Amino acid dipepetide frequency for Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.556AlaAla: 4.556 ± 0.084	0.702AlaCys: 0.702 ± 0.03	2.75AlaAsp: 2.75 ± 0.059	3.412AlaGlu: 3.412 ± 0.067	2.478AlaPhe: 2.478 ± 0.053	3.755AlaGly: 3.755 ± 0.067	0.829AlaHis: 0.829 ± 0.029	5.107AlaIle: 5.107 ± 0.078	4.151AlaLys: 4.151 ± 0.077	5.844AlaLeu: 5.844 ± 0.082	1.482AlaMet: 1.482 ± 0.042	2.371AlaAsn: 2.371 ± 0.051	1.422AlaPro: 1.422 ± 0.036	1.598AlaGln: 1.598 ± 0.042	2.056AlaArg: 2.056 ± 0.042	3.578AlaSer: 3.578 ± 0.064	2.43AlaThr: 2.43 ± 0.088	4.667AlaVal: 4.667 ± 0.083	0.354AlaTrp: 0.354 ± 0.016	2.114AlaTyr: 2.114 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.712CysAla: 0.712 ± 0.027	0.24CysCys: 0.24 ± 0.014	0.721CysAsp: 0.721 ± 0.025	0.881CysGlu: 0.881 ± 0.026	0.556CysPhe: 0.556 ± 0.022	1.158CysGly: 1.158 ± 0.036	0.258CysHis: 0.258 ± 0.016	1.317CysIle: 1.317 ± 0.038	1.042CysLys: 1.042 ± 0.033	0.926CysLeu: 0.926 ± 0.03	0.347CysMet: 0.347 ± 0.016	0.784CysAsn: 0.784 ± 0.03	0.496CysPro: 0.496 ± 0.024	0.268CysGln: 0.268 ± 0.017	0.583CysArg: 0.583 ± 0.023	0.957CysSer: 0.957 ± 0.039	0.627CysThr: 0.627 ± 0.027	0.741CysVal: 0.741 ± 0.026	0.082CysTrp: 0.082 ± 0.008	0.555CysTyr: 0.555 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	2.634AspAla: 2.634 ± 0.057	0.718AspCys: 0.718 ± 0.027	2.394AspAsp: 2.394 ± 0.053	4.017AspGlu: 4.017 ± 0.071	2.662AspPhe: 2.662 ± 0.048	3.066AspGly: 3.066 ± 0.06	0.525AspHis: 0.525 ± 0.024	6.409AspIle: 6.409 ± 0.084	5.408AspLys: 5.408 ± 0.072	4.448AspLeu: 4.448 ± 0.061	1.669AspMet: 1.669 ± 0.039	3.288AspAsn: 3.288 ± 0.064	1.296AspPro: 1.296 ± 0.038	0.74AspGln: 0.74 ± 0.029	2.002AspArg: 2.002 ± 0.045	3.284AspSer: 3.284 ± 0.061	2.775AspThr: 2.775 ± 0.065	3.428AspVal: 3.428 ± 0.057	0.387AspTrp: 0.387 ± 0.019	2.341AspTyr: 2.341 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	3.804GluAla: 3.804 ± 0.058	0.712GluCys: 0.712 ± 0.026	4.337GluAsp: 4.337 ± 0.075	6.057GluGlu: 6.057 ± 0.098	2.873GluPhe: 2.873 ± 0.056	3.889GluGly: 3.889 ± 0.062	1.003GluHis: 1.003 ± 0.031	6.582GluIle: 6.582 ± 0.094	7.267GluLys: 7.267 ± 0.096	6.417GluLeu: 6.417 ± 0.088	1.775GluMet: 1.775 ± 0.035	5.31GluAsn: 5.31 ± 0.079	1.378GluPro: 1.378 ± 0.035	2.16GluGln: 2.16 ± 0.049	2.518GluArg: 2.518 ± 0.053	3.634GluSer: 3.634 ± 0.059	2.692GluThr: 2.692 ± 0.055	4.34GluVal: 4.34 ± 0.068	0.403GluTrp: 0.403 ± 0.019	2.9GluTyr: 2.9 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	2.114PheAla: 2.114 ± 0.048	0.577PheCys: 0.577 ± 0.024	2.233PheAsp: 2.233 ± 0.044	2.496PheGlu: 2.496 ± 0.049	1.886PhePhe: 1.886 ± 0.051	2.703PheGly: 2.703 ± 0.053	0.673PheHis: 0.673 ± 0.025	4.641PheIle: 4.641 ± 0.078	3.919PheLys: 3.919 ± 0.065	3.842PheLeu: 3.842 ± 0.07	1.197PheMet: 1.197 ± 0.033	2.845PheAsn: 2.845 ± 0.051	1.272PhePro: 1.272 ± 0.037	1.252PheGln: 1.252 ± 0.031	1.323PheArg: 1.323 ± 0.034	3.282PheSer: 3.282 ± 0.06	2.449PheThr: 2.449 ± 0.048	2.437PheVal: 2.437 ± 0.052	0.321PheTrp: 0.321 ± 0.017	1.861PheTyr: 1.861 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	3.877GlyAla: 3.877 ± 0.103	1.008GlyCys: 1.008 ± 0.034	3.238GlyAsp: 3.238 ± 0.057	4.198GlyGlu: 4.198 ± 0.074	2.916GlyPhe: 2.916 ± 0.048	4.318GlyGly: 4.318 ± 0.085	1.026GlyHis: 1.026 ± 0.036	7.036GlyIle: 7.036 ± 0.091	5.627GlyLys: 5.627 ± 0.078	4.97GlyLeu: 4.97 ± 0.069	1.755GlyMet: 1.755 ± 0.038	3.388GlyAsn: 3.388 ± 0.053	1.09GlyPro: 1.09 ± 0.044	1.496GlyGln: 1.496 ± 0.04	2.439GlyArg: 2.439 ± 0.053	3.857GlySer: 3.857 ± 0.067	3.576GlyThr: 3.576 ± 0.064	4.273GlyVal: 4.273 ± 0.063	0.498GlyTrp: 0.498 ± 0.024	2.933GlyTyr: 2.933 ± 0.055	0.0GlyXaa: 0.0 ± 0.0
His	0.7HisAla: 0.7 ± 0.028	0.26HisCys: 0.26 ± 0.015	0.651HisAsp: 0.651 ± 0.022	0.873HisGlu: 0.873 ± 0.03	0.652HisPhe: 0.652 ± 0.026	0.995HisGly: 0.995 ± 0.031	0.327HisHis: 0.327 ± 0.02	1.531HisIle: 1.531 ± 0.04	1.174HisLys: 1.174 ± 0.032	1.153HisLeu: 1.153 ± 0.034	0.429HisMet: 0.429 ± 0.02	0.845HisAsn: 0.845 ± 0.029	0.654HisPro: 0.654 ± 0.027	0.369HisGln: 0.369 ± 0.02	0.654HisArg: 0.654 ± 0.026	0.989HisSer: 0.989 ± 0.035	0.695HisThr: 0.695 ± 0.025	0.833HisVal: 0.833 ± 0.026	0.124HisTrp: 0.124 ± 0.01	0.592HisTyr: 0.592 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.605IleAla: 5.605 ± 0.081	1.43IleCys: 1.43 ± 0.043	5.491IleAsp: 5.491 ± 0.074	6.758IleGlu: 6.758 ± 0.085	4.412IlePhe: 4.412 ± 0.077	6.159IleGly: 6.159 ± 0.086	1.309IleHis: 1.309 ± 0.034	9.66IleIle: 9.66 ± 0.134	8.842IleLys: 8.842 ± 0.109	9.2IleLeu: 9.2 ± 0.112	2.525IleMet: 2.525 ± 0.054	6.051IleAsn: 6.051 ± 0.082	3.605IlePro: 3.605 ± 0.059	2.567IleGln: 2.567 ± 0.048	3.309IleArg: 3.309 ± 0.053	7.369IleSer: 7.369 ± 0.087	5.085IleThr: 5.085 ± 0.072	6.097IleVal: 6.097 ± 0.084	0.62IleTrp: 0.62 ± 0.024	3.789IleTyr: 3.789 ± 0.065	0.0IleXaa: 0.0 ± 0.0
Lys	4.621LysAla: 4.621 ± 0.076	0.988LysCys: 0.988 ± 0.031	5.536LysAsp: 5.536 ± 0.079	7.466LysGlu: 7.466 ± 0.096	3.347LysPhe: 3.347 ± 0.054	5.037LysGly: 5.037 ± 0.071	1.179LysHis: 1.179 ± 0.03	8.738LysIle: 8.738 ± 0.087	8.307LysLys: 8.307 ± 0.095	7.72LysLeu: 7.72 ± 0.076	2.444LysMet: 2.444 ± 0.049	6.901LysAsn: 6.901 ± 0.099	2.147LysPro: 2.147 ± 0.048	2.402LysGln: 2.402 ± 0.051	3.176LysArg: 3.176 ± 0.055	5.521LysSer: 5.521 ± 0.074	3.826LysThr: 3.826 ± 0.059	5.743LysVal: 5.743 ± 0.08	0.697LysTrp: 0.697 ± 0.027	4.234LysTyr: 4.234 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	4.837LeuAla: 4.837 ± 0.062	1.273LeuCys: 1.273 ± 0.035	4.756LeuAsp: 4.756 ± 0.069	5.913LeuGlu: 5.913 ± 0.092	3.641LeuPhe: 3.641 ± 0.067	5.748LeuGly: 5.748 ± 0.085	1.219LeuHis: 1.219 ± 0.034	7.989LeuIle: 7.989 ± 0.108	8.947LeuLys: 8.947 ± 0.087	7.483LeuLeu: 7.483 ± 0.114	2.279LeuMet: 2.279 ± 0.044	6.03LeuAsn: 6.03 ± 0.075	2.833LeuPro: 2.833 ± 0.056	2.398LeuGln: 2.398 ± 0.049	3.174LeuArg: 3.174 ± 0.05	6.593LeuSer: 6.593 ± 0.08	4.325LeuThr: 4.325 ± 0.084	4.969LeuVal: 4.969 ± 0.069	0.664LeuTrp: 0.664 ± 0.027	3.294LeuTyr: 3.294 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	1.848MetAla: 1.848 ± 0.048	0.319MetCys: 0.319 ± 0.016	1.781MetAsp: 1.781 ± 0.043	2.116MetGlu: 2.116 ± 0.046	0.99MetPhe: 0.99 ± 0.032	1.89MetGly: 1.89 ± 0.04	0.356MetHis: 0.356 ± 0.017	2.167MetIle: 2.167 ± 0.047	2.458MetLys: 2.458 ± 0.048	2.336MetLeu: 2.336 ± 0.048	0.63MetMet: 0.63 ± 0.028	1.686MetAsn: 1.686 ± 0.038	0.946MetPro: 0.946 ± 0.027	0.638MetGln: 0.638 ± 0.025	0.944MetArg: 0.944 ± 0.031	1.765MetSer: 1.765 ± 0.037	1.23MetThr: 1.23 ± 0.029	1.683MetVal: 1.683 ± 0.044	0.171MetTrp: 0.171 ± 0.012	0.899MetTyr: 0.899 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	2.889AsnAla: 2.889 ± 0.055	0.843AsnCys: 0.843 ± 0.031	2.623AsnAsp: 2.623 ± 0.054	3.715AsnGlu: 3.715 ± 0.065	2.719AsnPhe: 2.719 ± 0.049	3.414AsnGly: 3.414 ± 0.069	0.843AsnHis: 0.843 ± 0.027	7.413AsnIle: 7.413 ± 0.088	5.926AsnLys: 5.926 ± 0.086	5.599AsnLeu: 5.599 ± 0.075	1.839AsnMet: 1.839 ± 0.048	4.055AsnAsn: 4.055 ± 0.08	2.064AsnPro: 2.064 ± 0.044	1.476AsnGln: 1.476 ± 0.044	2.247AsnArg: 2.247 ± 0.052	4.308AsnSer: 4.308 ± 0.078	3.269AsnThr: 3.269 ± 0.072	3.706AsnVal: 3.706 ± 0.056	0.485AsnTrp: 0.485 ± 0.021	2.758AsnTyr: 2.758 ± 0.053	0.0AsnXaa: 0.0 ± 0.0
Pro	1.46ProAla: 1.46 ± 0.04	0.375ProCys: 0.375 ± 0.02	1.618ProAsp: 1.618 ± 0.037	2.475ProGlu: 2.475 ± 0.048	1.345ProPhe: 1.345 ± 0.039	1.765ProGly: 1.765 ± 0.036	0.508ProHis: 0.508 ± 0.025	2.737ProIle: 2.737 ± 0.048	2.247ProLys: 2.247 ± 0.047	2.614ProLeu: 2.614 ± 0.043	0.721ProMet: 0.721 ± 0.025	1.362ProAsn: 1.362 ± 0.032	0.759ProPro: 0.759 ± 0.032	0.866ProGln: 0.866 ± 0.031	0.872ProArg: 0.872 ± 0.031	1.82ProSer: 1.82 ± 0.039	1.403ProThr: 1.403 ± 0.045	2.239ProVal: 2.239 ± 0.046	0.246ProTrp: 0.246 ± 0.013	1.416ProTyr: 1.416 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	1.495GlnAla: 1.495 ± 0.045	0.348GlnCys: 0.348 ± 0.017	1.306GlnAsp: 1.306 ± 0.031	1.711GlnGlu: 1.711 ± 0.042	1.085GlnPhe: 1.085 ± 0.031	1.732GlnGly: 1.732 ± 0.041	0.39GlnHis: 0.39 ± 0.018	2.516GlnIle: 2.516 ± 0.053	2.326GlnLys: 2.326 ± 0.04	2.306GlnLeu: 2.306 ± 0.054	0.768GlnMet: 0.768 ± 0.028	1.703GlnAsn: 1.703 ± 0.041	0.64GlnPro: 0.64 ± 0.026	0.96GlnGln: 0.96 ± 0.036	1.017GlnArg: 1.017 ± 0.03	1.639GlnSer: 1.639 ± 0.038	1.192GlnThr: 1.192 ± 0.038	1.663GlnVal: 1.663 ± 0.039	0.242GlnTrp: 0.242 ± 0.015	1.131GlnTyr: 1.131 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	1.996ArgAla: 1.996 ± 0.042	0.448ArgCys: 0.448 ± 0.021	1.895ArgAsp: 1.895 ± 0.041	3.208ArgGlu: 3.208 ± 0.059	1.483ArgPhe: 1.483 ± 0.037	2.154ArgGly: 2.154 ± 0.04	0.56ArgHis: 0.56 ± 0.022	3.412ArgIle: 3.412 ± 0.054	3.336ArgLys: 3.336 ± 0.058	3.116ArgLeu: 3.116 ± 0.058	1.0ArgMet: 1.0 ± 0.034	2.106ArgAsn: 2.106 ± 0.046	0.966ArgPro: 0.966 ± 0.028	1.079ArgGln: 1.079 ± 0.031	1.631ArgArg: 1.631 ± 0.047	1.75ArgSer: 1.75 ± 0.041	1.691ArgThr: 1.691 ± 0.036	2.152ArgVal: 2.152 ± 0.046	0.319ArgTrp: 0.319 ± 0.019	1.47ArgTyr: 1.47 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	3.509SerAla: 3.509 ± 0.065	0.782SerCys: 0.782 ± 0.028	3.184SerAsp: 3.184 ± 0.059	4.178SerGlu: 4.178 ± 0.07	2.948SerPhe: 2.948 ± 0.056	4.773SerGly: 4.773 ± 0.076	1.031SerHis: 1.031 ± 0.032	7.077SerIle: 7.077 ± 0.089	5.617SerLys: 5.617 ± 0.071	5.748SerLeu: 5.748 ± 0.071	1.844SerMet: 1.844 ± 0.04	3.82SerAsn: 3.82 ± 0.073	1.866SerPro: 1.866 ± 0.04	1.941SerGln: 1.941 ± 0.045	2.31SerArg: 2.31 ± 0.045	4.906SerSer: 4.906 ± 0.081	3.393SerThr: 3.393 ± 0.06	4.145SerVal: 4.145 ± 0.067	0.463SerTrp: 0.463 ± 0.02	2.759SerTyr: 2.759 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	3.312ThrAla: 3.312 ± 0.065	0.565ThrCys: 0.565 ± 0.023	2.502ThrAsp: 2.502 ± 0.046	2.981ThrGlu: 2.981 ± 0.055	2.118ThrPhe: 2.118 ± 0.046	3.862ThrGly: 3.862 ± 0.15	0.775ThrHis: 0.775 ± 0.026	4.712ThrIle: 4.712 ± 0.073	3.473ThrLys: 3.473 ± 0.06	4.803ThrLeu: 4.803 ± 0.074	1.107ThrMet: 1.107 ± 0.032	2.496ThrAsn: 2.496 ± 0.056	1.874ThrPro: 1.874 ± 0.046	1.243ThrGln: 1.243 ± 0.038	1.539ThrArg: 1.539 ± 0.033	3.411ThrSer: 3.411 ± 0.063	2.627ThrThr: 2.627 ± 0.065	3.548ThrVal: 3.548 ± 0.066	0.384ThrTrp: 0.384 ± 0.019	1.803ThrTyr: 1.803 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	3.401ValAla: 3.401 ± 0.062	0.942ValCys: 0.942 ± 0.031	3.678ValAsp: 3.678 ± 0.064	4.404ValGlu: 4.404 ± 0.074	2.859ValPhe: 2.859 ± 0.052	3.883ValGly: 3.883 ± 0.073	0.94ValHis: 0.94 ± 0.031	6.035ValIle: 6.035 ± 0.079	5.686ValLys: 5.686 ± 0.082	5.727ValLeu: 5.727 ± 0.072	1.67ValMet: 1.67 ± 0.034	3.756ValAsn: 3.756 ± 0.06	2.032ValPro: 2.032 ± 0.044	1.623ValGln: 1.623 ± 0.035	2.052ValArg: 2.052 ± 0.043	4.468ValSer: 4.468 ± 0.081	3.425ValThr: 3.425 ± 0.066	4.481ValVal: 4.481 ± 0.073	0.397ValTrp: 0.397 ± 0.019	2.48ValTyr: 2.48 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.347TrpAla: 0.347 ± 0.018	0.113TrpCys: 0.113 ± 0.011	0.407TrpAsp: 0.407 ± 0.019	0.46TrpGlu: 0.46 ± 0.023	0.335TrpPhe: 0.335 ± 0.016	0.538TrpGly: 0.538 ± 0.023	0.153TrpHis: 0.153 ± 0.012	0.731TrpIle: 0.731 ± 0.027	0.584TrpLys: 0.584 ± 0.024	0.613TrpLeu: 0.613 ± 0.024	0.209TrpMet: 0.209 ± 0.016	0.499TrpAsn: 0.499 ± 0.021	0.173TrpPro: 0.173 ± 0.013	0.264TrpGln: 0.264 ± 0.018	0.301TrpArg: 0.301 ± 0.017	0.412TrpSer: 0.412 ± 0.02	0.356TrpThr: 0.356 ± 0.02	0.419TrpVal: 0.419 ± 0.019	0.089TrpTrp: 0.089 ± 0.009	0.255TrpTyr: 0.255 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.938TyrAla: 1.938 ± 0.04	0.607TyrCys: 0.607 ± 0.025	2.392TyrAsp: 2.392 ± 0.045	2.733TyrGlu: 2.733 ± 0.053	2.019TyrPhe: 2.019 ± 0.047	2.681TyrGly: 2.681 ± 0.048	0.597TyrHis: 0.597 ± 0.023	4.07TyrIle: 4.07 ± 0.066	3.692TyrLys: 3.692 ± 0.056	3.501TyrLeu: 3.501 ± 0.061	1.161TyrMet: 1.161 ± 0.033	2.897TyrAsn: 2.897 ± 0.06	1.332TyrPro: 1.332 ± 0.034	0.828TyrGln: 0.828 ± 0.027	1.624TyrArg: 1.624 ± 0.036	2.751TyrSer: 2.751 ± 0.054	2.073TyrThr: 2.073 ± 0.045	2.374TyrVal: 2.374 ± 0.044	0.309TyrTrp: 0.309 ± 0.017	1.88TyrTyr: 1.88 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3828 proteins (1110703 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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