Amino acid dipepetide frequency for Peptoniphilus sp. ING2-D1G

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.202AlaAla: 4.202 ± 0.133
0.605AlaCys: 0.605 ± 0.035
3.298AlaAsp: 3.298 ± 0.093
4.129AlaGlu: 4.129 ± 0.1
2.537AlaPhe: 2.537 ± 0.074
4.07AlaGly: 4.07 ± 0.108
0.898AlaHis: 0.898 ± 0.045
5.695AlaIle: 5.695 ± 0.135
5.17AlaLys: 5.17 ± 0.12
6.102AlaLeu: 6.102 ± 0.123
1.826AlaMet: 1.826 ± 0.065
2.667AlaAsn: 2.667 ± 0.087
1.584AlaPro: 1.584 ± 0.065
1.828AlaGln: 1.828 ± 0.062
2.1AlaArg: 2.1 ± 0.075
3.313AlaSer: 3.313 ± 0.084
3.121AlaThr: 3.121 ± 0.088
4.415AlaVal: 4.415 ± 0.106
0.302AlaTrp: 0.302 ± 0.025
2.134AlaTyr: 2.134 ± 0.064
0.0AlaXaa: 0.0 ± 0.0
Cys
0.538CysAla: 0.538 ± 0.035
0.108CysCys: 0.108 ± 0.019
0.582CysAsp: 0.582 ± 0.038
0.599CysGlu: 0.599 ± 0.042
0.314CysPhe: 0.314 ± 0.026
0.875CysGly: 0.875 ± 0.046
0.137CysHis: 0.137 ± 0.016
0.736CysIle: 0.736 ± 0.04
0.576CysLys: 0.576 ± 0.038
0.637CysLeu: 0.637 ± 0.039
0.183CysMet: 0.183 ± 0.022
0.407CysAsn: 0.407 ± 0.031
0.432CysPro: 0.432 ± 0.039
0.169CysGln: 0.169 ± 0.018
0.264CysArg: 0.264 ± 0.024
0.628CysSer: 0.628 ± 0.039
0.409CysThr: 0.409 ± 0.03
0.548CysVal: 0.548 ± 0.037
0.042CysTrp: 0.042 ± 0.01
0.342CysTyr: 0.342 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
3.074AspAla: 3.074 ± 0.095
0.496AspCys: 0.496 ± 0.035
3.218AspAsp: 3.218 ± 0.106
5.7AspGlu: 5.7 ± 0.107
3.517AspPhe: 3.517 ± 0.087
3.534AspGly: 3.534 ± 0.104
0.713AspHis: 0.713 ± 0.041
6.208AspIle: 6.208 ± 0.13
5.379AspLys: 5.379 ± 0.127
6.02AspLeu: 6.02 ± 0.114
1.554AspMet: 1.554 ± 0.062
3.159AspAsn: 3.159 ± 0.092
1.592AspPro: 1.592 ± 0.064
0.987AspGln: 0.987 ± 0.047
2.214AspArg: 2.214 ± 0.074
3.214AspSer: 3.214 ± 0.068
2.583AspThr: 2.583 ± 0.075
4.021AspVal: 4.021 ± 0.088
0.337AspTrp: 0.337 ± 0.025
3.125AspTyr: 3.125 ± 0.089
0.0AspXaa: 0.0 ± 0.0
Glu
4.137GluAla: 4.137 ± 0.107
0.51GluCys: 0.51 ± 0.036
5.299GluAsp: 5.299 ± 0.107
8.122GluGlu: 8.122 ± 0.155
3.211GluPhe: 3.211 ± 0.075
4.439GluGly: 4.439 ± 0.108
0.888GluHis: 0.888 ± 0.048
8.35GluIle: 8.35 ± 0.143
8.135GluLys: 8.135 ± 0.155
6.931GluLeu: 6.931 ± 0.122
2.096GluMet: 2.096 ± 0.082
5.883GluAsn: 5.883 ± 0.134
1.687GluPro: 1.687 ± 0.111
1.818GluGln: 1.818 ± 0.062
2.669GluArg: 2.669 ± 0.095
3.673GluSer: 3.673 ± 0.083
3.03GluThr: 3.03 ± 0.079
4.949GluVal: 4.949 ± 0.104
0.323GluTrp: 0.323 ± 0.025
3.285GluTyr: 3.285 ± 0.09
0.0GluXaa: 0.0 ± 0.0
Phe
2.678PheAla: 2.678 ± 0.083
0.411PheCys: 0.411 ± 0.028
2.944PheAsp: 2.944 ± 0.092
3.41PheGlu: 3.41 ± 0.083
2.174PhePhe: 2.174 ± 0.082
3.091PheGly: 3.091 ± 0.098
0.555PheHis: 0.555 ± 0.034
4.396PheIle: 4.396 ± 0.131
3.755PheLys: 3.755 ± 0.097
4.24PheLeu: 4.24 ± 0.107
1.158PheMet: 1.158 ± 0.05
2.608PheAsn: 2.608 ± 0.075
1.212PhePro: 1.212 ± 0.047
0.89PheGln: 0.89 ± 0.037
1.459PheArg: 1.459 ± 0.05
3.129PheSer: 3.129 ± 0.096
2.366PheThr: 2.366 ± 0.076
2.847PheVal: 2.847 ± 0.085
0.219PheTrp: 0.219 ± 0.024
1.875PheTyr: 1.875 ± 0.064
0.0PheXaa: 0.0 ± 0.0
Gly
4.392GlyAla: 4.392 ± 0.109
0.626GlyCys: 0.626 ± 0.041
3.346GlyAsp: 3.346 ± 0.088
4.567GlyGlu: 4.567 ± 0.119
3.247GlyPhe: 3.247 ± 0.088
4.605GlyGly: 4.605 ± 0.135
1.056GlyHis: 1.056 ± 0.049
6.387GlyIle: 6.387 ± 0.155
5.354GlyLys: 5.354 ± 0.11
5.48GlyLeu: 5.48 ± 0.121
1.837GlyMet: 1.837 ± 0.072
2.946GlyAsn: 2.946 ± 0.09
1.339GlyPro: 1.339 ± 0.055
1.516GlyGln: 1.516 ± 0.057
2.473GlyArg: 2.473 ± 0.074
3.855GlySer: 3.855 ± 0.093
3.477GlyThr: 3.477 ± 0.088
4.55GlyVal: 4.55 ± 0.112
0.441GlyTrp: 0.441 ± 0.034
3.159GlyTyr: 3.159 ± 0.094
0.0GlyXaa: 0.0 ± 0.0
His
0.708HisAla: 0.708 ± 0.042
0.154HisCys: 0.154 ± 0.015
0.643HisAsp: 0.643 ± 0.035
0.888HisGlu: 0.888 ± 0.046
0.624HisPhe: 0.624 ± 0.036
0.964HisGly: 0.964 ± 0.042
0.234HisHis: 0.234 ± 0.024
1.206HisIle: 1.206 ± 0.046
0.928HisLys: 0.928 ± 0.04
1.181HisLeu: 1.181 ± 0.05
0.337HisMet: 0.337 ± 0.026
0.746HisAsn: 0.746 ± 0.04
0.595HisPro: 0.595 ± 0.034
0.377HisGln: 0.377 ± 0.029
0.618HisArg: 0.618 ± 0.036
0.789HisSer: 0.789 ± 0.047
0.643HisThr: 0.643 ± 0.043
0.776HisVal: 0.776 ± 0.044
0.124HisTrp: 0.124 ± 0.016
0.55HisTyr: 0.55 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
6.328IleAla: 6.328 ± 0.133
0.909IleCys: 0.909 ± 0.046
6.206IleAsp: 6.206 ± 0.118
7.549IleGlu: 7.549 ± 0.142
4.415IlePhe: 4.415 ± 0.121
5.797IleGly: 5.797 ± 0.137
1.164IleHis: 1.164 ± 0.048
8.453IleIle: 8.453 ± 0.155
8.196IleLys: 8.196 ± 0.139
9.008IleLeu: 9.008 ± 0.185
2.166IleMet: 2.166 ± 0.071
5.358IleAsn: 5.358 ± 0.122
3.14IlePro: 3.14 ± 0.089
1.78IleGln: 1.78 ± 0.062
3.298IleArg: 3.298 ± 0.086
6.918IleSer: 6.918 ± 0.146
4.474IleThr: 4.474 ± 0.087
6.026IleVal: 6.026 ± 0.113
0.405IleTrp: 0.405 ± 0.03
3.692IleTyr: 3.692 ± 0.087
0.0IleXaa: 0.0 ± 0.0
Lys
4.751LysAla: 4.751 ± 0.105
0.536LysCys: 0.536 ± 0.037
6.159LysAsp: 6.159 ± 0.136
7.728LysGlu: 7.728 ± 0.137
3.494LysPhe: 3.494 ± 0.085
4.869LysGly: 4.869 ± 0.106
1.012LysHis: 1.012 ± 0.044
8.858LysIle: 8.858 ± 0.155
8.013LysLys: 8.013 ± 0.142
7.298LysLeu: 7.298 ± 0.118
2.357LysMet: 2.357 ± 0.077
6.697LysAsn: 6.697 ± 0.141
2.018LysPro: 2.018 ± 0.08
1.843LysGln: 1.843 ± 0.064
3.167LysArg: 3.167 ± 0.081
5.539LysSer: 5.539 ± 0.116
4.101LysThr: 4.101 ± 0.099
4.97LysVal: 4.97 ± 0.108
0.453LysTrp: 0.453 ± 0.029
4.173LysTyr: 4.173 ± 0.093
0.0LysXaa: 0.0 ± 0.0
Leu
5.134LeuAla: 5.134 ± 0.118
0.761LeuCys: 0.761 ± 0.041
5.253LeuAsp: 5.253 ± 0.123
6.501LeuGlu: 6.501 ± 0.111
3.869LeuPhe: 3.869 ± 0.128
5.942LeuGly: 5.942 ± 0.122
1.023LeuHis: 1.023 ± 0.041
8.167LeuIle: 8.167 ± 0.169
9.196LeuLys: 9.196 ± 0.158
7.823LeuLeu: 7.823 ± 0.164
2.488LeuMet: 2.488 ± 0.07
5.858LeuAsn: 5.858 ± 0.116
2.745LeuPro: 2.745 ± 0.081
1.986LeuGln: 1.986 ± 0.07
3.429LeuArg: 3.429 ± 0.089
7.121LeuSer: 7.121 ± 0.133
4.418LeuThr: 4.418 ± 0.096
5.16LeuVal: 5.16 ± 0.103
0.47LeuTrp: 0.47 ± 0.036
3.391LeuTyr: 3.391 ± 0.088
0.0LeuXaa: 0.0 ± 0.0
Met
1.811MetAla: 1.811 ± 0.063
0.198MetCys: 0.198 ± 0.019
1.742MetAsp: 1.742 ± 0.064
1.997MetGlu: 1.997 ± 0.06
0.869MetPhe: 0.869 ± 0.045
1.984MetGly: 1.984 ± 0.061
0.333MetHis: 0.333 ± 0.022
2.189MetIle: 2.189 ± 0.071
2.516MetLys: 2.516 ± 0.069
2.216MetLeu: 2.216 ± 0.069
0.656MetMet: 0.656 ± 0.039
1.594MetAsn: 1.594 ± 0.062
0.848MetPro: 0.848 ± 0.045
0.713MetGln: 0.713 ± 0.035
0.932MetArg: 0.932 ± 0.044
1.723MetSer: 1.723 ± 0.064
1.295MetThr: 1.295 ± 0.051
1.592MetVal: 1.592 ± 0.059
0.114MetTrp: 0.114 ± 0.015
0.892MetTyr: 0.892 ± 0.044
0.0MetXaa: 0.0 ± 0.0
Asn
2.931AsnAla: 2.931 ± 0.087
0.521AsnCys: 0.521 ± 0.034
2.568AsnAsp: 2.568 ± 0.082
3.749AsnGlu: 3.749 ± 0.098
2.946AsnPhe: 2.946 ± 0.1
2.714AsnGly: 2.714 ± 0.081
0.768AsnHis: 0.768 ± 0.038
6.404AsnIle: 6.404 ± 0.128
4.974AsnLys: 4.974 ± 0.121
6.149AsnLeu: 6.149 ± 0.123
1.546AsnMet: 1.546 ± 0.062
3.182AsnAsn: 3.182 ± 0.107
2.201AsnPro: 2.201 ± 0.066
1.267AsnGln: 1.267 ± 0.053
2.166AsnArg: 2.166 ± 0.066
3.909AsnSer: 3.909 ± 0.102
2.91AsnThr: 2.91 ± 0.075
3.163AsnVal: 3.163 ± 0.081
0.35AsnTrp: 0.35 ± 0.028
2.91AsnTyr: 2.91 ± 0.082
0.0AsnXaa: 0.0 ± 0.0
Pro
1.727ProAla: 1.727 ± 0.07
0.238ProCys: 0.238 ± 0.027
1.702ProAsp: 1.702 ± 0.072
2.667ProGlu: 2.667 ± 0.104
1.261ProPhe: 1.261 ± 0.059
1.999ProGly: 1.999 ± 0.073
0.455ProHis: 0.455 ± 0.03
2.665ProIle: 2.665 ± 0.068
2.332ProLys: 2.332 ± 0.082
2.404ProLeu: 2.404 ± 0.076
0.827ProMet: 0.827 ± 0.041
1.295ProAsn: 1.295 ± 0.047
0.654ProPro: 0.654 ± 0.042
0.833ProGln: 0.833 ± 0.046
0.875ProArg: 0.875 ± 0.042
1.541ProSer: 1.541 ± 0.056
1.562ProThr: 1.562 ± 0.056
2.208ProVal: 2.208 ± 0.072
0.221ProTrp: 0.221 ± 0.022
1.219ProTyr: 1.219 ± 0.051
0.0ProXaa: 0.0 ± 0.0
Gln
1.402GlnAla: 1.402 ± 0.062
0.181GlnCys: 0.181 ± 0.022
1.204GlnAsp: 1.204 ± 0.053
1.733GlnGlu: 1.733 ± 0.057
0.818GlnPhe: 0.818 ± 0.043
1.512GlnGly: 1.512 ± 0.059
0.274GlnHis: 0.274 ± 0.024
2.391GlnIle: 2.391 ± 0.08
2.054GlnLys: 2.054 ± 0.063
1.832GlnLeu: 1.832 ± 0.052
0.734GlnMet: 0.734 ± 0.038
1.409GlnAsn: 1.409 ± 0.055
0.58GlnPro: 0.58 ± 0.038
0.546GlnGln: 0.546 ± 0.041
1.267GlnArg: 1.267 ± 0.055
1.421GlnSer: 1.421 ± 0.055
1.069GlnThr: 1.069 ± 0.046
1.493GlnVal: 1.493 ± 0.06
0.16GlnTrp: 0.16 ± 0.019
0.797GlnTyr: 0.797 ± 0.037
0.0GlnXaa: 0.0 ± 0.0
Arg
2.446ArgAla: 2.446 ± 0.064
0.304ArgCys: 0.304 ± 0.026
2.421ArgAsp: 2.421 ± 0.078
3.296ArgGlu: 3.296 ± 0.088
1.653ArgPhe: 1.653 ± 0.062
2.467ArgGly: 2.467 ± 0.07
0.447ArgHis: 0.447 ± 0.032
3.342ArgIle: 3.342 ± 0.083
3.051ArgLys: 3.051 ± 0.077
2.874ArgLeu: 2.874 ± 0.082
0.892ArgMet: 0.892 ± 0.041
1.967ArgAsn: 1.967 ± 0.066
1.077ArgPro: 1.077 ± 0.05
1.033ArgGln: 1.033 ± 0.044
1.638ArgArg: 1.638 ± 0.064
1.818ArgSer: 1.818 ± 0.061
1.7ArgThr: 1.7 ± 0.063
2.387ArgVal: 2.387 ± 0.074
0.188ArgTrp: 0.188 ± 0.021
1.484ArgTyr: 1.484 ± 0.06
0.0ArgXaa: 0.0 ± 0.0
Ser
3.844SerAla: 3.844 ± 0.095
0.487SerCys: 0.487 ± 0.033
3.688SerAsp: 3.688 ± 0.101
4.616SerGlu: 4.616 ± 0.115
2.95SerPhe: 2.95 ± 0.08
4.561SerGly: 4.561 ± 0.097
0.911SerHis: 0.911 ± 0.044
5.759SerIle: 5.759 ± 0.108
5.626SerLys: 5.626 ± 0.127
5.818SerLeu: 5.818 ± 0.111
1.664SerMet: 1.664 ± 0.059
3.148SerAsn: 3.148 ± 0.09
1.719SerPro: 1.719 ± 0.059
1.607SerGln: 1.607 ± 0.057
2.305SerArg: 2.305 ± 0.078
4.095SerSer: 4.095 ± 0.088
3.024SerThr: 3.024 ± 0.093
4.097SerVal: 4.097 ± 0.106
0.367SerTrp: 0.367 ± 0.028
2.537SerTyr: 2.537 ± 0.083
0.0SerXaa: 0.0 ± 0.0
Thr
3.195ThrAla: 3.195 ± 0.1
0.409ThrCys: 0.409 ± 0.031
2.92ThrAsp: 2.92 ± 0.078
3.389ThrGlu: 3.389 ± 0.099
2.121ThrPhe: 2.121 ± 0.068
3.766ThrGly: 3.766 ± 0.099
0.719ThrHis: 0.719 ± 0.04
4.293ThrIle: 4.293 ± 0.095
3.494ThrLys: 3.494 ± 0.078
4.418ThrLeu: 4.418 ± 0.108
1.111ThrMet: 1.111 ± 0.05
2.296ThrAsn: 2.296 ± 0.064
1.868ThrPro: 1.868 ± 0.063
1.28ThrGln: 1.28 ± 0.057
1.605ThrArg: 1.605 ± 0.062
2.963ThrSer: 2.963 ± 0.082
2.623ThrThr: 2.623 ± 0.078
3.583ThrVal: 3.583 ± 0.093
0.295ThrTrp: 0.295 ± 0.024
1.811ThrTyr: 1.811 ± 0.065
0.0ThrXaa: 0.0 ± 0.0
Val
4.076ValAla: 4.076 ± 0.118
0.616ValCys: 0.616 ± 0.038
4.308ValAsp: 4.308 ± 0.082
5.012ValGlu: 5.012 ± 0.122
3.133ValPhe: 3.133 ± 0.093
4.302ValGly: 4.302 ± 0.116
0.869ValHis: 0.869 ± 0.045
5.55ValIle: 5.55 ± 0.117
5.276ValLys: 5.276 ± 0.111
5.969ValLeu: 5.969 ± 0.121
1.624ValMet: 1.624 ± 0.057
3.285ValAsn: 3.285 ± 0.089
1.961ValPro: 1.961 ± 0.076
1.409ValGln: 1.409 ± 0.056
2.064ValArg: 2.064 ± 0.067
4.179ValSer: 4.179 ± 0.093
3.121ValThr: 3.121 ± 0.102
4.793ValVal: 4.793 ± 0.126
0.306ValTrp: 0.306 ± 0.024
2.419ValTyr: 2.419 ± 0.077
0.0ValXaa: 0.0 ± 0.0
Trp
0.373TrpAla: 0.373 ± 0.033
0.046TrpCys: 0.046 ± 0.01
0.352TrpAsp: 0.352 ± 0.027
0.382TrpGlu: 0.382 ± 0.03
0.207TrpPhe: 0.207 ± 0.023
0.392TrpGly: 0.392 ± 0.029
0.091TrpHis: 0.091 ± 0.014
0.493TrpIle: 0.493 ± 0.031
0.386TrpLys: 0.386 ± 0.03
0.401TrpLeu: 0.401 ± 0.032
0.202TrpMet: 0.202 ± 0.021
0.354TrpAsn: 0.354 ± 0.026
0.124TrpPro: 0.124 ± 0.016
0.181TrpGln: 0.181 ± 0.018
0.213TrpArg: 0.213 ± 0.023
0.333TrpSer: 0.333 ± 0.033
0.276TrpThr: 0.276 ± 0.027
0.312TrpVal: 0.312 ± 0.023
0.038TrpTrp: 0.038 ± 0.008
0.234TrpTyr: 0.234 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.248TyrAla: 2.248 ± 0.069
0.409TyrCys: 0.409 ± 0.033
2.792TyrAsp: 2.792 ± 0.091
3.403TyrGlu: 3.403 ± 0.092
2.056TyrPhe: 2.056 ± 0.072
2.773TyrGly: 2.773 ± 0.073
0.54TyrHis: 0.54 ± 0.036
3.739TyrIle: 3.739 ± 0.086
3.629TyrLys: 3.629 ± 0.094
3.933TyrLeu: 3.933 ± 0.098
0.953TyrMet: 0.953 ± 0.043
2.467TyrAsn: 2.467 ± 0.086
1.309TyrPro: 1.309 ± 0.05
0.816TyrGln: 0.816 ± 0.042
1.729TyrArg: 1.729 ± 0.063
2.659TyrSer: 2.659 ± 0.082
1.957TyrThr: 1.957 ± 0.065
2.381TyrVal: 2.381 ± 0.071
0.236TyrTrp: 0.236 ± 0.023
1.936TyrTyr: 1.936 ± 0.082
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1528 proteins (474248 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski