Amino acid dipepetide frequency for Polaribacter phage P12002L

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.373AlaAla: 1.373 ± 0.57
0.412AlaCys: 0.412 ± 0.217
3.02AlaAsp: 3.02 ± 0.47
3.501AlaGlu: 3.501 ± 0.482
2.059AlaPhe: 2.059 ± 0.376
1.716AlaGly: 1.716 ± 0.472
0.549AlaHis: 0.549 ± 0.239
3.295AlaIle: 3.295 ± 0.522
5.08AlaLys: 5.08 ± 0.501
4.256AlaLeu: 4.256 ± 0.699
0.892AlaMet: 0.892 ± 0.229
3.089AlaAsn: 3.089 ± 0.479
0.824AlaPro: 0.824 ± 0.198
1.236AlaGln: 1.236 ± 0.397
1.922AlaArg: 1.922 ± 0.345
3.638AlaSer: 3.638 ± 0.848
2.883AlaThr: 2.883 ± 0.557
2.059AlaVal: 2.059 ± 0.387
0.412AlaTrp: 0.412 ± 0.183
1.647AlaTyr: 1.647 ± 0.374
0.0AlaXaa: 0.0 ± 0.0
Cys
0.275CysAla: 0.275 ± 0.145
0.069CysCys: 0.069 ± 0.073
0.961CysAsp: 0.961 ± 0.264
0.549CysGlu: 0.549 ± 0.244
0.481CysPhe: 0.481 ± 0.189
1.098CysGly: 1.098 ± 0.323
0.0CysHis: 0.0 ± 0.0
0.824CysIle: 0.824 ± 0.24
0.824CysLys: 0.824 ± 0.322
0.961CysLeu: 0.961 ± 0.308
0.0CysMet: 0.0 ± 0.0
0.686CysAsn: 0.686 ± 0.225
0.069CysPro: 0.069 ± 0.065
0.137CysGln: 0.137 ± 0.09
0.412CysArg: 0.412 ± 0.171
0.618CysSer: 0.618 ± 0.213
0.618CysThr: 0.618 ± 0.174
0.481CysVal: 0.481 ± 0.18
0.206CysTrp: 0.206 ± 0.118
0.137CysTyr: 0.137 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
2.746AspAla: 2.746 ± 0.591
0.686AspCys: 0.686 ± 0.251
2.952AspAsp: 2.952 ± 0.388
3.844AspGlu: 3.844 ± 0.521
3.364AspPhe: 3.364 ± 0.466
3.913AspGly: 3.913 ± 0.667
0.481AspHis: 0.481 ± 0.166
5.972AspIle: 5.972 ± 0.502
6.727AspLys: 6.727 ± 0.578
6.796AspLeu: 6.796 ± 0.593
1.442AspMet: 1.442 ± 0.25
4.805AspAsn: 4.805 ± 0.539
1.098AspPro: 1.098 ± 0.297
0.961AspGln: 0.961 ± 0.242
1.579AspArg: 1.579 ± 0.306
3.364AspSer: 3.364 ± 0.567
2.677AspThr: 2.677 ± 0.392
3.226AspVal: 3.226 ± 0.437
1.03AspTrp: 1.03 ± 0.319
2.471AspTyr: 2.471 ± 0.54
0.0AspXaa: 0.0 ± 0.0
Glu
2.952GluAla: 2.952 ± 0.544
0.412GluCys: 0.412 ± 0.188
3.226GluAsp: 3.226 ± 0.386
5.835GluGlu: 5.835 ± 0.782
3.638GluPhe: 3.638 ± 0.453
3.364GluGly: 3.364 ± 0.431
0.892GluHis: 0.892 ± 0.276
5.972GluIle: 5.972 ± 0.705
8.306GluLys: 8.306 ± 0.969
9.061GluLeu: 9.061 ± 0.967
1.373GluMet: 1.373 ± 0.322
5.629GluAsn: 5.629 ± 0.583
1.922GluPro: 1.922 ± 0.342
3.02GluGln: 3.02 ± 0.377
3.981GluArg: 3.981 ± 0.642
3.775GluSer: 3.775 ± 0.751
3.844GluThr: 3.844 ± 0.585
4.668GluVal: 4.668 ± 0.545
0.824GluTrp: 0.824 ± 0.214
3.707GluTyr: 3.707 ± 0.433
0.0GluXaa: 0.0 ± 0.0
Phe
1.304PheAla: 1.304 ± 0.3
0.549PheCys: 0.549 ± 0.226
3.158PheAsp: 3.158 ± 0.367
2.814PheGlu: 2.814 ± 0.483
1.579PhePhe: 1.579 ± 0.414
2.883PheGly: 2.883 ± 0.333
0.412PheHis: 0.412 ± 0.161
3.02PheIle: 3.02 ± 0.538
4.805PheLys: 4.805 ± 0.66
4.05PheLeu: 4.05 ± 0.479
1.236PheMet: 1.236 ± 0.406
3.775PheAsn: 3.775 ± 0.54
0.412PhePro: 0.412 ± 0.202
1.098PheGln: 1.098 ± 0.226
1.853PheArg: 1.853 ± 0.335
3.295PheSer: 3.295 ± 0.437
3.089PheThr: 3.089 ± 0.552
2.608PheVal: 2.608 ± 0.365
0.686PheTrp: 0.686 ± 0.289
1.373PheTyr: 1.373 ± 0.274
0.0PheXaa: 0.0 ± 0.0
Gly
2.403GlyAla: 2.403 ± 0.467
0.686GlyCys: 0.686 ± 0.249
3.02GlyAsp: 3.02 ± 0.509
3.432GlyGlu: 3.432 ± 0.434
2.814GlyPhe: 2.814 ± 0.469
3.844GlyGly: 3.844 ± 0.767
0.549GlyHis: 0.549 ± 0.256
3.844GlyIle: 3.844 ± 0.413
5.08GlyLys: 5.08 ± 0.497
5.217GlyLeu: 5.217 ± 0.468
0.961GlyMet: 0.961 ± 0.27
4.187GlyAsn: 4.187 ± 0.808
0.0GlyPro: 0.0 ± 0.0
1.03GlyGln: 1.03 ± 0.26
1.922GlyArg: 1.922 ± 0.307
4.187GlySer: 4.187 ± 0.496
3.226GlyThr: 3.226 ± 0.529
4.668GlyVal: 4.668 ± 0.571
0.686GlyTrp: 0.686 ± 0.231
3.501GlyTyr: 3.501 ± 0.63
0.0GlyXaa: 0.0 ± 0.0
His
0.343HisAla: 0.343 ± 0.168
0.0HisCys: 0.0 ± 0.0
0.618HisAsp: 0.618 ± 0.241
1.098HisGlu: 1.098 ± 0.304
0.549HisPhe: 0.549 ± 0.191
0.755HisGly: 0.755 ± 0.262
0.0HisHis: 0.0 ± 0.0
1.098HisIle: 1.098 ± 0.305
1.647HisLys: 1.647 ± 0.365
0.824HisLeu: 0.824 ± 0.231
0.069HisMet: 0.069 ± 0.071
0.824HisAsn: 0.824 ± 0.267
0.618HisPro: 0.618 ± 0.247
0.618HisGln: 0.618 ± 0.267
0.618HisArg: 0.618 ± 0.213
1.098HisSer: 1.098 ± 0.292
0.618HisThr: 0.618 ± 0.193
1.098HisVal: 1.098 ± 0.298
0.069HisTrp: 0.069 ± 0.062
0.549HisTyr: 0.549 ± 0.229
0.0HisXaa: 0.0 ± 0.0
Ile
3.638IleAla: 3.638 ± 0.561
0.549IleCys: 0.549 ± 0.258
5.972IleAsp: 5.972 ± 0.495
6.315IleGlu: 6.315 ± 0.737
2.677IlePhe: 2.677 ± 0.336
4.256IleGly: 4.256 ± 0.65
0.961IleHis: 0.961 ± 0.273
5.217IleIle: 5.217 ± 0.725
10.297IleLys: 10.297 ± 0.947
5.972IleLeu: 5.972 ± 0.756
1.373IleMet: 1.373 ± 0.319
6.658IleAsn: 6.658 ± 0.796
2.197IlePro: 2.197 ± 0.376
2.471IleGln: 2.471 ± 0.535
2.334IleArg: 2.334 ± 0.424
6.521IleSer: 6.521 ± 0.8
4.256IleThr: 4.256 ± 0.609
3.432IleVal: 3.432 ± 0.381
1.236IleTrp: 1.236 ± 0.247
2.608IleTyr: 2.608 ± 0.42
0.0IleXaa: 0.0 ± 0.0
Lys
5.629LysAla: 5.629 ± 0.888
1.098LysCys: 1.098 ± 0.308
6.59LysAsp: 6.59 ± 0.826
11.326LysGlu: 11.326 ± 1.148
3.569LysPhe: 3.569 ± 0.529
5.629LysGly: 5.629 ± 0.679
1.51LysHis: 1.51 ± 0.345
7.894LysIle: 7.894 ± 0.798
12.974LysLys: 12.974 ± 1.531
8.786LysLeu: 8.786 ± 0.675
2.471LysMet: 2.471 ± 0.499
7.688LysAsn: 7.688 ± 1.023
3.364LysPro: 3.364 ± 0.681
3.775LysGln: 3.775 ± 0.634
3.638LysArg: 3.638 ± 0.431
5.835LysSer: 5.835 ± 0.644
6.796LysThr: 6.796 ± 0.58
5.835LysVal: 5.835 ± 0.495
0.961LysTrp: 0.961 ± 0.287
5.972LysTyr: 5.972 ± 0.868
0.0LysXaa: 0.0 ± 0.0
Leu
4.53LeuAla: 4.53 ± 0.642
0.618LeuCys: 0.618 ± 0.208
5.629LeuAsp: 5.629 ± 0.536
6.933LeuGlu: 6.933 ± 0.771
3.295LeuPhe: 3.295 ± 0.373
5.423LeuGly: 5.423 ± 0.742
1.716LeuHis: 1.716 ± 0.315
7.208LeuIle: 7.208 ± 0.653
10.502LeuLys: 10.502 ± 1.157
6.041LeuLeu: 6.041 ± 0.609
2.128LeuMet: 2.128 ± 0.34
6.864LeuAsn: 6.864 ± 0.812
2.471LeuPro: 2.471 ± 0.374
2.677LeuGln: 2.677 ± 0.463
3.638LeuArg: 3.638 ± 0.54
5.629LeuSer: 5.629 ± 0.701
4.53LeuThr: 4.53 ± 0.539
4.393LeuVal: 4.393 ± 0.528
0.686LeuTrp: 0.686 ± 0.206
3.295LeuTyr: 3.295 ± 0.585
0.0LeuXaa: 0.0 ± 0.0
Met
1.442MetAla: 1.442 ± 0.262
0.069MetCys: 0.069 ± 0.069
0.755MetAsp: 0.755 ± 0.244
1.167MetGlu: 1.167 ± 0.25
0.892MetPhe: 0.892 ± 0.253
1.03MetGly: 1.03 ± 0.33
0.549MetHis: 0.549 ± 0.169
1.853MetIle: 1.853 ± 0.365
2.952MetLys: 2.952 ± 0.478
1.579MetLeu: 1.579 ± 0.304
0.481MetMet: 0.481 ± 0.183
1.785MetAsn: 1.785 ± 0.358
0.275MetPro: 0.275 ± 0.162
1.03MetGln: 1.03 ± 0.273
0.618MetArg: 0.618 ± 0.215
1.167MetSer: 1.167 ± 0.322
1.304MetThr: 1.304 ± 0.286
0.961MetVal: 0.961 ± 0.273
0.137MetTrp: 0.137 ± 0.085
0.755MetTyr: 0.755 ± 0.235
0.0MetXaa: 0.0 ± 0.0
Asn
3.638AsnAla: 3.638 ± 0.622
0.892AsnCys: 0.892 ± 0.283
3.226AsnAsp: 3.226 ± 0.463
5.697AsnGlu: 5.697 ± 0.691
3.089AsnPhe: 3.089 ± 0.541
5.423AsnGly: 5.423 ± 0.874
1.304AsnHis: 1.304 ± 0.342
4.942AsnIle: 4.942 ± 0.629
8.1AsnLys: 8.1 ± 0.915
6.727AsnLeu: 6.727 ± 0.637
1.716AsnMet: 1.716 ± 0.358
4.736AsnAsn: 4.736 ± 0.545
2.265AsnPro: 2.265 ± 0.401
1.785AsnGln: 1.785 ± 0.363
3.158AsnArg: 3.158 ± 0.546
4.736AsnSer: 4.736 ± 0.641
3.981AsnThr: 3.981 ± 0.628
3.569AsnVal: 3.569 ± 0.506
0.824AsnTrp: 0.824 ± 0.284
3.844AsnTyr: 3.844 ± 0.611
0.0AsnXaa: 0.0 ± 0.0
Pro
0.343ProAla: 0.343 ± 0.168
0.069ProCys: 0.069 ± 0.067
1.098ProAsp: 1.098 ± 0.349
1.647ProGlu: 1.647 ± 0.287
1.785ProPhe: 1.785 ± 0.373
0.275ProGly: 0.275 ± 0.127
0.275ProHis: 0.275 ± 0.143
1.922ProIle: 1.922 ± 0.381
2.54ProLys: 2.54 ± 0.44
2.471ProLeu: 2.471 ± 0.462
0.755ProMet: 0.755 ± 0.266
1.853ProAsn: 1.853 ± 0.329
0.412ProPro: 0.412 ± 0.168
0.892ProGln: 0.892 ± 0.28
0.824ProArg: 0.824 ± 0.218
1.098ProSer: 1.098 ± 0.28
1.785ProThr: 1.785 ± 0.298
0.686ProVal: 0.686 ± 0.241
0.069ProTrp: 0.069 ± 0.062
1.51ProTyr: 1.51 ± 0.39
0.0ProXaa: 0.0 ± 0.0
Gln
2.059GlnAla: 2.059 ± 0.53
0.206GlnCys: 0.206 ± 0.114
1.579GlnAsp: 1.579 ± 0.367
3.02GlnGlu: 3.02 ± 0.511
1.098GlnPhe: 1.098 ± 0.237
1.51GlnGly: 1.51 ± 0.353
0.549GlnHis: 0.549 ± 0.268
2.677GlnIle: 2.677 ± 0.458
3.569GlnLys: 3.569 ± 0.619
2.334GlnLeu: 2.334 ± 0.436
1.03GlnMet: 1.03 ± 0.348
1.922GlnAsn: 1.922 ± 0.304
0.686GlnPro: 0.686 ± 0.208
0.961GlnGln: 0.961 ± 0.302
1.442GlnArg: 1.442 ± 0.331
1.922GlnSer: 1.922 ± 0.438
1.579GlnThr: 1.579 ± 0.332
1.853GlnVal: 1.853 ± 0.321
0.343GlnTrp: 0.343 ± 0.142
1.167GlnTyr: 1.167 ± 0.26
0.0GlnXaa: 0.0 ± 0.0
Arg
1.716ArgAla: 1.716 ± 0.284
0.412ArgCys: 0.412 ± 0.169
2.265ArgAsp: 2.265 ± 0.353
2.128ArgGlu: 2.128 ± 0.338
1.51ArgPhe: 1.51 ± 0.383
2.059ArgGly: 2.059 ± 0.47
0.275ArgHis: 0.275 ± 0.176
3.775ArgIle: 3.775 ± 0.509
3.707ArgLys: 3.707 ± 0.588
3.981ArgLeu: 3.981 ± 0.45
0.892ArgMet: 0.892 ± 0.188
1.922ArgAsn: 1.922 ± 0.383
0.686ArgPro: 0.686 ± 0.184
1.442ArgGln: 1.442 ± 0.308
1.51ArgArg: 1.51 ± 0.277
1.922ArgSer: 1.922 ± 0.392
1.785ArgThr: 1.785 ± 0.388
2.471ArgVal: 2.471 ± 0.384
0.343ArgTrp: 0.343 ± 0.135
2.54ArgTyr: 2.54 ± 0.483
0.0ArgXaa: 0.0 ± 0.0
Ser
1.991SerAla: 1.991 ± 0.326
0.618SerCys: 0.618 ± 0.189
4.256SerAsp: 4.256 ± 0.654
5.766SerGlu: 5.766 ± 0.654
3.226SerPhe: 3.226 ± 0.491
2.608SerGly: 2.608 ± 0.529
1.03SerHis: 1.03 ± 0.349
5.354SerIle: 5.354 ± 0.587
6.658SerLys: 6.658 ± 0.89
5.423SerLeu: 5.423 ± 0.618
1.373SerMet: 1.373 ± 0.526
5.148SerAsn: 5.148 ± 0.658
0.961SerPro: 0.961 ± 0.261
1.922SerGln: 1.922 ± 0.328
2.197SerArg: 2.197 ± 0.551
4.599SerSer: 4.599 ± 0.607
4.05SerThr: 4.05 ± 0.743
3.775SerVal: 3.775 ± 0.517
0.618SerTrp: 0.618 ± 0.245
2.746SerTyr: 2.746 ± 0.476
0.0SerXaa: 0.0 ± 0.0
Thr
2.677ThrAla: 2.677 ± 0.562
0.755ThrCys: 0.755 ± 0.282
4.187ThrAsp: 4.187 ± 0.648
3.501ThrGlu: 3.501 ± 0.467
2.54ThrPhe: 2.54 ± 0.441
3.089ThrGly: 3.089 ± 0.698
0.686ThrHis: 0.686 ± 0.204
4.668ThrIle: 4.668 ± 0.718
5.903ThrLys: 5.903 ± 0.652
4.05ThrLeu: 4.05 ± 0.569
1.167ThrMet: 1.167 ± 0.373
4.462ThrAsn: 4.462 ± 0.7
1.304ThrPro: 1.304 ± 0.347
2.128ThrGln: 2.128 ± 0.385
2.059ThrArg: 2.059 ± 0.315
3.432ThrSer: 3.432 ± 0.689
4.119ThrThr: 4.119 ± 0.623
3.501ThrVal: 3.501 ± 0.606
0.412ThrTrp: 0.412 ± 0.189
2.677ThrTyr: 2.677 ± 0.454
0.0ThrXaa: 0.0 ± 0.0
Val
2.608ValAla: 2.608 ± 0.391
0.618ValCys: 0.618 ± 0.203
3.913ValAsp: 3.913 ± 0.599
3.295ValGlu: 3.295 ± 0.537
2.746ValPhe: 2.746 ± 0.393
3.569ValGly: 3.569 ± 0.483
0.755ValHis: 0.755 ± 0.259
4.599ValIle: 4.599 ± 0.567
6.658ValLys: 6.658 ± 0.662
4.393ValLeu: 4.393 ± 0.634
0.343ValMet: 0.343 ± 0.137
3.501ValAsn: 3.501 ± 0.46
1.442ValPro: 1.442 ± 0.283
1.647ValGln: 1.647 ± 0.408
1.716ValArg: 1.716 ± 0.329
4.874ValSer: 4.874 ± 0.728
2.883ValThr: 2.883 ± 0.618
4.325ValVal: 4.325 ± 0.498
0.549ValTrp: 0.549 ± 0.206
1.922ValTyr: 1.922 ± 0.433
0.0ValXaa: 0.0 ± 0.0
Trp
0.892TrpAla: 0.892 ± 0.348
0.069TrpCys: 0.069 ± 0.065
1.098TrpAsp: 1.098 ± 0.343
0.412TrpGlu: 0.412 ± 0.157
0.755TrpPhe: 0.755 ± 0.22
0.275TrpGly: 0.275 ± 0.12
0.069TrpHis: 0.069 ± 0.071
1.098TrpIle: 1.098 ± 0.261
0.686TrpLys: 0.686 ± 0.167
1.167TrpLeu: 1.167 ± 0.353
0.206TrpMet: 0.206 ± 0.112
1.03TrpAsn: 1.03 ± 0.267
0.069TrpPro: 0.069 ± 0.06
0.343TrpGln: 0.343 ± 0.139
0.481TrpArg: 0.481 ± 0.19
0.549TrpSer: 0.549 ± 0.205
0.686TrpThr: 0.686 ± 0.203
0.961TrpVal: 0.961 ± 0.224
0.0TrpTrp: 0.0 ± 0.0
0.137TrpTyr: 0.137 ± 0.082
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.304TyrAla: 1.304 ± 0.321
0.618TyrCys: 0.618 ± 0.274
2.608TyrAsp: 2.608 ± 0.388
4.119TyrGlu: 4.119 ± 0.52
2.403TyrPhe: 2.403 ± 0.479
2.54TyrGly: 2.54 ± 0.423
0.549TyrHis: 0.549 ± 0.166
3.707TyrIle: 3.707 ± 0.483
4.325TyrLys: 4.325 ± 0.654
4.119TyrLeu: 4.119 ± 0.548
0.824TyrMet: 0.824 ± 0.243
3.089TyrAsn: 3.089 ± 0.386
1.236TyrPro: 1.236 ± 0.308
2.334TyrGln: 2.334 ± 0.38
1.442TyrArg: 1.442 ± 0.301
2.059TyrSer: 2.059 ± 0.314
2.608TyrThr: 2.608 ± 0.434
1.785TyrVal: 1.785 ± 0.372
0.892TyrTrp: 0.892 ± 0.223
2.197TyrTyr: 2.197 ± 0.419
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (14569 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski