Amino acid dipepetide frequency for Microbacterium sp. cf046

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.692AlaAla: 20.692 ± 0.216
0.647AlaCys: 0.647 ± 0.027
8.738AlaAsp: 8.738 ± 0.094
8.145AlaGlu: 8.145 ± 0.107
4.046AlaPhe: 4.046 ± 0.06
12.131AlaGly: 12.131 ± 0.115
2.454AlaHis: 2.454 ± 0.05
6.467AlaIle: 6.467 ± 0.08
2.51AlaLys: 2.51 ± 0.055
13.821AlaLeu: 13.821 ± 0.136
2.696AlaMet: 2.696 ± 0.049
2.268AlaAsn: 2.268 ± 0.048
6.639AlaPro: 6.639 ± 0.087
3.73AlaGln: 3.73 ± 0.063
9.248AlaArg: 9.248 ± 0.114
7.537AlaSer: 7.537 ± 0.089
7.491AlaThr: 7.491 ± 0.091
11.434AlaVal: 11.434 ± 0.112
2.019AlaTrp: 2.019 ± 0.042
2.542AlaTyr: 2.542 ± 0.044
0.0AlaXaa: 0.0 ± 0.0
Cys
0.649CysAla: 0.649 ± 0.027
0.072CysCys: 0.072 ± 0.019
0.322CysAsp: 0.322 ± 0.016
0.259CysGlu: 0.259 ± 0.014
0.164CysPhe: 0.164 ± 0.013
0.507CysGly: 0.507 ± 0.022
0.115CysHis: 0.115 ± 0.01
0.201CysIle: 0.201 ± 0.012
0.056CysLys: 0.056 ± 0.007
0.422CysLeu: 0.422 ± 0.018
0.084CysMet: 0.084 ± 0.009
0.093CysAsn: 0.093 ± 0.01
0.258CysPro: 0.258 ± 0.016
0.112CysGln: 0.112 ± 0.01
0.315CysArg: 0.315 ± 0.016
0.298CysSer: 0.298 ± 0.017
0.331CysThr: 0.331 ± 0.018
0.421CysVal: 0.421 ± 0.018
0.073CysTrp: 0.073 ± 0.007
0.102CysTyr: 0.102 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
9.876AspAla: 9.876 ± 0.104
0.244AspCys: 0.244 ± 0.015
4.425AspAsp: 4.425 ± 0.08
4.504AspGlu: 4.504 ± 0.071
1.805AspPhe: 1.805 ± 0.042
6.269AspGly: 6.269 ± 0.101
1.232AspHis: 1.232 ± 0.029
2.553AspIle: 2.553 ± 0.048
0.932AspLys: 0.932 ± 0.027
6.3AspLeu: 6.3 ± 0.076
0.906AspMet: 0.906 ± 0.026
0.885AspAsn: 0.885 ± 0.029
4.535AspPro: 4.535 ± 0.079
1.615AspGln: 1.615 ± 0.039
4.737AspArg: 4.737 ± 0.067
2.765AspSer: 2.765 ± 0.05
2.809AspThr: 2.809 ± 0.049
5.524AspVal: 5.524 ± 0.068
1.036AspTrp: 1.036 ± 0.03
1.285AspTyr: 1.285 ± 0.036
0.0AspXaa: 0.0 ± 0.0
Glu
6.878GluAla: 6.878 ± 0.08
0.245GluCys: 0.245 ± 0.015
2.894GluAsp: 2.894 ± 0.052
2.88GluGlu: 2.88 ± 0.052
1.874GluPhe: 1.874 ± 0.042
4.116GluGly: 4.116 ± 0.064
1.415GluHis: 1.415 ± 0.033
2.762GluIle: 2.762 ± 0.052
1.213GluLys: 1.213 ± 0.036
6.621GluLeu: 6.621 ± 0.071
0.89GluMet: 0.89 ± 0.028
1.211GluAsn: 1.211 ± 0.037
3.233GluPro: 3.233 ± 0.063
2.119GluGln: 2.119 ± 0.052
5.182GluArg: 5.182 ± 0.079
3.09GluSer: 3.09 ± 0.047
2.967GluThr: 2.967 ± 0.05
4.552GluVal: 4.552 ± 0.068
1.035GluTrp: 1.035 ± 0.032
1.257GluTyr: 1.257 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
4.56PheAla: 4.56 ± 0.068
0.166PheCys: 0.166 ± 0.012
2.438PheAsp: 2.438 ± 0.045
1.848PheGlu: 1.848 ± 0.045
1.14PhePhe: 1.14 ± 0.033
3.444PheGly: 3.444 ± 0.057
0.591PheHis: 0.591 ± 0.021
1.367PheIle: 1.367 ± 0.033
0.417PheLys: 0.417 ± 0.022
2.904PheLeu: 2.904 ± 0.055
0.49PheMet: 0.49 ± 0.02
0.631PheAsn: 0.631 ± 0.026
1.478PhePro: 1.478 ± 0.039
0.776PheGln: 0.776 ± 0.027
1.833PheArg: 1.833 ± 0.039
1.82PheSer: 1.82 ± 0.042
2.23PheThr: 2.23 ± 0.044
2.921PheVal: 2.921 ± 0.054
0.553PheTrp: 0.553 ± 0.023
0.677PheTyr: 0.677 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
10.871GlyAla: 10.871 ± 0.117
0.562GlyCys: 0.562 ± 0.023
5.331GlyAsp: 5.331 ± 0.066
4.919GlyGlu: 4.919 ± 0.07
3.351GlyPhe: 3.351 ± 0.058
7.746GlyGly: 7.746 ± 0.104
1.866GlyHis: 1.866 ± 0.047
5.008GlyIle: 5.008 ± 0.075
1.931GlyLys: 1.931 ± 0.046
8.717GlyLeu: 8.717 ± 0.089
1.999GlyMet: 1.999 ± 0.04
1.614GlyAsn: 1.614 ± 0.05
4.002GlyPro: 4.002 ± 0.057
2.383GlyGln: 2.383 ± 0.042
6.428GlyArg: 6.428 ± 0.075
5.402GlySer: 5.402 ± 0.073
5.321GlyThr: 5.321 ± 0.076
7.794GlyVal: 7.794 ± 0.085
1.784GlyTrp: 1.784 ± 0.044
2.227GlyTyr: 2.227 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.356HisAla: 2.356 ± 0.046
0.112HisCys: 0.112 ± 0.01
1.321HisAsp: 1.321 ± 0.034
1.179HisGlu: 1.179 ± 0.028
0.612HisPhe: 0.612 ± 0.02
2.024HisGly: 2.024 ± 0.043
0.555HisHis: 0.555 ± 0.023
0.792HisIle: 0.792 ± 0.023
0.256HisLys: 0.256 ± 0.013
1.915HisLeu: 1.915 ± 0.038
0.324HisMet: 0.324 ± 0.016
0.34HisAsn: 0.34 ± 0.019
1.543HisPro: 1.543 ± 0.039
0.48HisGln: 0.48 ± 0.023
1.574HisArg: 1.574 ± 0.036
0.982HisSer: 0.982 ± 0.033
1.014HisThr: 1.014 ± 0.035
1.708HisVal: 1.708 ± 0.036
0.285HisTrp: 0.285 ± 0.017
0.43HisTyr: 0.43 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
7.727IleAla: 7.727 ± 0.086
0.244IleCys: 0.244 ± 0.013
3.734IleAsp: 3.734 ± 0.057
2.997IleGlu: 2.997 ± 0.052
1.311IlePhe: 1.311 ± 0.04
4.981IleGly: 4.981 ± 0.061
0.746IleHis: 0.746 ± 0.023
1.991IleIle: 1.991 ± 0.05
0.717IleLys: 0.717 ± 0.029
4.084IleLeu: 4.084 ± 0.066
0.644IleMet: 0.644 ± 0.021
0.841IleAsn: 0.841 ± 0.028
2.683IlePro: 2.683 ± 0.044
1.016IleGln: 1.016 ± 0.028
2.826IleArg: 2.826 ± 0.047
2.441IleSer: 2.441 ± 0.051
2.801IleThr: 2.801 ± 0.057
4.98IleVal: 4.98 ± 0.077
0.587IleTrp: 0.587 ± 0.023
0.845IleTyr: 0.845 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
2.229LysAla: 2.229 ± 0.047
0.06LysCys: 0.06 ± 0.007
1.036LysAsp: 1.036 ± 0.028
0.859LysGlu: 0.859 ± 0.032
0.433LysPhe: 0.433 ± 0.02
1.441LysGly: 1.441 ± 0.042
0.426LysHis: 0.426 ± 0.018
0.799LysIle: 0.799 ± 0.027
0.621LysLys: 0.621 ± 0.028
1.724LysLeu: 1.724 ± 0.042
0.316LysMet: 0.316 ± 0.017
0.422LysAsn: 0.422 ± 0.02
1.115LysPro: 1.115 ± 0.036
0.576LysGln: 0.576 ± 0.02
1.467LysArg: 1.467 ± 0.038
1.061LysSer: 1.061 ± 0.027
1.166LysThr: 1.166 ± 0.033
1.468LysVal: 1.468 ± 0.041
0.262LysTrp: 0.262 ± 0.017
0.4LysTyr: 0.4 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.296LeuAla: 14.296 ± 0.129
0.512LeuCys: 0.512 ± 0.019
6.666LeuAsp: 6.666 ± 0.083
5.488LeuGlu: 5.488 ± 0.074
2.956LeuPhe: 2.956 ± 0.055
9.07LeuGly: 9.07 ± 0.084
1.897LeuHis: 1.897 ± 0.04
4.884LeuIle: 4.884 ± 0.085
1.56LeuLys: 1.56 ± 0.037
9.849LeuLeu: 9.849 ± 0.128
1.657LeuMet: 1.657 ± 0.04
1.726LeuAsn: 1.726 ± 0.043
5.439LeuPro: 5.439 ± 0.065
2.466LeuGln: 2.466 ± 0.041
7.494LeuArg: 7.494 ± 0.101
5.75LeuSer: 5.75 ± 0.068
6.155LeuThr: 6.155 ± 0.07
8.971LeuVal: 8.971 ± 0.11
1.449LeuTrp: 1.449 ± 0.045
1.76LeuTyr: 1.76 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
2.143MetAla: 2.143 ± 0.045
0.098MetCys: 0.098 ± 0.01
0.854MetAsp: 0.854 ± 0.026
0.593MetGlu: 0.593 ± 0.025
0.56MetPhe: 0.56 ± 0.022
1.315MetGly: 1.315 ± 0.034
0.342MetHis: 0.342 ± 0.014
0.88MetIle: 0.88 ± 0.026
0.389MetLys: 0.389 ± 0.018
1.855MetLeu: 1.855 ± 0.042
0.312MetMet: 0.312 ± 0.018
0.478MetAsn: 0.478 ± 0.021
1.246MetPro: 1.246 ± 0.032
0.527MetGln: 0.527 ± 0.02
1.423MetArg: 1.423 ± 0.033
1.486MetSer: 1.486 ± 0.035
1.725MetThr: 1.725 ± 0.033
1.232MetVal: 1.232 ± 0.032
0.205MetTrp: 0.205 ± 0.013
0.312MetTyr: 0.312 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.397AsnAla: 2.397 ± 0.06
0.11AsnCys: 0.11 ± 0.01
1.151AsnAsp: 1.151 ± 0.033
0.89AsnGlu: 0.89 ± 0.03
0.605AsnPhe: 0.605 ± 0.021
1.881AsnGly: 1.881 ± 0.051
0.376AsnHis: 0.376 ± 0.018
0.802AsnIle: 0.802 ± 0.026
0.335AsnLys: 0.335 ± 0.018
1.808AsnLeu: 1.808 ± 0.039
0.323AsnMet: 0.323 ± 0.017
0.409AsnAsn: 0.409 ± 0.022
1.578AsnPro: 1.578 ± 0.037
0.527AsnGln: 0.527 ± 0.024
1.203AsnArg: 1.203 ± 0.032
0.952AsnSer: 0.952 ± 0.026
1.03AsnThr: 1.03 ± 0.034
1.602AsnVal: 1.602 ± 0.038
0.305AsnTrp: 0.305 ± 0.015
0.429AsnTyr: 0.429 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
7.397ProAla: 7.397 ± 0.099
0.187ProCys: 0.187 ± 0.013
4.27ProAsp: 4.27 ± 0.064
3.829ProGlu: 3.829 ± 0.059
1.844ProPhe: 1.844 ± 0.039
5.053ProGly: 5.053 ± 0.077
1.152ProHis: 1.152 ± 0.03
2.441ProIle: 2.441 ± 0.049
1.013ProLys: 1.013 ± 0.028
5.026ProLeu: 5.026 ± 0.065
0.917ProMet: 0.917 ± 0.027
1.028ProAsn: 1.028 ± 0.032
2.755ProPro: 2.755 ± 0.067
1.477ProGln: 1.477 ± 0.032
3.5ProArg: 3.5 ± 0.059
3.228ProSer: 3.228 ± 0.061
3.532ProThr: 3.532 ± 0.065
4.783ProVal: 4.783 ± 0.069
0.91ProTrp: 0.91 ± 0.027
1.112ProTyr: 1.112 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.278GlnAla: 3.278 ± 0.053
0.101GlnCys: 0.101 ± 0.008
1.351GlnAsp: 1.351 ± 0.031
1.244GlnGlu: 1.244 ± 0.035
0.915GlnPhe: 0.915 ± 0.03
1.969GlnGly: 1.969 ± 0.037
0.581GlnHis: 0.581 ± 0.022
1.372GlnIle: 1.372 ± 0.036
0.586GlnLys: 0.586 ± 0.021
3.162GlnLeu: 3.162 ± 0.054
0.487GlnMet: 0.487 ± 0.02
0.641GlnAsn: 0.641 ± 0.022
1.526GlnPro: 1.526 ± 0.035
1.043GlnGln: 1.043 ± 0.029
2.312GlnArg: 2.312 ± 0.048
1.486GlnSer: 1.486 ± 0.034
1.55GlnThr: 1.55 ± 0.038
2.26GlnVal: 2.26 ± 0.042
0.501GlnTrp: 0.501 ± 0.019
0.6GlnTyr: 0.6 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
9.135ArgAla: 9.135 ± 0.097
0.263ArgCys: 0.263 ± 0.015
4.475ArgAsp: 4.475 ± 0.069
4.267ArgGlu: 4.267 ± 0.06
2.486ArgPhe: 2.486 ± 0.047
5.528ArgGly: 5.528 ± 0.07
1.582ArgHis: 1.582 ± 0.042
3.787ArgIle: 3.787 ± 0.06
1.282ArgLys: 1.282 ± 0.034
7.484ArgLeu: 7.484 ± 0.096
1.719ArgMet: 1.719 ± 0.04
1.322ArgAsn: 1.322 ± 0.031
3.639ArgPro: 3.639 ± 0.055
1.9ArgGln: 1.9 ± 0.038
6.532ArgArg: 6.532 ± 0.107
4.167ArgSer: 4.167 ± 0.066
4.265ArgThr: 4.265 ± 0.057
6.117ArgVal: 6.117 ± 0.079
1.256ArgTrp: 1.256 ± 0.029
1.658ArgTyr: 1.658 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
7.24SerAla: 7.24 ± 0.092
0.253SerCys: 0.253 ± 0.015
3.422SerAsp: 3.422 ± 0.057
2.82SerGlu: 2.82 ± 0.049
2.004SerPhe: 2.004 ± 0.042
5.814SerGly: 5.814 ± 0.074
1.004SerHis: 1.004 ± 0.026
2.666SerIle: 2.666 ± 0.046
0.928SerLys: 0.928 ± 0.033
5.435SerLeu: 5.435 ± 0.076
1.112SerMet: 1.112 ± 0.029
1.049SerAsn: 1.049 ± 0.027
3.242SerPro: 3.242 ± 0.053
1.421SerGln: 1.421 ± 0.038
3.783SerArg: 3.783 ± 0.056
3.352SerSer: 3.352 ± 0.063
3.632SerThr: 3.632 ± 0.054
4.866SerVal: 4.866 ± 0.064
0.94SerTrp: 0.94 ± 0.035
1.159SerTyr: 1.159 ± 0.031
0.0SerXaa: 0.0 ± 0.0
Thr
7.778ThrAla: 7.778 ± 0.086
0.277ThrCys: 0.277 ± 0.019
3.751ThrAsp: 3.751 ± 0.062
3.007ThrGlu: 3.007 ± 0.051
1.918ThrPhe: 1.918 ± 0.042
5.735ThrGly: 5.735 ± 0.076
1.129ThrHis: 1.129 ± 0.031
3.036ThrIle: 3.036 ± 0.056
1.1ThrLys: 1.1 ± 0.031
5.93ThrLeu: 5.93 ± 0.077
0.985ThrMet: 0.985 ± 0.028
1.092ThrAsn: 1.092 ± 0.032
3.968ThrPro: 3.968 ± 0.074
1.441ThrGln: 1.441 ± 0.034
3.656ThrArg: 3.656 ± 0.053
3.315ThrSer: 3.315 ± 0.065
3.744ThrThr: 3.744 ± 0.067
5.621ThrVal: 5.621 ± 0.084
0.889ThrTrp: 0.889 ± 0.025
1.189ThrTyr: 1.189 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
11.557ValAla: 11.557 ± 0.117
0.488ValCys: 0.488 ± 0.02
5.746ValAsp: 5.746 ± 0.071
4.819ValGlu: 4.819 ± 0.065
2.917ValPhe: 2.917 ± 0.05
7.08ValGly: 7.08 ± 0.08
1.674ValHis: 1.674 ± 0.042
4.758ValIle: 4.758 ± 0.068
1.412ValLys: 1.412 ± 0.039
9.028ValLeu: 9.028 ± 0.1
1.462ValMet: 1.462 ± 0.031
1.729ValAsn: 1.729 ± 0.042
4.736ValPro: 4.736 ± 0.065
2.189ValGln: 2.189 ± 0.047
6.278ValArg: 6.278 ± 0.08
4.863ValSer: 4.863 ± 0.06
5.63ValThr: 5.63 ± 0.074
8.459ValVal: 8.459 ± 0.104
1.255ValTrp: 1.255 ± 0.038
1.546ValTyr: 1.546 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
1.714TrpAla: 1.714 ± 0.039
0.086TrpCys: 0.086 ± 0.009
0.933TrpAsp: 0.933 ± 0.034
0.679TrpGlu: 0.679 ± 0.023
0.626TrpPhe: 0.626 ± 0.025
1.185TrpGly: 1.185 ± 0.033
0.35TrpHis: 0.35 ± 0.016
0.826TrpIle: 0.826 ± 0.032
0.305TrpLys: 0.305 ± 0.016
1.836TrpLeu: 1.836 ± 0.048
0.38TrpMet: 0.38 ± 0.019
0.461TrpAsn: 0.461 ± 0.021
0.772TrpPro: 0.772 ± 0.026
0.61TrpGln: 0.61 ± 0.021
1.334TrpArg: 1.334 ± 0.035
1.026TrpSer: 1.026 ± 0.032
1.009TrpThr: 1.009 ± 0.025
1.247TrpVal: 1.247 ± 0.033
0.421TrpTrp: 0.421 ± 0.02
0.318TrpTyr: 0.318 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.48TyrAla: 2.48 ± 0.051
0.127TyrCys: 0.127 ± 0.01
1.378TyrAsp: 1.378 ± 0.039
1.207TyrGlu: 1.207 ± 0.034
0.72TyrPhe: 0.72 ± 0.022
1.966TyrGly: 1.966 ± 0.044
0.317TyrHis: 0.317 ± 0.015
0.805TyrIle: 0.805 ± 0.023
0.318TyrLys: 0.318 ± 0.019
2.122TyrLeu: 2.122 ± 0.041
0.274TyrMet: 0.274 ± 0.014
0.473TyrAsn: 0.473 ± 0.021
1.066TyrPro: 1.066 ± 0.032
0.559TyrGln: 0.559 ± 0.02
1.739TyrArg: 1.739 ± 0.043
1.12TyrSer: 1.12 ± 0.029
1.159TyrThr: 1.159 ± 0.031
1.666TyrVal: 1.666 ± 0.038
0.352TyrTrp: 0.352 ± 0.017
0.497TyrTyr: 0.497 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3641 proteins (1231243 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski