
2.665ThrPhe: 2.665 ± 0.477
3.85ThrGly: 3.85 ± 0.433
1.333ThrHis: 1.333 ± 0.373
2.888ThrIle: 2.888 ± 0.364
3.998ThrLys: 3.998 ± 0.423
4.591ThrLeu: 4.591 ± 0.632
1.037ThrMet: 1.037 ± 0.245
2.665ThrAsn: 2.665 ± 0.566
2.443ThrPro: 2.443 ± 0.409
2.073ThrGln: 2.073 ± 0.496
2.517ThrArg: 2.517 ± 0.637
3.11ThrSer: 3.11 ± 0.578
3.48ThrThr: 3.48 ± 0.753
3.332ThrVal: 3.332 ± 0.578
0.666ThrTrp: 0.666 ± 0.196
1.703ThrTyr: 1.703 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
6.219ValAla: 6.219 ± 0.753
0.888ValCys: 0.888 ± 0.27
2.814ValAsp: 2.814 ± 0.516
3.998ValGlu: 3.998 ± 0.584
2.221ValPhe: 2.221 ± 0.363
5.257ValGly: 5.257 ± 0.789
1.407ValHis: 1.407 ± 0.324
3.776ValIle: 3.776 ± 0.48
4.887ValLys: 4.887 ± 0.572
4.517ValLeu: 4.517 ± 0.779
1.777ValMet: 1.777 ± 0.355
4.146ValAsn: 4.146 ± 0.679
3.184ValPro: 3.184 ± 0.413
2.74ValGln: 2.74 ± 0.508
3.332ValArg: 3.332 ± 0.505
4.665ValSer: 4.665 ± 0.545
4.517ValThr: 4.517 ± 0.596
4.294ValVal: 4.294 ± 0.682
0.666ValTrp: 0.666 ± 0.199
2.517ValTyr: 2.517 ± 0.417
0.0ValXaa: 0.0 ± 0.0
Trp
0.963TrpAla: 0.963 ± 0.271
0.296TrpCys: 0.296 ± 0.139
0.74TrpAsp: 0.74 ± 0.215
1.407TrpGlu: 1.407 ± 0.247
0.518TrpPhe: 0.518 ± 0.258
0.888TrpGly: 0.888 ± 0.263
0.296TrpHis: 0.296 ± 0.158
0.518TrpIle: 0.518 ± 0.171
1.333TrpLys: 1.333 ± 0.321
0.888TrpLeu: 0.888 ± 0.269
0.222TrpMet: 0.222 ± 0.119
0.37TrpAsn: 0.37 ± 0.187
1.259TrpPro: 1.259 ± 0.266
0.592TrpGln: 0.592 ± 0.216
0.74TrpArg: 0.74 ± 0.288
1.037TrpSer: 1.037 ± 0.289
0.666TrpThr: 0.666 ± 0.213
1.259TrpVal: 1.259 ± 0.295
0.074TrpTrp: 0.074 ± 0.086
0.592TrpTyr: 0.592 ± 0.198
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.962TyrAla: 2.962 ± 0.484
0.666TyrCys: 0.666 ± 0.247
2.147TyrAsp: 2.147 ± 0.45
1.777TyrGlu: 1.777 ± 0.278
1.703TyrPhe: 1.703 ± 0.435
3.11TyrGly: 3.11 ± 0.605
0.888TyrHis: 0.888 ± 0.298
2.221TyrIle: 2.221 ± 0.371
1.777TyrLys: 1.777 ± 0.324
3.332TyrLeu: 3.332 ± 0.609
0.888TyrMet: 0.888 ± 0.242
2.073TyrAsn: 2.073 ± 0.391
1.407TyrPro: 1.407 ± 0.349
1.259TyrGln: 1.259 ± 0.207
1.925TyrArg: 1.925 ± 0.432
2.295TyrSer: 2.295 ± 0.441
1.703TyrThr: 1.703 ± 0.349
2.369TyrVal: 2.369 ± 0.389
0.37TyrTrp: 0.37 ± 0.258
1.185TyrTyr: 1.185 ± 0.401
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (13507 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski