Amino acid dipepetide frequency for Streptococcus phage Javan269

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.096AlaAla: 3.096 ± 0.653
0.375AlaCys: 0.375 ± 0.183
3.752AlaAsp: 3.752 ± 0.502
4.409AlaGlu: 4.409 ± 0.672
2.251AlaPhe: 2.251 ± 0.32
3.94AlaGly: 3.94 ± 0.588
0.75AlaHis: 0.75 ± 0.22
4.69AlaIle: 4.69 ± 0.985
5.441AlaLys: 5.441 ± 0.595
5.816AlaLeu: 5.816 ± 0.862
1.501AlaMet: 1.501 ± 0.379
3.377AlaAsn: 3.377 ± 0.5
1.22AlaPro: 1.22 ± 0.454
2.064AlaGln: 2.064 ± 0.454
3.002AlaArg: 3.002 ± 0.556
4.128AlaSer: 4.128 ± 0.841
3.659AlaThr: 3.659 ± 0.632
3.659AlaVal: 3.659 ± 0.533
0.469AlaTrp: 0.469 ± 0.281
3.659AlaTyr: 3.659 ± 0.532
0.0AlaXaa: 0.0 ± 0.0
Cys
0.75CysAla: 0.75 ± 0.388
0.188CysCys: 0.188 ± 0.131
0.469CysAsp: 0.469 ± 0.191
0.563CysGlu: 0.563 ± 0.281
0.281CysPhe: 0.281 ± 0.155
0.563CysGly: 0.563 ± 0.235
0.469CysHis: 0.469 ± 0.169
0.469CysIle: 0.469 ± 0.211
0.281CysLys: 0.281 ± 0.167
0.75CysLeu: 0.75 ± 0.277
0.0CysMet: 0.0 ± 0.0
0.281CysAsn: 0.281 ± 0.142
0.281CysPro: 0.281 ± 0.147
0.657CysGln: 0.657 ± 0.27
0.375CysArg: 0.375 ± 0.183
0.281CysSer: 0.281 ± 0.147
0.281CysThr: 0.281 ± 0.14
0.375CysVal: 0.375 ± 0.195
0.0CysTrp: 0.0 ± 0.0
0.657CysTyr: 0.657 ± 0.25
0.0CysXaa: 0.0 ± 0.0
Asp
3.096AspAla: 3.096 ± 0.524
0.281AspCys: 0.281 ± 0.173
3.94AspAsp: 3.94 ± 0.885
5.816AspGlu: 5.816 ± 0.885
3.752AspPhe: 3.752 ± 0.631
3.189AspGly: 3.189 ± 0.748
0.657AspHis: 0.657 ± 0.254
5.253AspIle: 5.253 ± 0.564
5.159AspLys: 5.159 ± 0.586
5.535AspLeu: 5.535 ± 0.859
1.501AspMet: 1.501 ± 0.282
2.627AspAsn: 2.627 ± 0.495
2.064AspPro: 2.064 ± 0.507
1.22AspGln: 1.22 ± 0.344
2.533AspArg: 2.533 ± 0.504
4.409AspSer: 4.409 ± 0.657
2.72AspThr: 2.72 ± 0.48
3.189AspVal: 3.189 ± 0.479
0.657AspTrp: 0.657 ± 0.25
2.533AspTyr: 2.533 ± 0.733
0.0AspXaa: 0.0 ± 0.0
Glu
4.128GluAla: 4.128 ± 0.591
0.844GluCys: 0.844 ± 0.287
3.659GluAsp: 3.659 ± 0.691
5.441GluGlu: 5.441 ± 1.032
2.345GluPhe: 2.345 ± 0.551
5.159GluGly: 5.159 ± 0.594
1.595GluHis: 1.595 ± 0.44
4.503GluIle: 4.503 ± 0.535
6.191GluLys: 6.191 ± 0.681
8.443GluLeu: 8.443 ± 0.8
2.627GluMet: 2.627 ± 0.572
4.503GluAsn: 4.503 ± 0.619
1.876GluPro: 1.876 ± 0.359
4.034GluGln: 4.034 ± 0.516
2.251GluArg: 2.251 ± 0.5
4.409GluSer: 4.409 ± 0.662
4.784GluThr: 4.784 ± 0.528
4.128GluVal: 4.128 ± 0.627
0.844GluTrp: 0.844 ± 0.303
1.689GluTyr: 1.689 ± 0.425
0.0GluXaa: 0.0 ± 0.0
Phe
2.251PheAla: 2.251 ± 0.361
0.657PheCys: 0.657 ± 0.242
2.533PheAsp: 2.533 ± 0.479
2.533PheGlu: 2.533 ± 0.577
1.595PhePhe: 1.595 ± 0.408
2.345PheGly: 2.345 ± 0.373
1.032PheHis: 1.032 ± 0.276
2.814PheIle: 2.814 ± 0.561
2.908PheLys: 2.908 ± 0.646
3.096PheLeu: 3.096 ± 0.554
1.501PheMet: 1.501 ± 0.385
2.251PheAsn: 2.251 ± 0.448
0.657PhePro: 0.657 ± 0.23
1.689PheGln: 1.689 ± 0.4
1.689PheArg: 1.689 ± 0.319
2.627PheSer: 2.627 ± 0.481
2.064PheThr: 2.064 ± 0.559
1.782PheVal: 1.782 ± 0.396
0.563PheTrp: 0.563 ± 0.194
1.876PheTyr: 1.876 ± 0.466
0.0PheXaa: 0.0 ± 0.0
Gly
3.94GlyAla: 3.94 ± 0.576
0.469GlyCys: 0.469 ± 0.225
4.034GlyAsp: 4.034 ± 0.524
3.565GlyGlu: 3.565 ± 0.468
2.72GlyPhe: 2.72 ± 0.507
4.221GlyGly: 4.221 ± 0.607
1.313GlyHis: 1.313 ± 0.359
5.441GlyIle: 5.441 ± 0.777
4.972GlyLys: 4.972 ± 0.519
6.66GlyLeu: 6.66 ± 0.693
2.251GlyMet: 2.251 ± 0.44
3.94GlyAsn: 3.94 ± 0.725
0.375GlyPro: 0.375 ± 0.151
2.251GlyGln: 2.251 ± 0.483
3.471GlyArg: 3.471 ± 0.557
3.752GlySer: 3.752 ± 0.75
4.128GlyThr: 4.128 ± 0.836
3.846GlyVal: 3.846 ± 0.682
1.22GlyTrp: 1.22 ± 0.495
2.439GlyTyr: 2.439 ± 0.457
0.0GlyXaa: 0.0 ± 0.0
His
0.657HisAla: 0.657 ± 0.21
0.188HisCys: 0.188 ± 0.199
1.032HisAsp: 1.032 ± 0.309
1.313HisGlu: 1.313 ± 0.393
0.657HisPhe: 0.657 ± 0.235
1.22HisGly: 1.22 ± 0.336
0.281HisHis: 0.281 ± 0.159
1.501HisIle: 1.501 ± 0.323
0.938HisLys: 0.938 ± 0.309
2.251HisLeu: 2.251 ± 0.482
0.469HisMet: 0.469 ± 0.212
0.844HisAsn: 0.844 ± 0.273
0.75HisPro: 0.75 ± 0.357
0.563HisGln: 0.563 ± 0.206
0.938HisArg: 0.938 ± 0.307
0.844HisSer: 0.844 ± 0.289
1.032HisThr: 1.032 ± 0.364
0.938HisVal: 0.938 ± 0.304
0.188HisTrp: 0.188 ± 0.124
0.844HisTyr: 0.844 ± 0.364
0.0HisXaa: 0.0 ± 0.0
Ile
4.878IleAla: 4.878 ± 0.68
0.844IleCys: 0.844 ± 0.259
5.066IleAsp: 5.066 ± 0.562
6.285IleGlu: 6.285 ± 0.775
1.97IlePhe: 1.97 ± 0.37
5.066IleGly: 5.066 ± 0.674
1.126IleHis: 1.126 ± 0.382
4.034IleIle: 4.034 ± 0.755
6.379IleLys: 6.379 ± 0.844
6.379IleLeu: 6.379 ± 0.696
1.22IleMet: 1.22 ± 0.247
3.565IleAsn: 3.565 ± 0.872
1.876IlePro: 1.876 ± 0.377
2.158IleGln: 2.158 ± 0.552
2.533IleArg: 2.533 ± 0.527
6.66IleSer: 6.66 ± 1.284
4.597IleThr: 4.597 ± 0.888
4.69IleVal: 4.69 ± 0.701
1.126IleTrp: 1.126 ± 0.285
1.876IleTyr: 1.876 ± 0.294
0.0IleXaa: 0.0 ± 0.0
Lys
6.098LysAla: 6.098 ± 0.612
0.563LysCys: 0.563 ± 0.246
5.253LysAsp: 5.253 ± 0.707
6.848LysGlu: 6.848 ± 0.877
2.533LysPhe: 2.533 ± 0.406
4.221LysGly: 4.221 ± 0.513
1.22LysHis: 1.22 ± 0.358
6.004LysIle: 6.004 ± 0.923
5.159LysLys: 5.159 ± 0.798
7.223LysLeu: 7.223 ± 0.796
1.22LysMet: 1.22 ± 0.396
3.752LysAsn: 3.752 ± 0.549
2.814LysPro: 2.814 ± 0.471
4.409LysGln: 4.409 ± 0.806
4.128LysArg: 4.128 ± 0.629
6.004LysSer: 6.004 ± 0.896
5.253LysThr: 5.253 ± 0.793
3.94LysVal: 3.94 ± 0.695
1.407LysTrp: 1.407 ± 0.458
1.782LysTyr: 1.782 ± 0.449
0.0LysXaa: 0.0 ± 0.0
Leu
7.317LeuAla: 7.317 ± 0.928
0.375LeuCys: 0.375 ± 0.204
5.159LeuAsp: 5.159 ± 0.534
7.317LeuGlu: 7.317 ± 0.908
2.72LeuPhe: 2.72 ± 0.538
4.69LeuGly: 4.69 ± 0.595
1.126LeuHis: 1.126 ± 0.345
5.441LeuIle: 5.441 ± 0.755
8.724LeuLys: 8.724 ± 0.887
7.223LeuLeu: 7.223 ± 0.983
2.439LeuMet: 2.439 ± 0.484
4.69LeuAsn: 4.69 ± 0.502
3.283LeuPro: 3.283 ± 0.53
3.752LeuGln: 3.752 ± 0.505
4.221LeuArg: 4.221 ± 0.584
6.942LeuSer: 6.942 ± 0.936
6.66LeuThr: 6.66 ± 0.69
5.722LeuVal: 5.722 ± 0.772
0.844LeuTrp: 0.844 ± 0.208
4.221LeuTyr: 4.221 ± 0.981
0.0LeuXaa: 0.0 ± 0.0
Met
1.407MetAla: 1.407 ± 0.32
0.094MetCys: 0.094 ± 0.099
2.251MetAsp: 2.251 ± 0.437
1.876MetGlu: 1.876 ± 0.437
0.75MetPhe: 0.75 ± 0.245
2.064MetGly: 2.064 ± 0.428
0.094MetHis: 0.094 ± 0.094
1.876MetIle: 1.876 ± 0.452
1.595MetLys: 1.595 ± 0.429
1.876MetLeu: 1.876 ± 0.422
0.75MetMet: 0.75 ± 0.277
0.938MetAsn: 0.938 ± 0.355
0.281MetPro: 0.281 ± 0.136
0.844MetGln: 0.844 ± 0.23
1.313MetArg: 1.313 ± 0.339
1.407MetSer: 1.407 ± 0.511
2.345MetThr: 2.345 ± 0.518
1.313MetVal: 1.313 ± 0.476
0.281MetTrp: 0.281 ± 0.14
0.188MetTyr: 0.188 ± 0.137
0.0MetXaa: 0.0 ± 0.0
Asn
3.94AsnAla: 3.94 ± 0.851
0.094AsnCys: 0.094 ± 0.097
2.158AsnAsp: 2.158 ± 0.459
4.034AsnGlu: 4.034 ± 0.53
2.251AsnPhe: 2.251 ± 0.47
5.629AsnGly: 5.629 ± 0.629
1.501AsnHis: 1.501 ± 0.397
2.72AsnIle: 2.72 ± 0.48
2.72AsnLys: 2.72 ± 0.421
5.253AsnLeu: 5.253 ± 0.861
1.22AsnMet: 1.22 ± 0.374
1.876AsnAsn: 1.876 ± 0.474
2.064AsnPro: 2.064 ± 0.419
2.251AsnGln: 2.251 ± 0.34
2.814AsnArg: 2.814 ± 0.435
4.034AsnSer: 4.034 ± 0.88
3.096AsnThr: 3.096 ± 0.775
3.189AsnVal: 3.189 ± 0.438
0.844AsnTrp: 0.844 ± 0.23
1.501AsnTyr: 1.501 ± 0.4
0.0AsnXaa: 0.0 ± 0.0
Pro
0.844ProAla: 0.844 ± 0.256
0.188ProCys: 0.188 ± 0.118
1.501ProAsp: 1.501 ± 0.251
1.501ProGlu: 1.501 ± 0.451
1.032ProPhe: 1.032 ± 0.283
1.032ProGly: 1.032 ± 0.461
0.563ProHis: 0.563 ± 0.271
2.439ProIle: 2.439 ± 0.459
2.627ProLys: 2.627 ± 0.544
2.533ProLeu: 2.533 ± 0.515
0.469ProMet: 0.469 ± 0.187
2.064ProAsn: 2.064 ± 0.452
0.938ProPro: 0.938 ± 0.344
0.938ProGln: 0.938 ± 0.376
1.501ProArg: 1.501 ± 0.344
2.064ProSer: 2.064 ± 0.466
2.345ProThr: 2.345 ± 0.67
1.595ProVal: 1.595 ± 0.355
0.469ProTrp: 0.469 ± 0.254
1.407ProTyr: 1.407 ± 0.432
0.0ProXaa: 0.0 ± 0.0
Gln
3.189GlnAla: 3.189 ± 0.86
0.094GlnCys: 0.094 ± 0.082
1.032GlnAsp: 1.032 ± 0.239
3.471GlnGlu: 3.471 ± 0.522
2.533GlnPhe: 2.533 ± 0.535
1.876GlnGly: 1.876 ± 0.458
1.032GlnHis: 1.032 ± 0.293
3.096GlnIle: 3.096 ± 0.669
3.002GlnLys: 3.002 ± 0.554
3.096GlnLeu: 3.096 ± 0.78
1.313GlnMet: 1.313 ± 0.387
2.814GlnAsn: 2.814 ± 0.512
1.782GlnPro: 1.782 ± 0.536
1.313GlnGln: 1.313 ± 0.387
1.407GlnArg: 1.407 ± 0.322
1.97GlnSer: 1.97 ± 0.395
2.533GlnThr: 2.533 ± 0.522
3.189GlnVal: 3.189 ± 0.506
0.657GlnTrp: 0.657 ± 0.288
1.032GlnTyr: 1.032 ± 0.368
0.0GlnXaa: 0.0 ± 0.0
Arg
1.407ArgAla: 1.407 ± 0.3
0.657ArgCys: 0.657 ± 0.243
2.72ArgAsp: 2.72 ± 0.628
3.752ArgGlu: 3.752 ± 0.565
1.501ArgPhe: 1.501 ± 0.396
2.627ArgGly: 2.627 ± 0.424
0.75ArgHis: 0.75 ± 0.279
3.659ArgIle: 3.659 ± 0.509
4.503ArgLys: 4.503 ± 1.034
3.752ArgLeu: 3.752 ± 0.453
1.032ArgMet: 1.032 ± 0.286
2.72ArgAsn: 2.72 ± 0.405
1.126ArgPro: 1.126 ± 0.384
2.439ArgGln: 2.439 ± 0.461
1.501ArgArg: 1.501 ± 0.413
1.782ArgSer: 1.782 ± 0.454
2.627ArgThr: 2.627 ± 0.596
3.002ArgVal: 3.002 ± 0.59
0.657ArgTrp: 0.657 ± 0.267
1.689ArgTyr: 1.689 ± 0.5
0.0ArgXaa: 0.0 ± 0.0
Ser
3.096SerAla: 3.096 ± 0.725
0.375SerCys: 0.375 ± 0.197
4.597SerAsp: 4.597 ± 0.636
4.315SerGlu: 4.315 ± 0.855
2.627SerPhe: 2.627 ± 0.567
5.535SerGly: 5.535 ± 0.853
1.313SerHis: 1.313 ± 0.301
4.972SerIle: 4.972 ± 0.726
4.972SerLys: 4.972 ± 0.698
5.816SerLeu: 5.816 ± 0.56
1.032SerMet: 1.032 ± 0.298
3.752SerAsn: 3.752 ± 0.584
2.251SerPro: 2.251 ± 0.488
2.533SerGln: 2.533 ± 0.772
3.189SerArg: 3.189 ± 0.591
4.597SerSer: 4.597 ± 0.985
5.253SerThr: 5.253 ± 1.154
3.752SerVal: 3.752 ± 0.777
1.126SerTrp: 1.126 ± 0.273
3.002SerTyr: 3.002 ± 0.505
0.0SerXaa: 0.0 ± 0.0
Thr
4.784ThrAla: 4.784 ± 0.689
0.188ThrCys: 0.188 ± 0.155
3.283ThrAsp: 3.283 ± 0.526
2.814ThrGlu: 2.814 ± 0.476
2.814ThrPhe: 2.814 ± 0.726
5.066ThrGly: 5.066 ± 0.903
0.657ThrHis: 0.657 ± 0.203
5.347ThrIle: 5.347 ± 1.13
5.629ThrLys: 5.629 ± 0.67
5.816ThrLeu: 5.816 ± 0.545
0.844ThrMet: 0.844 ± 0.22
4.128ThrAsn: 4.128 ± 0.805
1.595ThrPro: 1.595 ± 0.357
2.064ThrGln: 2.064 ± 0.481
2.064ThrArg: 2.064 ± 0.417
4.69ThrSer: 4.69 ± 0.757
4.784ThrThr: 4.784 ± 0.842
5.253ThrVal: 5.253 ± 0.79
1.032ThrTrp: 1.032 ± 0.263
1.595ThrTyr: 1.595 ± 0.353
0.0ThrXaa: 0.0 ± 0.0
Val
3.096ValAla: 3.096 ± 0.578
0.281ValCys: 0.281 ± 0.208
4.315ValAsp: 4.315 ± 0.694
3.659ValGlu: 3.659 ± 0.644
1.97ValPhe: 1.97 ± 0.452
3.283ValGly: 3.283 ± 0.647
0.938ValHis: 0.938 ± 0.329
4.878ValIle: 4.878 ± 0.815
4.597ValLys: 4.597 ± 0.649
7.411ValLeu: 7.411 ± 0.668
1.126ValMet: 1.126 ± 0.299
2.064ValAsn: 2.064 ± 0.322
1.501ValPro: 1.501 ± 0.335
2.439ValGln: 2.439 ± 0.638
2.439ValArg: 2.439 ± 0.455
4.128ValSer: 4.128 ± 0.642
3.846ValThr: 3.846 ± 0.487
3.659ValVal: 3.659 ± 0.538
1.313ValTrp: 1.313 ± 0.317
2.064ValTyr: 2.064 ± 0.444
0.0ValXaa: 0.0 ± 0.0
Trp
0.75TrpAla: 0.75 ± 0.309
0.375TrpCys: 0.375 ± 0.178
0.75TrpAsp: 0.75 ± 0.28
1.126TrpGlu: 1.126 ± 0.427
0.938TrpPhe: 0.938 ± 0.369
0.563TrpGly: 0.563 ± 0.16
0.0TrpHis: 0.0 ± 0.0
0.844TrpIle: 0.844 ± 0.296
1.126TrpLys: 1.126 ± 0.394
1.22TrpLeu: 1.22 ± 0.266
0.563TrpMet: 0.563 ± 0.209
1.501TrpAsn: 1.501 ± 0.427
0.094TrpPro: 0.094 ± 0.088
0.844TrpGln: 0.844 ± 0.271
0.657TrpArg: 0.657 ± 0.253
0.938TrpSer: 0.938 ± 0.289
0.75TrpThr: 0.75 ± 0.207
0.657TrpVal: 0.657 ± 0.23
0.375TrpTrp: 0.375 ± 0.215
0.469TrpTyr: 0.469 ± 0.307
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.533TyrAla: 2.533 ± 0.541
0.844TyrCys: 0.844 ± 0.329
2.627TyrAsp: 2.627 ± 0.614
2.814TyrGlu: 2.814 ± 0.408
1.22TyrPhe: 1.22 ± 0.381
2.72TyrGly: 2.72 ± 0.43
1.032TyrHis: 1.032 ± 0.337
2.345TyrIle: 2.345 ± 0.527
2.908TyrLys: 2.908 ± 0.561
2.72TyrLeu: 2.72 ± 0.581
0.375TyrMet: 0.375 ± 0.194
1.501TyrAsn: 1.501 ± 0.324
1.22TyrPro: 1.22 ± 0.372
1.97TyrGln: 1.97 ± 0.463
1.876TyrArg: 1.876 ± 0.397
2.439TyrSer: 2.439 ± 0.372
1.689TyrThr: 1.689 ± 0.462
1.22TyrVal: 1.22 ± 0.292
0.469TyrTrp: 0.469 ± 0.197
1.501TyrTyr: 1.501 ± 0.503
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 39 proteins (10661 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski