Streptomyces phage Evy

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Samistivirus; unclassified Samistivirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DK36|A0A514DK36_9CAUD Uncharacterized protein OS=Streptomyces phage Evy OX=2588514 GN=138 PE=4 SV=1
MM1 pKa = 7.93AYY3 pKa = 10.52YY4 pKa = 9.88PINQFFYY11 pKa = 10.93DD12 pKa = 3.93SEE14 pKa = 4.61TNNIIVQFAVYY25 pKa = 8.87TEE27 pKa = 4.23KK28 pKa = 10.31TYY30 pKa = 9.62EE31 pKa = 4.11TANMDD36 pKa = 3.52DD37 pKa = 4.4PYY39 pKa = 11.73DD40 pKa = 4.01VIEE43 pKa = 5.37AEE45 pKa = 4.47LFLDD49 pKa = 3.7AGKK52 pKa = 10.55FKK54 pKa = 10.5IGEE57 pKa = 4.03YY58 pKa = 10.27RR59 pKa = 11.84PASLDD64 pKa = 3.26GTITQEE70 pKa = 4.62FIGRR74 pKa = 11.84DD75 pKa = 3.39LGQSPNITPPNPVPEE90 pKa = 4.46EE91 pKa = 3.88PAA93 pKa = 4.07

Molecular weight: 10.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DK08|A0A514DK08_9CAUD Metallophosphoesterase OS=Streptomyces phage Evy OX=2588514 GN=70 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.24KK3 pKa = 10.29KK4 pKa = 10.62KK5 pKa = 8.78LTLYY9 pKa = 10.64KK10 pKa = 10.41SEE12 pKa = 3.5TWLRR16 pKa = 11.84AKK18 pKa = 10.24YY19 pKa = 8.81RR20 pKa = 11.84TMNAAEE26 pKa = 4.15IATFCGVTEE35 pKa = 4.1MTITRR40 pKa = 11.84YY41 pKa = 9.69LDD43 pKa = 2.97KK44 pKa = 11.07FNIVRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84SRR53 pKa = 3.08

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

204

0

204

34583

37

2095

169.5

19.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.484 ± 0.323

0.948 ± 0.115

6.584 ± 0.198

6.891 ± 0.358

3.846 ± 0.131

7.469 ± 0.206

1.911 ± 0.129

5.373 ± 0.127

6.266 ± 0.312

7.174 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.822 ± 0.112

4.537 ± 0.159

3.745 ± 0.165

3.296 ± 0.185

5.572 ± 0.194

6.087 ± 0.299

6.113 ± 0.353

7.269 ± 0.211

1.908 ± 0.098

3.704 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski