Reston ebolavirus (strain Philippines-96) (REBOV) (Reston Ebola virus)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Filoviridae; Ebolavirus; Reston ebolavirus

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6G8D4|C6G8D4_EBORE Polymerase cofactor VP35 OS=Reston ebolavirus (strain Philippines-96) OX=129003 GN=VP35 PE=3 SV=1
MM1 pKa = 7.99DD2 pKa = 5.45RR3 pKa = 11.84GTRR6 pKa = 11.84RR7 pKa = 11.84IWVSQNQGDD16 pKa = 3.88TDD18 pKa = 4.39LDD20 pKa = 3.56YY21 pKa = 11.56HH22 pKa = 7.15KK23 pKa = 10.48ILTAGLTVQQGIVRR37 pKa = 11.84QKK39 pKa = 9.98IISVYY44 pKa = 10.68LVDD47 pKa = 5.17NLEE50 pKa = 4.29AMCQLVIQAFEE61 pKa = 4.02AGIDD65 pKa = 3.8FQEE68 pKa = 4.51NADD71 pKa = 3.95SFLLMLCLHH80 pKa = 7.05HH81 pKa = 7.47AYY83 pKa = 10.35QGDD86 pKa = 3.72YY87 pKa = 11.36KK88 pKa = 11.22LFLEE92 pKa = 4.76SNAVQYY98 pKa = 11.38LEE100 pKa = 3.83GHH102 pKa = 5.42GFKK105 pKa = 10.86FEE107 pKa = 3.85LRR109 pKa = 11.84KK110 pKa = 9.73RR111 pKa = 11.84DD112 pKa = 3.34GVNRR116 pKa = 11.84LEE118 pKa = 4.25EE119 pKa = 4.48LLPAATSGKK128 pKa = 10.03NIRR131 pKa = 11.84RR132 pKa = 11.84TLAALPEE139 pKa = 4.38EE140 pKa = 4.68EE141 pKa = 4.28TTEE144 pKa = 4.05ANAGQFLSFASLFLPKK160 pKa = 10.39LVVGEE165 pKa = 4.31KK166 pKa = 10.46ACLEE170 pKa = 4.0KK171 pKa = 10.81VQRR174 pKa = 11.84QIQVHH179 pKa = 6.09AEE181 pKa = 3.78QGLIQYY187 pKa = 7.32PTAWQSVGHH196 pKa = 5.52MMVIFRR202 pKa = 11.84LMRR205 pKa = 11.84TNFLIKK211 pKa = 10.49YY212 pKa = 8.44LLIHH216 pKa = 6.27QGMHH220 pKa = 5.09MVAGHH225 pKa = 7.11DD226 pKa = 3.8ANDD229 pKa = 3.41AVIANSVAQARR240 pKa = 11.84FSGLLIVKK248 pKa = 8.23TVLDD252 pKa = 5.01HH253 pKa = 7.39ILQKK257 pKa = 9.72TDD259 pKa = 2.92QGVRR263 pKa = 11.84LHH265 pKa = 6.47PLARR269 pKa = 11.84TAKK272 pKa = 9.66VRR274 pKa = 11.84NEE276 pKa = 3.59VNAFKK281 pKa = 10.73AALSSLAKK289 pKa = 9.86HH290 pKa = 6.16GEE292 pKa = 3.99YY293 pKa = 10.9APFARR298 pKa = 11.84LLNLSGVNNLEE309 pKa = 4.28HH310 pKa = 6.95GLYY313 pKa = 9.39PQLSAIALGVATAHH327 pKa = 6.58GSTLAGVNVGEE338 pKa = 4.64QYY340 pKa = 10.17QQLRR344 pKa = 11.84EE345 pKa = 4.06AATEE349 pKa = 4.1AEE351 pKa = 4.44KK352 pKa = 10.74QLQQYY357 pKa = 10.35AEE359 pKa = 4.07SRR361 pKa = 11.84EE362 pKa = 4.03LDD364 pKa = 3.64SLGLDD369 pKa = 3.46DD370 pKa = 4.17QEE372 pKa = 5.77RR373 pKa = 11.84RR374 pKa = 11.84ILMNFHH380 pKa = 5.78QKK382 pKa = 10.38KK383 pKa = 10.33NEE385 pKa = 3.58ISFQQTNAMVTLRR398 pKa = 11.84KK399 pKa = 9.42EE400 pKa = 3.98RR401 pKa = 11.84LAKK404 pKa = 9.22LTEE407 pKa = 5.05AITLASRR414 pKa = 11.84PNLGSRR420 pKa = 11.84QDD422 pKa = 4.28DD423 pKa = 3.84DD424 pKa = 5.23NEE426 pKa = 4.3IPFPGPISNNPDD438 pKa = 3.09QDD440 pKa = 3.83HH441 pKa = 7.06LEE443 pKa = 4.66DD444 pKa = 5.3DD445 pKa = 5.55PIDD448 pKa = 3.92SRR450 pKa = 11.84DD451 pKa = 3.7TIIPNSAIDD460 pKa = 3.97PEE462 pKa = 6.58DD463 pKa = 3.98GDD465 pKa = 3.99FEE467 pKa = 4.89NYY469 pKa = 9.93NGYY472 pKa = 10.03HH473 pKa = 6.96DD474 pKa = 5.79DD475 pKa = 3.95EE476 pKa = 5.6VGTAGDD482 pKa = 4.33LVLFDD487 pKa = 5.92LDD489 pKa = 4.89DD490 pKa = 5.52HH491 pKa = 7.1EE492 pKa = 7.02DD493 pKa = 3.58DD494 pKa = 4.35NKK496 pKa = 11.11AFEE499 pKa = 4.85PQDD502 pKa = 3.77SSPQSQRR509 pKa = 11.84GIEE512 pKa = 4.16RR513 pKa = 11.84EE514 pKa = 3.99RR515 pKa = 11.84LIHH518 pKa = 7.05PPLGNNKK525 pKa = 9.69DD526 pKa = 3.83DD527 pKa = 5.38NRR529 pKa = 11.84ASDD532 pKa = 4.34NNQQSADD539 pKa = 3.72SEE541 pKa = 4.42EE542 pKa = 4.28QEE544 pKa = 4.63DD545 pKa = 4.67RR546 pKa = 11.84YY547 pKa = 10.89NRR549 pKa = 11.84HH550 pKa = 6.53RR551 pKa = 11.84GPEE554 pKa = 3.57RR555 pKa = 11.84TTANRR560 pKa = 11.84RR561 pKa = 11.84LSPVYY566 pKa = 10.61EE567 pKa = 4.37EE568 pKa = 5.05DD569 pKa = 3.54TLIDD573 pKa = 3.99QGDD576 pKa = 4.55DD577 pKa = 3.97DD578 pKa = 5.26PSGPPPLEE586 pKa = 5.09SDD588 pKa = 4.64DD589 pKa = 5.49DD590 pKa = 4.38DD591 pKa = 5.89ASSSQQDD598 pKa = 3.31PDD600 pKa = 3.7YY601 pKa = 10.78TAVAPPAPVYY611 pKa = 10.41RR612 pKa = 11.84SAEE615 pKa = 3.92AHH617 pKa = 6.03EE618 pKa = 5.07PPHH621 pKa = 6.56KK622 pKa = 10.58SSNEE626 pKa = 3.72PAEE629 pKa = 4.13TSQLNEE635 pKa = 4.9DD636 pKa = 4.09PDD638 pKa = 3.84IGQSKK643 pKa = 10.55SMQKK647 pKa = 10.52LEE649 pKa = 4.16EE650 pKa = 4.54TYY652 pKa = 10.66HH653 pKa = 6.32HH654 pKa = 7.49LLRR657 pKa = 11.84TQGPFEE663 pKa = 5.17AINYY667 pKa = 7.02YY668 pKa = 11.1HH669 pKa = 6.55MMKK672 pKa = 10.36DD673 pKa = 3.3EE674 pKa = 4.28PVIFSTDD681 pKa = 3.16DD682 pKa = 3.41GKK684 pKa = 11.48EE685 pKa = 3.69YY686 pKa = 10.01TYY688 pKa = 10.98PDD690 pKa = 4.17SLEE693 pKa = 3.82EE694 pKa = 5.44AYY696 pKa = 9.9PPWLTEE702 pKa = 4.38KK703 pKa = 10.6EE704 pKa = 4.05RR705 pKa = 11.84LDD707 pKa = 3.43NEE709 pKa = 3.66NRR711 pKa = 11.84YY712 pKa = 9.84IYY714 pKa = 10.47INNQQFFWPVMSPRR728 pKa = 11.84DD729 pKa = 3.55KK730 pKa = 10.89FLAILQHH737 pKa = 5.87HH738 pKa = 5.42QQ739 pKa = 3.02

Molecular weight: 83.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6G8E0|C6G8E0_EBORE RNA-directed RNA polymerase L OS=Reston ebolavirus (strain Philippines-96) OX=129003 GN=L PE=4 SV=1
MM1 pKa = 7.54AKK3 pKa = 9.15ATGRR7 pKa = 11.84YY8 pKa = 9.46NLVPPKK14 pKa = 10.39KK15 pKa = 10.33DD16 pKa = 3.2MEE18 pKa = 4.78KK19 pKa = 10.98GVIFSDD25 pKa = 3.86LCNFLITQTLQGWKK39 pKa = 9.93VYY41 pKa = 8.75WAGIEE46 pKa = 3.96FDD48 pKa = 3.79VSQKK52 pKa = 10.62GMALLTRR59 pKa = 11.84LKK61 pKa = 10.22TNDD64 pKa = 3.91FAPAWAMTRR73 pKa = 11.84NLFPHH78 pKa = 7.3LFQNPNSVIQSPIWALRR95 pKa = 11.84VILAAGLQDD104 pKa = 3.53QLLDD108 pKa = 3.66HH109 pKa = 6.71SLVEE113 pKa = 4.38PLTGALGLISDD124 pKa = 4.63WLLTTTSTHH133 pKa = 5.48FNLRR137 pKa = 11.84TRR139 pKa = 11.84SVKK142 pKa = 10.57DD143 pKa = 3.33QLSLRR148 pKa = 11.84MLSLIRR154 pKa = 11.84SNILQFINKK163 pKa = 9.16LDD165 pKa = 3.88ALHH168 pKa = 5.76VVNYY172 pKa = 10.4NGLLSSIEE180 pKa = 4.31IGTSTHH186 pKa = 6.34TIIITRR192 pKa = 11.84TNMGFLVEE200 pKa = 4.12VQEE203 pKa = 4.34PDD205 pKa = 3.21KK206 pKa = 11.48SAMNSKK212 pKa = 10.49RR213 pKa = 11.84PGPVKK218 pKa = 10.57FSLLHH223 pKa = 5.82EE224 pKa = 4.6SAFKK228 pKa = 10.72PFTRR232 pKa = 11.84VQQSGMQSLIMEE244 pKa = 4.99FNSLLAII251 pKa = 4.51

Molecular weight: 28.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

1

8

5201

251

2212

650.1

73.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.922 ± 0.505

1.654 ± 0.263

5.345 ± 0.587

5.191 ± 0.556

3.922 ± 0.395

5.345 ± 0.418

2.884 ± 0.275

5.96 ± 0.456

5.114 ± 0.299

10.998 ± 0.689

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.711 ± 0.17

5.364 ± 0.291

5.922 ± 0.881

5.095 ± 0.375

5.153 ± 0.331

7.922 ± 0.398

6.768 ± 0.714

5.114 ± 0.297

1.346 ± 0.212

3.269 ± 0.563
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski