Amino acid dipepetide frequency for Ralstonia phage Simangalove

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.981AlaAla: 17.981 ± 1.433
1.058AlaCys: 1.058 ± 0.346
7.026AlaAsp: 7.026 ± 0.741
7.404AlaGlu: 7.404 ± 0.822
4.231AlaPhe: 4.231 ± 0.521
11.484AlaGly: 11.484 ± 1.359
1.587AlaHis: 1.587 ± 0.406
5.138AlaIle: 5.138 ± 0.604
4.08AlaLys: 4.08 ± 0.638
11.408AlaLeu: 11.408 ± 1.026
3.626AlaMet: 3.626 ± 0.465
5.138AlaAsn: 5.138 ± 0.665
6.8AlaPro: 6.8 ± 0.8
7.026AlaGln: 7.026 ± 0.788
9.066AlaArg: 9.066 ± 0.889
7.177AlaSer: 7.177 ± 0.815
7.857AlaThr: 7.857 ± 1.114
7.328AlaVal: 7.328 ± 0.693
1.133AlaTrp: 1.133 ± 0.331
3.173AlaTyr: 3.173 ± 0.5
0.0AlaXaa: 0.0 ± 0.0
Cys
0.907CysAla: 0.907 ± 0.256
0.302CysCys: 0.302 ± 0.173
0.907CysAsp: 0.907 ± 0.266
0.453CysGlu: 0.453 ± 0.168
0.076CysPhe: 0.076 ± 0.084
0.907CysGly: 0.907 ± 0.278
0.302CysHis: 0.302 ± 0.164
0.453CysIle: 0.453 ± 0.176
0.453CysLys: 0.453 ± 0.174
0.529CysLeu: 0.529 ± 0.241
0.0CysMet: 0.0 ± 0.0
0.453CysAsn: 0.453 ± 0.161
0.378CysPro: 0.378 ± 0.209
0.227CysGln: 0.227 ± 0.162
1.058CysArg: 1.058 ± 0.337
0.453CysSer: 0.453 ± 0.165
0.831CysThr: 0.831 ± 0.225
0.982CysVal: 0.982 ± 0.247
0.0CysTrp: 0.0 ± 0.0
0.302CysTyr: 0.302 ± 0.143
0.0CysXaa: 0.0 ± 0.0
Asp
7.933AspAla: 7.933 ± 0.701
0.604AspCys: 0.604 ± 0.223
3.626AspAsp: 3.626 ± 0.633
3.702AspGlu: 3.702 ± 0.534
1.813AspPhe: 1.813 ± 0.282
4.684AspGly: 4.684 ± 0.633
0.453AspHis: 0.453 ± 0.177
2.569AspIle: 2.569 ± 0.476
1.813AspLys: 1.813 ± 0.387
4.533AspLeu: 4.533 ± 0.483
1.738AspMet: 1.738 ± 0.462
1.964AspAsn: 1.964 ± 0.399
4.155AspPro: 4.155 ± 0.669
1.511AspGln: 1.511 ± 0.355
2.342AspArg: 2.342 ± 0.448
3.022AspSer: 3.022 ± 0.455
3.4AspThr: 3.4 ± 0.472
3.853AspVal: 3.853 ± 0.443
1.209AspTrp: 1.209 ± 0.24
1.435AspTyr: 1.435 ± 0.315
0.0AspXaa: 0.0 ± 0.0
Glu
6.649GluAla: 6.649 ± 0.828
0.529GluCys: 0.529 ± 0.217
2.04GluAsp: 2.04 ± 0.465
3.324GluGlu: 3.324 ± 0.51
1.36GluPhe: 1.36 ± 0.322
3.702GluGly: 3.702 ± 0.479
1.284GluHis: 1.284 ± 0.318
2.191GluIle: 2.191 ± 0.379
2.191GluLys: 2.191 ± 0.422
5.213GluLeu: 5.213 ± 0.517
0.907GluMet: 0.907 ± 0.211
1.889GluAsn: 1.889 ± 0.288
1.738GluPro: 1.738 ± 0.476
3.475GluGln: 3.475 ± 0.392
4.684GluArg: 4.684 ± 0.807
2.493GluSer: 2.493 ± 0.534
2.795GluThr: 2.795 ± 0.496
2.72GluVal: 2.72 ± 0.417
0.453GluTrp: 0.453 ± 0.176
1.284GluTyr: 1.284 ± 0.277
0.0GluXaa: 0.0 ± 0.0
Phe
4.231PheAla: 4.231 ± 0.587
0.529PheCys: 0.529 ± 0.204
2.947PheAsp: 2.947 ± 0.471
1.662PheGlu: 1.662 ± 0.353
1.058PhePhe: 1.058 ± 0.329
2.191PheGly: 2.191 ± 0.425
0.529PheHis: 0.529 ± 0.179
1.133PheIle: 1.133 ± 0.227
0.982PheLys: 0.982 ± 0.269
2.644PheLeu: 2.644 ± 0.49
0.529PheMet: 0.529 ± 0.191
1.587PheAsn: 1.587 ± 0.305
1.662PhePro: 1.662 ± 0.423
0.982PheGln: 0.982 ± 0.296
1.738PheArg: 1.738 ± 0.289
1.964PheSer: 1.964 ± 0.334
2.418PheThr: 2.418 ± 0.486
2.644PheVal: 2.644 ± 0.402
0.378PheTrp: 0.378 ± 0.144
1.133PheTyr: 1.133 ± 0.278
0.0PheXaa: 0.0 ± 0.0
Gly
10.728GlyAla: 10.728 ± 1.328
0.756GlyCys: 0.756 ± 0.225
3.475GlyAsp: 3.475 ± 0.412
4.08GlyGlu: 4.08 ± 0.71
3.929GlyPhe: 3.929 ± 0.506
7.782GlyGly: 7.782 ± 1.901
1.209GlyHis: 1.209 ± 0.291
2.871GlyIle: 2.871 ± 0.443
3.249GlyLys: 3.249 ± 0.45
6.422GlyLeu: 6.422 ± 0.663
1.133GlyMet: 1.133 ± 0.304
2.795GlyAsn: 2.795 ± 0.41
2.72GlyPro: 2.72 ± 0.515
2.569GlyGln: 2.569 ± 0.54
4.76GlyArg: 4.76 ± 0.574
4.458GlySer: 4.458 ± 0.969
5.742GlyThr: 5.742 ± 0.93
7.102GlyVal: 7.102 ± 0.65
1.511GlyTrp: 1.511 ± 0.3
3.098GlyTyr: 3.098 ± 0.549
0.0GlyXaa: 0.0 ± 0.0
His
2.418HisAla: 2.418 ± 0.344
0.227HisCys: 0.227 ± 0.142
1.133HisAsp: 1.133 ± 0.288
1.058HisGlu: 1.058 ± 0.257
0.529HisPhe: 0.529 ± 0.181
1.889HisGly: 1.889 ± 0.509
0.227HisHis: 0.227 ± 0.127
0.831HisIle: 0.831 ± 0.265
0.378HisLys: 0.378 ± 0.16
1.511HisLeu: 1.511 ± 0.357
0.227HisMet: 0.227 ± 0.113
0.907HisAsn: 0.907 ± 0.295
0.68HisPro: 0.68 ± 0.222
0.529HisGln: 0.529 ± 0.178
1.36HisArg: 1.36 ± 0.347
1.133HisSer: 1.133 ± 0.268
0.604HisThr: 0.604 ± 0.246
1.435HisVal: 1.435 ± 0.368
0.076HisTrp: 0.076 ± 0.074
0.453HisTyr: 0.453 ± 0.217
0.0HisXaa: 0.0 ± 0.0
Ile
5.515IleAla: 5.515 ± 0.658
0.151IleCys: 0.151 ± 0.101
3.022IleAsp: 3.022 ± 0.406
2.418IleGlu: 2.418 ± 0.464
1.36IlePhe: 1.36 ± 0.348
2.418IleGly: 2.418 ± 0.4
0.756IleHis: 0.756 ± 0.245
1.133IleIle: 1.133 ± 0.304
1.511IleLys: 1.511 ± 0.411
2.569IleLeu: 2.569 ± 0.485
0.982IleMet: 0.982 ± 0.291
1.435IleAsn: 1.435 ± 0.359
2.569IlePro: 2.569 ± 0.387
1.209IleGln: 1.209 ± 0.277
2.569IleArg: 2.569 ± 0.504
2.267IleSer: 2.267 ± 0.419
3.778IleThr: 3.778 ± 0.623
3.626IleVal: 3.626 ± 0.575
0.982IleTrp: 0.982 ± 0.243
1.058IleTyr: 1.058 ± 0.26
0.0IleXaa: 0.0 ± 0.0
Lys
4.155LysAla: 4.155 ± 0.643
0.151LysCys: 0.151 ± 0.105
1.587LysAsp: 1.587 ± 0.316
1.587LysGlu: 1.587 ± 0.288
1.133LysPhe: 1.133 ± 0.252
1.964LysGly: 1.964 ± 0.413
1.209LysHis: 1.209 ± 0.293
1.662LysIle: 1.662 ± 0.315
1.813LysLys: 1.813 ± 0.487
4.004LysLeu: 4.004 ± 0.487
0.907LysMet: 0.907 ± 0.256
0.907LysAsn: 0.907 ± 0.281
2.267LysPro: 2.267 ± 0.332
1.435LysGln: 1.435 ± 0.278
2.115LysArg: 2.115 ± 0.573
1.889LysSer: 1.889 ± 0.319
2.947LysThr: 2.947 ± 0.457
2.493LysVal: 2.493 ± 0.516
0.982LysTrp: 0.982 ± 0.226
0.907LysTyr: 0.907 ± 0.262
0.0LysXaa: 0.0 ± 0.0
Leu
10.653LeuAla: 10.653 ± 0.951
0.982LeuCys: 0.982 ± 0.323
5.515LeuAsp: 5.515 ± 0.596
3.626LeuGlu: 3.626 ± 0.585
2.115LeuPhe: 2.115 ± 0.426
5.515LeuGly: 5.515 ± 0.522
2.191LeuHis: 2.191 ± 0.529
3.098LeuIle: 3.098 ± 0.47
2.569LeuLys: 2.569 ± 0.37
6.724LeuLeu: 6.724 ± 0.633
1.813LeuMet: 1.813 ± 0.327
3.324LeuAsn: 3.324 ± 0.572
5.591LeuPro: 5.591 ± 0.539
3.626LeuGln: 3.626 ± 0.608
6.271LeuArg: 6.271 ± 0.905
5.289LeuSer: 5.289 ± 0.471
5.44LeuThr: 5.44 ± 0.762
6.271LeuVal: 6.271 ± 0.762
1.36LeuTrp: 1.36 ± 0.4
1.964LeuTyr: 1.964 ± 0.458
0.0LeuXaa: 0.0 ± 0.0
Met
3.173MetAla: 3.173 ± 0.503
0.0MetCys: 0.0 ± 0.0
0.907MetAsp: 0.907 ± 0.237
0.529MetGlu: 0.529 ± 0.237
0.378MetPhe: 0.378 ± 0.183
1.36MetGly: 1.36 ± 0.31
0.378MetHis: 0.378 ± 0.149
0.907MetIle: 0.907 ± 0.236
1.209MetLys: 1.209 ± 0.35
1.738MetLeu: 1.738 ± 0.373
0.453MetMet: 0.453 ± 0.197
0.529MetAsn: 0.529 ± 0.209
1.738MetPro: 1.738 ± 0.305
1.209MetGln: 1.209 ± 0.326
2.04MetArg: 2.04 ± 0.373
2.569MetSer: 2.569 ± 0.418
2.115MetThr: 2.115 ± 0.353
0.756MetVal: 0.756 ± 0.236
0.378MetTrp: 0.378 ± 0.171
0.151MetTyr: 0.151 ± 0.081
0.0MetXaa: 0.0 ± 0.0
Asn
5.213AsnAla: 5.213 ± 0.611
0.076AsnCys: 0.076 ± 0.076
1.889AsnAsp: 1.889 ± 0.344
1.738AsnGlu: 1.738 ± 0.327
1.284AsnPhe: 1.284 ± 0.291
2.72AsnGly: 2.72 ± 0.455
0.378AsnHis: 0.378 ± 0.152
1.738AsnIle: 1.738 ± 0.293
1.587AsnLys: 1.587 ± 0.319
2.342AsnLeu: 2.342 ± 0.37
1.284AsnMet: 1.284 ± 0.272
1.587AsnAsn: 1.587 ± 0.413
3.022AsnPro: 3.022 ± 0.464
0.756AsnGln: 0.756 ± 0.227
1.889AsnArg: 1.889 ± 0.339
2.342AsnSer: 2.342 ± 0.444
1.964AsnThr: 1.964 ± 0.35
2.644AsnVal: 2.644 ± 0.514
0.831AsnTrp: 0.831 ± 0.206
1.813AsnTyr: 1.813 ± 0.38
0.0AsnXaa: 0.0 ± 0.0
Pro
7.857ProAla: 7.857 ± 0.772
0.529ProCys: 0.529 ± 0.247
2.72ProAsp: 2.72 ± 0.389
3.249ProGlu: 3.249 ± 0.569
1.662ProPhe: 1.662 ± 0.35
5.817ProGly: 5.817 ± 0.52
0.756ProHis: 0.756 ± 0.271
2.04ProIle: 2.04 ± 0.39
2.493ProLys: 2.493 ± 0.479
4.08ProLeu: 4.08 ± 0.608
0.756ProMet: 0.756 ± 0.214
1.889ProAsn: 1.889 ± 0.315
3.249ProPro: 3.249 ± 0.559
2.342ProGln: 2.342 ± 0.387
2.04ProArg: 2.04 ± 0.331
3.173ProSer: 3.173 ± 0.504
5.364ProThr: 5.364 ± 0.797
3.929ProVal: 3.929 ± 0.452
0.604ProTrp: 0.604 ± 0.212
1.587ProTyr: 1.587 ± 0.324
0.0ProXaa: 0.0 ± 0.0
Gln
5.893GlnAla: 5.893 ± 0.598
0.151GlnCys: 0.151 ± 0.098
2.115GlnAsp: 2.115 ± 0.387
1.738GlnGlu: 1.738 ± 0.397
1.209GlnPhe: 1.209 ± 0.26
2.947GlnGly: 2.947 ± 0.407
1.058GlnHis: 1.058 ± 0.285
3.249GlnIle: 3.249 ± 0.476
1.587GlnLys: 1.587 ± 0.329
3.324GlnLeu: 3.324 ± 0.589
1.284GlnMet: 1.284 ± 0.332
1.587GlnAsn: 1.587 ± 0.297
2.795GlnPro: 2.795 ± 0.364
3.022GlnGln: 3.022 ± 0.595
2.569GlnArg: 2.569 ± 0.376
2.418GlnSer: 2.418 ± 0.427
2.493GlnThr: 2.493 ± 0.411
2.267GlnVal: 2.267 ± 0.406
0.378GlnTrp: 0.378 ± 0.172
1.058GlnTyr: 1.058 ± 0.277
0.0GlnXaa: 0.0 ± 0.0
Arg
8.688ArgAla: 8.688 ± 0.926
0.907ArgCys: 0.907 ± 0.275
4.533ArgAsp: 4.533 ± 0.512
3.853ArgGlu: 3.853 ± 0.594
2.342ArgPhe: 2.342 ± 0.432
3.4ArgGly: 3.4 ± 0.483
1.209ArgHis: 1.209 ± 0.396
2.115ArgIle: 2.115 ± 0.358
2.569ArgLys: 2.569 ± 0.467
5.591ArgLeu: 5.591 ± 0.685
1.738ArgMet: 1.738 ± 0.385
1.738ArgAsn: 1.738 ± 0.326
3.324ArgPro: 3.324 ± 0.436
2.947ArgGln: 2.947 ± 0.47
3.324ArgArg: 3.324 ± 0.497
1.889ArgSer: 1.889 ± 0.358
3.324ArgThr: 3.324 ± 0.468
5.364ArgVal: 5.364 ± 0.713
1.435ArgTrp: 1.435 ± 0.212
2.569ArgTyr: 2.569 ± 0.548
0.0ArgXaa: 0.0 ± 0.0
Ser
6.951SerAla: 6.951 ± 0.694
0.529SerCys: 0.529 ± 0.195
2.871SerAsp: 2.871 ± 0.491
2.795SerGlu: 2.795 ± 0.521
2.418SerPhe: 2.418 ± 0.372
6.724SerGly: 6.724 ± 1.175
0.453SerHis: 0.453 ± 0.198
2.871SerIle: 2.871 ± 0.498
1.813SerLys: 1.813 ± 0.405
4.458SerLeu: 4.458 ± 0.474
1.284SerMet: 1.284 ± 0.297
2.267SerAsn: 2.267 ± 0.365
2.418SerPro: 2.418 ± 0.349
2.342SerGln: 2.342 ± 0.341
2.795SerArg: 2.795 ± 0.429
3.249SerSer: 3.249 ± 0.574
3.626SerThr: 3.626 ± 0.463
4.155SerVal: 4.155 ± 0.511
0.831SerTrp: 0.831 ± 0.258
1.662SerTyr: 1.662 ± 0.353
0.0SerXaa: 0.0 ± 0.0
Thr
6.875ThrAla: 6.875 ± 0.926
0.529ThrCys: 0.529 ± 0.175
3.249ThrAsp: 3.249 ± 0.557
2.342ThrGlu: 2.342 ± 0.41
2.115ThrPhe: 2.115 ± 0.341
6.497ThrGly: 6.497 ± 1.057
0.907ThrHis: 0.907 ± 0.303
3.929ThrIle: 3.929 ± 0.575
2.115ThrLys: 2.115 ± 0.388
5.062ThrLeu: 5.062 ± 0.507
1.284ThrMet: 1.284 ± 0.235
2.644ThrAsn: 2.644 ± 0.485
4.835ThrPro: 4.835 ± 0.605
2.72ThrGln: 2.72 ± 0.44
4.835ThrArg: 4.835 ± 0.622
3.702ThrSer: 3.702 ± 0.708
5.289ThrThr: 5.289 ± 1.061
5.213ThrVal: 5.213 ± 0.664
0.907ThrTrp: 0.907 ± 0.28
2.115ThrTyr: 2.115 ± 0.415
0.0ThrXaa: 0.0 ± 0.0
Val
8.235ValAla: 8.235 ± 0.746
0.907ValCys: 0.907 ± 0.237
4.155ValAsp: 4.155 ± 0.621
3.551ValGlu: 3.551 ± 0.573
2.644ValPhe: 2.644 ± 0.409
4.684ValGly: 4.684 ± 0.487
1.36ValHis: 1.36 ± 0.307
2.191ValIle: 2.191 ± 0.343
2.644ValLys: 2.644 ± 0.387
8.008ValLeu: 8.008 ± 0.542
1.511ValMet: 1.511 ± 0.375
2.342ValAsn: 2.342 ± 0.433
4.306ValPro: 4.306 ± 0.566
3.4ValGln: 3.4 ± 0.593
3.778ValArg: 3.778 ± 0.468
4.306ValSer: 4.306 ± 0.664
4.533ValThr: 4.533 ± 0.688
6.044ValVal: 6.044 ± 0.584
0.982ValTrp: 0.982 ± 0.267
1.889ValTyr: 1.889 ± 0.375
0.0ValXaa: 0.0 ± 0.0
Trp
1.738TrpAla: 1.738 ± 0.367
0.453TrpCys: 0.453 ± 0.177
0.453TrpAsp: 0.453 ± 0.14
0.604TrpGlu: 0.604 ± 0.23
0.604TrpPhe: 0.604 ± 0.203
1.058TrpGly: 1.058 ± 0.245
0.68TrpHis: 0.68 ± 0.276
0.227TrpIle: 0.227 ± 0.115
0.302TrpLys: 0.302 ± 0.167
1.511TrpLeu: 1.511 ± 0.331
0.453TrpMet: 0.453 ± 0.22
0.529TrpAsn: 0.529 ± 0.161
0.453TrpPro: 0.453 ± 0.181
0.68TrpGln: 0.68 ± 0.219
1.587TrpArg: 1.587 ± 0.364
0.907TrpSer: 0.907 ± 0.338
0.982TrpThr: 0.982 ± 0.365
1.058TrpVal: 1.058 ± 0.253
0.453TrpTrp: 0.453 ± 0.166
0.453TrpTyr: 0.453 ± 0.141
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.004TyrAla: 4.004 ± 0.57
0.604TyrCys: 0.604 ± 0.212
2.191TyrAsp: 2.191 ± 0.435
1.209TyrGlu: 1.209 ± 0.257
0.68TyrPhe: 0.68 ± 0.194
2.644TyrGly: 2.644 ± 0.444
0.68TyrHis: 0.68 ± 0.295
0.982TyrIle: 0.982 ± 0.258
0.756TyrLys: 0.756 ± 0.287
2.267TyrLeu: 2.267 ± 0.334
0.529TyrMet: 0.529 ± 0.25
1.587TyrAsn: 1.587 ± 0.276
1.435TyrPro: 1.435 ± 0.238
1.133TyrGln: 1.133 ± 0.272
2.04TyrArg: 2.04 ± 0.458
1.813TyrSer: 1.813 ± 0.342
1.662TyrThr: 1.662 ± 0.299
1.587TyrVal: 1.587 ± 0.321
0.302TyrTrp: 0.302 ± 0.124
0.529TyrTyr: 0.529 ± 0.211
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (13237 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski