Streptococcus phage Javan590

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BFM6|A0A4D6BFM6_9CAUD Site-specific recombinase OS=Streptococcus phage Javan590 OX=2548278 GN=Javan590_0025 PE=3 SV=1
MM1 pKa = 7.83VLEE4 pKa = 4.22VHH6 pKa = 6.95DD7 pKa = 4.64FEE9 pKa = 6.45YY10 pKa = 11.2DD11 pKa = 3.27NQVYY15 pKa = 10.51VDD17 pKa = 4.28FEE19 pKa = 4.3GVRR22 pKa = 11.84YY23 pKa = 9.13EE24 pKa = 4.4VIKK27 pKa = 10.07TFEE30 pKa = 4.21NGDD33 pKa = 4.0IIEE36 pKa = 4.36LTCEE40 pKa = 4.01VVKK43 pKa = 11.15NGTGSNN49 pKa = 3.57

Molecular weight: 5.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BEF6|A0A4D6BEF6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan590 OX=2548278 GN=Javan590_0034 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84IVKK5 pKa = 9.92FEE7 pKa = 4.01KK8 pKa = 9.36TSVWKK13 pKa = 9.3TNNIKK18 pKa = 10.32QFEE21 pKa = 4.47KK22 pKa = 10.67KK23 pKa = 10.07RR24 pKa = 11.84NLTQYY29 pKa = 9.56VLKK32 pKa = 10.53IIQRR36 pKa = 11.84EE37 pKa = 3.91IGIVFAPKK45 pKa = 9.61ILLSRR50 pKa = 11.84VVV52 pKa = 3.05

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

9947

38

891

191.3

21.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.057 ± 0.491

0.543 ± 0.127

5.962 ± 0.276

7.721 ± 0.412

4.454 ± 0.291

5.881 ± 0.5

1.759 ± 0.233

7.299 ± 0.364

8.002 ± 0.388

8.455 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.604 ± 0.217

5.771 ± 0.25

2.915 ± 0.161

4.353 ± 0.243

4.444 ± 0.411

5.761 ± 0.317

5.861 ± 0.38

6.293 ± 0.339

1.216 ± 0.111

3.649 ± 0.365
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski