Amino acid dipepetide frequency for Flavobacterium sp. HMWF030

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.444AlaAla: 4.444 ± 0.074	0.522AlaCys: 0.522 ± 0.019	3.411AlaAsp: 3.411 ± 0.059	3.967AlaGlu: 3.967 ± 0.063	3.386AlaPhe: 3.386 ± 0.053	4.3AlaGly: 4.3 ± 0.069	0.995AlaHis: 0.995 ± 0.029	5.51AlaIle: 5.51 ± 0.069	5.159AlaLys: 5.159 ± 0.067	6.321AlaLeu: 6.321 ± 0.082	1.491AlaMet: 1.491 ± 0.037	3.769AlaAsn: 3.769 ± 0.057	1.935AlaPro: 1.935 ± 0.049	2.498AlaGln: 2.498 ± 0.045	1.834AlaArg: 1.834 ± 0.039	4.378AlaSer: 4.378 ± 0.063	3.938AlaThr: 3.938 ± 0.066	4.264AlaVal: 4.264 ± 0.059	0.628AlaTrp: 0.628 ± 0.022	2.434AlaTyr: 2.434 ± 0.042	0.001AlaXaa: 0.001 ± 0.001
Cys	0.439CysAla: 0.439 ± 0.019	0.105CysCys: 0.105 ± 0.008	0.428CysAsp: 0.428 ± 0.019	0.419CysGlu: 0.419 ± 0.015	0.434CysPhe: 0.434 ± 0.019	0.621CysGly: 0.621 ± 0.023	0.178CysHis: 0.178 ± 0.015	0.647CysIle: 0.647 ± 0.024	0.488CysLys: 0.488 ± 0.024	0.694CysLeu: 0.694 ± 0.023	0.148CysMet: 0.148 ± 0.011	0.411CysAsn: 0.411 ± 0.019	0.32CysPro: 0.32 ± 0.018	0.202CysGln: 0.202 ± 0.014	0.213CysArg: 0.213 ± 0.011	0.599CysSer: 0.599 ± 0.025	0.445CysThr: 0.445 ± 0.018	0.448CysVal: 0.448 ± 0.021	0.072CysTrp: 0.072 ± 0.008	0.303CysTyr: 0.303 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.649AspAla: 3.649 ± 0.061	0.42AspCys: 0.42 ± 0.02	2.675AspAsp: 2.675 ± 0.05	3.389AspGlu: 3.389 ± 0.063	3.687AspPhe: 3.687 ± 0.062	3.495AspGly: 3.495 ± 0.064	0.851AspHis: 0.851 ± 0.025	4.073AspIle: 4.073 ± 0.062	4.443AspLys: 4.443 ± 0.074	5.401AspLeu: 5.401 ± 0.071	0.987AspMet: 0.987 ± 0.03	3.122AspAsn: 3.122 ± 0.051	1.758AspPro: 1.758 ± 0.039	1.642AspGln: 1.642 ± 0.036	1.751AspArg: 1.751 ± 0.037	3.059AspSer: 3.059 ± 0.056	2.484AspThr: 2.484 ± 0.039	3.321AspVal: 3.321 ± 0.054	0.773AspTrp: 0.773 ± 0.024	2.724AspTyr: 2.724 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	4.009GluAla: 4.009 ± 0.064	0.334GluCys: 0.334 ± 0.015	3.085GluAsp: 3.085 ± 0.054	4.042GluGlu: 4.042 ± 0.076	2.792GluPhe: 2.792 ± 0.045	3.228GluGly: 3.228 ± 0.053	0.983GluHis: 0.983 ± 0.03	5.697GluIle: 5.697 ± 0.073	6.2GluLys: 6.2 ± 0.084	5.566GluLeu: 5.566 ± 0.072	1.551GluMet: 1.551 ± 0.032	4.869GluAsn: 4.869 ± 0.065	1.501GluPro: 1.501 ± 0.037	2.115GluGln: 2.115 ± 0.035	2.188GluArg: 2.188 ± 0.043	3.439GluSer: 3.439 ± 0.053	3.687GluThr: 3.687 ± 0.059	3.904GluVal: 3.904 ± 0.06	0.616GluTrp: 0.616 ± 0.024	2.279GluTyr: 2.279 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	3.413PheAla: 3.413 ± 0.056	0.483PheCys: 0.483 ± 0.021	3.381PheAsp: 3.381 ± 0.057	3.342PheGlu: 3.342 ± 0.056	2.897PhePhe: 2.897 ± 0.054	3.633PheGly: 3.633 ± 0.059	0.864PheHis: 0.864 ± 0.026	3.992PheIle: 3.992 ± 0.059	3.803PheLys: 3.803 ± 0.054	4.839PheLeu: 4.839 ± 0.076	1.088PheMet: 1.088 ± 0.027	3.27PheAsn: 3.27 ± 0.059	1.829PhePro: 1.829 ± 0.04	1.622PheGln: 1.622 ± 0.037	1.624PheArg: 1.624 ± 0.034	4.214PheSer: 4.214 ± 0.063	3.191PheThr: 3.191 ± 0.053	3.238PheVal: 3.238 ± 0.057	0.593PheTrp: 0.593 ± 0.022	2.296PheTyr: 2.296 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	4.0GlyAla: 4.0 ± 0.058	0.584GlyCys: 0.584 ± 0.024	3.057GlyAsp: 3.057 ± 0.056	3.184GlyGlu: 3.184 ± 0.053	3.687GlyPhe: 3.687 ± 0.055	4.268GlyGly: 4.268 ± 0.077	1.036GlyHis: 1.036 ± 0.031	5.508GlyIle: 5.508 ± 0.075	5.229GlyLys: 5.229 ± 0.071	5.649GlyLeu: 5.649 ± 0.073	1.472GlyMet: 1.472 ± 0.037	3.724GlyAsn: 3.724 ± 0.061	1.272GlyPro: 1.272 ± 0.037	1.899GlyGln: 1.899 ± 0.038	1.953GlyArg: 1.953 ± 0.042	4.053GlySer: 4.053 ± 0.065	4.049GlyThr: 4.049 ± 0.076	4.094GlyVal: 4.094 ± 0.063	0.775GlyTrp: 0.775 ± 0.025	2.831GlyTyr: 2.831 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	0.87HisAla: 0.87 ± 0.023	0.16HisCys: 0.16 ± 0.012	0.845HisAsp: 0.845 ± 0.026	0.925HisGlu: 0.925 ± 0.027	1.167HisPhe: 1.167 ± 0.03	0.939HisGly: 0.939 ± 0.03	0.447HisHis: 0.447 ± 0.02	1.305HisIle: 1.305 ± 0.033	1.124HisLys: 1.124 ± 0.03	1.652HisLeu: 1.652 ± 0.036	0.285HisMet: 0.285 ± 0.016	1.001HisAsn: 1.001 ± 0.028	0.77HisPro: 0.77 ± 0.023	0.652HisGln: 0.652 ± 0.023	0.536HisArg: 0.536 ± 0.02	1.002HisSer: 1.002 ± 0.028	0.861HisThr: 0.861 ± 0.026	0.787HisVal: 0.787 ± 0.029	0.205HisTrp: 0.205 ± 0.014	0.791HisTyr: 0.791 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.827IleAla: 5.827 ± 0.069	0.67IleCys: 0.67 ± 0.025	4.681IleAsp: 4.681 ± 0.06	5.534IleGlu: 5.534 ± 0.07	3.782IlePhe: 3.782 ± 0.062	5.032IleGly: 5.032 ± 0.069	1.316IleHis: 1.316 ± 0.033	6.37IleIle: 6.37 ± 0.087	6.255IleLys: 6.255 ± 0.079	7.355IleLeu: 7.355 ± 0.082	1.475IleMet: 1.475 ± 0.034	4.972IleAsn: 4.972 ± 0.065	3.265IlePro: 3.265 ± 0.057	2.803IleGln: 2.803 ± 0.046	2.481IleArg: 2.481 ± 0.044	5.927IleSer: 5.927 ± 0.07	5.117IleThr: 5.117 ± 0.057	5.01IleVal: 5.01 ± 0.066	0.735IleTrp: 0.735 ± 0.025	2.929IleTyr: 2.929 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	5.283LysAla: 5.283 ± 0.075	0.401LysCys: 0.401 ± 0.017	4.616LysAsp: 4.616 ± 0.064	5.928LysGlu: 5.928 ± 0.073	3.22LysPhe: 3.22 ± 0.054	4.72LysGly: 4.72 ± 0.063	1.233LysHis: 1.233 ± 0.034	7.204LysIle: 7.204 ± 0.089	7.345LysLys: 7.345 ± 0.092	6.683LysLeu: 6.683 ± 0.064	2.12LysMet: 2.12 ± 0.038	5.953LysAsn: 5.953 ± 0.068	2.69LysPro: 2.69 ± 0.046	2.723LysGln: 2.723 ± 0.048	2.54LysArg: 2.54 ± 0.054	4.863LysSer: 4.863 ± 0.063	5.229LysThr: 5.229 ± 0.075	5.001LysVal: 5.001 ± 0.065	0.913LysTrp: 0.913 ± 0.025	3.3LysTyr: 3.3 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	5.935LeuAla: 5.935 ± 0.072	0.657LeuCys: 0.657 ± 0.022	4.86LeuAsp: 4.86 ± 0.068	5.742LeuGlu: 5.742 ± 0.066	5.003LeuPhe: 5.003 ± 0.072	5.553LeuGly: 5.553 ± 0.07	1.537LeuHis: 1.537 ± 0.034	7.027LeuIle: 7.027 ± 0.084	7.973LeuLys: 7.973 ± 0.09	8.72LeuLeu: 8.72 ± 0.102	1.969LeuMet: 1.969 ± 0.042	5.787LeuAsn: 5.787 ± 0.066	3.596LeuPro: 3.596 ± 0.057	3.289LeuGln: 3.289 ± 0.049	2.917LeuArg: 2.917 ± 0.048	6.72LeuSer: 6.72 ± 0.08	5.166LeuThr: 5.166 ± 0.073	5.289LeuVal: 5.289 ± 0.065	0.807LeuTrp: 0.807 ± 0.027	3.265LeuTyr: 3.265 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	1.471MetAla: 1.471 ± 0.034	0.14MetCys: 0.14 ± 0.011	1.005MetAsp: 1.005 ± 0.028	1.223MetGlu: 1.223 ± 0.032	0.852MetPhe: 0.852 ± 0.028	1.276MetGly: 1.276 ± 0.031	0.389MetHis: 0.389 ± 0.017	1.627MetIle: 1.627 ± 0.04	2.344MetLys: 2.344 ± 0.043	1.774MetLeu: 1.774 ± 0.037	0.57MetMet: 0.57 ± 0.021	1.32MetAsn: 1.32 ± 0.031	0.808MetPro: 0.808 ± 0.025	0.811MetGln: 0.811 ± 0.028	0.774MetArg: 0.774 ± 0.024	1.454MetSer: 1.454 ± 0.029	1.152MetThr: 1.152 ± 0.031	1.198MetVal: 1.198 ± 0.031	0.167MetTrp: 0.167 ± 0.011	0.671MetTyr: 0.671 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	4.14AsnAla: 4.14 ± 0.065	0.512AsnCys: 0.512 ± 0.023	3.292AsnAsp: 3.292 ± 0.055	3.955AsnGlu: 3.955 ± 0.055	3.313AsnPhe: 3.313 ± 0.06	4.178AsnGly: 4.178 ± 0.073	1.094AsnHis: 1.094 ± 0.028	4.939AsnIle: 4.939 ± 0.057	4.818AsnLys: 4.818 ± 0.065	5.741AsnLeu: 5.741 ± 0.074	1.147AsnMet: 1.147 ± 0.029	4.084AsnAsn: 4.084 ± 0.072	2.964AsnPro: 2.964 ± 0.052	2.443AsnGln: 2.443 ± 0.042	2.02AsnArg: 2.02 ± 0.038	4.256AsnSer: 4.256 ± 0.065	3.806AsnThr: 3.806 ± 0.064	3.729AsnVal: 3.729 ± 0.065	0.844AsnTrp: 0.844 ± 0.026	2.977AsnTyr: 2.977 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	2.186ProAla: 2.186 ± 0.045	0.203ProCys: 0.203 ± 0.012	2.028ProAsp: 2.028 ± 0.045	2.721ProGlu: 2.721 ± 0.05	1.989ProPhe: 1.989 ± 0.045	1.886ProGly: 1.886 ± 0.047	0.511ProHis: 0.511 ± 0.019	2.708ProIle: 2.708 ± 0.041	2.639ProLys: 2.639 ± 0.046	2.986ProLeu: 2.986 ± 0.051	0.722ProMet: 0.722 ± 0.022	2.339ProAsn: 2.339 ± 0.051	0.766ProPro: 0.766 ± 0.027	1.136ProGln: 1.136 ± 0.034	0.896ProArg: 0.896 ± 0.028	2.129ProSer: 2.129 ± 0.046	2.12ProThr: 2.12 ± 0.046	2.446ProVal: 2.446 ± 0.048	0.332ProTrp: 0.332 ± 0.015	1.398ProTyr: 1.398 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	1.967GlnAla: 1.967 ± 0.043	0.19GlnCys: 0.19 ± 0.011	1.622GlnAsp: 1.622 ± 0.039	2.217GlnGlu: 2.217 ± 0.046	1.74GlnPhe: 1.74 ± 0.037	1.72GlnGly: 1.72 ± 0.035	0.531GlnHis: 0.531 ± 0.021	2.922GlnIle: 2.922 ± 0.047	3.298GlnLys: 3.298 ± 0.052	3.266GlnLeu: 3.266 ± 0.049	0.747GlnMet: 0.747 ± 0.027	2.638GlnAsn: 2.638 ± 0.046	1.033GlnPro: 1.033 ± 0.033	1.342GlnGln: 1.342 ± 0.035	1.137GlnArg: 1.137 ± 0.03	2.076GlnSer: 2.076 ± 0.04	2.076GlnThr: 2.076 ± 0.042	1.872GlnVal: 1.872 ± 0.034	0.349GlnTrp: 0.349 ± 0.016	1.446GlnTyr: 1.446 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	1.946ArgAla: 1.946 ± 0.043	0.205ArgCys: 0.205 ± 0.014	1.612ArgAsp: 1.612 ± 0.035	2.026ArgGlu: 2.026 ± 0.038	1.828ArgPhe: 1.828 ± 0.038	1.668ArgGly: 1.668 ± 0.039	0.501ArgHis: 0.501 ± 0.018	2.835ArgIle: 2.835 ± 0.053	2.635ArgLys: 2.635 ± 0.045	2.938ArgLeu: 2.938 ± 0.048	0.807ArgMet: 0.807 ± 0.026	2.058ArgAsn: 2.058 ± 0.042	1.042ArgPro: 1.042 ± 0.03	0.959ArgGln: 0.959 ± 0.023	1.116ArgArg: 1.116 ± 0.031	1.756ArgSer: 1.756 ± 0.033	1.756ArgThr: 1.756 ± 0.04	1.837ArgVal: 1.837 ± 0.038	0.368ArgTrp: 0.368 ± 0.017	1.436ArgTyr: 1.436 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	4.047SerAla: 4.047 ± 0.064	0.682SerCys: 0.682 ± 0.026	3.546SerAsp: 3.546 ± 0.049	3.738SerGlu: 3.738 ± 0.053	4.058SerPhe: 4.058 ± 0.064	4.672SerGly: 4.672 ± 0.072	1.078SerHis: 1.078 ± 0.033	5.459SerIle: 5.459 ± 0.067	5.231SerLys: 5.231 ± 0.068	6.112SerLeu: 6.112 ± 0.081	1.262SerMet: 1.262 ± 0.034	4.154SerAsn: 4.154 ± 0.069	2.126SerPro: 2.126 ± 0.037	2.337SerGln: 2.337 ± 0.04	2.026SerArg: 2.026 ± 0.04	4.378SerSer: 4.378 ± 0.078	3.723SerThr: 3.723 ± 0.062	4.002SerVal: 4.002 ± 0.057	0.74SerTrp: 0.74 ± 0.026	2.923SerTyr: 2.923 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	4.251ThrAla: 4.251 ± 0.066	0.384ThrCys: 0.384 ± 0.019	3.25ThrAsp: 3.25 ± 0.052	3.322ThrGlu: 3.322 ± 0.053	3.18ThrPhe: 3.18 ± 0.049	4.133ThrGly: 4.133 ± 0.071	0.913ThrHis: 0.913 ± 0.025	5.244ThrIle: 5.244 ± 0.066	4.231ThrLys: 4.231 ± 0.056	5.315ThrLeu: 5.315 ± 0.07	0.96ThrMet: 0.96 ± 0.026	3.546ThrAsn: 3.546 ± 0.058	2.542ThrPro: 2.542 ± 0.051	1.911ThrGln: 1.911 ± 0.04	1.587ThrArg: 1.587 ± 0.037	3.869ThrSer: 3.869 ± 0.067	3.908ThrThr: 3.908 ± 0.076	3.926ThrVal: 3.926 ± 0.076	0.585ThrTrp: 0.585 ± 0.024	2.324ThrTyr: 2.324 ± 0.05	0.001ThrXaa: 0.001 ± 0.001
Val	4.214ValAla: 4.214 ± 0.055	0.527ValCys: 0.527 ± 0.018	3.292ValAsp: 3.292 ± 0.048	3.479ValGlu: 3.479 ± 0.052	3.441ValPhe: 3.441 ± 0.054	3.722ValGly: 3.722 ± 0.055	0.917ValHis: 0.917 ± 0.025	4.823ValIle: 4.823 ± 0.072	4.665ValLys: 4.665 ± 0.059	5.986ValLeu: 5.986 ± 0.068	1.262ValMet: 1.262 ± 0.033	3.701ValAsn: 3.701 ± 0.063	2.197ValPro: 2.197 ± 0.042	1.834ValGln: 1.834 ± 0.035	1.879ValArg: 1.879 ± 0.04	4.558ValSer: 4.558 ± 0.071	3.671ValThr: 3.671 ± 0.072	4.112ValVal: 4.112 ± 0.061	0.605ValTrp: 0.605 ± 0.02	2.42ValTyr: 2.42 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.631TrpAla: 0.631 ± 0.024	0.107TrpCys: 0.107 ± 0.008	0.615TrpAsp: 0.615 ± 0.022	0.66TrpGlu: 0.66 ± 0.023	0.597TrpPhe: 0.597 ± 0.025	0.687TrpGly: 0.687 ± 0.025	0.222TrpHis: 0.222 ± 0.014	0.79TrpIle: 0.79 ± 0.022	0.885TrpLys: 0.885 ± 0.03	0.96TrpLeu: 0.96 ± 0.031	0.274TrpMet: 0.274 ± 0.014	0.767TrpAsn: 0.767 ± 0.025	0.248TrpPro: 0.248 ± 0.015	0.411TrpGln: 0.411 ± 0.02	0.403TrpArg: 0.403 ± 0.02	0.68TrpSer: 0.68 ± 0.025	0.622TrpThr: 0.622 ± 0.026	0.578TrpVal: 0.578 ± 0.022	0.169TrpTrp: 0.169 ± 0.012	0.46TrpTyr: 0.46 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.462TyrAla: 2.462 ± 0.043	0.327TyrCys: 0.327 ± 0.016	2.402TyrAsp: 2.402 ± 0.042	2.272TyrGlu: 2.272 ± 0.042	2.56TyrPhe: 2.56 ± 0.049	2.596TyrGly: 2.596 ± 0.054	0.728TyrHis: 0.728 ± 0.026	2.856TyrIle: 2.856 ± 0.051	3.202TyrLys: 3.202 ± 0.057	3.853TyrLeu: 3.853 ± 0.05	0.689TyrMet: 0.689 ± 0.021	2.767TyrAsn: 2.767 ± 0.046	1.478TyrPro: 1.478 ± 0.029	1.56TyrGln: 1.56 ± 0.035	1.451TyrArg: 1.451 ± 0.034	2.884TyrSer: 2.884 ± 0.047	2.381TyrThr: 2.381 ± 0.05	2.247TyrVal: 2.247 ± 0.041	0.49TyrTrp: 0.49 ± 0.019	1.944TyrTyr: 1.944 ± 0.042	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.004XaaXaa: 0.004 ± 0.002

Statistics based on 4740 proteins (1334036 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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