Amino acid dipepetide frequency for Streptomyces phage Evy

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.328AlaAla: 8.328 ± 1.001
0.781AlaCys: 0.781 ± 0.171
5.089AlaAsp: 5.089 ± 0.444
6.073AlaGlu: 6.073 ± 0.612
3.181AlaPhe: 3.181 ± 0.287
5.61AlaGly: 5.61 ± 0.475
1.504AlaHis: 1.504 ± 0.181
4.656AlaIle: 4.656 ± 0.481
5.35AlaLys: 5.35 ± 0.483
7.114AlaLeu: 7.114 ± 0.527
2.429AlaMet: 2.429 ± 0.31
3.875AlaAsn: 3.875 ± 0.427
3.21AlaPro: 3.21 ± 0.44
2.892AlaGln: 2.892 ± 0.31
4.338AlaArg: 4.338 ± 0.423
4.742AlaSer: 4.742 ± 0.572
5.176AlaThr: 5.176 ± 0.5
5.783AlaVal: 5.783 ± 0.379
1.475AlaTrp: 1.475 ± 0.216
3.239AlaTyr: 3.239 ± 0.353
0.0AlaXaa: 0.0 ± 0.0
Cys
0.405CysAla: 0.405 ± 0.113
0.202CysCys: 0.202 ± 0.103
0.665CysAsp: 0.665 ± 0.191
0.607CysGlu: 0.607 ± 0.132
0.231CysPhe: 0.231 ± 0.091
0.896CysGly: 0.896 ± 0.209
0.26CysHis: 0.26 ± 0.098
0.665CysIle: 0.665 ± 0.153
0.723CysLys: 0.723 ± 0.141
0.636CysLeu: 0.636 ± 0.132
0.289CysMet: 0.289 ± 0.103
0.434CysAsn: 0.434 ± 0.131
0.521CysPro: 0.521 ± 0.134
0.318CysGln: 0.318 ± 0.112
0.636CysArg: 0.636 ± 0.178
0.694CysSer: 0.694 ± 0.153
0.289CysThr: 0.289 ± 0.118
0.521CysVal: 0.521 ± 0.118
0.145CysTrp: 0.145 ± 0.067
0.347CysTyr: 0.347 ± 0.109
0.0CysXaa: 0.0 ± 0.0
Asp
5.754AspAla: 5.754 ± 0.471
0.434AspCys: 0.434 ± 0.122
4.28AspAsp: 4.28 ± 0.459
5.581AspGlu: 5.581 ± 0.462
3.181AspPhe: 3.181 ± 0.315
5.957AspGly: 5.957 ± 0.566
0.896AspHis: 0.896 ± 0.156
3.759AspIle: 3.759 ± 0.368
4.251AspLys: 4.251 ± 0.397
4.569AspLeu: 4.569 ± 0.399
1.937AspMet: 1.937 ± 0.226
2.545AspAsn: 2.545 ± 0.254
2.313AspPro: 2.313 ± 0.274
1.504AspGln: 1.504 ± 0.215
2.978AspArg: 2.978 ± 0.281
4.048AspSer: 4.048 ± 0.363
3.499AspThr: 3.499 ± 0.413
4.019AspVal: 4.019 ± 0.492
1.677AspTrp: 1.677 ± 0.232
2.66AspTyr: 2.66 ± 0.302
0.0AspXaa: 0.0 ± 0.0
Glu
5.986GluAla: 5.986 ± 0.599
0.405GluCys: 0.405 ± 0.122
4.077GluAsp: 4.077 ± 0.351
4.887GluGlu: 4.887 ± 0.548
2.574GluPhe: 2.574 ± 0.265
4.193GluGly: 4.193 ± 0.438
1.648GluHis: 1.648 ± 0.256
4.395GluIle: 4.395 ± 0.357
4.366GluLys: 4.366 ± 0.475
5.263GluLeu: 5.263 ± 0.475
2.024GluMet: 2.024 ± 0.249
3.528GluAsn: 3.528 ± 0.321
2.082GluPro: 2.082 ± 0.332
2.921GluGln: 2.921 ± 0.334
4.28GluArg: 4.28 ± 0.446
3.297GluSer: 3.297 ± 0.39
3.759GluThr: 3.759 ± 0.375
5.292GluVal: 5.292 ± 0.502
1.475GluTrp: 1.475 ± 0.217
2.458GluTyr: 2.458 ± 0.323
0.0GluXaa: 0.0 ± 0.0
Phe
2.66PheAla: 2.66 ± 0.286
0.492PheCys: 0.492 ± 0.118
3.644PheAsp: 3.644 ± 0.263
3.383PheGlu: 3.383 ± 0.31
1.822PhePhe: 1.822 ± 0.225
2.892PheGly: 2.892 ± 0.32
0.839PheHis: 0.839 ± 0.184
1.937PheIle: 1.937 ± 0.24
2.14PheLys: 2.14 ± 0.255
2.111PheLeu: 2.111 ± 0.333
0.81PheMet: 0.81 ± 0.149
1.937PheAsn: 1.937 ± 0.231
0.925PhePro: 0.925 ± 0.15
1.099PheGln: 1.099 ± 0.205
1.735PheArg: 1.735 ± 0.241
2.776PheSer: 2.776 ± 0.343
2.574PheThr: 2.574 ± 0.285
2.834PheVal: 2.834 ± 0.307
0.578PheTrp: 0.578 ± 0.12
1.272PheTyr: 1.272 ± 0.185
0.0PheXaa: 0.0 ± 0.0
Gly
5.176GlyAla: 5.176 ± 0.479
0.694GlyCys: 0.694 ± 0.143
4.309GlyAsp: 4.309 ± 0.438
4.424GlyGlu: 4.424 ± 0.431
3.297GlyPhe: 3.297 ± 0.292
5.118GlyGly: 5.118 ± 0.446
1.735GlyHis: 1.735 ± 0.241
4.135GlyIle: 4.135 ± 0.366
4.713GlyLys: 4.713 ± 0.42
5.754GlyLeu: 5.754 ± 0.446
2.342GlyMet: 2.342 ± 0.241
3.846GlyAsn: 3.846 ± 0.289
2.082GlyPro: 2.082 ± 0.291
2.631GlyGln: 2.631 ± 0.242
4.135GlyArg: 4.135 ± 0.362
4.395GlySer: 4.395 ± 0.582
5.147GlyThr: 5.147 ± 0.586
6.246GlyVal: 6.246 ± 0.422
1.562GlyTrp: 1.562 ± 0.228
2.95GlyTyr: 2.95 ± 0.226
0.0GlyXaa: 0.0 ± 0.0
His
1.157HisAla: 1.157 ± 0.202
0.347HisCys: 0.347 ± 0.091
1.33HisAsp: 1.33 ± 0.188
0.896HisGlu: 0.896 ± 0.188
0.752HisPhe: 0.752 ± 0.147
1.417HisGly: 1.417 ± 0.198
0.463HisHis: 0.463 ± 0.122
1.243HisIle: 1.243 ± 0.216
1.041HisLys: 1.041 ± 0.16
1.533HisLeu: 1.533 ± 0.226
0.578HisMet: 0.578 ± 0.142
0.752HisAsn: 0.752 ± 0.135
0.781HisPro: 0.781 ± 0.159
0.492HisGln: 0.492 ± 0.134
1.533HisArg: 1.533 ± 0.248
0.983HisSer: 0.983 ± 0.174
0.983HisThr: 0.983 ± 0.164
1.475HisVal: 1.475 ± 0.243
0.347HisTrp: 0.347 ± 0.101
1.012HisTyr: 1.012 ± 0.159
0.0HisXaa: 0.0 ± 0.0
Ile
5.118IleAla: 5.118 ± 0.407
0.636IleCys: 0.636 ± 0.157
4.309IleAsp: 4.309 ± 0.357
4.685IleGlu: 4.685 ± 0.376
1.33IlePhe: 1.33 ± 0.209
3.586IleGly: 3.586 ± 0.339
0.983IleHis: 0.983 ± 0.172
2.053IleIle: 2.053 ± 0.256
3.325IleLys: 3.325 ± 0.413
3.412IleLeu: 3.412 ± 0.436
1.099IleMet: 1.099 ± 0.184
2.342IleAsn: 2.342 ± 0.332
2.313IlePro: 2.313 ± 0.279
2.053IleGln: 2.053 ± 0.323
3.47IleArg: 3.47 ± 0.371
2.95IleSer: 2.95 ± 0.269
2.834IleThr: 2.834 ± 0.332
4.656IleVal: 4.656 ± 0.404
0.896IleTrp: 0.896 ± 0.166
1.677IleTyr: 1.677 ± 0.26
0.0IleXaa: 0.0 ± 0.0
Lys
6.101LysAla: 6.101 ± 0.434
0.549LysCys: 0.549 ± 0.144
3.586LysAsp: 3.586 ± 0.396
3.528LysGlu: 3.528 ± 0.44
2.284LysPhe: 2.284 ± 0.297
4.338LysGly: 4.338 ± 0.383
0.954LysHis: 0.954 ± 0.185
2.834LysIle: 2.834 ± 0.284
4.106LysLys: 4.106 ± 0.533
4.28LysLeu: 4.28 ± 0.363
2.284LysMet: 2.284 ± 0.229
3.268LysAsn: 3.268 ± 0.312
2.487LysPro: 2.487 ± 0.29
2.371LysGln: 2.371 ± 0.258
3.759LysArg: 3.759 ± 0.414
3.557LysSer: 3.557 ± 0.409
3.817LysThr: 3.817 ± 0.362
4.713LysVal: 4.713 ± 0.4
1.243LysTrp: 1.243 ± 0.185
2.603LysTyr: 2.603 ± 0.248
0.0LysXaa: 0.0 ± 0.0
Leu
7.432LeuAla: 7.432 ± 0.51
0.723LeuCys: 0.723 ± 0.204
5.176LeuAsp: 5.176 ± 0.436
5.234LeuGlu: 5.234 ± 0.378
2.429LeuPhe: 2.429 ± 0.276
4.916LeuGly: 4.916 ± 0.412
1.504LeuHis: 1.504 ± 0.215
4.048LeuIle: 4.048 ± 0.342
4.28LeuLys: 4.28 ± 0.458
4.569LeuLeu: 4.569 ± 0.41
2.082LeuMet: 2.082 ± 0.307
3.239LeuAsn: 3.239 ± 0.322
2.95LeuPro: 2.95 ± 0.272
1.822LeuGln: 1.822 ± 0.266
3.875LeuArg: 3.875 ± 0.348
4.829LeuSer: 4.829 ± 0.366
4.598LeuThr: 4.598 ± 0.396
4.366LeuVal: 4.366 ± 0.41
1.417LeuTrp: 1.417 ± 0.186
2.256LeuTyr: 2.256 ± 0.286
0.0LeuXaa: 0.0 ± 0.0
Met
2.834MetAla: 2.834 ± 0.313
0.26MetCys: 0.26 ± 0.095
1.475MetAsp: 1.475 ± 0.236
1.533MetGlu: 1.533 ± 0.242
0.781MetPhe: 0.781 ± 0.161
2.169MetGly: 2.169 ± 0.392
0.578MetHis: 0.578 ± 0.134
1.157MetIle: 1.157 ± 0.184
2.053MetLys: 2.053 ± 0.223
1.851MetLeu: 1.851 ± 0.24
0.405MetMet: 0.405 ± 0.138
1.243MetAsn: 1.243 ± 0.19
1.417MetPro: 1.417 ± 0.227
1.099MetGln: 1.099 ± 0.219
2.024MetArg: 2.024 ± 0.289
2.429MetSer: 2.429 ± 0.266
2.169MetThr: 2.169 ± 0.274
1.59MetVal: 1.59 ± 0.217
0.318MetTrp: 0.318 ± 0.098
0.839MetTyr: 0.839 ± 0.161
0.0MetXaa: 0.0 ± 0.0
Asn
3.788AsnAla: 3.788 ± 0.348
0.405AsnCys: 0.405 ± 0.111
3.268AsnAsp: 3.268 ± 0.279
2.718AsnGlu: 2.718 ± 0.31
1.59AsnPhe: 1.59 ± 0.185
4.453AsnGly: 4.453 ± 0.373
0.983AsnHis: 0.983 ± 0.158
2.863AsnIle: 2.863 ± 0.254
2.978AsnLys: 2.978 ± 0.399
3.412AsnLeu: 3.412 ± 0.334
1.359AsnMet: 1.359 ± 0.199
2.111AsnAsn: 2.111 ± 0.314
1.793AsnPro: 1.793 ± 0.239
1.186AsnGln: 1.186 ± 0.2
2.256AsnArg: 2.256 ± 0.284
2.284AsnSer: 2.284 ± 0.307
2.545AsnThr: 2.545 ± 0.375
2.978AsnVal: 2.978 ± 0.354
0.81AsnTrp: 0.81 ± 0.148
1.562AsnTyr: 1.562 ± 0.224
0.0AsnXaa: 0.0 ± 0.0
Pro
2.921ProAla: 2.921 ± 0.322
0.289ProCys: 0.289 ± 0.099
2.747ProAsp: 2.747 ± 0.328
2.718ProGlu: 2.718 ± 0.283
1.619ProPhe: 1.619 ± 0.208
3.354ProGly: 3.354 ± 0.374
0.607ProHis: 0.607 ± 0.132
1.793ProIle: 1.793 ± 0.218
2.458ProLys: 2.458 ± 0.345
2.198ProLeu: 2.198 ± 0.243
0.839ProMet: 0.839 ± 0.165
1.735ProAsn: 1.735 ± 0.259
1.243ProPro: 1.243 ± 0.247
0.839ProGln: 0.839 ± 0.162
1.966ProArg: 1.966 ± 0.241
2.169ProSer: 2.169 ± 0.318
2.66ProThr: 2.66 ± 0.37
3.701ProVal: 3.701 ± 0.338
0.405ProTrp: 0.405 ± 0.111
1.186ProTyr: 1.186 ± 0.204
0.0ProXaa: 0.0 ± 0.0
Gln
3.036GlnAla: 3.036 ± 0.494
0.405GlnCys: 0.405 ± 0.115
1.706GlnAsp: 1.706 ± 0.225
2.284GlnGlu: 2.284 ± 0.27
1.562GlnPhe: 1.562 ± 0.204
2.227GlnGly: 2.227 ± 0.238
0.492GlnHis: 0.492 ± 0.122
1.388GlnIle: 1.388 ± 0.195
2.284GlnLys: 2.284 ± 0.273
2.747GlnLeu: 2.747 ± 0.347
1.359GlnMet: 1.359 ± 0.186
1.33GlnAsn: 1.33 ± 0.234
1.07GlnPro: 1.07 ± 0.189
1.07GlnGln: 1.07 ± 0.327
2.082GlnArg: 2.082 ± 0.276
2.14GlnSer: 2.14 ± 0.224
1.909GlnThr: 1.909 ± 0.239
2.371GlnVal: 2.371 ± 0.276
0.405GlnTrp: 0.405 ± 0.1
1.099GlnTyr: 1.099 ± 0.154
0.0GlnXaa: 0.0 ± 0.0
Arg
4.627ArgAla: 4.627 ± 0.488
0.434ArgCys: 0.434 ± 0.127
3.499ArgAsp: 3.499 ± 0.388
3.904ArgGlu: 3.904 ± 0.418
2.458ArgPhe: 2.458 ± 0.313
3.672ArgGly: 3.672 ± 0.29
0.925ArgHis: 0.925 ± 0.188
3.297ArgIle: 3.297 ± 0.335
3.846ArgLys: 3.846 ± 0.5
3.904ArgLeu: 3.904 ± 0.327
1.706ArgMet: 1.706 ± 0.24
2.545ArgAsn: 2.545 ± 0.263
1.88ArgPro: 1.88 ± 0.232
2.024ArgGln: 2.024 ± 0.276
3.47ArgArg: 3.47 ± 0.357
2.921ArgSer: 2.921 ± 0.306
2.978ArgThr: 2.978 ± 0.304
4.048ArgVal: 4.048 ± 0.299
1.186ArgTrp: 1.186 ± 0.206
2.4ArgTyr: 2.4 ± 0.27
0.0ArgXaa: 0.0 ± 0.0
Ser
4.713SerAla: 4.713 ± 0.49
0.723SerCys: 0.723 ± 0.158
3.875SerAsp: 3.875 ± 0.293
3.615SerGlu: 3.615 ± 0.329
2.516SerPhe: 2.516 ± 0.278
5.552SerGly: 5.552 ± 0.611
1.157SerHis: 1.157 ± 0.189
3.615SerIle: 3.615 ± 0.396
3.615SerLys: 3.615 ± 0.36
4.395SerLeu: 4.395 ± 0.342
1.793SerMet: 1.793 ± 0.187
2.603SerAsn: 2.603 ± 0.325
2.053SerPro: 2.053 ± 0.267
2.082SerGln: 2.082 ± 0.253
3.268SerArg: 3.268 ± 0.342
3.759SerSer: 3.759 ± 0.412
3.759SerThr: 3.759 ± 0.706
4.54SerVal: 4.54 ± 0.468
1.215SerTrp: 1.215 ± 0.178
2.024SerTyr: 2.024 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
4.685ThrAla: 4.685 ± 0.465
0.549ThrCys: 0.549 ± 0.121
4.135ThrAsp: 4.135 ± 0.448
4.135ThrGlu: 4.135 ± 0.39
2.14ThrPhe: 2.14 ± 0.267
5.292ThrGly: 5.292 ± 0.57
1.041ThrHis: 1.041 ± 0.188
3.557ThrIle: 3.557 ± 0.385
3.441ThrLys: 3.441 ± 0.331
4.309ThrLeu: 4.309 ± 0.366
1.388ThrMet: 1.388 ± 0.2
2.66ThrAsn: 2.66 ± 0.369
2.921ThrPro: 2.921 ± 0.373
1.909ThrGln: 1.909 ± 0.275
2.776ThrArg: 2.776 ± 0.318
3.846ThrSer: 3.846 ± 0.397
4.019ThrThr: 4.019 ± 0.761
4.771ThrVal: 4.771 ± 0.496
1.388ThrTrp: 1.388 ± 0.209
2.169ThrTyr: 2.169 ± 0.258
0.0ThrXaa: 0.0 ± 0.0
Val
5.639ValAla: 5.639 ± 0.436
0.665ValCys: 0.665 ± 0.143
4.974ValAsp: 4.974 ± 0.471
4.685ValGlu: 4.685 ± 0.458
2.776ValPhe: 2.776 ± 0.303
4.453ValGly: 4.453 ± 0.36
1.417ValHis: 1.417 ± 0.245
3.817ValIle: 3.817 ± 0.349
4.858ValLys: 4.858 ± 0.465
4.771ValLeu: 4.771 ± 0.41
1.851ValMet: 1.851 ± 0.256
2.95ValAsn: 2.95 ± 0.381
3.354ValPro: 3.354 ± 0.316
2.487ValGln: 2.487 ± 0.324
4.222ValArg: 4.222 ± 0.368
5.263ValSer: 5.263 ± 0.425
4.54ValThr: 4.54 ± 0.499
5.494ValVal: 5.494 ± 0.415
1.359ValTrp: 1.359 ± 0.269
3.123ValTyr: 3.123 ± 0.355
0.0ValXaa: 0.0 ± 0.0
Trp
1.562TrpAla: 1.562 ± 0.215
0.174TrpCys: 0.174 ± 0.067
1.243TrpAsp: 1.243 ± 0.218
1.59TrpGlu: 1.59 ± 0.223
0.781TrpPhe: 0.781 ± 0.129
1.388TrpGly: 1.388 ± 0.208
0.492TrpHis: 0.492 ± 0.114
0.81TrpIle: 0.81 ± 0.178
0.983TrpLys: 0.983 ± 0.194
1.764TrpLeu: 1.764 ± 0.279
0.636TrpMet: 0.636 ± 0.15
0.983TrpAsn: 0.983 ± 0.186
0.434TrpPro: 0.434 ± 0.117
0.752TrpGln: 0.752 ± 0.149
0.781TrpArg: 0.781 ± 0.153
1.157TrpSer: 1.157 ± 0.179
1.272TrpThr: 1.272 ± 0.186
0.983TrpVal: 0.983 ± 0.191
0.492TrpTrp: 0.492 ± 0.105
0.81TrpTyr: 0.81 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.921TyrAla: 2.921 ± 0.319
0.318TyrCys: 0.318 ± 0.104
2.458TyrAsp: 2.458 ± 0.356
2.429TyrGlu: 2.429 ± 0.329
1.157TyrPhe: 1.157 ± 0.187
3.21TyrGly: 3.21 ± 0.327
0.636TyrHis: 0.636 ± 0.138
1.706TyrIle: 1.706 ± 0.23
1.851TyrLys: 1.851 ± 0.266
3.152TyrLeu: 3.152 ± 0.334
0.983TyrMet: 0.983 ± 0.186
1.446TyrAsn: 1.446 ± 0.219
1.619TyrPro: 1.619 ± 0.249
1.417TyrGln: 1.417 ± 0.194
2.14TyrArg: 2.14 ± 0.262
2.631TyrSer: 2.631 ± 0.291
2.603TyrThr: 2.603 ± 0.273
2.313TyrVal: 2.313 ± 0.262
0.694TyrTrp: 0.694 ± 0.128
1.359TyrTyr: 1.359 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 204 proteins (34583 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski