Serratia phage Eta

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9VYK9|R9VYK9_9CAUD Uncharacterized protein OS=Serratia phage Eta OX=1282995 GN=Eta_0049 PE=4 SV=1
MM1 pKa = 7.56SGFFLVSDD9 pKa = 3.94VPARR13 pKa = 11.84RR14 pKa = 11.84VVQYY18 pKa = 10.61KK19 pKa = 9.68RR20 pKa = 11.84VSSTVDD26 pKa = 3.08GAIYY30 pKa = 10.18LADD33 pKa = 4.9DD34 pKa = 3.91SLLGKK39 pKa = 10.26AVDD42 pKa = 4.0AMPFVDD48 pKa = 4.22KK49 pKa = 10.44TGLGIAVGGTLYY61 pKa = 10.14EE62 pKa = 4.3VPYY65 pKa = 10.63LPDD68 pKa = 3.85AGAVYY73 pKa = 9.22FVVQPYY79 pKa = 9.33GASGNADD86 pKa = 3.77ALHH89 pKa = 6.28MEE91 pKa = 4.56VEE93 pKa = 4.53AKK95 pKa = 10.25AGKK98 pKa = 10.08APYY101 pKa = 9.83SYY103 pKa = 10.69QWYY106 pKa = 10.03KK107 pKa = 11.22DD108 pKa = 3.45DD109 pKa = 3.82KK110 pKa = 11.1QVVNVPAASASLTVTEE126 pKa = 4.6TGKK129 pKa = 9.9YY130 pKa = 7.32WCVATDD136 pKa = 3.48ADD138 pKa = 4.33GVQAVSEE145 pKa = 4.12AADD148 pKa = 3.33VEE150 pKa = 4.49FTEE153 pKa = 4.78EE154 pKa = 3.9VKK156 pKa = 10.94

Molecular weight: 16.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9VX71|R9VX71_9CAUD Terminase large subunit OS=Serratia phage Eta OX=1282995 GN=terL PE=4 SV=1
MM1 pKa = 7.27FKK3 pKa = 10.39KK4 pKa = 10.6GQLLSNPATGAYY16 pKa = 9.95VEE18 pKa = 4.36VLAWPWVRR26 pKa = 11.84LVRR29 pKa = 11.84PGRR32 pKa = 11.84LNGYY36 pKa = 8.15RR37 pKa = 11.84AGVEE41 pKa = 4.29FHH43 pKa = 7.06ASNNFTLIGNNYY55 pKa = 8.22KK56 pKa = 10.85AKK58 pKa = 10.47GG59 pKa = 3.47

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

13368

34

911

193.7

21.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.765 ± 0.651

1.257 ± 0.142

5.932 ± 0.187

6.478 ± 0.408

3.224 ± 0.228

7.264 ± 0.353

1.721 ± 0.156

5.91 ± 0.308

5.715 ± 0.339

7.346 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.813 ± 0.182

4.511 ± 0.254

3.606 ± 0.23

4.062 ± 0.344

5.416 ± 0.265

6.695 ± 0.332

5.88 ± 0.334

7.009 ± 0.3

1.287 ± 0.16

3.112 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski