Pseudomonas phage phiK7A1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 174 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7H0XG00|A0A7H0XG00_9CAUD Uncharacterized protein OS=Pseudomonas phage phiK7A1 OX=2759194 GN=phiK7A1_152 PE=4 SV=1
MM1 pKa = 7.97AIPAGVKK8 pKa = 10.31VGDD11 pKa = 3.65VLVAVDD17 pKa = 3.9TVGNNIGDD25 pKa = 4.03IYY27 pKa = 10.17PAGRR31 pKa = 11.84EE32 pKa = 3.88FRR34 pKa = 11.84VCRR37 pKa = 11.84MDD39 pKa = 3.27GDD41 pKa = 3.96RR42 pKa = 11.84YY43 pKa = 10.5LALEE47 pKa = 4.19PTDD50 pKa = 5.49YY51 pKa = 11.04SDD53 pKa = 4.35CDD55 pKa = 2.6IWFYY59 pKa = 11.26FFDD62 pKa = 4.31SYY64 pKa = 11.99SEE66 pKa = 3.89VDD68 pKa = 3.08NTDD71 pKa = 2.93RR72 pKa = 11.84FVIQQQ77 pKa = 3.22

Molecular weight: 8.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7H0XFU9|A0A7H0XFU9_9CAUD Uncharacterized protein OS=Pseudomonas phage phiK7A1 OX=2759194 GN=phiK7A1_101 PE=4 SV=1
MM1 pKa = 7.32QPAHH5 pKa = 6.91DD6 pKa = 4.74LKK8 pKa = 11.27FHH10 pKa = 7.57GIRR13 pKa = 11.84QRR15 pKa = 11.84QTPRR19 pKa = 11.84HH20 pKa = 4.66VLGRR24 pKa = 11.84EE25 pKa = 3.59SCMASLGIGLPPLRR39 pKa = 11.84NSVPQEE45 pKa = 3.75KK46 pKa = 10.34QYY48 pKa = 11.59VLSSSKK54 pKa = 11.04

Molecular weight: 6.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

174

0

174

29006

43

892

166.7

18.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.922 ± 0.264

1.355 ± 0.108

6.033 ± 0.183

6.319 ± 0.238

3.83 ± 0.127

7.136 ± 0.219

2.096 ± 0.116

5.437 ± 0.141

5.678 ± 0.226

8.205 ± 0.181

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.003 ± 0.131

4.141 ± 0.147

3.799 ± 0.158

3.934 ± 0.128

4.761 ± 0.134

5.926 ± 0.227

6.785 ± 0.256

7.223 ± 0.2

1.579 ± 0.096

3.837 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski