Amino acid dipepetide frequency for Caulobacter phage CcrBL9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.256AlaAla: 12.256 ± 0.61
0.989AlaCys: 0.989 ± 0.108
5.993AlaAsp: 5.993 ± 0.263
6.859AlaGlu: 6.859 ± 0.352
4.07AlaPhe: 4.07 ± 0.235
8.838AlaGly: 8.838 ± 0.656
2.215AlaHis: 2.215 ± 0.182
5.6AlaIle: 5.6 ± 0.256
5.532AlaLys: 5.532 ± 0.321
10.131AlaLeu: 10.131 ± 0.384
3.25AlaMet: 3.25 ± 0.238
3.205AlaAsn: 3.205 ± 0.24
5.24AlaPro: 5.24 ± 0.275
4.127AlaGln: 4.127 ± 0.288
6.454AlaArg: 6.454 ± 0.306
5.442AlaSer: 5.442 ± 0.34
5.746AlaThr: 5.746 ± 0.331
7.399AlaVal: 7.399 ± 0.304
2.204AlaTrp: 2.204 ± 0.176
3.688AlaTyr: 3.688 ± 0.242
0.0AlaXaa: 0.0 ± 0.0
Cys
0.776CysAla: 0.776 ± 0.102
0.169CysCys: 0.169 ± 0.038
0.675CysAsp: 0.675 ± 0.084
0.697CysGlu: 0.697 ± 0.093
0.382CysPhe: 0.382 ± 0.064
0.832CysGly: 0.832 ± 0.106
0.247CysHis: 0.247 ± 0.05
0.292CysIle: 0.292 ± 0.066
0.439CysLys: 0.439 ± 0.086
0.697CysLeu: 0.697 ± 0.088
0.236CysMet: 0.236 ± 0.047
0.191CysAsn: 0.191 ± 0.051
0.405CysPro: 0.405 ± 0.067
0.439CysGln: 0.439 ± 0.081
0.596CysArg: 0.596 ± 0.092
0.405CysSer: 0.405 ± 0.063
0.461CysThr: 0.461 ± 0.069
0.641CysVal: 0.641 ± 0.098
0.18CysTrp: 0.18 ± 0.046
0.292CysTyr: 0.292 ± 0.058
0.0CysXaa: 0.0 ± 0.0
Asp
7.084AspAla: 7.084 ± 0.351
0.618AspCys: 0.618 ± 0.083
4.295AspAsp: 4.295 ± 0.316
4.655AspGlu: 4.655 ± 0.338
2.811AspPhe: 2.811 ± 0.175
5.903AspGly: 5.903 ± 0.3
1.54AspHis: 1.54 ± 0.133
3.081AspIle: 3.081 ± 0.198
2.586AspLys: 2.586 ± 0.211
6.477AspLeu: 6.477 ± 0.278
1.855AspMet: 1.855 ± 0.162
1.754AspAsn: 1.754 ± 0.139
3.879AspPro: 3.879 ± 0.22
2.204AspGln: 2.204 ± 0.158
4.16AspArg: 4.16 ± 0.274
2.631AspSer: 2.631 ± 0.162
3.103AspThr: 3.103 ± 0.215
4.16AspVal: 4.16 ± 0.26
1.372AspTrp: 1.372 ± 0.12
2.339AspTyr: 2.339 ± 0.18
0.0AspXaa: 0.0 ± 0.0
Glu
8.501GluAla: 8.501 ± 0.351
0.371GluCys: 0.371 ± 0.065
4.891GluAsp: 4.891 ± 0.317
3.89GluGlu: 3.89 ± 0.276
2.35GluPhe: 2.35 ± 0.182
4.329GluGly: 4.329 ± 0.228
2.103GluHis: 2.103 ± 0.184
3.351GluIle: 3.351 ± 0.204
2.35GluLys: 2.35 ± 0.189
3.722GluLeu: 3.722 ± 0.229
1.473GluMet: 1.473 ± 0.126
1.675GluAsn: 1.675 ± 0.148
3.295GluPro: 3.295 ± 0.207
2.204GluGln: 2.204 ± 0.193
4.644GluArg: 4.644 ± 0.297
2.305GluSer: 2.305 ± 0.162
3.722GluThr: 3.722 ± 0.214
4.756GluVal: 4.756 ± 0.267
1.124GluTrp: 1.124 ± 0.122
1.585GluTyr: 1.585 ± 0.15
0.0GluXaa: 0.0 ± 0.0
Phe
3.328PheAla: 3.328 ± 0.187
0.483PheCys: 0.483 ± 0.077
3.238PheAsp: 3.238 ± 0.223
2.564PheGlu: 2.564 ± 0.165
1.091PhePhe: 1.091 ± 0.12
3.16PheGly: 3.16 ± 0.206
0.821PheHis: 0.821 ± 0.089
1.383PheIle: 1.383 ± 0.139
1.72PheLys: 1.72 ± 0.153
2.98PheLeu: 2.98 ± 0.189
1.169PheMet: 1.169 ± 0.103
1.349PheAsn: 1.349 ± 0.112
1.54PhePro: 1.54 ± 0.129
1.406PheGln: 1.406 ± 0.121
1.912PheArg: 1.912 ± 0.153
2.024PheSer: 2.024 ± 0.183
2.193PheThr: 2.193 ± 0.196
2.53PheVal: 2.53 ± 0.157
0.618PheTrp: 0.618 ± 0.092
1.169PheTyr: 1.169 ± 0.116
0.0PheXaa: 0.0 ± 0.0
Gly
8.175GlyAla: 8.175 ± 0.63
0.776GlyCys: 0.776 ± 0.087
5.06GlyAsp: 5.06 ± 0.205
4.756GlyGlu: 4.756 ± 0.233
3.182GlyPhe: 3.182 ± 0.246
8.928GlyGly: 8.928 ± 2.152
1.698GlyHis: 1.698 ± 0.14
3.047GlyIle: 3.047 ± 0.188
3.688GlyLys: 3.688 ± 0.23
6.229GlyLeu: 6.229 ± 0.34
2.001GlyMet: 2.001 ± 0.176
2.406GlyAsn: 2.406 ± 0.312
2.811GlyPro: 2.811 ± 0.17
3.148GlyGln: 3.148 ± 0.28
4.441GlyArg: 4.441 ± 0.217
5.026GlySer: 5.026 ± 0.513
4.711GlyThr: 4.711 ± 0.579
5.926GlyVal: 5.926 ± 0.314
1.844GlyTrp: 1.844 ± 0.14
3.396GlyTyr: 3.396 ± 0.23
0.0GlyXaa: 0.0 ± 0.0
His
2.373HisAla: 2.373 ± 0.175
0.27HisCys: 0.27 ± 0.057
1.338HisAsp: 1.338 ± 0.146
1.585HisGlu: 1.585 ± 0.127
0.933HisPhe: 0.933 ± 0.114
1.743HisGly: 1.743 ± 0.134
0.708HisHis: 0.708 ± 0.111
0.933HisIle: 0.933 ± 0.109
0.956HisLys: 0.956 ± 0.117
1.956HisLeu: 1.956 ± 0.161
0.618HisMet: 0.618 ± 0.087
0.618HisAsn: 0.618 ± 0.078
1.327HisPro: 1.327 ± 0.131
0.697HisGln: 0.697 ± 0.098
1.563HisArg: 1.563 ± 0.145
0.877HisSer: 0.877 ± 0.095
1.181HisThr: 1.181 ± 0.128
1.54HisVal: 1.54 ± 0.143
0.427HisTrp: 0.427 ± 0.07
0.911HisTyr: 0.911 ± 0.096
0.0HisXaa: 0.0 ± 0.0
Ile
5.701IleAla: 5.701 ± 0.246
0.472IleCys: 0.472 ± 0.072
3.935IleAsp: 3.935 ± 0.238
4.037IleGlu: 4.037 ± 0.23
1.428IlePhe: 1.428 ± 0.131
3.756IleGly: 3.756 ± 0.261
0.967IleHis: 0.967 ± 0.124
2.215IleIle: 2.215 ± 0.157
2.631IleLys: 2.631 ± 0.206
3.801IleLeu: 3.801 ± 0.195
0.989IleMet: 0.989 ± 0.106
1.912IleAsn: 1.912 ± 0.158
2.35IlePro: 2.35 ± 0.166
1.574IleGln: 1.574 ± 0.136
2.777IleArg: 2.777 ± 0.184
2.328IleSer: 2.328 ± 0.183
3.373IleThr: 3.373 ± 0.334
3.699IleVal: 3.699 ± 0.206
0.641IleTrp: 0.641 ± 0.088
1.417IleTyr: 1.417 ± 0.121
0.0IleXaa: 0.0 ± 0.0
Lys
5.993LysAla: 5.993 ± 0.343
0.124LysCys: 0.124 ± 0.041
3.013LysAsp: 3.013 ± 0.251
2.485LysGlu: 2.485 ± 0.185
1.259LysPhe: 1.259 ± 0.124
3.126LysGly: 3.126 ± 0.161
1.248LysHis: 1.248 ± 0.135
2.406LysIle: 2.406 ± 0.175
2.485LysLys: 2.485 ± 0.203
4.138LysLeu: 4.138 ± 0.267
0.989LysMet: 0.989 ± 0.11
1.259LysAsn: 1.259 ± 0.12
2.766LysPro: 2.766 ± 0.213
1.417LysGln: 1.417 ± 0.13
2.867LysArg: 2.867 ± 0.227
1.844LysSer: 1.844 ± 0.163
2.912LysThr: 2.912 ± 0.214
3.418LysVal: 3.418 ± 0.214
0.697LysTrp: 0.697 ± 0.085
0.866LysTyr: 0.866 ± 0.11
0.0LysXaa: 0.0 ± 0.0
Leu
9.58LeuAla: 9.58 ± 0.365
0.888LeuCys: 0.888 ± 0.104
5.645LeuAsp: 5.645 ± 0.276
4.678LeuGlu: 4.678 ± 0.266
2.552LeuPhe: 2.552 ± 0.203
6.117LeuGly: 6.117 ± 0.348
1.945LeuHis: 1.945 ± 0.151
4.351LeuIle: 4.351 ± 0.242
3.913LeuLys: 3.913 ± 0.244
6.544LeuLeu: 6.544 ± 0.325
2.08LeuMet: 2.08 ± 0.135
2.867LeuAsn: 2.867 ± 0.204
3.98LeuPro: 3.98 ± 0.198
2.44LeuGln: 2.44 ± 0.183
5.847LeuArg: 5.847 ± 0.334
4.947LeuSer: 4.947 ± 0.306
6.049LeuThr: 6.049 ± 0.248
5.408LeuVal: 5.408 ± 0.263
1.113LeuTrp: 1.113 ± 0.105
2.429LeuTyr: 2.429 ± 0.189
0.0LeuXaa: 0.0 ± 0.0
Met
2.777MetAla: 2.777 ± 0.177
0.101MetCys: 0.101 ± 0.031
1.181MetAsp: 1.181 ± 0.102
1.158MetGlu: 1.158 ± 0.129
0.652MetPhe: 0.652 ± 0.087
1.833MetGly: 1.833 ± 0.186
0.371MetHis: 0.371 ± 0.064
1.653MetIle: 1.653 ± 0.149
1.226MetLys: 1.226 ± 0.135
2.215MetLeu: 2.215 ± 0.165
0.54MetMet: 0.54 ± 0.088
0.753MetAsn: 0.753 ± 0.097
1.45MetPro: 1.45 ± 0.114
0.866MetGln: 0.866 ± 0.1
1.563MetArg: 1.563 ± 0.143
1.912MetSer: 1.912 ± 0.145
2.384MetThr: 2.384 ± 0.181
1.361MetVal: 1.361 ± 0.128
0.349MetTrp: 0.349 ± 0.063
0.214MetTyr: 0.214 ± 0.056
0.0MetXaa: 0.0 ± 0.0
Asn
3.688AsnAla: 3.688 ± 0.28
0.191AsnCys: 0.191 ± 0.045
1.833AsnAsp: 1.833 ± 0.124
1.529AsnGlu: 1.529 ± 0.136
0.989AsnPhe: 0.989 ± 0.102
3.373AsnGly: 3.373 ± 0.422
0.787AsnHis: 0.787 ± 0.103
1.507AsnIle: 1.507 ± 0.17
1.023AsnLys: 1.023 ± 0.127
2.642AsnLeu: 2.642 ± 0.209
0.562AsnMet: 0.562 ± 0.076
0.843AsnAsn: 0.843 ± 0.119
2.114AsnPro: 2.114 ± 0.143
0.967AsnGln: 0.967 ± 0.096
1.945AsnArg: 1.945 ± 0.158
1.214AsnSer: 1.214 ± 0.136
1.833AsnThr: 1.833 ± 0.177
2.024AsnVal: 2.024 ± 0.147
0.686AsnTrp: 0.686 ± 0.089
0.956AsnTyr: 0.956 ± 0.105
0.0AsnXaa: 0.0 ± 0.0
Pro
5.543ProAla: 5.543 ± 0.236
0.45ProCys: 0.45 ± 0.076
3.632ProAsp: 3.632 ± 0.21
3.576ProGlu: 3.576 ± 0.19
1.912ProPhe: 1.912 ± 0.156
4.014ProGly: 4.014 ± 0.219
1.046ProHis: 1.046 ± 0.12
2.519ProIle: 2.519 ± 0.173
2.26ProLys: 2.26 ± 0.177
3.789ProLeu: 3.789 ± 0.223
1.068ProMet: 1.068 ± 0.118
1.81ProAsn: 1.81 ± 0.142
2.766ProPro: 2.766 ± 0.299
1.72ProGln: 1.72 ± 0.133
2.867ProArg: 2.867 ± 0.228
2.631ProSer: 2.631 ± 0.16
3.036ProThr: 3.036 ± 0.243
3.587ProVal: 3.587 ± 0.199
0.81ProTrp: 0.81 ± 0.091
1.653ProTyr: 1.653 ± 0.145
0.0ProXaa: 0.0 ± 0.0
Gln
4.351GlnAla: 4.351 ± 0.264
0.202GlnCys: 0.202 ± 0.046
2.193GlnAsp: 2.193 ± 0.161
2.013GlnGlu: 2.013 ± 0.154
1.394GlnPhe: 1.394 ± 0.135
2.98GlnGly: 2.98 ± 0.184
0.686GlnHis: 0.686 ± 0.1
1.945GlnIle: 1.945 ± 0.171
1.63GlnLys: 1.63 ± 0.166
2.001GlnLeu: 2.001 ± 0.185
0.911GlnMet: 0.911 ± 0.114
0.9GlnAsn: 0.9 ± 0.118
1.72GlnPro: 1.72 ± 0.139
1.293GlnGln: 1.293 ± 0.147
2.215GlnArg: 2.215 ± 0.156
1.597GlnSer: 1.597 ± 0.152
2.08GlnThr: 2.08 ± 0.149
2.699GlnVal: 2.699 ± 0.147
0.551GlnTrp: 0.551 ± 0.077
0.832GlnTyr: 0.832 ± 0.092
0.0GlnXaa: 0.0 ± 0.0
Arg
6.229ArgAla: 6.229 ± 0.311
0.663ArgCys: 0.663 ± 0.102
4.43ArgAsp: 4.43 ± 0.298
3.879ArgGlu: 3.879 ± 0.273
2.609ArgPhe: 2.609 ± 0.184
3.722ArgGly: 3.722 ± 0.205
1.406ArgHis: 1.406 ± 0.126
3.34ArgIle: 3.34 ± 0.178
2.923ArgLys: 2.923 ± 0.23
6.218ArgLeu: 6.218 ± 0.344
1.72ArgMet: 1.72 ± 0.155
1.765ArgAsn: 1.765 ± 0.145
2.991ArgPro: 2.991 ± 0.214
2.384ArgGln: 2.384 ± 0.17
4.947ArgArg: 4.947 ± 0.358
2.53ArgSer: 2.53 ± 0.186
3.16ArgThr: 3.16 ± 0.202
4.25ArgVal: 4.25 ± 0.213
1.304ArgTrp: 1.304 ± 0.137
2.496ArgTyr: 2.496 ± 0.176
0.0ArgXaa: 0.0 ± 0.0
Ser
4.914SerAla: 4.914 ± 0.295
0.461SerCys: 0.461 ± 0.062
3.16SerAsp: 3.16 ± 0.191
2.541SerGlu: 2.541 ± 0.176
2.328SerPhe: 2.328 ± 0.186
4.7SerGly: 4.7 ± 0.526
1.102SerHis: 1.102 ± 0.113
2.564SerIle: 2.564 ± 0.151
2.249SerLys: 2.249 ± 0.202
4.329SerLeu: 4.329 ± 0.212
1.158SerMet: 1.158 ± 0.124
1.585SerAsn: 1.585 ± 0.176
2.744SerPro: 2.744 ± 0.173
1.529SerGln: 1.529 ± 0.148
2.766SerArg: 2.766 ± 0.176
2.968SerSer: 2.968 ± 0.305
3.216SerThr: 3.216 ± 0.228
3.238SerVal: 3.238 ± 0.212
0.956SerTrp: 0.956 ± 0.116
2.091SerTyr: 2.091 ± 0.144
0.0SerXaa: 0.0 ± 0.0
Thr
5.881ThrAla: 5.881 ± 0.326
0.551ThrCys: 0.551 ± 0.088
3.812ThrAsp: 3.812 ± 0.271
3.34ThrGlu: 3.34 ± 0.205
2.384ThrPhe: 2.384 ± 0.198
5.116ThrGly: 5.116 ± 0.483
1.113ThrHis: 1.113 ± 0.121
4.014ThrIle: 4.014 ± 0.384
2.373ThrLys: 2.373 ± 0.158
5.341ThrLeu: 5.341 ± 0.297
1.327ThrMet: 1.327 ± 0.119
1.833ThrAsn: 1.833 ± 0.181
3.958ThrPro: 3.958 ± 0.296
1.698ThrGln: 1.698 ± 0.142
3.576ThrArg: 3.576 ± 0.2
3.283ThrSer: 3.283 ± 0.229
3.598ThrThr: 3.598 ± 0.31
4.228ThrVal: 4.228 ± 0.298
0.945ThrTrp: 0.945 ± 0.099
2.328ThrTyr: 2.328 ± 0.16
0.0ThrXaa: 0.0 ± 0.0
Val
6.904ValAla: 6.904 ± 0.255
0.675ValCys: 0.675 ± 0.097
4.408ValAsp: 4.408 ± 0.243
5.161ValGlu: 5.161 ± 0.27
2.552ValPhe: 2.552 ± 0.164
4.655ValGly: 4.655 ± 0.265
1.383ValHis: 1.383 ± 0.146
3.497ValIle: 3.497 ± 0.244
3.216ValLys: 3.216 ± 0.175
6.353ValLeu: 6.353 ± 0.358
1.45ValMet: 1.45 ± 0.145
2.26ValAsn: 2.26 ± 0.208
3.306ValPro: 3.306 ± 0.175
2.361ValGln: 2.361 ± 0.167
4.396ValArg: 4.396 ± 0.251
4.003ValSer: 4.003 ± 0.313
4.678ValThr: 4.678 ± 0.249
4.531ValVal: 4.531 ± 0.271
1.181ValTrp: 1.181 ± 0.1
2.058ValTyr: 2.058 ± 0.161
0.0ValXaa: 0.0 ± 0.0
Trp
1.687TrpAla: 1.687 ± 0.146
0.202TrpCys: 0.202 ± 0.049
1.034TrpAsp: 1.034 ± 0.097
1.023TrpGlu: 1.023 ± 0.122
0.731TrpPhe: 0.731 ± 0.09
1.259TrpGly: 1.259 ± 0.137
0.36TrpHis: 0.36 ± 0.061
0.945TrpIle: 0.945 ± 0.121
0.989TrpLys: 0.989 ± 0.114
1.372TrpLeu: 1.372 ± 0.111
0.551TrpMet: 0.551 ± 0.076
0.675TrpAsn: 0.675 ± 0.09
0.607TrpPro: 0.607 ± 0.085
0.618TrpGln: 0.618 ± 0.096
1.383TrpArg: 1.383 ± 0.135
1.091TrpSer: 1.091 ± 0.118
1.383TrpThr: 1.383 ± 0.132
1.214TrpVal: 1.214 ± 0.113
0.36TrpTrp: 0.36 ± 0.072
0.394TrpTyr: 0.394 ± 0.073
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.497TyrAla: 3.497 ± 0.24
0.394TyrCys: 0.394 ± 0.063
2.744TyrAsp: 2.744 ± 0.19
2.215TyrGlu: 2.215 ± 0.198
1.068TyrPhe: 1.068 ± 0.111
2.867TyrGly: 2.867 ± 0.174
0.81TyrHis: 0.81 ± 0.106
1.361TyrIle: 1.361 ± 0.129
1.124TyrLys: 1.124 ± 0.109
2.44TyrLeu: 2.44 ± 0.168
0.596TyrMet: 0.596 ± 0.077
1.046TyrAsn: 1.046 ± 0.112
1.439TyrPro: 1.439 ± 0.137
1.113TyrGln: 1.113 ± 0.132
2.046TyrArg: 2.046 ± 0.135
1.54TyrSer: 1.54 ± 0.12
1.878TyrThr: 1.878 ± 0.163
2.417TyrVal: 2.417 ± 0.175
0.517TyrTrp: 0.517 ± 0.083
0.956TyrTyr: 0.956 ± 0.121
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 517 proteins (88936 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski