Streptococcus phage Javan269

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AAB1|A0A4D6AAB1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan269 OX=2548084 GN=Javan269_0021 PE=4 SV=1
MM1 pKa = 6.57WTRR4 pKa = 11.84GQIHH8 pKa = 5.65YY9 pKa = 10.31HH10 pKa = 4.96EE11 pKa = 5.07QIVDD15 pKa = 3.87YY16 pKa = 9.86IAKK19 pKa = 10.32VSDD22 pKa = 3.4QPSDD26 pKa = 3.33VGIDD30 pKa = 3.56LGCVFKK36 pKa = 11.58LEE38 pKa = 3.98VDD40 pKa = 3.65VAEE43 pKa = 4.3NTIISYY49 pKa = 10.75DD50 pKa = 4.09RR51 pKa = 11.84GWDD54 pKa = 3.22IYY56 pKa = 10.71PEE58 pKa = 4.19SDD60 pKa = 3.25EE61 pKa = 4.15QEE63 pKa = 4.8AILEE67 pKa = 4.56VILKK71 pKa = 6.83TLKK74 pKa = 10.34VV75 pKa = 3.44

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A6L4|A0A4D6A6L4_9CAUD DNA helicase OS=Streptococcus phage Javan269 OX=2548084 GN=Javan269_0009 PE=4 SV=1
MM1 pKa = 7.42IFSIFMTILMAVWLFTGLYY20 pKa = 8.95AAFMTIRR27 pKa = 11.84DD28 pKa = 4.57DD29 pKa = 3.38IRR31 pKa = 11.84SEE33 pKa = 4.06KK34 pKa = 10.07VRR36 pKa = 11.84KK37 pKa = 9.15QKK39 pKa = 9.08WW40 pKa = 3.02

Molecular weight: 4.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

10661

40

1046

273.4

30.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.125 ± 0.387

0.797 ± 0.181

6.013 ± 0.321

7.345 ± 0.343

3.846 ± 0.21

6.5 ± 0.305

1.745 ± 0.165

7.232 ± 0.378

7.982 ± 0.265

9.286 ± 0.323

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.354 ± 0.202

5.318 ± 0.276

2.805 ± 0.228

4.043 ± 0.205

4.39 ± 0.288

6.81 ± 0.44

6.444 ± 0.454

5.909 ± 0.23

1.454 ± 0.153

3.602 ± 0.3
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski