Amino acid dipepetide frequency for Arthrobacter phage Kumotta

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.631AlaAla: 16.631 ± 1.501
0.634AlaCys: 0.634 ± 0.246
6.336AlaAsp: 6.336 ± 0.835
7.524AlaGlu: 7.524 ± 0.95
3.485AlaPhe: 3.485 ± 0.907
9.979AlaGly: 9.979 ± 1.18
1.742AlaHis: 1.742 ± 0.37
4.277AlaIle: 4.277 ± 0.484
5.069AlaLys: 5.069 ± 0.772
9.899AlaLeu: 9.899 ± 1.077
3.326AlaMet: 3.326 ± 0.454
3.801AlaAsn: 3.801 ± 0.476
5.148AlaPro: 5.148 ± 0.601
4.831AlaGln: 4.831 ± 0.7
7.682AlaArg: 7.682 ± 1.102
6.098AlaSer: 6.098 ± 0.583
6.336AlaThr: 6.336 ± 0.544
7.999AlaVal: 7.999 ± 0.842
1.821AlaTrp: 1.821 ± 0.343
1.742AlaTyr: 1.742 ± 0.393
0.0AlaXaa: 0.0 ± 0.0
Cys
0.634CysAla: 0.634 ± 0.217
0.158CysCys: 0.158 ± 0.13
0.634CysAsp: 0.634 ± 0.248
0.634CysGlu: 0.634 ± 0.24
0.317CysPhe: 0.317 ± 0.172
1.505CysGly: 1.505 ± 0.502
0.238CysHis: 0.238 ± 0.143
0.475CysIle: 0.475 ± 0.211
0.713CysLys: 0.713 ± 0.215
0.475CysLeu: 0.475 ± 0.2
0.0CysMet: 0.0 ± 0.0
0.713CysAsn: 0.713 ± 0.242
0.713CysPro: 0.713 ± 0.292
0.396CysGln: 0.396 ± 0.162
0.871CysArg: 0.871 ± 0.333
0.475CysSer: 0.475 ± 0.195
0.554CysThr: 0.554 ± 0.243
0.634CysVal: 0.634 ± 0.242
0.317CysTrp: 0.317 ± 0.148
0.079CysTyr: 0.079 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
6.336AspAla: 6.336 ± 0.832
0.713AspCys: 0.713 ± 0.258
4.356AspAsp: 4.356 ± 0.71
4.277AspGlu: 4.277 ± 0.624
1.821AspPhe: 1.821 ± 0.303
7.048AspGly: 7.048 ± 0.728
1.98AspHis: 1.98 ± 0.383
2.772AspIle: 2.772 ± 0.397
2.772AspLys: 2.772 ± 0.514
5.464AspLeu: 5.464 ± 0.678
1.109AspMet: 1.109 ± 0.278
1.426AspAsn: 1.426 ± 0.284
3.247AspPro: 3.247 ± 0.482
2.455AspGln: 2.455 ± 0.367
2.693AspArg: 2.693 ± 0.516
2.455AspSer: 2.455 ± 0.58
3.089AspThr: 3.089 ± 0.492
3.643AspVal: 3.643 ± 0.471
1.584AspTrp: 1.584 ± 0.411
1.901AspTyr: 1.901 ± 0.353
0.0AspXaa: 0.0 ± 0.0
Glu
8.632GluAla: 8.632 ± 1.032
1.188GluCys: 1.188 ± 0.331
3.801GluAsp: 3.801 ± 0.588
3.009GluGlu: 3.009 ± 0.661
1.98GluPhe: 1.98 ± 0.325
4.197GluGly: 4.197 ± 0.51
1.346GluHis: 1.346 ± 0.323
3.168GluIle: 3.168 ± 0.56
1.98GluLys: 1.98 ± 0.451
5.227GluLeu: 5.227 ± 0.639
1.584GluMet: 1.584 ± 0.364
2.217GluAsn: 2.217 ± 0.442
3.881GluPro: 3.881 ± 0.563
2.772GluGln: 2.772 ± 0.439
4.831GluArg: 4.831 ± 0.781
3.009GluSer: 3.009 ± 0.515
4.039GluThr: 4.039 ± 0.626
3.247GluVal: 3.247 ± 0.448
1.821GluTrp: 1.821 ± 0.419
1.742GluTyr: 1.742 ± 0.319
0.0GluXaa: 0.0 ± 0.0
Phe
2.693PheAla: 2.693 ± 0.627
0.238PheCys: 0.238 ± 0.144
2.376PheAsp: 2.376 ± 0.453
1.663PheGlu: 1.663 ± 0.391
1.426PhePhe: 1.426 ± 0.338
3.643PheGly: 3.643 ± 0.631
0.95PheHis: 0.95 ± 0.236
1.584PheIle: 1.584 ± 0.494
1.346PheLys: 1.346 ± 0.269
1.426PheLeu: 1.426 ± 0.274
0.792PheMet: 0.792 ± 0.199
0.554PheAsn: 0.554 ± 0.165
1.188PhePro: 1.188 ± 0.301
1.346PheGln: 1.346 ± 0.323
1.901PheArg: 1.901 ± 0.485
1.901PheSer: 1.901 ± 0.373
2.376PheThr: 2.376 ± 0.46
2.059PheVal: 2.059 ± 0.43
0.95PheTrp: 0.95 ± 0.264
0.396PheTyr: 0.396 ± 0.17
0.0PheXaa: 0.0 ± 0.0
Gly
7.999GlyAla: 7.999 ± 1.13
0.554GlyCys: 0.554 ± 0.185
5.069GlyAsp: 5.069 ± 0.495
5.148GlyGlu: 5.148 ± 0.508
2.534GlyPhe: 2.534 ± 0.452
8.711GlyGly: 8.711 ± 1.339
2.297GlyHis: 2.297 ± 0.438
4.673GlyIle: 4.673 ± 0.676
4.277GlyLys: 4.277 ± 0.514
7.444GlyLeu: 7.444 ± 1.088
2.534GlyMet: 2.534 ± 0.413
2.93GlyAsn: 2.93 ± 0.443
4.118GlyPro: 4.118 ± 0.415
2.93GlyGln: 2.93 ± 0.527
4.356GlyArg: 4.356 ± 0.619
5.781GlySer: 5.781 ± 0.721
4.356GlyThr: 4.356 ± 0.71
5.86GlyVal: 5.86 ± 0.752
2.93GlyTrp: 2.93 ± 0.485
3.009GlyTyr: 3.009 ± 0.593
0.0GlyXaa: 0.0 ± 0.0
His
1.584HisAla: 1.584 ± 0.323
0.475HisCys: 0.475 ± 0.202
1.188HisAsp: 1.188 ± 0.357
1.584HisGlu: 1.584 ± 0.388
0.554HisPhe: 0.554 ± 0.19
1.742HisGly: 1.742 ± 0.383
0.95HisHis: 0.95 ± 0.293
1.03HisIle: 1.03 ± 0.333
0.475HisLys: 0.475 ± 0.187
2.455HisLeu: 2.455 ± 0.452
0.317HisMet: 0.317 ± 0.138
0.713HisAsn: 0.713 ± 0.2
1.742HisPro: 1.742 ± 0.333
1.188HisGln: 1.188 ± 0.305
1.346HisArg: 1.346 ± 0.384
0.792HisSer: 0.792 ± 0.292
1.267HisThr: 1.267 ± 0.359
1.267HisVal: 1.267 ± 0.284
0.475HisTrp: 0.475 ± 0.211
0.396HisTyr: 0.396 ± 0.165
0.0HisXaa: 0.0 ± 0.0
Ile
4.197IleAla: 4.197 ± 0.593
0.396IleCys: 0.396 ± 0.181
2.851IleAsp: 2.851 ± 0.421
3.485IleGlu: 3.485 ± 0.602
1.426IlePhe: 1.426 ± 0.355
4.831IleGly: 4.831 ± 0.85
1.109IleHis: 1.109 ± 0.324
1.901IleIle: 1.901 ± 0.383
2.217IleLys: 2.217 ± 0.341
2.772IleLeu: 2.772 ± 0.502
0.713IleMet: 0.713 ± 0.243
1.346IleAsn: 1.346 ± 0.299
2.613IlePro: 2.613 ± 0.423
1.901IleGln: 1.901 ± 0.332
2.613IleArg: 2.613 ± 0.413
2.693IleSer: 2.693 ± 0.394
4.197IleThr: 4.197 ± 0.536
3.247IleVal: 3.247 ± 0.479
0.396IleTrp: 0.396 ± 0.21
1.109IleTyr: 1.109 ± 0.297
0.0IleXaa: 0.0 ± 0.0
Lys
6.732LysAla: 6.732 ± 0.802
0.634LysCys: 0.634 ± 0.216
2.93LysAsp: 2.93 ± 0.586
2.297LysGlu: 2.297 ± 0.412
0.95LysPhe: 0.95 ± 0.265
3.009LysGly: 3.009 ± 0.467
0.871LysHis: 0.871 ± 0.268
1.426LysIle: 1.426 ± 0.348
2.217LysLys: 2.217 ± 0.465
3.247LysLeu: 3.247 ± 0.504
1.109LysMet: 1.109 ± 0.265
1.188LysAsn: 1.188 ± 0.315
2.613LysPro: 2.613 ± 0.497
1.426LysGln: 1.426 ± 0.379
3.405LysArg: 3.405 ± 0.567
2.455LysSer: 2.455 ± 0.518
3.564LysThr: 3.564 ± 0.455
3.405LysVal: 3.405 ± 0.479
1.109LysTrp: 1.109 ± 0.297
0.95LysTyr: 0.95 ± 0.265
0.0LysXaa: 0.0 ± 0.0
Leu
10.929LeuAla: 10.929 ± 1.021
0.713LeuCys: 0.713 ± 0.239
4.277LeuAsp: 4.277 ± 0.599
5.227LeuGlu: 5.227 ± 0.586
1.663LeuPhe: 1.663 ± 0.315
5.623LeuGly: 5.623 ± 0.771
1.901LeuHis: 1.901 ± 0.355
3.247LeuIle: 3.247 ± 0.421
3.564LeuLys: 3.564 ± 0.459
5.623LeuLeu: 5.623 ± 0.712
1.663LeuMet: 1.663 ± 0.302
2.613LeuAsn: 2.613 ± 0.68
4.673LeuPro: 4.673 ± 0.615
1.98LeuGln: 1.98 ± 0.432
4.673LeuArg: 4.673 ± 0.647
3.643LeuSer: 3.643 ± 0.544
6.177LeuThr: 6.177 ± 0.71
5.227LeuVal: 5.227 ± 0.553
1.346LeuTrp: 1.346 ± 0.284
1.584LeuTyr: 1.584 ± 0.326
0.0LeuXaa: 0.0 ± 0.0
Met
2.851MetAla: 2.851 ± 0.445
0.238MetCys: 0.238 ± 0.152
1.188MetAsp: 1.188 ± 0.306
0.871MetGlu: 0.871 ± 0.283
0.554MetPhe: 0.554 ± 0.169
1.584MetGly: 1.584 ± 0.312
0.317MetHis: 0.317 ± 0.131
1.03MetIle: 1.03 ± 0.259
0.792MetLys: 0.792 ± 0.252
1.505MetLeu: 1.505 ± 0.396
0.475MetMet: 0.475 ± 0.187
0.554MetAsn: 0.554 ± 0.18
1.426MetPro: 1.426 ± 0.367
0.634MetGln: 0.634 ± 0.192
1.505MetArg: 1.505 ± 0.422
2.851MetSer: 2.851 ± 0.484
2.297MetThr: 2.297 ± 0.403
1.109MetVal: 1.109 ± 0.298
0.158MetTrp: 0.158 ± 0.089
0.554MetTyr: 0.554 ± 0.184
0.0MetXaa: 0.0 ± 0.0
Asn
3.009AsnAla: 3.009 ± 0.531
0.396AsnCys: 0.396 ± 0.182
1.663AsnAsp: 1.663 ± 0.364
1.663AsnGlu: 1.663 ± 0.384
0.792AsnPhe: 0.792 ± 0.184
3.722AsnGly: 3.722 ± 0.519
0.95AsnHis: 0.95 ± 0.334
1.267AsnIle: 1.267 ± 0.359
1.03AsnLys: 1.03 ± 0.331
2.693AsnLeu: 2.693 ± 0.461
0.634AsnMet: 0.634 ± 0.219
1.663AsnAsn: 1.663 ± 0.501
1.98AsnPro: 1.98 ± 0.367
1.584AsnGln: 1.584 ± 0.32
1.821AsnArg: 1.821 ± 0.33
1.426AsnSer: 1.426 ± 0.303
1.742AsnThr: 1.742 ± 0.45
2.217AsnVal: 2.217 ± 0.427
0.475AsnTrp: 0.475 ± 0.145
0.554AsnTyr: 0.554 ± 0.179
0.0AsnXaa: 0.0 ± 0.0
Pro
6.494ProAla: 6.494 ± 0.746
0.792ProCys: 0.792 ± 0.285
3.564ProAsp: 3.564 ± 0.65
4.514ProGlu: 4.514 ± 0.573
2.217ProPhe: 2.217 ± 0.398
5.464ProGly: 5.464 ± 0.791
0.792ProHis: 0.792 ± 0.211
2.059ProIle: 2.059 ± 0.397
2.851ProLys: 2.851 ± 0.568
3.881ProLeu: 3.881 ± 0.647
1.03ProMet: 1.03 ± 0.273
1.742ProAsn: 1.742 ± 0.364
2.613ProPro: 2.613 ± 0.396
1.505ProGln: 1.505 ± 0.296
1.98ProArg: 1.98 ± 0.334
3.247ProSer: 3.247 ± 0.456
3.881ProThr: 3.881 ± 0.511
3.722ProVal: 3.722 ± 0.494
1.03ProTrp: 1.03 ± 0.294
1.663ProTyr: 1.663 ± 0.341
0.0ProXaa: 0.0 ± 0.0
Gln
3.801GlnAla: 3.801 ± 0.496
0.475GlnCys: 0.475 ± 0.213
0.792GlnAsp: 0.792 ± 0.208
2.138GlnGlu: 2.138 ± 0.399
1.426GlnPhe: 1.426 ± 0.276
2.613GlnGly: 2.613 ± 0.439
0.634GlnHis: 0.634 ± 0.259
1.901GlnIle: 1.901 ± 0.369
1.505GlnLys: 1.505 ± 0.354
3.564GlnLeu: 3.564 ± 0.458
1.03GlnMet: 1.03 ± 0.252
0.554GlnAsn: 0.554 ± 0.268
2.93GlnPro: 2.93 ± 0.409
1.663GlnGln: 1.663 ± 0.453
2.851GlnArg: 2.851 ± 0.528
1.505GlnSer: 1.505 ± 0.279
2.455GlnThr: 2.455 ± 0.554
2.613GlnVal: 2.613 ± 0.513
1.03GlnTrp: 1.03 ± 0.243
1.03GlnTyr: 1.03 ± 0.281
0.0GlnXaa: 0.0 ± 0.0
Arg
6.415ArgAla: 6.415 ± 0.875
0.95ArgCys: 0.95 ± 0.281
5.148ArgAsp: 5.148 ± 0.611
4.197ArgGlu: 4.197 ± 0.656
1.821ArgPhe: 1.821 ± 0.331
3.247ArgGly: 3.247 ± 0.417
1.584ArgHis: 1.584 ± 0.425
3.881ArgIle: 3.881 ± 0.601
3.96ArgLys: 3.96 ± 0.558
3.96ArgLeu: 3.96 ± 0.637
1.267ArgMet: 1.267 ± 0.264
1.901ArgAsn: 1.901 ± 0.395
3.801ArgPro: 3.801 ± 0.58
2.217ArgGln: 2.217 ± 0.418
5.148ArgArg: 5.148 ± 0.975
2.693ArgSer: 2.693 ± 0.497
4.593ArgThr: 4.593 ± 0.6
4.356ArgVal: 4.356 ± 0.558
1.188ArgTrp: 1.188 ± 0.31
1.346ArgTyr: 1.346 ± 0.439
0.0ArgXaa: 0.0 ± 0.0
Ser
6.336SerAla: 6.336 ± 0.731
0.475SerCys: 0.475 ± 0.206
3.405SerAsp: 3.405 ± 0.493
3.405SerGlu: 3.405 ± 0.33
2.138SerPhe: 2.138 ± 0.554
5.306SerGly: 5.306 ± 0.632
1.03SerHis: 1.03 ± 0.288
2.376SerIle: 2.376 ± 0.405
2.059SerLys: 2.059 ± 0.407
4.277SerLeu: 4.277 ± 0.649
1.346SerMet: 1.346 ± 0.311
1.505SerAsn: 1.505 ± 0.382
2.693SerPro: 2.693 ± 0.463
1.742SerGln: 1.742 ± 0.362
3.881SerArg: 3.881 ± 0.538
3.009SerSer: 3.009 ± 0.72
3.326SerThr: 3.326 ± 0.483
3.009SerVal: 3.009 ± 0.484
1.03SerTrp: 1.03 ± 0.354
1.663SerTyr: 1.663 ± 0.358
0.0SerXaa: 0.0 ± 0.0
Thr
6.652ThrAla: 6.652 ± 0.661
0.634ThrCys: 0.634 ± 0.276
4.197ThrAsp: 4.197 ± 0.73
4.356ThrGlu: 4.356 ± 0.597
2.217ThrPhe: 2.217 ± 0.402
5.781ThrGly: 5.781 ± 0.888
1.109ThrHis: 1.109 ± 0.329
3.801ThrIle: 3.801 ± 0.487
3.326ThrLys: 3.326 ± 0.537
4.673ThrLeu: 4.673 ± 0.657
1.426ThrMet: 1.426 ± 0.325
2.376ThrAsn: 2.376 ± 0.588
3.881ThrPro: 3.881 ± 0.514
1.98ThrGln: 1.98 ± 0.361
3.326ThrArg: 3.326 ± 0.514
3.405ThrSer: 3.405 ± 0.563
5.227ThrThr: 5.227 ± 0.743
5.544ThrVal: 5.544 ± 0.787
1.426ThrTrp: 1.426 ± 0.268
1.98ThrTyr: 1.98 ± 0.34
0.0ThrXaa: 0.0 ± 0.0
Val
7.128ValAla: 7.128 ± 0.803
0.238ValCys: 0.238 ± 0.142
4.039ValAsp: 4.039 ± 0.557
4.593ValGlu: 4.593 ± 0.531
2.455ValPhe: 2.455 ± 0.568
5.148ValGly: 5.148 ± 0.715
0.871ValHis: 0.871 ± 0.23
3.643ValIle: 3.643 ± 0.553
3.564ValLys: 3.564 ± 0.478
4.277ValLeu: 4.277 ± 0.594
1.505ValMet: 1.505 ± 0.267
2.455ValAsn: 2.455 ± 0.403
3.168ValPro: 3.168 ± 0.63
1.584ValGln: 1.584 ± 0.415
5.306ValArg: 5.306 ± 0.602
3.722ValSer: 3.722 ± 0.453
4.989ValThr: 4.989 ± 0.654
4.831ValVal: 4.831 ± 0.672
1.584ValTrp: 1.584 ± 0.436
1.584ValTyr: 1.584 ± 0.376
0.0ValXaa: 0.0 ± 0.0
Trp
2.059TrpAla: 2.059 ± 0.469
0.158TrpCys: 0.158 ± 0.113
1.821TrpAsp: 1.821 ± 0.428
1.426TrpGlu: 1.426 ± 0.311
0.554TrpPhe: 0.554 ± 0.188
1.346TrpGly: 1.346 ± 0.309
0.475TrpHis: 0.475 ± 0.187
0.554TrpIle: 0.554 ± 0.203
1.03TrpLys: 1.03 ± 0.258
1.584TrpLeu: 1.584 ± 0.433
0.317TrpMet: 0.317 ± 0.187
0.792TrpAsn: 0.792 ± 0.248
1.663TrpPro: 1.663 ± 0.305
1.188TrpGln: 1.188 ± 0.264
1.426TrpArg: 1.426 ± 0.339
1.426TrpSer: 1.426 ± 0.371
1.188TrpThr: 1.188 ± 0.35
1.346TrpVal: 1.346 ± 0.275
0.475TrpTrp: 0.475 ± 0.173
0.475TrpTyr: 0.475 ± 0.174
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.247TyrAla: 3.247 ± 0.481
0.475TyrCys: 0.475 ± 0.212
1.505TyrAsp: 1.505 ± 0.371
1.663TyrGlu: 1.663 ± 0.382
0.475TyrPhe: 0.475 ± 0.2
3.009TyrGly: 3.009 ± 0.432
0.396TyrHis: 0.396 ± 0.157
0.95TyrIle: 0.95 ± 0.201
0.792TyrLys: 0.792 ± 0.21
1.901TyrLeu: 1.901 ± 0.425
0.238TyrMet: 0.238 ± 0.151
0.238TyrAsn: 0.238 ± 0.151
0.95TyrPro: 0.95 ± 0.277
1.188TyrGln: 1.188 ± 0.315
2.059TyrArg: 2.059 ± 0.441
1.426TyrSer: 1.426 ± 0.362
1.663TyrThr: 1.663 ± 0.3
1.346TyrVal: 1.346 ± 0.308
0.238TyrTrp: 0.238 ± 0.138
0.475TyrTyr: 0.475 ± 0.164
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (12628 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski