Treponema porcinum

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Treponemataceae; Treponema

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2189 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4JIX1|A0A1T4JIX1_9SPIO Protein involved in polysaccharide export contains SLBB domain of the beta-grasp fold OS=Treponema porcinum OX=261392 GN=SAMN02745149_00377 PE=4 SV=1
MM1 pKa = 7.55LKK3 pKa = 10.34LRR5 pKa = 11.84NAVLTALLAFCLAMPFVSCSFNDD28 pKa = 4.06GATGDD33 pKa = 3.84VSNLVCDD40 pKa = 3.98VSNLVTEE47 pKa = 4.32QTGNNQNGGNGGSTGGNEE65 pKa = 4.11KK66 pKa = 10.47PSGGDD71 pKa = 3.19EE72 pKa = 4.52DD73 pKa = 5.52SGDD76 pKa = 3.72NGNEE80 pKa = 3.96NPSGGNNGGSGNEE93 pKa = 4.01NPSGGNEE100 pKa = 4.14KK101 pKa = 9.04PTDD104 pKa = 3.72PTEE107 pKa = 4.47PGAGTTYY114 pKa = 10.53VVIYY118 pKa = 10.08NGDD121 pKa = 3.78IIEE124 pKa = 4.38EE125 pKa = 4.16SLPEE129 pKa = 4.18SMWFYY134 pKa = 11.18GVEE137 pKa = 4.02EE138 pKa = 4.42VNLEE142 pKa = 4.04EE143 pKa = 4.89GVDD146 pKa = 3.67YY147 pKa = 11.04TMKK150 pKa = 10.78GNEE153 pKa = 3.55ITLTYY158 pKa = 10.39SGYY161 pKa = 10.75QKK163 pKa = 10.65VLPLLEE169 pKa = 4.38SSGGDD174 pKa = 2.95GGQEE178 pKa = 4.06VIPPVTDD185 pKa = 3.96GPVTDD190 pKa = 5.04GPVPDD195 pKa = 4.08VPGDD199 pKa = 3.62GKK201 pKa = 10.11GTYY204 pKa = 7.99TVMYY208 pKa = 9.46NGQPLVDD215 pKa = 3.89EE216 pKa = 4.69NGEE219 pKa = 4.39VVTIPAAALDD229 pKa = 4.32YY230 pKa = 10.46YY231 pKa = 11.25KK232 pKa = 10.85SILSEE237 pKa = 3.76GDD239 pKa = 3.19YY240 pKa = 10.47TISGNVITLTEE251 pKa = 4.04SGFNKK256 pKa = 10.13LNKK259 pKa = 10.01LSAAGSGAGEE269 pKa = 3.98

Molecular weight: 27.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4JN56|A0A1T4JN56_9SPIO Uncharacterized protein OS=Treponema porcinum OX=261392 GN=SAMN02745149_00618 PE=4 SV=1
MM1 pKa = 7.77TDD3 pKa = 3.54FNKK6 pKa = 10.36IINLVLEE13 pKa = 4.5STLQTLQMVSLSTLFSVMLGLPLGILLCISDD44 pKa = 4.29PQSGIKK50 pKa = 8.99PRR52 pKa = 11.84RR53 pKa = 11.84VLYY56 pKa = 10.22QVLTRR61 pKa = 11.84IVNALRR67 pKa = 11.84SFPFIILMVLLFPLSRR83 pKa = 11.84LIVGTSIGTAATIVPLSIAAAPFVARR109 pKa = 11.84VIEE112 pKa = 4.2TALKK116 pKa = 10.21EE117 pKa = 3.86VDD119 pKa = 3.75LGVIQAARR127 pKa = 11.84AMGSTTMQIILKK139 pKa = 9.64VLLPEE144 pKa = 4.68AMPSLVDD151 pKa = 4.02GVTLTVINLIGYY163 pKa = 8.91SAMAGALGGGGLGDD177 pKa = 3.37LAIRR181 pKa = 11.84YY182 pKa = 7.61GYY184 pKa = 10.1QRR186 pKa = 11.84FRR188 pKa = 11.84PEE190 pKa = 3.22VMAIAVVVILLMVEE204 pKa = 4.83LIQFAGTFVSRR215 pKa = 11.84RR216 pKa = 11.84ISARR220 pKa = 11.84RR221 pKa = 3.27

Molecular weight: 23.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2189

0

2189

754806

40

3783

344.8

38.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.858 ± 0.055

1.522 ± 0.023

5.595 ± 0.045

7.048 ± 0.062

4.948 ± 0.049

6.595 ± 0.06

1.488 ± 0.02

7.11 ± 0.049

7.139 ± 0.048

8.664 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.025

4.821 ± 0.045

3.561 ± 0.028

3.045 ± 0.033

4.178 ± 0.04

7.14 ± 0.051

5.574 ± 0.064

6.659 ± 0.044

0.921 ± 0.02

3.742 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski