CRESS virus sp. ctYls24

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; unclassified Cressdnaviricota; CRESS viruses

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WB88|A0A5Q2WB88_9VIRU Uncharacterized protein OS=CRESS virus sp. ctYls24 OX=2656679 PE=4 SV=1
MM1 pKa = 7.19NFSVEE6 pKa = 5.9LFYY9 pKa = 11.02QWLLEE14 pKa = 3.98EE15 pKa = 4.15EE16 pKa = 4.72VINEE20 pKa = 4.09EE21 pKa = 4.22EE22 pKa = 4.27LTLEE26 pKa = 4.97DD27 pKa = 5.46LIEE30 pKa = 4.75DD31 pKa = 4.06LEE33 pKa = 4.52GMAKK37 pKa = 9.85EE38 pKa = 3.96WDD40 pKa = 3.82EE41 pKa = 4.04EE42 pKa = 4.43AEE44 pKa = 4.98SIGQLSKK51 pKa = 11.1EE52 pKa = 3.71RR53 pKa = 11.84LAFQDD58 pKa = 3.61PKK60 pKa = 11.49

Molecular weight: 7.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WAL2|A0A5Q2WAL2_9VIRU ATP-dependent helicase Rep OS=CRESS virus sp. ctYls24 OX=2656679 PE=3 SV=1
MM1 pKa = 7.46AFRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84GYY8 pKa = 10.0KK9 pKa = 9.45RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84TYY14 pKa = 10.21PRR16 pKa = 11.84RR17 pKa = 11.84SYY19 pKa = 10.92RR20 pKa = 11.84RR21 pKa = 11.84LRR23 pKa = 11.84RR24 pKa = 11.84YY25 pKa = 8.52GKK27 pKa = 10.22RR28 pKa = 11.84MGRR31 pKa = 11.84RR32 pKa = 11.84GGKK35 pKa = 9.66YY36 pKa = 7.54RR37 pKa = 11.84TTVQRR42 pKa = 11.84AIGFSRR48 pKa = 11.84SQIVKK53 pKa = 8.72MRR55 pKa = 11.84YY56 pKa = 8.15VQQLQLNPVAGSYY69 pKa = 10.71AYY71 pKa = 10.12AIYY74 pKa = 10.16SANGIYY80 pKa = 10.59NPVVSRR86 pKa = 11.84SAGVGFSTDD95 pKa = 3.6HH96 pKa = 6.32QPISYY101 pKa = 9.49DD102 pKa = 2.93QWQIAYY108 pKa = 10.31NDD110 pKa = 3.57YY111 pKa = 9.6TVIGSKK117 pKa = 9.77MSIVANPPNSTNPSVGSGIIAIQLSDD143 pKa = 3.49SASPVVVPTTVMEE156 pKa = 4.46SGRR159 pKa = 11.84AAYY162 pKa = 8.69KK163 pKa = 10.31QLATNTSGNSVRR175 pKa = 11.84LRR177 pKa = 11.84KK178 pKa = 9.96GFSAKK183 pKa = 10.3KK184 pKa = 8.84FWRR187 pKa = 11.84LQSIKK192 pKa = 10.7DD193 pKa = 3.64NQKK196 pKa = 10.83SIGSGFGTNPSEE208 pKa = 3.9QAYY211 pKa = 9.53YY212 pKa = 9.88WLEE215 pKa = 3.75FFTNDD220 pKa = 4.27SSGSTNAGPFVWVTIDD236 pKa = 3.44YY237 pKa = 7.83TVLLTGPADD246 pKa = 3.71LTQSS250 pKa = 3.27

Molecular weight: 27.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

658

60

282

164.5

19.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.927 ± 0.665

1.368 ± 0.83

4.559 ± 1.226

6.839 ± 3.542

4.863 ± 2.308

7.143 ± 1.033

1.824 ± 0.84

5.471 ± 0.138

7.599 ± 2.398

6.535 ± 2.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.976 ± 0.188

4.103 ± 0.599

3.191 ± 0.778

4.407 ± 1.31

6.991 ± 1.621

7.751 ± 1.902

5.471 ± 1.164

5.927 ± 0.657

2.736 ± 0.907

5.319 ± 0.765
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski