Amino acid dipepetide frequency for Streptomyces sp. NRRL S-1521

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.174AlaAla: 22.174 ± 0.17	1.14AlaCys: 1.14 ± 0.023	8.852AlaAsp: 8.852 ± 0.064	9.363AlaGlu: 9.363 ± 0.092	3.591AlaPhe: 3.591 ± 0.045	13.4AlaGly: 13.4 ± 0.104	3.079AlaHis: 3.079 ± 0.043	3.366AlaIle: 3.366 ± 0.041	3.049AlaLys: 3.049 ± 0.058	14.951AlaLeu: 14.951 ± 0.125	2.56AlaMet: 2.56 ± 0.033	1.78AlaAsn: 1.78 ± 0.027	7.543AlaPro: 7.543 ± 0.075	3.551AlaGln: 3.551 ± 0.049	10.85AlaArg: 10.85 ± 0.107	5.944AlaSer: 5.944 ± 0.058	6.866AlaThr: 6.866 ± 0.054	12.338AlaVal: 12.338 ± 0.084	1.87AlaTrp: 1.87 ± 0.029	2.811AlaTyr: 2.811 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	1.162CysAla: 1.162 ± 0.022	0.089CysCys: 0.089 ± 0.006	0.459CysAsp: 0.459 ± 0.015	0.437CysGlu: 0.437 ± 0.014	0.217CysPhe: 0.217 ± 0.011	0.983CysGly: 0.983 ± 0.02	0.188CysHis: 0.188 ± 0.01	0.139CysIle: 0.139 ± 0.008	0.111CysLys: 0.111 ± 0.006	0.774CysLeu: 0.774 ± 0.02	0.131CysMet: 0.131 ± 0.008	0.121CysAsn: 0.121 ± 0.007	0.492CysPro: 0.492 ± 0.014	0.157CysGln: 0.157 ± 0.008	0.624CysArg: 0.624 ± 0.018	0.413CysSer: 0.413 ± 0.014	0.471CysThr: 0.471 ± 0.015	0.709CysVal: 0.709 ± 0.017	0.124CysTrp: 0.124 ± 0.008	0.141CysTyr: 0.141 ± 0.006	0.0CysXaa: 0.0 ± 0.0
Asp	8.314AspAla: 8.314 ± 0.07	0.435AspCys: 0.435 ± 0.016	3.888AspAsp: 3.888 ± 0.05	4.067AspGlu: 4.067 ± 0.043	1.68AspPhe: 1.68 ± 0.028	6.522AspGly: 6.522 ± 0.063	1.489AspHis: 1.489 ± 0.025	1.918AspIle: 1.918 ± 0.034	1.359AspLys: 1.359 ± 0.031	6.267AspLeu: 6.267 ± 0.058	0.874AspMet: 0.874 ± 0.02	0.868AspAsn: 0.868 ± 0.021	4.512AspPro: 4.512 ± 0.053	1.448AspGln: 1.448 ± 0.026	4.916AspArg: 4.916 ± 0.047	2.483AspSer: 2.483 ± 0.031	3.159AspThr: 3.159 ± 0.041	4.911AspVal: 4.911 ± 0.047	1.001AspTrp: 1.001 ± 0.024	1.034AspTyr: 1.034 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	7.874GluAla: 7.874 ± 0.076	0.366GluCys: 0.366 ± 0.012	2.921GluAsp: 2.921 ± 0.041	3.652GluGlu: 3.652 ± 0.048	1.474GluPhe: 1.474 ± 0.023	4.634GluGly: 4.634 ± 0.048	1.58GluHis: 1.58 ± 0.026	2.164GluIle: 2.164 ± 0.034	1.591GluLys: 1.591 ± 0.034	6.893GluLeu: 6.893 ± 0.064	0.927GluMet: 0.927 ± 0.022	1.012GluAsn: 1.012 ± 0.027	3.531GluPro: 3.531 ± 0.043	2.261GluGln: 2.261 ± 0.04	5.967GluArg: 5.967 ± 0.05	2.56GluSer: 2.56 ± 0.035	2.925GluThr: 2.925 ± 0.04	4.397GluVal: 4.397 ± 0.045	0.744GluTrp: 0.744 ± 0.021	1.112GluTyr: 1.112 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.776PheAla: 3.776 ± 0.043	0.288PheCys: 0.288 ± 0.01	2.011PheAsp: 2.011 ± 0.03	1.412PheGlu: 1.412 ± 0.028	0.869PhePhe: 0.869 ± 0.025	3.01PheGly: 3.01 ± 0.041	0.625PheHis: 0.625 ± 0.017	0.743PheIle: 0.743 ± 0.017	0.502PheLys: 0.502 ± 0.019	2.592PheLeu: 2.592 ± 0.033	0.417PheMet: 0.417 ± 0.012	0.538PheAsn: 0.538 ± 0.017	1.352PhePro: 1.352 ± 0.029	0.612PheGln: 0.612 ± 0.016	1.848PheArg: 1.848 ± 0.029	1.456PheSer: 1.456 ± 0.024	1.991PheThr: 1.991 ± 0.037	2.161PheVal: 2.161 ± 0.032	0.412PheTrp: 0.412 ± 0.017	0.555PheTyr: 0.555 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	11.858GlyAla: 11.858 ± 0.082	0.807GlyCys: 0.807 ± 0.02	5.498GlyAsp: 5.498 ± 0.056	5.504GlyGlu: 5.504 ± 0.061	2.868GlyPhe: 2.868 ± 0.038	9.209GlyGly: 9.209 ± 0.098	2.437GlyHis: 2.437 ± 0.033	3.245GlyIle: 3.245 ± 0.038	2.604GlyLys: 2.604 ± 0.05	9.467GlyLeu: 9.467 ± 0.07	2.01GlyMet: 2.01 ± 0.031	1.624GlyAsn: 1.624 ± 0.034	5.365GlyPro: 5.365 ± 0.065	2.557GlyGln: 2.557 ± 0.033	8.022GlyArg: 8.022 ± 0.068	5.235GlySer: 5.235 ± 0.055	5.664GlyThr: 5.664 ± 0.064	7.865GlyVal: 7.865 ± 0.07	1.609GlyTrp: 1.609 ± 0.031	2.219GlyTyr: 2.219 ± 0.032	0.0GlyXaa: 0.0 ± 0.0
His	2.849HisAla: 2.849 ± 0.042	0.235HisCys: 0.235 ± 0.01	1.382HisAsp: 1.382 ± 0.025	1.301HisGlu: 1.301 ± 0.025	0.663HisPhe: 0.663 ± 0.018	2.506HisGly: 2.506 ± 0.027	0.728HisHis: 0.728 ± 0.018	0.738HisIle: 0.738 ± 0.018	0.383HisLys: 0.383 ± 0.013	2.498HisLeu: 2.498 ± 0.036	0.366HisMet: 0.366 ± 0.014	0.345HisAsn: 0.345 ± 0.015	1.805HisPro: 1.805 ± 0.031	0.647HisGln: 0.647 ± 0.019	2.143HisArg: 2.143 ± 0.034	1.017HisSer: 1.017 ± 0.023	1.391HisThr: 1.391 ± 0.025	1.817HisVal: 1.817 ± 0.03	0.363HisTrp: 0.363 ± 0.013	0.492HisTyr: 0.492 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	4.653IleAla: 4.653 ± 0.054	0.286IleCys: 0.286 ± 0.011	2.177IleAsp: 2.177 ± 0.035	1.969IleGlu: 1.969 ± 0.031	0.669IlePhe: 0.669 ± 0.018	3.599IleGly: 3.599 ± 0.042	0.59IleHis: 0.59 ± 0.018	0.834IleIle: 0.834 ± 0.023	0.757IleLys: 0.757 ± 0.022	2.31IleLeu: 2.31 ± 0.035	0.417IleMet: 0.417 ± 0.015	0.65IleAsn: 0.65 ± 0.02	1.624IlePro: 1.624 ± 0.023	0.649IleGln: 0.649 ± 0.017	2.102IleArg: 2.102 ± 0.033	1.578IleSer: 1.578 ± 0.031	2.092IleThr: 2.092 ± 0.034	2.59IleVal: 2.59 ± 0.04	0.353IleTrp: 0.353 ± 0.013	0.5IleTyr: 0.5 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	3.14LysAla: 3.14 ± 0.051	0.116LysCys: 0.116 ± 0.007	1.471LysAsp: 1.471 ± 0.036	1.324LysGlu: 1.324 ± 0.027	0.456LysPhe: 0.456 ± 0.018	2.011LysGly: 2.011 ± 0.042	0.453LysHis: 0.453 ± 0.016	0.872LysIle: 0.872 ± 0.023	1.054LysLys: 1.054 ± 0.037	2.035LysLeu: 2.035 ± 0.037	0.426LysMet: 0.426 ± 0.015	0.552LysAsn: 0.552 ± 0.021	1.403LysPro: 1.403 ± 0.03	0.699LysGln: 0.699 ± 0.02	1.559LysArg: 1.559 ± 0.03	1.192LysSer: 1.192 ± 0.029	1.328LysThr: 1.328 ± 0.031	1.945LysVal: 1.945 ± 0.036	0.273LysTrp: 0.273 ± 0.011	0.463LysTyr: 0.463 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.414LeuAla: 15.414 ± 0.124	0.871LeuCys: 0.871 ± 0.021	6.884LeuAsp: 6.884 ± 0.066	4.446LeuGlu: 4.446 ± 0.045	2.669LeuPhe: 2.669 ± 0.042	9.321LeuGly: 9.321 ± 0.073	2.338LeuHis: 2.338 ± 0.032	3.232LeuIle: 3.232 ± 0.043	2.154LeuLys: 2.154 ± 0.036	11.396LeuLeu: 11.396 ± 0.108	1.684LeuMet: 1.684 ± 0.026	1.59LeuAsn: 1.59 ± 0.03	6.528LeuPro: 6.528 ± 0.071	2.051LeuGln: 2.051 ± 0.032	8.916LeuArg: 8.916 ± 0.084	5.277LeuSer: 5.277 ± 0.047	6.89LeuThr: 6.89 ± 0.058	8.715LeuVal: 8.715 ± 0.077	1.27LeuTrp: 1.27 ± 0.026	1.771LeuTyr: 1.771 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	2.336MetAla: 2.336 ± 0.034	0.151MetCys: 0.151 ± 0.009	0.936MetAsp: 0.936 ± 0.019	0.795MetGlu: 0.795 ± 0.021	0.468MetPhe: 0.468 ± 0.015	1.348MetGly: 1.348 ± 0.028	0.352MetHis: 0.352 ± 0.012	0.639MetIle: 0.639 ± 0.019	0.444MetLys: 0.444 ± 0.015	1.696MetLeu: 1.696 ± 0.032	0.306MetMet: 0.306 ± 0.014	0.419MetAsn: 0.419 ± 0.014	1.145MetPro: 1.145 ± 0.025	0.409MetGln: 0.409 ± 0.014	1.475MetArg: 1.475 ± 0.023	1.28MetSer: 1.28 ± 0.026	1.509MetThr: 1.509 ± 0.025	1.31MetVal: 1.31 ± 0.026	0.207MetTrp: 0.207 ± 0.01	0.344MetTyr: 0.344 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.034AsnAla: 2.034 ± 0.033	0.157AsnCys: 0.157 ± 0.007	0.916AsnAsp: 0.916 ± 0.022	0.78AsnGlu: 0.78 ± 0.021	0.466AsnPhe: 0.466 ± 0.015	1.707AsnGly: 1.707 ± 0.031	0.409AsnHis: 0.409 ± 0.012	0.587AsnIle: 0.587 ± 0.018	0.411AsnLys: 0.411 ± 0.015	1.564AsnLeu: 1.564 ± 0.032	0.278AsnMet: 0.278 ± 0.011	0.38AsnAsn: 0.38 ± 0.014	1.212AsnPro: 1.212 ± 0.025	0.463AsnGln: 0.463 ± 0.014	1.215AsnArg: 1.215 ± 0.023	0.842AsnSer: 0.842 ± 0.023	1.015AsnThr: 1.015 ± 0.027	1.284AsnVal: 1.284 ± 0.026	0.255AsnTrp: 0.255 ± 0.011	0.397AsnTyr: 0.397 ± 0.012	0.0AsnXaa: 0.0 ± 0.0
Pro	8.878ProAla: 8.878 ± 0.085	0.347ProCys: 0.347 ± 0.013	4.504ProAsp: 4.504 ± 0.052	4.484ProGlu: 4.484 ± 0.049	1.507ProPhe: 1.507 ± 0.027	6.729ProGly: 6.729 ± 0.062	1.475ProHis: 1.475 ± 0.028	1.209ProIle: 1.209 ± 0.024	1.273ProLys: 1.273 ± 0.027	5.515ProLeu: 5.515 ± 0.07	0.993ProMet: 0.993 ± 0.021	0.813ProAsn: 0.813 ± 0.019	3.493ProPro: 3.493 ± 0.063	1.669ProGln: 1.669 ± 0.036	4.213ProArg: 4.213 ± 0.047	3.02ProSer: 3.02 ± 0.038	2.997ProThr: 2.997 ± 0.04	5.25ProVal: 5.25 ± 0.05	0.896ProTrp: 0.896 ± 0.021	1.359ProTyr: 1.359 ± 0.025	0.0ProXaa: 0.0 ± 0.0
Gln	3.433GlnAla: 3.433 ± 0.048	0.17GlnCys: 0.17 ± 0.008	1.357GlnAsp: 1.357 ± 0.026	1.474GlnGlu: 1.474 ± 0.024	0.643GlnPhe: 0.643 ± 0.019	2.327GlnGly: 2.327 ± 0.033	0.639GlnHis: 0.639 ± 0.017	1.007GlnIle: 1.007 ± 0.021	0.616GlnLys: 0.616 ± 0.019	2.802GlnLeu: 2.802 ± 0.037	0.481GlnMet: 0.481 ± 0.016	0.456GlnAsn: 0.456 ± 0.017	1.542GlnPro: 1.542 ± 0.03	1.177GlnGln: 1.177 ± 0.037	2.35GlnArg: 2.35 ± 0.033	1.196GlnSer: 1.196 ± 0.021	1.26GlnThr: 1.26 ± 0.025	2.171GlnVal: 2.171 ± 0.03	0.451GlnTrp: 0.451 ± 0.014	0.59GlnTyr: 0.59 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	10.537ArgAla: 10.537 ± 0.095	0.605ArgCys: 0.605 ± 0.018	4.526ArgAsp: 4.526 ± 0.046	5.051ArgGlu: 5.051 ± 0.057	2.381ArgPhe: 2.381 ± 0.036	6.248ArgGly: 6.248 ± 0.055	2.302ArgHis: 2.302 ± 0.037	2.918ArgIle: 2.918 ± 0.04	1.66ArgLys: 1.66 ± 0.03	9.02ArgLeu: 9.02 ± 0.088	1.787ArgMet: 1.787 ± 0.028	1.239ArgAsn: 1.239 ± 0.022	5.134ArgPro: 5.134 ± 0.058	2.286ArgGln: 2.286 ± 0.035	7.795ArgArg: 7.795 ± 0.066	3.937ArgSer: 3.937 ± 0.042	5.405ArgThr: 5.405 ± 0.059	6.218ArgVal: 6.218 ± 0.057	1.408ArgTrp: 1.408 ± 0.026	1.73ArgTyr: 1.73 ± 0.03	0.0ArgXaa: 0.0 ± 0.0
Ser	6.705SerAla: 6.705 ± 0.059	0.388SerCys: 0.388 ± 0.013	2.618SerAsp: 2.618 ± 0.037	2.371SerGlu: 2.371 ± 0.034	1.492SerPhe: 1.492 ± 0.027	5.832SerGly: 5.832 ± 0.059	1.059SerHis: 1.059 ± 0.022	1.345SerIle: 1.345 ± 0.026	1.062SerLys: 1.062 ± 0.026	4.834SerLeu: 4.834 ± 0.05	1.037SerMet: 1.037 ± 0.021	0.793SerAsn: 0.793 ± 0.02	2.971SerPro: 2.971 ± 0.036	1.138SerGln: 1.138 ± 0.024	3.671SerArg: 3.671 ± 0.046	2.619SerSer: 2.619 ± 0.043	2.85SerThr: 2.85 ± 0.034	4.177SerVal: 4.177 ± 0.049	0.852SerTrp: 0.852 ± 0.02	1.191SerTyr: 1.191 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	8.746ThrAla: 8.746 ± 0.071	0.399ThrCys: 0.399 ± 0.013	3.433ThrAsp: 3.433 ± 0.044	3.252ThrGlu: 3.252 ± 0.038	1.56ThrPhe: 1.56 ± 0.026	6.494ThrGly: 6.494 ± 0.062	1.218ThrHis: 1.218 ± 0.025	1.588ThrIle: 1.588 ± 0.03	1.187ThrLys: 1.187 ± 0.03	5.656ThrLeu: 5.656 ± 0.058	0.924ThrMet: 0.924 ± 0.023	0.901ThrAsn: 0.901 ± 0.022	4.005ThrPro: 4.005 ± 0.048	1.257ThrGln: 1.257 ± 0.023	3.916ThrArg: 3.916 ± 0.041	2.996ThrSer: 2.996 ± 0.037	3.539ThrThr: 3.539 ± 0.049	5.777ThrVal: 5.777 ± 0.058	0.89ThrTrp: 0.89 ± 0.02	1.333ThrTyr: 1.333 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	10.758ValAla: 10.758 ± 0.078	0.762ValCys: 0.762 ± 0.018	5.031ValAsp: 5.031 ± 0.053	4.83ValGlu: 4.83 ± 0.053	2.371ValPhe: 2.371 ± 0.039	6.52ValGly: 6.52 ± 0.062	1.963ValHis: 1.963 ± 0.032	2.9ValIle: 2.9 ± 0.043	1.772ValLys: 1.772 ± 0.032	9.406ValLeu: 9.406 ± 0.069	1.418ValMet: 1.418 ± 0.027	1.552ValAsn: 1.552 ± 0.028	5.196ValPro: 5.196 ± 0.046	1.938ValGln: 1.938 ± 0.028	7.415ValArg: 7.415 ± 0.062	4.19ValSer: 4.19 ± 0.046	5.612ValThr: 5.612 ± 0.055	7.805ValVal: 7.805 ± 0.074	1.127ValTrp: 1.127 ± 0.021	1.535ValTyr: 1.535 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.637TrpAla: 1.637 ± 0.029	0.157TrpCys: 0.157 ± 0.007	0.807TrpAsp: 0.807 ± 0.02	0.743TrpGlu: 0.743 ± 0.019	0.496TrpPhe: 0.496 ± 0.014	1.029TrpGly: 1.029 ± 0.021	0.364TrpHis: 0.364 ± 0.011	0.507TrpIle: 0.507 ± 0.016	0.374TrpLys: 0.374 ± 0.013	1.738TrpLeu: 1.738 ± 0.031	0.289TrpMet: 0.289 ± 0.012	0.372TrpAsn: 0.372 ± 0.013	0.762TrpPro: 0.762 ± 0.022	0.612TrpGln: 0.612 ± 0.017	1.406TrpArg: 1.406 ± 0.026	0.88TrpSer: 0.88 ± 0.023	1.004TrpThr: 1.004 ± 0.021	0.933TrpVal: 0.933 ± 0.022	0.303TrpTrp: 0.303 ± 0.011	0.333TrpTyr: 0.333 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.8TyrAla: 2.8 ± 0.04	0.172TyrCys: 0.172 ± 0.01	1.475TyrAsp: 1.475 ± 0.031	1.33TyrGlu: 1.33 ± 0.026	0.63TyrPhe: 0.63 ± 0.015	2.24TyrGly: 2.24 ± 0.035	0.381TyrHis: 0.381 ± 0.013	0.448TyrIle: 0.448 ± 0.016	0.41TyrLys: 0.41 ± 0.015	2.002TyrLeu: 2.002 ± 0.03	0.245TyrMet: 0.245 ± 0.01	0.369TyrAsn: 0.369 ± 0.014	1.009TyrPro: 1.009 ± 0.022	0.558TyrGln: 0.558 ± 0.015	1.782TyrArg: 1.782 ± 0.031	0.888TyrSer: 0.888 ± 0.021	1.102TyrThr: 1.102 ± 0.026	1.729TyrVal: 1.729 ± 0.028	0.341TyrTrp: 0.341 ± 0.014	0.426TyrTyr: 0.426 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6869 proteins (2333221 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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