Amino acid dipepetide frequency for Serratia phage Eta

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.868AlaAla: 12.868 ± 2.037
0.898AlaCys: 0.898 ± 0.241
6.359AlaAsp: 6.359 ± 0.802
8.603AlaGlu: 8.603 ± 1.356
3.366AlaPhe: 3.366 ± 0.478
7.631AlaGly: 7.631 ± 0.942
2.095AlaHis: 2.095 ± 0.465
6.957AlaIle: 6.957 ± 0.659
6.284AlaLys: 6.284 ± 1.09
8.229AlaLeu: 8.229 ± 0.889
3.067AlaMet: 3.067 ± 0.479
3.815AlaAsn: 3.815 ± 0.535
2.618AlaPro: 2.618 ± 0.504
4.339AlaGln: 4.339 ± 0.79
5.386AlaArg: 5.386 ± 0.752
6.509AlaSer: 6.509 ± 0.807
6.957AlaThr: 6.957 ± 0.573
6.509AlaVal: 6.509 ± 0.867
1.496AlaTrp: 1.496 ± 0.322
3.666AlaTyr: 3.666 ± 0.527
0.0AlaXaa: 0.0 ± 0.0
Cys
1.197CysAla: 1.197 ± 0.367
0.15CysCys: 0.15 ± 0.169
0.598CysAsp: 0.598 ± 0.214
0.823CysGlu: 0.823 ± 0.273
0.299CysPhe: 0.299 ± 0.147
1.347CysGly: 1.347 ± 0.318
0.299CysHis: 0.299 ± 0.123
0.673CysIle: 0.673 ± 0.21
0.598CysLys: 0.598 ± 0.196
0.748CysLeu: 0.748 ± 0.195
0.075CysMet: 0.075 ± 0.072
0.673CysAsn: 0.673 ± 0.255
0.673CysPro: 0.673 ± 0.225
0.0CysGln: 0.0 ± 0.0
1.047CysArg: 1.047 ± 0.361
0.898CysSer: 0.898 ± 0.23
0.374CysThr: 0.374 ± 0.209
0.823CysVal: 0.823 ± 0.243
0.598CysTrp: 0.598 ± 0.251
0.673CysTyr: 0.673 ± 0.192
0.0CysXaa: 0.0 ± 0.0
Asp
8.528AspAla: 8.528 ± 1.147
0.598AspCys: 0.598 ± 0.213
3.741AspAsp: 3.741 ± 0.554
4.04AspGlu: 4.04 ± 0.514
2.319AspPhe: 2.319 ± 0.436
5.835AspGly: 5.835 ± 0.806
0.898AspHis: 0.898 ± 0.285
3.741AspIle: 3.741 ± 0.503
2.469AspLys: 2.469 ± 0.472
3.591AspLeu: 3.591 ± 0.665
1.646AspMet: 1.646 ± 0.323
2.544AspAsn: 2.544 ± 0.493
1.571AspPro: 1.571 ± 0.462
1.721AspGln: 1.721 ± 0.335
3.366AspArg: 3.366 ± 0.449
3.591AspSer: 3.591 ± 0.498
3.366AspThr: 3.366 ± 0.461
3.516AspVal: 3.516 ± 0.515
0.524AspTrp: 0.524 ± 0.19
1.721AspTyr: 1.721 ± 0.37
0.0AspXaa: 0.0 ± 0.0
Glu
6.583GluAla: 6.583 ± 0.831
1.047GluCys: 1.047 ± 0.307
2.394GluAsp: 2.394 ± 0.359
3.591GluGlu: 3.591 ± 0.694
2.843GluPhe: 2.843 ± 0.49
3.965GluGly: 3.965 ± 0.464
1.421GluHis: 1.421 ± 0.37
3.292GluIle: 3.292 ± 0.642
3.516GluLys: 3.516 ± 0.558
6.957GluLeu: 6.957 ± 0.726
2.843GluMet: 2.843 ± 0.466
2.095GluAsn: 2.095 ± 0.381
1.87GluPro: 1.87 ± 0.307
2.768GluGln: 2.768 ± 0.728
4.788GluArg: 4.788 ± 0.76
4.115GluSer: 4.115 ± 0.689
2.693GluThr: 2.693 ± 0.465
4.863GluVal: 4.863 ± 0.702
1.047GluTrp: 1.047 ± 0.229
2.095GluTyr: 2.095 ± 0.407
0.0GluXaa: 0.0 ± 0.0
Phe
2.618PheAla: 2.618 ± 0.496
0.374PheCys: 0.374 ± 0.177
2.992PheAsp: 2.992 ± 0.565
1.87PheGlu: 1.87 ± 0.406
0.823PhePhe: 0.823 ± 0.254
2.394PheGly: 2.394 ± 0.443
0.224PheHis: 0.224 ± 0.129
2.244PheIle: 2.244 ± 0.489
1.571PheLys: 1.571 ± 0.313
1.496PheLeu: 1.496 ± 0.349
0.748PheMet: 0.748 ± 0.264
2.768PheAsn: 2.768 ± 0.585
1.197PhePro: 1.197 ± 0.351
0.973PheGln: 0.973 ± 0.256
1.272PheArg: 1.272 ± 0.295
2.693PheSer: 2.693 ± 0.607
2.618PheThr: 2.618 ± 0.531
2.095PheVal: 2.095 ± 0.471
0.449PheTrp: 0.449 ± 0.189
0.823PheTyr: 0.823 ± 0.221
0.0PheXaa: 0.0 ± 0.0
Gly
7.481GlyAla: 7.481 ± 1.047
1.197GlyCys: 1.197 ± 0.304
4.264GlyAsp: 4.264 ± 0.615
4.863GlyGlu: 4.863 ± 0.642
3.292GlyPhe: 3.292 ± 0.545
6.583GlyGly: 6.583 ± 1.138
1.272GlyHis: 1.272 ± 0.327
3.142GlyIle: 3.142 ± 0.619
5.012GlyLys: 5.012 ± 0.987
4.264GlyLeu: 4.264 ± 0.503
2.095GlyMet: 2.095 ± 0.397
3.666GlyAsn: 3.666 ± 0.766
1.197GlyPro: 1.197 ± 0.315
2.394GlyGln: 2.394 ± 0.418
3.666GlyArg: 3.666 ± 0.492
4.563GlySer: 4.563 ± 0.672
3.965GlyThr: 3.965 ± 0.602
6.284GlyVal: 6.284 ± 0.629
1.272GlyTrp: 1.272 ± 0.302
2.17GlyTyr: 2.17 ± 0.475
0.0GlyXaa: 0.0 ± 0.0
His
1.347HisAla: 1.347 ± 0.411
0.449HisCys: 0.449 ± 0.171
1.646HisAsp: 1.646 ± 0.316
1.122HisGlu: 1.122 ± 0.246
0.224HisPhe: 0.224 ± 0.119
1.197HisGly: 1.197 ± 0.299
0.673HisHis: 0.673 ± 0.221
1.197HisIle: 1.197 ± 0.28
1.272HisLys: 1.272 ± 0.346
0.823HisLeu: 0.823 ± 0.242
0.598HisMet: 0.598 ± 0.194
0.449HisAsn: 0.449 ± 0.177
0.449HisPro: 0.449 ± 0.193
0.748HisGln: 0.748 ± 0.203
0.823HisArg: 0.823 ± 0.229
0.898HisSer: 0.898 ± 0.214
0.898HisThr: 0.898 ± 0.27
1.122HisVal: 1.122 ± 0.248
0.15HisTrp: 0.15 ± 0.123
1.122HisTyr: 1.122 ± 0.349
0.0HisXaa: 0.0 ± 0.0
Ile
6.284IleAla: 6.284 ± 0.913
0.673IleCys: 0.673 ± 0.182
4.713IleAsp: 4.713 ± 0.514
3.366IleGlu: 3.366 ± 0.432
1.795IlePhe: 1.795 ± 0.427
3.89IleGly: 3.89 ± 0.659
0.823IleHis: 0.823 ± 0.232
4.115IleIle: 4.115 ± 0.54
3.366IleLys: 3.366 ± 0.611
3.366IleLeu: 3.366 ± 0.533
0.973IleMet: 0.973 ± 0.232
3.067IleAsn: 3.067 ± 0.52
2.319IlePro: 2.319 ± 0.367
2.544IleGln: 2.544 ± 0.53
2.544IleArg: 2.544 ± 0.494
4.489IleSer: 4.489 ± 0.578
4.264IleThr: 4.264 ± 0.557
4.414IleVal: 4.414 ± 0.476
0.299IleTrp: 0.299 ± 0.182
1.795IleTyr: 1.795 ± 0.402
0.0IleXaa: 0.0 ± 0.0
Lys
7.182LysAla: 7.182 ± 1.207
0.973LysCys: 0.973 ± 0.239
2.02LysAsp: 2.02 ± 0.441
4.115LysGlu: 4.115 ± 0.624
1.571LysPhe: 1.571 ± 0.293
2.544LysGly: 2.544 ± 0.331
0.823LysHis: 0.823 ± 0.223
3.142LysIle: 3.142 ± 0.603
4.115LysLys: 4.115 ± 0.619
5.312LysLeu: 5.312 ± 0.606
2.918LysMet: 2.918 ± 0.639
1.795LysAsn: 1.795 ± 0.302
2.17LysPro: 2.17 ± 0.376
2.843LysGln: 2.843 ± 0.467
3.366LysArg: 3.366 ± 0.384
3.292LysSer: 3.292 ± 0.481
2.992LysThr: 2.992 ± 0.424
3.292LysVal: 3.292 ± 0.423
0.823LysTrp: 0.823 ± 0.241
1.87LysTyr: 1.87 ± 0.284
0.0LysXaa: 0.0 ± 0.0
Leu
7.93LeuAla: 7.93 ± 0.939
0.898LeuCys: 0.898 ± 0.263
3.292LeuAsp: 3.292 ± 0.553
4.414LeuGlu: 4.414 ± 0.652
1.646LeuPhe: 1.646 ± 0.365
3.441LeuGly: 3.441 ± 0.479
1.122LeuHis: 1.122 ± 0.279
4.788LeuIle: 4.788 ± 0.676
5.012LeuLys: 5.012 ± 0.585
5.76LeuLeu: 5.76 ± 0.636
1.87LeuMet: 1.87 ± 0.432
3.666LeuAsn: 3.666 ± 0.511
3.067LeuPro: 3.067 ± 0.486
2.992LeuGln: 2.992 ± 0.5
4.638LeuArg: 4.638 ± 0.572
6.135LeuSer: 6.135 ± 0.679
5.386LeuThr: 5.386 ± 0.625
4.414LeuVal: 4.414 ± 0.536
0.973LeuTrp: 0.973 ± 0.342
2.02LeuTyr: 2.02 ± 0.339
0.0LeuXaa: 0.0 ± 0.0
Met
3.292MetAla: 3.292 ± 0.472
0.075MetCys: 0.075 ± 0.074
0.973MetAsp: 0.973 ± 0.211
1.421MetGlu: 1.421 ± 0.355
0.598MetPhe: 0.598 ± 0.202
1.571MetGly: 1.571 ± 0.391
0.374MetHis: 0.374 ± 0.163
1.646MetIle: 1.646 ± 0.447
2.02MetLys: 2.02 ± 0.443
2.693MetLeu: 2.693 ± 0.509
0.673MetMet: 0.673 ± 0.261
1.272MetAsn: 1.272 ± 0.304
1.421MetPro: 1.421 ± 0.353
0.898MetGln: 0.898 ± 0.242
1.945MetArg: 1.945 ± 0.398
2.02MetSer: 2.02 ± 0.449
2.394MetThr: 2.394 ± 0.367
1.646MetVal: 1.646 ± 0.32
0.524MetTrp: 0.524 ± 0.21
0.673MetTyr: 0.673 ± 0.214
0.0MetXaa: 0.0 ± 0.0
Asn
5.386AsnAla: 5.386 ± 0.707
0.374AsnCys: 0.374 ± 0.168
2.992AsnAsp: 2.992 ± 0.392
2.992AsnGlu: 2.992 ± 0.412
1.272AsnPhe: 1.272 ± 0.272
3.965AsnGly: 3.965 ± 0.559
0.898AsnHis: 0.898 ± 0.23
2.992AsnIle: 2.992 ± 0.567
2.394AsnLys: 2.394 ± 0.438
2.244AsnLeu: 2.244 ± 0.454
0.598AsnMet: 0.598 ± 0.153
2.768AsnAsn: 2.768 ± 0.694
2.693AsnPro: 2.693 ± 0.429
1.272AsnGln: 1.272 ± 0.373
1.795AsnArg: 1.795 ± 0.424
2.843AsnSer: 2.843 ± 0.389
2.319AsnThr: 2.319 ± 0.446
3.366AsnVal: 3.366 ± 0.45
0.748AsnTrp: 0.748 ± 0.261
1.197AsnTyr: 1.197 ± 0.247
0.0AsnXaa: 0.0 ± 0.0
Pro
4.04ProAla: 4.04 ± 0.716
0.748ProCys: 0.748 ± 0.274
2.469ProAsp: 2.469 ± 0.471
2.244ProGlu: 2.244 ± 0.357
1.87ProPhe: 1.87 ± 0.449
2.319ProGly: 2.319 ± 0.469
0.748ProHis: 0.748 ± 0.271
1.87ProIle: 1.87 ± 0.355
1.571ProLys: 1.571 ± 0.266
2.768ProLeu: 2.768 ± 0.56
1.047ProMet: 1.047 ± 0.274
2.095ProAsn: 2.095 ± 0.577
0.898ProPro: 0.898 ± 0.263
1.496ProGln: 1.496 ± 0.321
1.197ProArg: 1.197 ± 0.333
2.095ProSer: 2.095 ± 0.465
1.571ProThr: 1.571 ± 0.319
3.217ProVal: 3.217 ± 0.552
0.374ProTrp: 0.374 ± 0.194
1.421ProTyr: 1.421 ± 0.459
0.0ProXaa: 0.0 ± 0.0
Gln
5.087GlnAla: 5.087 ± 0.75
0.449GlnCys: 0.449 ± 0.19
2.244GlnAsp: 2.244 ± 0.486
2.544GlnGlu: 2.544 ± 0.326
1.272GlnPhe: 1.272 ± 0.266
2.02GlnGly: 2.02 ± 0.349
0.673GlnHis: 0.673 ± 0.221
1.87GlnIle: 1.87 ± 0.419
2.17GlnLys: 2.17 ± 0.461
3.292GlnLeu: 3.292 ± 0.577
1.122GlnMet: 1.122 ± 0.35
1.571GlnAsn: 1.571 ± 0.378
1.496GlnPro: 1.496 ± 0.297
3.89GlnGln: 3.89 ± 0.993
1.945GlnArg: 1.945 ± 0.545
2.469GlnSer: 2.469 ± 0.452
1.646GlnThr: 1.646 ± 0.353
3.217GlnVal: 3.217 ± 0.495
0.449GlnTrp: 0.449 ± 0.206
1.197GlnTyr: 1.197 ± 0.322
0.0GlnXaa: 0.0 ± 0.0
Arg
5.012ArgAla: 5.012 ± 0.561
0.299ArgCys: 0.299 ± 0.181
2.843ArgAsp: 2.843 ± 0.414
4.264ArgGlu: 4.264 ± 0.68
1.496ArgPhe: 1.496 ± 0.283
3.142ArgGly: 3.142 ± 0.509
1.272ArgHis: 1.272 ± 0.34
4.115ArgIle: 4.115 ± 0.504
3.366ArgLys: 3.366 ± 0.539
5.087ArgLeu: 5.087 ± 0.545
1.571ArgMet: 1.571 ± 0.352
2.693ArgAsn: 2.693 ± 0.356
1.646ArgPro: 1.646 ± 0.442
2.319ArgGln: 2.319 ± 0.455
3.666ArgArg: 3.666 ± 0.544
2.843ArgSer: 2.843 ± 0.381
2.02ArgThr: 2.02 ± 0.465
4.638ArgVal: 4.638 ± 0.594
0.449ArgTrp: 0.449 ± 0.184
1.421ArgTyr: 1.421 ± 0.344
0.0ArgXaa: 0.0 ± 0.0
Ser
7.257SerAla: 7.257 ± 0.83
0.973SerCys: 0.973 ± 0.309
4.189SerAsp: 4.189 ± 0.574
2.918SerGlu: 2.918 ± 0.578
2.843SerPhe: 2.843 ± 0.512
6.658SerGly: 6.658 ± 0.793
0.973SerHis: 0.973 ± 0.251
3.741SerIle: 3.741 ± 0.567
3.142SerLys: 3.142 ± 0.44
4.788SerLeu: 4.788 ± 0.443
1.795SerMet: 1.795 ± 0.296
3.142SerAsn: 3.142 ± 0.618
2.693SerPro: 2.693 ± 0.415
2.244SerGln: 2.244 ± 0.372
3.366SerArg: 3.366 ± 0.435
4.563SerSer: 4.563 ± 0.868
3.741SerThr: 3.741 ± 0.621
5.536SerVal: 5.536 ± 0.906
0.673SerTrp: 0.673 ± 0.286
1.646SerTyr: 1.646 ± 0.35
0.0SerXaa: 0.0 ± 0.0
Thr
6.658ThrAla: 6.658 ± 0.871
1.047ThrCys: 1.047 ± 0.282
3.815ThrAsp: 3.815 ± 0.519
4.414ThrGlu: 4.414 ± 0.674
2.02ThrPhe: 2.02 ± 0.51
6.209ThrGly: 6.209 ± 1.158
0.449ThrHis: 0.449 ± 0.151
3.292ThrIle: 3.292 ± 0.531
2.469ThrLys: 2.469 ± 0.378
4.638ThrLeu: 4.638 ± 0.562
1.496ThrMet: 1.496 ± 0.309
2.095ThrAsn: 2.095 ± 0.45
3.142ThrPro: 3.142 ± 0.579
1.721ThrGln: 1.721 ± 0.331
1.945ThrArg: 1.945 ± 0.318
3.441ThrSer: 3.441 ± 0.501
4.115ThrThr: 4.115 ± 0.545
3.366ThrVal: 3.366 ± 0.76
0.598ThrTrp: 0.598 ± 0.175
1.87ThrTyr: 1.87 ± 0.374
0.0ThrXaa: 0.0 ± 0.0
Val
5.985ValAla: 5.985 ± 0.597
0.823ValCys: 0.823 ± 0.229
5.012ValAsp: 5.012 ± 0.675
4.788ValGlu: 4.788 ± 0.567
1.421ValPhe: 1.421 ± 0.353
4.788ValGly: 4.788 ± 0.718
0.823ValHis: 0.823 ± 0.217
4.115ValIle: 4.115 ± 0.594
4.04ValLys: 4.04 ± 0.508
3.741ValLeu: 3.741 ± 0.49
2.02ValMet: 2.02 ± 0.395
3.217ValAsn: 3.217 ± 0.615
2.992ValPro: 2.992 ± 0.501
3.292ValGln: 3.292 ± 0.519
3.89ValArg: 3.89 ± 0.683
6.06ValSer: 6.06 ± 1.031
5.162ValThr: 5.162 ± 1.004
4.638ValVal: 4.638 ± 0.76
0.973ValTrp: 0.973 ± 0.264
2.319ValTyr: 2.319 ± 0.442
0.0ValXaa: 0.0 ± 0.0
Trp
0.823TrpAla: 0.823 ± 0.288
0.075TrpCys: 0.075 ± 0.083
0.598TrpAsp: 0.598 ± 0.179
0.673TrpGlu: 0.673 ± 0.222
0.299TrpPhe: 0.299 ± 0.16
0.598TrpGly: 0.598 ± 0.215
0.598TrpHis: 0.598 ± 0.257
0.673TrpIle: 0.673 ± 0.264
1.047TrpLys: 1.047 ± 0.308
1.047TrpLeu: 1.047 ± 0.251
0.299TrpMet: 0.299 ± 0.136
0.524TrpAsn: 0.524 ± 0.193
0.524TrpPro: 0.524 ± 0.22
0.748TrpGln: 0.748 ± 0.296
0.898TrpArg: 0.898 ± 0.366
0.748TrpSer: 0.748 ± 0.271
0.598TrpThr: 0.598 ± 0.221
1.646TrpVal: 1.646 ± 0.466
0.075TrpTrp: 0.075 ± 0.084
0.374TrpTyr: 0.374 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.095TyrAla: 2.095 ± 0.399
0.449TyrCys: 0.449 ± 0.169
2.17TyrAsp: 2.17 ± 0.458
1.721TyrGlu: 1.721 ± 0.394
0.973TyrPhe: 0.973 ± 0.227
2.544TyrGly: 2.544 ± 0.389
0.748TyrHis: 0.748 ± 0.195
1.496TyrIle: 1.496 ± 0.345
1.721TyrLys: 1.721 ± 0.378
2.618TyrLeu: 2.618 ± 0.477
0.598TyrMet: 0.598 ± 0.183
1.197TyrAsn: 1.197 ± 0.241
1.421TyrPro: 1.421 ± 0.488
1.421TyrGln: 1.421 ± 0.337
2.618TyrArg: 2.618 ± 0.437
2.693TyrSer: 2.693 ± 0.471
1.721TyrThr: 1.721 ± 0.507
1.496TyrVal: 1.496 ± 0.389
0.374TyrTrp: 0.374 ± 0.166
1.047TyrTyr: 1.047 ± 0.257
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (13368 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski