Amino acid dipepetide frequency for Streptomyces sp. HGB0020

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.308AlaAla: 20.308 ± 0.142	1.034AlaCys: 1.034 ± 0.018	8.177AlaAsp: 8.177 ± 0.063	8.429AlaGlu: 8.429 ± 0.09	3.525AlaPhe: 3.525 ± 0.038	12.324AlaGly: 12.324 ± 0.082	2.989AlaHis: 2.989 ± 0.038	3.51AlaIle: 3.51 ± 0.044	2.965AlaLys: 2.965 ± 0.048	14.248AlaLeu: 14.248 ± 0.103	2.518AlaMet: 2.518 ± 0.029	1.965AlaAsn: 1.965 ± 0.031	6.822AlaPro: 6.822 ± 0.066	3.977AlaGln: 3.977 ± 0.045	10.045AlaArg: 10.045 ± 0.075	6.141AlaSer: 6.141 ± 0.051	6.975AlaThr: 6.975 ± 0.052	11.984AlaVal: 11.984 ± 0.069	1.844AlaTrp: 1.844 ± 0.028	2.798AlaTyr: 2.798 ± 0.035	0.0AlaXaa: 0.0 ± 0.0
Cys	1.06CysAla: 1.06 ± 0.022	0.096CysCys: 0.096 ± 0.006	0.463CysAsp: 0.463 ± 0.015	0.409CysGlu: 0.409 ± 0.012	0.216CysPhe: 0.216 ± 0.008	0.935CysGly: 0.935 ± 0.019	0.197CysHis: 0.197 ± 0.008	0.189CysIle: 0.189 ± 0.008	0.118CysLys: 0.118 ± 0.007	0.755CysLeu: 0.755 ± 0.019	0.124CysMet: 0.124 ± 0.006	0.142CysAsn: 0.142 ± 0.007	0.447CysPro: 0.447 ± 0.014	0.161CysGln: 0.161 ± 0.008	0.595CysArg: 0.595 ± 0.017	0.446CysSer: 0.446 ± 0.012	0.55CysThr: 0.55 ± 0.014	0.648CysVal: 0.648 ± 0.015	0.122CysTrp: 0.122 ± 0.007	0.169CysTyr: 0.169 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.672AspAla: 7.672 ± 0.06	0.426AspCys: 0.426 ± 0.014	3.7AspAsp: 3.7 ± 0.042	3.937AspGlu: 3.937 ± 0.039	1.695AspPhe: 1.695 ± 0.022	6.34AspGly: 6.34 ± 0.063	1.456AspHis: 1.456 ± 0.024	1.96AspIle: 1.96 ± 0.026	1.29AspLys: 1.29 ± 0.027	6.253AspLeu: 6.253 ± 0.045	0.868AspMet: 0.868 ± 0.017	1.06AspAsn: 1.06 ± 0.023	4.419AspPro: 4.419 ± 0.042	1.562AspGln: 1.562 ± 0.024	4.813AspArg: 4.813 ± 0.042	2.603AspSer: 2.603 ± 0.031	3.282AspThr: 3.282 ± 0.038	4.909AspVal: 4.909 ± 0.044	1.083AspTrp: 1.083 ± 0.021	1.149AspTyr: 1.149 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	7.21GluAla: 7.21 ± 0.068	0.368GluCys: 0.368 ± 0.013	2.835GluAsp: 2.835 ± 0.035	3.537GluGlu: 3.537 ± 0.053	1.492GluPhe: 1.492 ± 0.026	4.282GluGly: 4.282 ± 0.041	1.516GluHis: 1.516 ± 0.025	2.246GluIle: 2.246 ± 0.034	1.46GluLys: 1.46 ± 0.031	6.689GluLeu: 6.689 ± 0.061	0.892GluMet: 0.892 ± 0.015	1.043GluAsn: 1.043 ± 0.021	3.355GluPro: 3.355 ± 0.041	2.372GluGln: 2.372 ± 0.035	5.374GluArg: 5.374 ± 0.054	2.598GluSer: 2.598 ± 0.034	2.872GluThr: 2.872 ± 0.031	4.373GluVal: 4.373 ± 0.046	0.773GluTrp: 0.773 ± 0.016	1.126GluTyr: 1.126 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.66PheAla: 3.66 ± 0.041	0.278PheCys: 0.278 ± 0.009	1.963PheAsp: 1.963 ± 0.032	1.471PheGlu: 1.471 ± 0.025	0.905PhePhe: 0.905 ± 0.02	3.06PheGly: 3.06 ± 0.033	0.629PheHis: 0.629 ± 0.016	0.725PheIle: 0.725 ± 0.016	0.543PheLys: 0.543 ± 0.015	2.619PheLeu: 2.619 ± 0.035	0.435PheMet: 0.435 ± 0.011	0.603PheAsn: 0.603 ± 0.016	1.413PhePro: 1.413 ± 0.023	0.7PheGln: 0.7 ± 0.018	1.854PheArg: 1.854 ± 0.029	1.468PheSer: 1.468 ± 0.023	2.081PheThr: 2.081 ± 0.03	2.352PheVal: 2.352 ± 0.037	0.426PheTrp: 0.426 ± 0.014	0.611PheTyr: 0.611 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	10.777GlyAla: 10.777 ± 0.072	0.834GlyCys: 0.834 ± 0.014	5.093GlyAsp: 5.093 ± 0.046	5.007GlyGlu: 5.007 ± 0.048	2.904GlyPhe: 2.904 ± 0.03	8.553GlyGly: 8.553 ± 0.066	2.382GlyHis: 2.382 ± 0.034	3.56GlyIle: 3.56 ± 0.037	2.457GlyLys: 2.457 ± 0.039	9.321GlyLeu: 9.321 ± 0.069	1.992GlyMet: 1.992 ± 0.027	1.805GlyAsn: 1.805 ± 0.032	5.015GlyPro: 5.015 ± 0.046	2.67GlyGln: 2.67 ± 0.03	7.603GlyArg: 7.603 ± 0.066	5.492GlySer: 5.492 ± 0.056	6.337GlyThr: 6.337 ± 0.053	7.397GlyVal: 7.397 ± 0.063	1.689GlyTrp: 1.689 ± 0.028	2.291GlyTyr: 2.291 ± 0.027	0.0GlyXaa: 0.0 ± 0.0
His	2.733HisAla: 2.733 ± 0.036	0.22HisCys: 0.22 ± 0.008	1.407HisAsp: 1.407 ± 0.022	1.298HisGlu: 1.298 ± 0.025	0.685HisPhe: 0.685 ± 0.018	2.488HisGly: 2.488 ± 0.031	0.725HisHis: 0.725 ± 0.019	0.753HisIle: 0.753 ± 0.017	0.398HisLys: 0.398 ± 0.011	2.465HisLeu: 2.465 ± 0.034	0.361HisMet: 0.361 ± 0.012	0.397HisAsn: 0.397 ± 0.011	1.837HisPro: 1.837 ± 0.027	0.636HisGln: 0.636 ± 0.015	2.124HisArg: 2.124 ± 0.031	1.052HisSer: 1.052 ± 0.021	1.432HisThr: 1.432 ± 0.026	1.758HisVal: 1.758 ± 0.026	0.395HisTrp: 0.395 ± 0.011	0.521HisTyr: 0.521 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.879IleAla: 4.879 ± 0.042	0.296IleCys: 0.296 ± 0.01	2.285IleAsp: 2.285 ± 0.034	1.989IleGlu: 1.989 ± 0.031	0.735IlePhe: 0.735 ± 0.018	3.584IleGly: 3.584 ± 0.042	0.632IleHis: 0.632 ± 0.015	0.882IleIle: 0.882 ± 0.02	0.781IleLys: 0.781 ± 0.018	2.473IleLeu: 2.473 ± 0.035	0.465IleMet: 0.465 ± 0.016	0.723IleAsn: 0.723 ± 0.017	1.797IlePro: 1.797 ± 0.023	0.77IleGln: 0.77 ± 0.019	2.288IleArg: 2.288 ± 0.029	1.748IleSer: 1.748 ± 0.027	2.285IleThr: 2.285 ± 0.029	2.84IleVal: 2.84 ± 0.034	0.401IleTrp: 0.401 ± 0.014	0.571IleTyr: 0.571 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.062LysAla: 3.062 ± 0.04	0.112LysCys: 0.112 ± 0.007	1.449LysAsp: 1.449 ± 0.027	1.258LysGlu: 1.258 ± 0.028	0.482LysPhe: 0.482 ± 0.014	2.025LysGly: 2.025 ± 0.036	0.439LysHis: 0.439 ± 0.013	0.883LysIle: 0.883 ± 0.022	0.996LysLys: 0.996 ± 0.028	2.045LysLeu: 2.045 ± 0.032	0.392LysMet: 0.392 ± 0.014	0.572LysAsn: 0.572 ± 0.017	1.431LysPro: 1.431 ± 0.036	0.787LysGln: 0.787 ± 0.019	1.405LysArg: 1.405 ± 0.025	1.211LysSer: 1.211 ± 0.023	1.364LysThr: 1.364 ± 0.027	2.019LysVal: 2.019 ± 0.037	0.297LysTrp: 0.297 ± 0.011	0.515LysTyr: 0.515 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	14.661LeuAla: 14.661 ± 0.108	0.862LeuCys: 0.862 ± 0.02	6.767LeuAsp: 6.767 ± 0.056	4.613LeuGlu: 4.613 ± 0.053	2.622LeuPhe: 2.622 ± 0.036	9.181LeuGly: 9.181 ± 0.062	2.394LeuHis: 2.394 ± 0.031	3.338LeuIle: 3.338 ± 0.039	2.171LeuLys: 2.171 ± 0.033	11.238LeuLeu: 11.238 ± 0.095	1.672LeuMet: 1.672 ± 0.025	1.744LeuAsn: 1.744 ± 0.028	6.336LeuPro: 6.336 ± 0.053	2.418LeuGln: 2.418 ± 0.03	8.383LeuArg: 8.383 ± 0.064	5.3LeuSer: 5.3 ± 0.046	6.981LeuThr: 6.981 ± 0.055	8.876LeuVal: 8.876 ± 0.068	1.306LeuTrp: 1.306 ± 0.026	1.967LeuTyr: 1.967 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.254MetAla: 2.254 ± 0.03	0.145MetCys: 0.145 ± 0.007	0.89MetAsp: 0.89 ± 0.017	0.74MetGlu: 0.74 ± 0.017	0.472MetPhe: 0.472 ± 0.013	1.329MetGly: 1.329 ± 0.026	0.393MetHis: 0.393 ± 0.012	0.687MetIle: 0.687 ± 0.016	0.44MetLys: 0.44 ± 0.013	1.703MetLeu: 1.703 ± 0.026	0.294MetMet: 0.294 ± 0.01	0.459MetAsn: 0.459 ± 0.014	1.128MetPro: 1.128 ± 0.023	0.485MetGln: 0.485 ± 0.014	1.413MetArg: 1.413 ± 0.024	1.383MetSer: 1.383 ± 0.025	1.607MetThr: 1.607 ± 0.022	1.247MetVal: 1.247 ± 0.023	0.223MetTrp: 0.223 ± 0.01	0.331MetTyr: 0.331 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.261AsnAla: 2.261 ± 0.031	0.168AsnCys: 0.168 ± 0.007	1.024AsnAsp: 1.024 ± 0.019	0.822AsnGlu: 0.822 ± 0.019	0.519AsnPhe: 0.519 ± 0.016	1.983AsnGly: 1.983 ± 0.032	0.422AsnHis: 0.422 ± 0.014	0.667AsnIle: 0.667 ± 0.017	0.447AsnLys: 0.447 ± 0.012	1.704AsnLeu: 1.704 ± 0.026	0.314AsnMet: 0.314 ± 0.01	0.466AsnAsn: 0.466 ± 0.016	1.406AsnPro: 1.406 ± 0.024	0.521AsnGln: 0.521 ± 0.014	1.302AsnArg: 1.302 ± 0.023	1.05AsnSer: 1.05 ± 0.02	1.156AsnThr: 1.156 ± 0.025	1.414AsnVal: 1.414 ± 0.026	0.316AsnTrp: 0.316 ± 0.011	0.448AsnTyr: 0.448 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	8.192ProAla: 8.192 ± 0.073	0.318ProCys: 0.318 ± 0.012	4.496ProAsp: 4.496 ± 0.041	4.223ProGlu: 4.223 ± 0.044	1.529ProPhe: 1.529 ± 0.026	6.324ProGly: 6.324 ± 0.063	1.473ProHis: 1.473 ± 0.028	1.297ProIle: 1.297 ± 0.023	1.272ProLys: 1.272 ± 0.025	5.313ProLeu: 5.313 ± 0.051	0.994ProMet: 0.994 ± 0.018	0.945ProAsn: 0.945 ± 0.021	3.441ProPro: 3.441 ± 0.052	1.88ProGln: 1.88 ± 0.037	3.789ProArg: 3.789 ± 0.043	3.28ProSer: 3.28 ± 0.044	3.261ProThr: 3.261 ± 0.037	5.468ProVal: 5.468 ± 0.057	0.904ProTrp: 0.904 ± 0.022	1.456ProTyr: 1.456 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	3.739GlnAla: 3.739 ± 0.04	0.191GlnCys: 0.191 ± 0.008	1.473GlnAsp: 1.473 ± 0.022	1.501GlnGlu: 1.501 ± 0.023	0.757GlnPhe: 0.757 ± 0.019	2.442GlnGly: 2.442 ± 0.033	0.711GlnHis: 0.711 ± 0.018	1.14GlnIle: 1.14 ± 0.019	0.696GlnLys: 0.696 ± 0.018	3.228GlnLeu: 3.228 ± 0.03	0.534GlnMet: 0.534 ± 0.014	0.548GlnAsn: 0.548 ± 0.014	1.787GlnPro: 1.787 ± 0.035	1.354GlnGln: 1.354 ± 0.034	2.39GlnArg: 2.39 ± 0.037	1.427GlnSer: 1.427 ± 0.024	1.435GlnThr: 1.435 ± 0.022	2.42GlnVal: 2.42 ± 0.03	0.492GlnTrp: 0.492 ± 0.013	0.663GlnTyr: 0.663 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	9.569ArgAla: 9.569 ± 0.073	0.551ArgCys: 0.551 ± 0.015	4.134ArgAsp: 4.134 ± 0.043	4.653ArgGlu: 4.653 ± 0.046	2.321ArgPhe: 2.321 ± 0.035	5.615ArgGly: 5.615 ± 0.052	2.149ArgHis: 2.149 ± 0.03	3.348ArgIle: 3.348 ± 0.036	1.67ArgLys: 1.67 ± 0.029	8.732ArgLeu: 8.732 ± 0.07	1.682ArgMet: 1.682 ± 0.027	1.346ArgAsn: 1.346 ± 0.026	4.812ArgPro: 4.812 ± 0.048	2.331ArgGln: 2.331 ± 0.032	7.611ArgArg: 7.611 ± 0.075	4.064ArgSer: 4.064 ± 0.045	5.474ArgThr: 5.474 ± 0.053	5.78ArgVal: 5.78 ± 0.058	1.329ArgTrp: 1.329 ± 0.025	1.763ArgTyr: 1.763 ± 0.025	0.0ArgXaa: 0.0 ± 0.0
Ser	6.895SerAla: 6.895 ± 0.058	0.41SerCys: 0.41 ± 0.013	2.858SerAsp: 2.858 ± 0.035	2.476SerGlu: 2.476 ± 0.035	1.562SerPhe: 1.562 ± 0.022	6.047SerGly: 6.047 ± 0.053	1.046SerHis: 1.046 ± 0.021	1.48SerIle: 1.48 ± 0.023	1.141SerLys: 1.141 ± 0.023	4.878SerLeu: 4.878 ± 0.041	1.11SerMet: 1.11 ± 0.02	0.951SerAsn: 0.951 ± 0.019	3.183SerPro: 3.183 ± 0.039	1.318SerGln: 1.318 ± 0.022	3.7SerArg: 3.7 ± 0.038	3.01SerSer: 3.01 ± 0.047	3.204SerThr: 3.204 ± 0.033	4.411SerVal: 4.411 ± 0.044	0.969SerTrp: 0.969 ± 0.021	1.357SerTyr: 1.357 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	8.734ThrAla: 8.734 ± 0.06	0.441ThrCys: 0.441 ± 0.015	3.876ThrAsp: 3.876 ± 0.043	3.262ThrGlu: 3.262 ± 0.036	1.673ThrPhe: 1.673 ± 0.022	6.664ThrGly: 6.664 ± 0.055	1.305ThrHis: 1.305 ± 0.02	1.784ThrIle: 1.784 ± 0.03	1.284ThrLys: 1.284 ± 0.025	5.8ThrLeu: 5.8 ± 0.052	0.949ThrMet: 0.949 ± 0.02	1.058ThrAsn: 1.058 ± 0.025	4.192ThrPro: 4.192 ± 0.049	1.456ThrGln: 1.456 ± 0.024	3.941ThrArg: 3.941 ± 0.037	3.33ThrSer: 3.33 ± 0.038	4.034ThrThr: 4.034 ± 0.043	6.111ThrVal: 6.111 ± 0.046	0.951ThrTrp: 0.951 ± 0.018	1.441ThrTyr: 1.441 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	10.451ValAla: 10.451 ± 0.075	0.756ValCys: 0.756 ± 0.018	5.042ValAsp: 5.042 ± 0.044	4.709ValGlu: 4.709 ± 0.051	2.464ValPhe: 2.464 ± 0.028	6.525ValGly: 6.525 ± 0.061	2.044ValHis: 2.044 ± 0.03	2.86ValIle: 2.86 ± 0.037	1.808ValLys: 1.808 ± 0.028	9.495ValLeu: 9.495 ± 0.081	1.452ValMet: 1.452 ± 0.024	1.73ValAsn: 1.73 ± 0.028	5.165ValPro: 5.165 ± 0.046	2.241ValGln: 2.241 ± 0.03	7.149ValArg: 7.149 ± 0.063	4.424ValSer: 4.424 ± 0.044	5.681ValThr: 5.681 ± 0.052	8.101ValVal: 8.101 ± 0.075	1.189ValTrp: 1.189 ± 0.021	1.615ValTyr: 1.615 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.642TrpAla: 1.642 ± 0.027	0.153TrpCys: 0.153 ± 0.008	0.862TrpAsp: 0.862 ± 0.02	0.717TrpGlu: 0.717 ± 0.016	0.528TrpPhe: 0.528 ± 0.015	1.081TrpGly: 1.081 ± 0.022	0.376TrpHis: 0.376 ± 0.012	0.587TrpIle: 0.587 ± 0.014	0.372TrpLys: 0.372 ± 0.012	1.792TrpLeu: 1.792 ± 0.023	0.303TrpMet: 0.303 ± 0.012	0.442TrpAsn: 0.442 ± 0.014	0.786TrpPro: 0.786 ± 0.017	0.678TrpGln: 0.678 ± 0.016	1.336TrpArg: 1.336 ± 0.025	0.977TrpSer: 0.977 ± 0.018	1.046TrpThr: 1.046 ± 0.021	1.016TrpVal: 1.016 ± 0.022	0.355TrpTrp: 0.355 ± 0.013	0.377TrpTyr: 0.377 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.817TyrAla: 2.817 ± 0.033	0.184TyrCys: 0.184 ± 0.009	1.682TyrAsp: 1.682 ± 0.032	1.362TyrGlu: 1.362 ± 0.024	0.707TyrPhe: 0.707 ± 0.018	2.395TyrGly: 2.395 ± 0.037	0.409TyrHis: 0.409 ± 0.013	0.528TyrIle: 0.528 ± 0.014	0.434TyrLys: 0.434 ± 0.012	2.079TyrLeu: 2.079 ± 0.03	0.272TyrMet: 0.272 ± 0.01	0.411TyrAsn: 0.411 ± 0.013	1.083TyrPro: 1.083 ± 0.02	0.612TyrGln: 0.612 ± 0.018	1.82TyrArg: 1.82 ± 0.03	1.001TyrSer: 1.001 ± 0.02	1.232TyrThr: 1.232 ± 0.023	1.78TyrVal: 1.78 ± 0.027	0.363TyrTrp: 0.363 ± 0.012	0.488TyrTyr: 0.488 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8369 proteins (2744775 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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