Amino acid dipepetide frequency for Pseudomonas phage phiK7A1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.033AlaAla: 9.033 ± 0.78
1.034AlaCys: 1.034 ± 0.205
5.068AlaAsp: 5.068 ± 0.433
4.999AlaGlu: 4.999 ± 0.495
3.137AlaPhe: 3.137 ± 0.376
5.896AlaGly: 5.896 ± 0.562
2.275AlaHis: 2.275 ± 0.335
5.309AlaIle: 5.309 ± 0.463
5.068AlaLys: 5.068 ± 0.608
7.688AlaLeu: 7.688 ± 0.549
2.517AlaMet: 2.517 ± 0.271
3.379AlaAsn: 3.379 ± 0.455
3.275AlaPro: 3.275 ± 0.44
4.516AlaGln: 4.516 ± 0.383
5.034AlaArg: 5.034 ± 0.55
4.137AlaSer: 4.137 ± 0.343
6.275AlaThr: 6.275 ± 0.627
6.275AlaVal: 6.275 ± 0.575
1.172AlaTrp: 1.172 ± 0.198
3.137AlaTyr: 3.137 ± 0.286
0.0AlaXaa: 0.0 ± 0.0
Cys
1.31CysAla: 1.31 ± 0.246
0.069CysCys: 0.069 ± 0.053
0.931CysAsp: 0.931 ± 0.18
0.862CysGlu: 0.862 ± 0.209
0.414CysPhe: 0.414 ± 0.125
1.0CysGly: 1.0 ± 0.22
0.379CysHis: 0.379 ± 0.111
0.414CysIle: 0.414 ± 0.143
0.517CysLys: 0.517 ± 0.132
1.138CysLeu: 1.138 ± 0.187
0.552CysMet: 0.552 ± 0.139
0.758CysAsn: 0.758 ± 0.155
0.552CysPro: 0.552 ± 0.163
0.345CysGln: 0.345 ± 0.131
0.655CysArg: 0.655 ± 0.165
1.138CysSer: 1.138 ± 0.202
0.896CysThr: 0.896 ± 0.21
1.034CysVal: 1.034 ± 0.23
0.103CysTrp: 0.103 ± 0.062
0.483CysTyr: 0.483 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
5.585AspAla: 5.585 ± 0.398
0.896AspCys: 0.896 ± 0.197
3.827AspAsp: 3.827 ± 0.418
3.103AspGlu: 3.103 ± 0.366
2.689AspPhe: 2.689 ± 0.267
4.93AspGly: 4.93 ± 0.428
1.172AspHis: 1.172 ± 0.203
2.931AspIle: 2.931 ± 0.254
3.551AspLys: 3.551 ± 0.362
4.723AspLeu: 4.723 ± 0.328
1.793AspMet: 1.793 ± 0.276
2.724AspAsn: 2.724 ± 0.346
2.275AspPro: 2.275 ± 0.271
1.551AspGln: 1.551 ± 0.254
3.034AspArg: 3.034 ± 0.324
3.379AspSer: 3.379 ± 0.41
4.103AspThr: 4.103 ± 0.412
3.93AspVal: 3.93 ± 0.39
1.069AspTrp: 1.069 ± 0.181
3.068AspTyr: 3.068 ± 0.388
0.0AspXaa: 0.0 ± 0.0
Glu
6.275GluAla: 6.275 ± 0.477
1.069GluCys: 1.069 ± 0.203
3.999GluAsp: 3.999 ± 0.376
4.689GluGlu: 4.689 ± 0.435
2.827GluPhe: 2.827 ± 0.382
3.482GluGly: 3.482 ± 0.449
1.034GluHis: 1.034 ± 0.165
3.413GluIle: 3.413 ± 0.422
3.31GluLys: 3.31 ± 0.441
6.275GluLeu: 6.275 ± 0.585
2.344GluMet: 2.344 ± 0.293
2.138GluAsn: 2.138 ± 0.234
1.379GluPro: 1.379 ± 0.231
2.931GluGln: 2.931 ± 0.324
3.172GluArg: 3.172 ± 0.331
2.62GluSer: 2.62 ± 0.299
3.103GluThr: 3.103 ± 0.283
4.965GluVal: 4.965 ± 0.452
1.379GluTrp: 1.379 ± 0.238
2.793GluTyr: 2.793 ± 0.287
0.0GluXaa: 0.0 ± 0.0
Phe
3.275PheAla: 3.275 ± 0.342
0.483PheCys: 0.483 ± 0.129
2.344PheAsp: 2.344 ± 0.327
2.689PheGlu: 2.689 ± 0.264
1.551PhePhe: 1.551 ± 0.232
2.931PheGly: 2.931 ± 0.365
0.552PheHis: 0.552 ± 0.141
2.379PheIle: 2.379 ± 0.275
2.344PheLys: 2.344 ± 0.295
2.999PheLeu: 2.999 ± 0.332
1.172PheMet: 1.172 ± 0.185
1.896PheAsn: 1.896 ± 0.293
1.586PhePro: 1.586 ± 0.191
1.31PheGln: 1.31 ± 0.206
1.414PheArg: 1.414 ± 0.223
2.069PheSer: 2.069 ± 0.282
3.034PheThr: 3.034 ± 0.388
2.241PheVal: 2.241 ± 0.303
0.655PheTrp: 0.655 ± 0.177
1.379PheTyr: 1.379 ± 0.202
0.0PheXaa: 0.0 ± 0.0
Gly
4.792GlyAla: 4.792 ± 0.476
1.0GlyCys: 1.0 ± 0.19
4.448GlyAsp: 4.448 ± 0.431
4.344GlyGlu: 4.344 ± 0.424
3.62GlyPhe: 3.62 ± 0.359
4.896GlyGly: 4.896 ± 0.382
1.483GlyHis: 1.483 ± 0.282
3.689GlyIle: 3.689 ± 0.358
3.586GlyLys: 3.586 ± 0.394
5.551GlyLeu: 5.551 ± 0.49
2.103GlyMet: 2.103 ± 0.293
3.034GlyAsn: 3.034 ± 0.317
2.0GlyPro: 2.0 ± 0.258
2.551GlyGln: 2.551 ± 0.303
3.413GlyArg: 3.413 ± 0.361
5.309GlySer: 5.309 ± 0.456
4.896GlyThr: 4.896 ± 0.546
5.206GlyVal: 5.206 ± 0.389
1.689GlyTrp: 1.689 ± 0.232
3.758GlyTyr: 3.758 ± 0.44
0.0GlyXaa: 0.0 ± 0.0
His
1.62HisAla: 1.62 ± 0.251
0.414HisCys: 0.414 ± 0.142
1.448HisAsp: 1.448 ± 0.252
1.414HisGlu: 1.414 ± 0.216
1.0HisPhe: 1.0 ± 0.237
1.414HisGly: 1.414 ± 0.282
0.345HisHis: 0.345 ± 0.128
1.345HisIle: 1.345 ± 0.214
1.483HisLys: 1.483 ± 0.292
1.448HisLeu: 1.448 ± 0.221
0.724HisMet: 0.724 ± 0.183
1.103HisAsn: 1.103 ± 0.211
0.827HisPro: 0.827 ± 0.154
0.552HisGln: 0.552 ± 0.143
0.621HisArg: 0.621 ± 0.143
1.103HisSer: 1.103 ± 0.197
1.793HisThr: 1.793 ± 0.24
1.103HisVal: 1.103 ± 0.193
0.414HisTrp: 0.414 ± 0.135
0.793HisTyr: 0.793 ± 0.168
0.0HisXaa: 0.0 ± 0.0
Ile
4.965IleAla: 4.965 ± 0.473
0.621IleCys: 0.621 ± 0.151
3.62IleAsp: 3.62 ± 0.315
3.827IleGlu: 3.827 ± 0.41
1.345IlePhe: 1.345 ± 0.225
3.758IleGly: 3.758 ± 0.342
1.034IleHis: 1.034 ± 0.21
2.793IleIle: 2.793 ± 0.288
3.413IleLys: 3.413 ± 0.408
4.413IleLeu: 4.413 ± 0.397
1.276IleMet: 1.276 ± 0.185
2.31IleAsn: 2.31 ± 0.255
2.62IlePro: 2.62 ± 0.26
1.965IleGln: 1.965 ± 0.244
2.965IleArg: 2.965 ± 0.313
3.275IleSer: 3.275 ± 0.385
4.034IleThr: 4.034 ± 0.377
3.965IleVal: 3.965 ± 0.426
0.69IleTrp: 0.69 ± 0.154
1.483IleTyr: 1.483 ± 0.197
0.0IleXaa: 0.0 ± 0.0
Lys
6.24LysAla: 6.24 ± 0.689
0.448LysCys: 0.448 ± 0.12
3.172LysAsp: 3.172 ± 0.377
3.792LysGlu: 3.792 ± 0.397
1.793LysPhe: 1.793 ± 0.23
4.551LysGly: 4.551 ± 0.415
1.172LysHis: 1.172 ± 0.279
2.655LysIle: 2.655 ± 0.278
2.862LysLys: 2.862 ± 0.402
5.24LysLeu: 5.24 ± 0.467
2.379LysMet: 2.379 ± 0.298
2.069LysAsn: 2.069 ± 0.267
2.586LysPro: 2.586 ± 0.311
2.517LysGln: 2.517 ± 0.327
2.793LysArg: 2.793 ± 0.291
2.517LysSer: 2.517 ± 0.294
2.379LysThr: 2.379 ± 0.294
4.62LysVal: 4.62 ± 0.35
0.69LysTrp: 0.69 ± 0.138
2.31LysTyr: 2.31 ± 0.362
0.0LysXaa: 0.0 ± 0.0
Leu
8.723LeuAla: 8.723 ± 0.657
0.931LeuCys: 0.931 ± 0.191
5.827LeuAsp: 5.827 ± 0.447
5.896LeuGlu: 5.896 ± 0.527
3.275LeuPhe: 3.275 ± 0.331
5.999LeuGly: 5.999 ± 0.516
1.827LeuHis: 1.827 ± 0.252
3.93LeuIle: 3.93 ± 0.41
4.999LeuLys: 4.999 ± 0.402
5.551LeuLeu: 5.551 ± 0.536
2.551LeuMet: 2.551 ± 0.323
2.931LeuAsn: 2.931 ± 0.281
3.275LeuPro: 3.275 ± 0.288
3.172LeuGln: 3.172 ± 0.427
3.999LeuArg: 3.999 ± 0.411
5.103LeuSer: 5.103 ± 0.401
5.447LeuThr: 5.447 ± 0.568
5.068LeuVal: 5.068 ± 0.415
0.896LeuTrp: 0.896 ± 0.18
2.655LeuTyr: 2.655 ± 0.321
0.0LeuXaa: 0.0 ± 0.0
Met
2.999MetAla: 2.999 ± 0.337
0.517MetCys: 0.517 ± 0.141
1.586MetAsp: 1.586 ± 0.216
1.724MetGlu: 1.724 ± 0.277
0.758MetPhe: 0.758 ± 0.169
1.62MetGly: 1.62 ± 0.282
0.586MetHis: 0.586 ± 0.174
1.62MetIle: 1.62 ± 0.229
2.517MetLys: 2.517 ± 0.286
2.413MetLeu: 2.413 ± 0.265
1.138MetMet: 1.138 ± 0.207
0.931MetAsn: 0.931 ± 0.157
1.241MetPro: 1.241 ± 0.189
1.103MetGln: 1.103 ± 0.218
1.172MetArg: 1.172 ± 0.199
2.827MetSer: 2.827 ± 0.275
2.172MetThr: 2.172 ± 0.249
1.689MetVal: 1.689 ± 0.207
0.414MetTrp: 0.414 ± 0.12
1.0MetTyr: 1.0 ± 0.204
0.0MetXaa: 0.0 ± 0.0
Asn
2.827AsnAla: 2.827 ± 0.319
0.483AsnCys: 0.483 ± 0.135
2.344AsnAsp: 2.344 ± 0.282
1.724AsnGlu: 1.724 ± 0.225
1.207AsnPhe: 1.207 ± 0.21
3.103AsnGly: 3.103 ± 0.338
0.758AsnHis: 0.758 ± 0.173
3.034AsnIle: 3.034 ± 0.348
3.103AsnLys: 3.103 ± 0.3
2.758AsnLeu: 2.758 ± 0.39
0.758AsnMet: 0.758 ± 0.168
1.965AsnAsn: 1.965 ± 0.283
2.482AsnPro: 2.482 ± 0.311
1.414AsnGln: 1.414 ± 0.213
1.724AsnArg: 1.724 ± 0.299
2.551AsnSer: 2.551 ± 0.317
3.275AsnThr: 3.275 ± 0.344
3.413AsnVal: 3.413 ± 0.349
0.862AsnTrp: 0.862 ± 0.14
1.62AsnTyr: 1.62 ± 0.255
0.0AsnXaa: 0.0 ± 0.0
Pro
3.413ProAla: 3.413 ± 0.333
0.621ProCys: 0.621 ± 0.154
2.138ProAsp: 2.138 ± 0.32
3.275ProGlu: 3.275 ± 0.329
1.276ProPhe: 1.276 ± 0.213
2.034ProGly: 2.034 ± 0.284
1.103ProHis: 1.103 ± 0.203
1.207ProIle: 1.207 ± 0.225
1.862ProLys: 1.862 ± 0.247
3.551ProLeu: 3.551 ± 0.34
0.931ProMet: 0.931 ± 0.181
1.551ProAsn: 1.551 ± 0.268
0.862ProPro: 0.862 ± 0.224
1.931ProGln: 1.931 ± 0.264
1.517ProArg: 1.517 ± 0.185
2.344ProSer: 2.344 ± 0.323
2.999ProThr: 2.999 ± 0.38
3.586ProVal: 3.586 ± 0.384
0.552ProTrp: 0.552 ± 0.16
1.207ProTyr: 1.207 ± 0.196
0.0ProXaa: 0.0 ± 0.0
Gln
3.586GlnAla: 3.586 ± 0.443
0.69GlnCys: 0.69 ± 0.154
2.207GlnAsp: 2.207 ± 0.298
2.862GlnGlu: 2.862 ± 0.29
1.965GlnPhe: 1.965 ± 0.249
2.379GlnGly: 2.379 ± 0.281
0.758GlnHis: 0.758 ± 0.162
2.344GlnIle: 2.344 ± 0.275
1.827GlnLys: 1.827 ± 0.232
3.206GlnLeu: 3.206 ± 0.352
1.241GlnMet: 1.241 ± 0.221
1.379GlnAsn: 1.379 ± 0.205
1.103GlnPro: 1.103 ± 0.199
2.138GlnGln: 2.138 ± 0.281
2.413GlnArg: 2.413 ± 0.247
2.241GlnSer: 2.241 ± 0.265
2.275GlnThr: 2.275 ± 0.283
2.655GlnVal: 2.655 ± 0.291
0.655GlnTrp: 0.655 ± 0.156
1.414GlnTyr: 1.414 ± 0.224
0.0GlnXaa: 0.0 ± 0.0
Arg
3.689ArgAla: 3.689 ± 0.336
0.758ArgCys: 0.758 ± 0.167
2.482ArgAsp: 2.482 ± 0.261
2.999ArgGlu: 2.999 ± 0.328
1.965ArgPhe: 1.965 ± 0.267
3.31ArgGly: 3.31 ± 0.361
1.069ArgHis: 1.069 ± 0.21
2.896ArgIle: 2.896 ± 0.307
2.413ArgLys: 2.413 ± 0.281
4.861ArgLeu: 4.861 ± 0.415
1.379ArgMet: 1.379 ± 0.21
2.138ArgAsn: 2.138 ± 0.219
1.724ArgPro: 1.724 ± 0.211
1.586ArgGln: 1.586 ± 0.235
2.275ArgArg: 2.275 ± 0.334
2.724ArgSer: 2.724 ± 0.291
2.758ArgThr: 2.758 ± 0.33
3.551ArgVal: 3.551 ± 0.335
0.862ArgTrp: 0.862 ± 0.178
2.172ArgTyr: 2.172 ± 0.255
0.0ArgXaa: 0.0 ± 0.0
Ser
4.792SerAla: 4.792 ± 0.412
0.69SerCys: 0.69 ± 0.174
3.517SerAsp: 3.517 ± 0.39
3.068SerGlu: 3.068 ± 0.267
1.931SerPhe: 1.931 ± 0.249
5.585SerGly: 5.585 ± 0.581
1.379SerHis: 1.379 ± 0.209
3.241SerIle: 3.241 ± 0.377
3.034SerLys: 3.034 ± 0.364
5.172SerLeu: 5.172 ± 0.5
1.724SerMet: 1.724 ± 0.225
2.275SerAsn: 2.275 ± 0.321
2.551SerPro: 2.551 ± 0.288
2.31SerGln: 2.31 ± 0.351
2.413SerArg: 2.413 ± 0.324
4.654SerSer: 4.654 ± 1.08
3.861SerThr: 3.861 ± 0.494
3.792SerVal: 3.792 ± 0.446
1.034SerTrp: 1.034 ± 0.196
2.241SerTyr: 2.241 ± 0.302
0.0SerXaa: 0.0 ± 0.0
Thr
5.792ThrAla: 5.792 ± 0.571
1.034ThrCys: 1.034 ± 0.206
3.344ThrAsp: 3.344 ± 0.408
3.93ThrGlu: 3.93 ± 0.327
2.482ThrPhe: 2.482 ± 0.297
5.516ThrGly: 5.516 ± 0.485
1.551ThrHis: 1.551 ± 0.234
3.792ThrIle: 3.792 ± 0.351
3.275ThrLys: 3.275 ± 0.323
5.172ThrLeu: 5.172 ± 0.379
2.069ThrMet: 2.069 ± 0.234
2.758ThrAsn: 2.758 ± 0.286
3.103ThrPro: 3.103 ± 0.353
3.068ThrGln: 3.068 ± 0.375
3.034ThrArg: 3.034 ± 0.297
3.93ThrSer: 3.93 ± 0.412
4.827ThrThr: 4.827 ± 0.519
5.551ThrVal: 5.551 ± 0.531
0.931ThrTrp: 0.931 ± 0.207
2.689ThrTyr: 2.689 ± 0.35
0.0ThrXaa: 0.0 ± 0.0
Val
6.206ValAla: 6.206 ± 0.527
1.207ValCys: 1.207 ± 0.233
4.379ValAsp: 4.379 ± 0.366
4.413ValGlu: 4.413 ± 0.417
2.758ValPhe: 2.758 ± 0.346
5.413ValGly: 5.413 ± 0.363
1.31ValHis: 1.31 ± 0.23
4.206ValIle: 4.206 ± 0.388
4.275ValLys: 4.275 ± 0.438
5.482ValLeu: 5.482 ± 0.444
1.965ValMet: 1.965 ± 0.261
3.517ValAsn: 3.517 ± 0.406
2.482ValPro: 2.482 ± 0.274
2.827ValGln: 2.827 ± 0.294
3.206ValArg: 3.206 ± 0.363
3.689ValSer: 3.689 ± 0.396
5.447ValThr: 5.447 ± 0.462
5.896ValVal: 5.896 ± 0.465
1.0ValTrp: 1.0 ± 0.184
2.551ValTyr: 2.551 ± 0.329
0.0ValXaa: 0.0 ± 0.0
Trp
1.241TrpAla: 1.241 ± 0.212
0.172TrpCys: 0.172 ± 0.082
1.069TrpAsp: 1.069 ± 0.179
1.103TrpGlu: 1.103 ± 0.199
0.655TrpPhe: 0.655 ± 0.163
0.586TrpGly: 0.586 ± 0.133
0.345TrpHis: 0.345 ± 0.099
0.827TrpIle: 0.827 ± 0.2
0.862TrpLys: 0.862 ± 0.157
1.862TrpLeu: 1.862 ± 0.242
0.31TrpMet: 0.31 ± 0.123
0.862TrpAsn: 0.862 ± 0.169
0.586TrpPro: 0.586 ± 0.127
0.586TrpGln: 0.586 ± 0.155
0.655TrpArg: 0.655 ± 0.147
1.034TrpSer: 1.034 ± 0.174
1.103TrpThr: 1.103 ± 0.225
1.069TrpVal: 1.069 ± 0.272
0.31TrpTrp: 0.31 ± 0.101
0.552TrpTyr: 0.552 ± 0.136
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.862TyrAla: 2.862 ± 0.318
0.414TyrCys: 0.414 ± 0.201
2.586TyrAsp: 2.586 ± 0.342
2.482TyrGlu: 2.482 ± 0.345
1.655TyrPhe: 1.655 ± 0.209
2.965TyrGly: 2.965 ± 0.306
0.827TyrHis: 0.827 ± 0.18
2.344TyrIle: 2.344 ± 0.302
2.482TyrLys: 2.482 ± 0.29
2.551TyrLeu: 2.551 ± 0.271
1.069TyrMet: 1.069 ± 0.19
1.689TyrAsn: 1.689 ± 0.254
1.483TyrPro: 1.483 ± 0.239
0.965TyrGln: 0.965 ± 0.207
2.103TyrArg: 2.103 ± 0.225
2.62TyrSer: 2.62 ± 0.387
3.172TyrThr: 3.172 ± 0.321
2.62TyrVal: 2.62 ± 0.294
0.414TyrTrp: 0.414 ± 0.11
1.069TyrTyr: 1.069 ± 0.164
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 174 proteins (29006 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski